Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009158

Physical Location & Seq

Genomic Coordinates Forward (+)

91955954 .. 91964468

Gene Structure 8515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00155550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGATTGCGTCCCTCTTTTGAAGGGTATGTTCAAGAAAGCCAATAAAGAACTGAAATTGGAGTCCAAGGAAATCGATAGGAGGAAGTACAATCTGCTGAGGAGGAGGCAGATTAAGGCGGAGACGGAGGCGTGGGAGAAGATGGTGGATGAGTATAAGGACTTGGAGAGAGTAATGCGTGAGAAGAAGCTGGCTCCGAGCTTGCCTTATGTAAAGGCCTTGTTTTTGGGGTGGTTTGAGCCACTGAGGGAGGCCATTGCTAAGGAGCAGAAGGCACAGCAAACCAAGAAGCACAAGGCAGCGTTTGCGCCGCACATTGATTTGTTGCCGCCGGATAAGATGGCACTGATTGTAATGCATAAGCTGATGGGGTTGGTGATGATGGGGAATCAAGATGGGTGTGTTCAGGTGGTTCAGGCTGCCGTGCACATTGGTATGGCCATAGAGCAGGAGGTTCGGATTCACAGTTTCTTGGAGAAGACTAAAAGTCTCCAGAGAAAGAAGACTGCGGCTGCGGATGAAGATGGTCTGAGCAAGGAGAAGGAGATACTGAGGAAACGTGTTAATGGTTTGATTAGAAGGAAAAGACTGGTTGAGGTGCAAAAGCTACTGAAAAAAGAAGATATGAAGCCCTGGGGTCGGGATACACAGGCTAAGCTGGGAAGTCGGCTGATAGAACTATTAACAGAAACAGCTCATGTACAACCTCCACTTGACCAATTGGCAGATGGTCCACCTGATGTTAGACCTGCATTTAGGCACAGACTAAAGGCTGCGGCAAAAAGTCCAGGGCAGAAACTTGTGAAGAACTATGGAGTTATTGAATGTGATCCGCTAGTTCTTACCGGACTTGATAAAACTGCTAAACATATGTTGATTCCTTATGTGCCAATGTTGGTGCCTCCCAAAAGATGGAAAGGGTATGACAAGGGTGGACACTTGTTCCTACCTTCTTATGTCATGCGTACCCATGGATCTAGGAAGCAGGTAGATACAATGAGAAATATTCCTGGAAGACAGATGCAGAAAGTATTTGAGGTATATAAGCCCCTCGATATGCTTGGAAGCACCAAATGGCGGATTAATAAAAAGGTTCTTAACGTGGTGGAGAGCATCTGGGCTAGAGGTGGCAACATTGCAGGCCTGGTTGATCGTGAAGATGTTCCTGTACCAGAGAAACCACCATCTGAGGATTTAACAGAAATTCAGGAATGGAGATGGAGTGCAAGAAAAGCAAAGAAGCTTAACCAAGAGAGGCATTCTCAACGATTCCTTGAATTTGCTGAAGGGCGGCCATTGGGGAAGTCCGGATTGCGATGGCTGAAGATTCATTTAGCAAACTTGTATTCAGGTGGTGTTGAAAAGCTTTCATACGATGGGCGTATAGCATTCGTGGATAACCACATTGATGATATATTTGATTCAGCAACAAACCCTGTTAATGGAAATCGTTGGTGGTTAACTGCTGAAGATCCTTTACAGTGCCTAGCTGCTTGTATCAATCTATCAGAAGCCTTAAATAGCCCATCACCACATACTGTCATTTCTCATTTACCCATCCATCAGTTGGAAGCAGCTGCAGTCAACTTAGTTGCTGGAGAGAAACCTGCTGATGTGTATTCAGAAATAGCTGTGAGGGTACATGACATTATGAAGAGAGACAGCAATAAGGACCCAACTACCAGCCCAAATGCTTTATTGGCTAGAGTCTTAGTCGATCAGATTGATCGGAAATTGGTGAAACAGACTGTGATGACTTCAGTATATGGTGTTACTTACGTTGGGGCGCGTGAACAGATAAAAAGAAGATTAGAAGAGAAGGGTCTTATAACTGATGATAGACTATTGTTCACCGCAGCTTGTTATGCTGCTAAAGTGACACTGGCTGCCCTTGGAGAGATTTTCCAAGCTGCACGTGGCATAATGAGCTGGCTTGGTGATTGTGCCAAGGTAATTGCTTCAGAAAATCAGCCTGTTTGTTGGACGACTCCTCTAGGTCTTCCTGTTGTGCAACCATACTGTAAAAGTGAACGACATCTTGATGCTTGCACCGAGGGTTTGATGTACCTCTATATGATACCTTTGTGCTCTTCTCATCATTTGCTCACATTCTTCTTGGATGGCACTGGCATGGGCACTGGAAATGGAAAATGTTTCAAAACTATTTTGGATATGGTTGCTAAGAGTTTACCAATACACCTTATAAGAACATCCCTTCAGGTTCTGGCCTTGCAGCGGGAGAGTAACTTGGTAGATGTAAGGAAACAGAGAACGGCATTTCCTCCAAATTTTGTACACTCACTTGACGGTTCACACATGATGATGACAGCTCTTGCCTGCAGGGATGCTGGCATACGCTTTGCAGGGGTGCATGACTCCTTCTGGACTCATGCATGTGACGTGGATCAAATGAACCAGATACTTCGGGAAAACTTTGTGGAGCTCTATAGCAAGCCGATACTTGAGAATTTGCTTAAAAGCTTTCAAGCAGCGTATCCAGAATTGACGTTTCCTCCCCTCCCGGAAAGAGGTGACTTTGACTTGCAAGAGGTTCCACATTTTAGAGGTGAACGATGTACAGTAAGGGAGACTCTTCTTCTGAAGTTCACGGTTCAAGATCTATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000959 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006390 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034062 GO:0034245 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:0097747 GO:0098798 GO:0140053 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RNA_pol RPOL_N

Protein Analysis

875

Amino Acids

99.18

Weight (kDa)

9.27

Isoelectric Point (pI)

46.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPOL_N	PF14700	36 - 356	4.1e-72	DNA-directed RNA polymerase N-terminal
RNA_pol	PF00940	431 - 679	3e-82	DNA-dependent RNA polymerase
RNA_pol	PF00940	734 - 833	1.4e-42	DNA-dependent RNA polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24120 AT2G24120
fragaria_vesca	FvH4_1g00590 FvH4_1g00590 FvH4_1g00590 FvH4_1g00590
malus_domestica	MD02G1004800.v1.1
prunus_persica	Prupe.7G266200_v2.0.a1 Prupe.7G266200_v2.0.a1
pyrus_communis	pycom02g00340 pycom15g13310
rosa_chinensis	RchiOBHm_Chr2g0085221
rosa_laevigata	RLG00000015668
rosa_multiflora	Rmu_sc0008510.1_g000002 Rmu_sc0011989.1_g000007
rosa_roxburghii	Rroxscaffold_2G00155550
rosa_rugosa	Rorug01G0458400
rosa_samantha	Rh2AG006900 Rh2BG007500 Rh2CG008300 Rh2DG008600
rosa_wichuraiana	Rw2G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1829, 2204
Acc36I	ACCTGC	3 cut(s)	757, 976, 1615
AccB1I	GGYRCC	1 cut(s)	898
AccB7I	CCANNNNNTGG	2 cut(s)	912, 1567
AccII	CGCG	1 cut(s)	1789
AccIII	TCCGGA	1 cut(s)	1307
AclWI	GGATC	4 cut(s)	824, 982, 1466, 2412
AcoI	YGGCCR	2 cut(s)	438, 1292
AcsI	RAATTY	4 cut(s)	1203, 1277, 2287, 2467
AcuI	CTGAAG	7 cut(s)	1305, 1343, 1488, 1743, 1944, 2201, 2621
AcvI	CACGTG	1 cut(s)	1916
AfaI	GTAC	8 cut(s)	89, 702, 967, 1170, 1641, 2066, 2295, 2578
AfiI	CCNNNNNNNGG	7 cut(s)	639, 912, 1077, 1442, 1567, 2235, 2528
AflIII	ACRYGT	1 cut(s)	559
AgsI	TTSAA	8 cut(s)	22, 34, 824, 1277, 1361, 2158, 2486, 2615
AhdI	GACNNNNNGTC	1 cut(s)	486
AjiI	CACGTC	1 cut(s)	2401
AjnI	CCWGG	4 cut(s)	632, 787, 1009, 1143
AloI	GAACNNNNNNTCC	2 cut(s)	2263, 2295
Alw21I	GWGCWC	3 cut(s)	429, 2090, 2445
Alw26I	GTCTC	4 cut(s)	116, 494, 1653, 2582
Alw44I	GTGCAC	1 cut(s)	425
AlwI	GGATC	4 cut(s)	824, 982, 1466, 2412
AlwNI	CAGNNNCTG	1 cut(s)	2224
Aor13HI	TCCGGA	1 cut(s)	1307
AoxI	GGCC	6 cut(s)	216, 252, 438, 1141, 1292, 2226
ApaLI	GTGCAC	1 cut(s)	425
ApoI	RAATTY	4 cut(s)	1203, 1277, 2287, 2467
AseI	ATTAAT	1 cut(s)	1083
Asp700I	GAANNNNTTC	1 cut(s)	1161
AspLEI	GCGC	2 cut(s)	310, 1789
AspS9I	GGNCC	2 cut(s)	731, 1672
AsuC2I	CCSGG	1 cut(s)	2522
AsuHPI	GGTGA	7 cut(s)	388, 1521, 1750, 1843, 1949, 2543, 2579
AvaII	GGWCC	2 cut(s)	731, 1672
BaeGI	GKGCMC	2 cut(s)	429, 2138
BaeI	ACNNNNGTAYC	2 cut(s)	2069, 2102
BalI	TGGCCA	1 cut(s)	440
BanI	GGYRCC	1 cut(s)	898
BanII	GRGCYC	1 cut(s)	2445
BarI	GAAGNNNNNNTAC	2 cut(s)	1461, 1493
BbrPI	CACGTG	1 cut(s)	1916
BbsI	GAAGAC	4 cut(s)	485, 509, 1021, 1991
Bbv12I	GWGCWC	3 cut(s)	429, 2090, 2445
BbvCI	CCTCAGC	1 cut(s)	98
BceAI	ACGGC	2 cut(s)	407, 2289
BcgI	CGANNNNNNTGC	2 cut(s)	437, 471
BciT130I	CCWGG	4 cut(s)	634, 789, 1011, 1145
BciVI	GTATCC	2 cut(s)	637, 2505
BcnI	CCSGG	1 cut(s)	2522
BcoDI	GTCTC	4 cut(s)	116, 494, 1653, 2582
BfaI	CTAG	6 cut(s)	836, 978, 1122, 1487, 1704, 1994
BfmI	CTRYAG	3 cut(s)	1578, 2338, 2446
BfuAI	ACCTGC	3 cut(s)	757, 976, 1615
BfuI	GTATCC	2 cut(s)	637, 2505
BglII	AGATCT	1 cut(s)	2617
BlpI	GCTNAGC	1 cut(s)	654
Bme1390I	CCNGG	5 cut(s)	634, 789, 1011, 1145, 2522
Bme18I	GGWCC	2 cut(s)	731, 1672
BmeRI	GACNNNNNGTC	1 cut(s)	486
BmgBI	CACGTC	1 cut(s)	2401
BmgT120I	GGNCC	2 cut(s)	731, 1672
BmiI	GGNNCC	4 cut(s)	195, 900, 1674, 2553
BmrFI	CCNGG	5 cut(s)	634, 789, 1011, 1145, 2522
BmsI	GCATC	4 cut(s)	1011, 1122, 2032, 2335
BpiI	GAAGAC	4 cut(s)	485, 509, 1021, 1991
BplI	GAGNNNNNCTC	2 cut(s)	1246, 1278
BpmI	CTGGAG	2 cut(s)	476, 1617
Bpu10I	CCTNAGC	2 cut(s)	98, 261
Bpu1102I	GCTNAGC	1 cut(s)	654
BpuEI	CTTGAG	1 cut(s)	2483
BpuMI	CCSGG	1 cut(s)	2522
Bsa29I	ATCGAT	1 cut(s)	75
BsaAI	YACGTR	1 cut(s)	1916
BsaJI	CCNNGG	8 cut(s)	66, 632, 633, 788, 970, 1891, 1947, 2052
BsaWI	WCCGGW	2 cut(s)	845, 1307
BsaXI	ACNNNNNCTCC	4 cut(s)	1207, 1237, 2497, 2527
Bsc4I	CCNNNNNNNGG	7 cut(s)	639, 912, 1077, 1442, 1567, 2235, 2528
Bse1I	ACTGG	4 cut(s)	594, 1887, 2131, 2143
Bse3DI	GCAATG	2 cut(s)	255, 1134
BseAI	TCCGGA	1 cut(s)	1307
BseBI	CCWGG	4 cut(s)	634, 789, 1011, 1145
BseCI	ATCGAT	1 cut(s)	75
BseDI	CCNNGG	8 cut(s)	66, 632, 633, 788, 970, 1891, 1947, 2052
BseGI	GGATG	6 cut(s)	154, 523, 1557, 2125, 2210, 2350
BseLI	CCNNNNNNNGG	7 cut(s)	639, 912, 1077, 1442, 1567, 2235, 2528
BseMI	GCAATG	2 cut(s)	255, 1134
BseMII	CTCAG	5 cut(s)	89, 236, 521, 542, 1179
BseNI	ACTGG	4 cut(s)	594, 1887, 2131, 2143
BseRI	GAGGAG	3 cut(s)	115, 118, 1980
BseSI	GKGCMC	2 cut(s)	429, 2138
BseYI	CCCAGC	1 cut(s)	658
BsgI	GTGCAG	1 cut(s)	1896
Bsh1236I	CGCG	1 cut(s)	1789
BshFI	GGCC	6 cut(s)	218, 254, 440, 1143, 1294, 2228
BshNI	GGYRCC	1 cut(s)	898
BshVI	ATCGAT	1 cut(s)	75
BsiHKAI	GWGCWC	3 cut(s)	429, 2090, 2445
BsiSI	CCGG	4 cut(s)	332, 846, 1308, 2522
BslI	CCNNNNNNNGG	7 cut(s)	639, 912, 1077, 1442, 1567, 2235, 2528
BsmAI	GTCTC	4 cut(s)	116, 494, 1653, 2582
BsmBI	CGTCTC	1 cut(s)	116
BsmI	GAATGC	2 cut(s)	1258, 1388
BsnI	GGCC	6 cut(s)	218, 254, 440, 1143, 1294, 2228
Bsp1286I	GDGCHC	4 cut(s)	429, 2090, 2138, 2445
Bsp13I	TCCGGA	1 cut(s)	1307
Bsp1407I	TGTACA	3 cut(s)	700, 2293, 2576
Bsp143I	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
Bsp1720I	GCTNAGC	1 cut(s)	654
Bsp19I	CCATGG	1 cut(s)	970
BspANI	GGCC	6 cut(s)	218, 254, 440, 1143, 1294, 2228
BspCNI	CTCAG	5 cut(s)	90, 237, 522, 543, 1180
BspDI	ATCGAT	1 cut(s)	75
BspEI	TCCGGA	1 cut(s)	1307
BspFNI	CGCG	1 cut(s)	1789
BspLI	GGNNCC	4 cut(s)	195, 900, 1674, 2553
BspMAI	CTGCAG	2 cut(s)	1582, 2342
BspMI	ACCTGC	3 cut(s)	757, 976, 1615
BspPI	GGATC	4 cut(s)	824, 982, 1466, 2412
BspQI	GCTCTTC	1 cut(s)	2095
BspT107I	GGYRCC	1 cut(s)	898
BsrDI	GCAATG	2 cut(s)	255, 1134
BsrGI	TGTACA	3 cut(s)	700, 2293, 2576
BsrI	ACTGG	4 cut(s)	594, 1887, 2131, 2143
BssECI	CCNNGG	8 cut(s)	66, 632, 633, 788, 970, 1891, 1947, 2052
BssMI	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
BssT1I	CCWWGG	4 cut(s)	66, 970, 1891, 1947
Bst2UI	CCWGG	4 cut(s)	634, 789, 1011, 1145
Bst4CI	ACNGT	8 cut(s)	467, 1482, 1540, 1750, 2021, 2309, 2581, 2611
Bst6I	CTCTTC	4 cut(s)	1649, 1809, 2095, 2598
BstAPI	GCANNNNNTGC	1 cut(s)	305
BstAUI	TGTACA	3 cut(s)	700, 2293, 2576
BstBAI	YACGTR	1 cut(s)	1916
BstC8I	GCNNGC	8 cut(s)	192, 203, 1141, 1931, 2047, 2338, 2350, 2453
BstDSI	CCRYGG	1 cut(s)	970
BstF5I	GGATG	6 cut(s)	154, 523, 1557, 2125, 2210, 2350
BstFNI	CGCG	1 cut(s)	1789
BstHHI	GCGC	2 cut(s)	310, 1789
BstKTI	GATC	8 cut(s)	832, 977, 1153, 1474, 1720, 1729, 2407, 2620
BstMAI	GTCTC	4 cut(s)	116, 494, 1653, 2582
BstMBI	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
BstMWI	GCNNNNNNNGC	5 cut(s)	305, 1232, 1700, 1865, 1926
BstNI	CCWGG	4 cut(s)	634, 789, 1011, 1145
BstNSI	RCATGY	1 cut(s)	2397
BstSCI	CCNGG	5 cut(s)	632, 787, 1009, 1143, 2520
BstSFI	CTRYAG	3 cut(s)	1578, 2338, 2446
BstSLI	GKGCMC	2 cut(s)	429, 2138
BstUI	CGCG	1 cut(s)	1789
BstV2I	GAAGAC	4 cut(s)	485, 509, 1021, 1991
BstX2I	RGATCY	3 cut(s)	974, 1471, 2617
BstYI	RGATCY	3 cut(s)	974, 1471, 2617
Bsu15I	ATCGAT	1 cut(s)	75
BsuI	GTATCC	2 cut(s)	637, 2505
BsuRI	GGCC	6 cut(s)	218, 254, 440, 1143, 1294, 2228
BsuTUI	ATCGAT	1 cut(s)	75
BtgI	CCRYGG	1 cut(s)	970
BtgZI	GCGATG	1 cut(s)	1330
BtrI	CACGTC	1 cut(s)	2401
BtsCI	GGATG	6 cut(s)	154, 523, 1557, 2125, 2210, 2350
BtsIMutI	CAGTG	6 cut(s)	242, 344, 1487, 1880, 2124, 2136
BveI	ACCTGC	3 cut(s)	757, 976, 1615
Cac8I	GCNNGC	8 cut(s)	192, 203, 1141, 1931, 2047, 2338, 2350, 2453
CaiI	CAGNNNCTG	1 cut(s)	2224
CfoI	GCGC	2 cut(s)	310, 1789
Cfr13I	GGNCC	2 cut(s)	731, 1672
ClaI	ATCGAT	1 cut(s)	75
Csp6I	GTAC	8 cut(s)	88, 701, 966, 1169, 1640, 2065, 2294, 2577
CviAII	CATG	9 cut(s)	698, 961, 971, 1643, 2131, 2317, 2372, 2390, 2394
CviQI	GTAC	8 cut(s)	88, 701, 966, 1169, 1640, 2065, 2294, 2577
DpnI	GATC	8 cut(s)	831, 976, 1152, 1473, 1719, 1728, 2406, 2619
DpnII	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
DriI	GACNNNNNGTC	1 cut(s)	486
EaeI	YGGCCR	2 cut(s)	438, 1292
Eam1104I	CTCTTC	4 cut(s)	1649, 1809, 2095, 2598
Eam1105I	GACNNNNNGTC	1 cut(s)	486
EarI	CTCTTC	4 cut(s)	1649, 1809, 2095, 2598
EciI	GGCGGA	2 cut(s)	134, 1093
Ecl136II	GAGCTC	1 cut(s)	2443
Eco130I	CCWWGG	4 cut(s)	66, 970, 1891, 1947
Eco147I	AGGCCT	2 cut(s)	218, 1143
Eco24I	GRGCYC	1 cut(s)	2445
Eco47I	GGWCC	2 cut(s)	731, 1672
Eco53kI	GAGCTC	1 cut(s)	2443
Eco57I	CTGAAG	7 cut(s)	1305, 1343, 1488, 1743, 1944, 2201, 2621
Eco72I	CACGTG	1 cut(s)	1916
EcoICRI	GAGCTC	1 cut(s)	2443
EcoO109I	RGGNCCY	1 cut(s)	1672
EcoRII	CCWGG	4 cut(s)	632, 787, 1009, 1143
EcoT14I	CCWWGG	4 cut(s)	66, 970, 1891, 1947
EcoT22I	ATGCAT	2 cut(s)	360, 2395
EcoT38I	GRGCYC	1 cut(s)	2445
ErhI	CCWWGG	4 cut(s)	66, 970, 1891, 1947
Esp3I	CGTCTC	1 cut(s)	116
FaeI	CATG	9 cut(s)	701, 964, 974, 1646, 2134, 2320, 2375, 2393, 2397
FatI	CATG	9 cut(s)	697, 960, 970, 1642, 2130, 2316, 2371, 2389, 2393
FauI	CCCGC	1 cut(s)	2229
FauNDI	CATATG	1 cut(s)	870
FokI	GGATG	6 cut(s)	161, 530, 1544, 2132, 2197, 2357
FriOI	GRGCYC	1 cut(s)	2445
FspBI	CTAG	6 cut(s)	836, 978, 1122, 1487, 1704, 1994
GlaI	GCGC	2 cut(s)	309, 1788
GsaI	CCCAGC	1 cut(s)	662
GsuI	CTGGAG	2 cut(s)	476, 1617
HaeIII	GGCC	6 cut(s)	218, 254, 440, 1143, 1294, 2228
HapII	CCGG	4 cut(s)	332, 846, 1308, 2522
HhaI	GCGC	2 cut(s)	310, 1789
Hin1II	CATG	9 cut(s)	701, 964, 974, 1646, 2134, 2320, 2375, 2393, 2397
Hin6I	GCGC	2 cut(s)	308, 1787
HinP1I	GCGC	2 cut(s)	308, 1787
HincII	GTYRAC	2 cut(s)	1461, 1585
HindII	GTYRAC	2 cut(s)	1461, 1585
HindIII	AAGCTT	3 cut(s)	1241, 1364, 2479
HpaI	GTTAAC	1 cut(s)	1461
HpaII	CCGG	4 cut(s)	332, 846, 1308, 2522
HphI	GGTGA	7 cut(s)	388, 1521, 1750, 1843, 1949, 2543, 2579
HpyAV	CCTTC	9 cut(s)	16, 265, 535, 573, 960, 1280, 1813, 2225, 2389
HpyCH4III	ACNGT	8 cut(s)	467, 1482, 1540, 1750, 2021, 2309, 2581, 2611
HpyCH4IV	ACGT	6 cut(s)	559, 1101, 1779, 1915, 2400, 2507
HpyF10VI	GCNNNNNNNGC	5 cut(s)	305, 1232, 1700, 1865, 1926
HpySE526I	ACGT	6 cut(s)	559, 1101, 1779, 1915, 2400, 2507
Hsp92II	CATG	9 cut(s)	701, 964, 974, 1646, 2134, 2320, 2375, 2393, 2397
HspAI	GCGC	2 cut(s)	308, 1787
Kpn2I	TCCGGA	1 cut(s)	1307
KspAI	GTTAAC	1 cut(s)	1461
Kzo9I	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
LguI	GCTCTTC	1 cut(s)	2095
LmnI	GCTCC	3 cut(s)	199, 265, 2440
LweI	GCATC	4 cut(s)	1011, 1122, 2032, 2335
MaeI	CTAG	6 cut(s)	836, 978, 1122, 1487, 1704, 1994
MaeII	ACGT	6 cut(s)	559, 1101, 1779, 1915, 2400, 2507
MaeIII	GTNAC	5 cut(s)	1771, 1876, 2243, 2396, 2531
MalI	GATC	8 cut(s)	831, 976, 1152, 1473, 1719, 1728, 2406, 2619
MboI	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
MfeI	CAATTG	1 cut(s)	719
MflI	RGATCY	3 cut(s)	974, 1471, 2617
MhlI	GDGCHC	4 cut(s)	429, 2090, 2138, 2445
MlsI	TGGCCA	1 cut(s)	440
MluCI	AATT	9 cut(s)	56, 719, 1203, 1277, 1733, 1953, 2287, 2467, 2501
MluNI	TGGCCA	1 cut(s)	440
MlyI	GAGTC	6 cut(s)	71, 1716, 1981, 2369, 2380, 2584
MmeI	TCCRAC	2 cut(s)	1548, 1961
Mox20I	TGGCCA	1 cut(s)	440
Mph1103I	ATGCAT	2 cut(s)	360, 2395
MroI	TCCGGA	1 cut(s)	1307
MroXI	GAANNNNTTC	1 cut(s)	1161
MscI	TGGCCA	1 cut(s)	440
MslI	CAYNNNNRTG	6 cut(s)	434, 1407, 2040, 2129, 2321, 2394
Msp20I	TGGCCA	1 cut(s)	440
MspA1I	CMGCKG	2 cut(s)	1577, 2236
MspI	CCGG	4 cut(s)	332, 846, 1308, 2522
MspR9I	CCNGG	5 cut(s)	634, 789, 1011, 1145, 2522
MunI	CAATTG	1 cut(s)	719
Mva1269I	GAATGC	2 cut(s)	1258, 1388
MvaI	CCWGG	4 cut(s)	634, 789, 1011, 1145
MvnI	CGCG	1 cut(s)	1789
MwoI	GCNNNNNNNGC	5 cut(s)	305, 1232, 1700, 1865, 1926
NciI	CCSGG	1 cut(s)	2522
NcoI	CCATGG	1 cut(s)	970
NdeI	CATATG	1 cut(s)	870
NdeII	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
NlaIII	CATG	9 cut(s)	701, 964, 974, 1646, 2134, 2320, 2375, 2393, 2397
NlaIV	GGNNCC	4 cut(s)	195, 900, 1674, 2553
NmuCI	GTSAC	3 cut(s)	1876, 2396, 2531
NsiI	ATGCAT	2 cut(s)	360, 2395
NspI	RCATGY	1 cut(s)	2397
PasI	CCCWGGG	1 cut(s)	633
PceI	AGGCCT	2 cut(s)	218, 1143
PciSI	GCTCTTC	1 cut(s)	2095
PctI	GAATGC	2 cut(s)	1258, 1388
PdmI	GAANNNNTTC	1 cut(s)	1161
PfeI	GAWTC	6 cut(s)	388, 460, 877, 1269, 1327, 1421
PflMI	CCANNNNNTGG	2 cut(s)	912, 1567
PfoI	TCCNGGA	2 cut(s)	1009, 2520
PleI	GAGTC	6 cut(s)	70, 1715, 1981, 2369, 2380, 2584
PmaCI	CACGTG	1 cut(s)	1916
PmlI	CACGTG	1 cut(s)	1916
PpsI	GAGTC	6 cut(s)	70, 1715, 1981, 2369, 2380, 2584
Ppu21I	YACGTR	1 cut(s)	1916
PpuMI	RGGWCCY	1 cut(s)	1672
PshBI	ATTAAT	1 cut(s)	1083
PsiI	TTATAA	2 cut(s)	1829, 2204
Psp124BI	GAGCTC	1 cut(s)	2445
Psp5II	RGGWCCY	1 cut(s)	1672
Psp6I	CCWGG	4 cut(s)	632, 787, 1009, 1143
PspCI	CACGTG	1 cut(s)	1916
PspFI	CCCAGC	1 cut(s)	658
PspGI	CCWGG	4 cut(s)	632, 787, 1009, 1143
PspN4I	GGNNCC	4 cut(s)	195, 900, 1674, 2553
PspPI	GGNCC	2 cut(s)	731, 1672
PspPPI	RGGWCCY	1 cut(s)	1672
PstI	CTGCAG	2 cut(s)	1582, 2342
PstNI	CAGNNNCTG	1 cut(s)	2224
PsuI	RGATCY	3 cut(s)	974, 1471, 2617
PvuII	CAGCTG	1 cut(s)	1577
RsaI	GTAC	8 cut(s)	89, 702, 967, 1170, 1641, 2066, 2295, 2578
RsaNI	GTAC	8 cut(s)	88, 701, 966, 1169, 1640, 2065, 2294, 2577
RseI	CAYNNNNRTG	6 cut(s)	434, 1407, 2040, 2129, 2321, 2394
SacI	GAGCTC	1 cut(s)	2445
SapI	GCTCTTC	1 cut(s)	2095
Sau3AI	GATC	8 cut(s)	829, 974, 1150, 1471, 1717, 1726, 2404, 2617
Sau96I	GGNCC	2 cut(s)	731, 1672
SbfI	CCTGCAGG	1 cut(s)	2342
SchI	GAGTC	6 cut(s)	71, 1716, 1981, 2369, 2380, 2584
ScrFI	CCNGG	5 cut(s)	634, 789, 1011, 1145, 2522
SdaI	CCTGCAGG	1 cut(s)	2342
SduI	GDGCHC	4 cut(s)	429, 2090, 2138, 2445
SfaNI	GCATC	4 cut(s)	1011, 1122, 2032, 2335
SfcI	CTRYAG	3 cut(s)	1578, 2338, 2446
SinI	GGWCC	2 cut(s)	731, 1672
SmiMI	CAYNNNNRTG	6 cut(s)	434, 1407, 2040, 2129, 2321, 2394
SmlI	CTYRAG	1 cut(s)	2462
SmoI	CTYRAG	1 cut(s)	2462
Sse8387I	CCTGCAGG	1 cut(s)	2342
Sse9I	AATT	9 cut(s)	56, 719, 1203, 1277, 1733, 1953, 2287, 2467, 2501
SseBI	AGGCCT	2 cut(s)	218, 1143
SspI	AATATT	1 cut(s)	1006
SspMI	CTAG	6 cut(s)	836, 978, 1122, 1487, 1704, 1994
SstI	GAGCTC	1 cut(s)	2445
StuI	AGGCCT	2 cut(s)	218, 1143
StyD4I	CCNGG	5 cut(s)	632, 787, 1009, 1143, 2520
StyI	CCWWGG	4 cut(s)	66, 970, 1891, 1947
TaaI	ACNGT	8 cut(s)	467, 1482, 1540, 1750, 2021, 2309, 2581, 2611
TaiI	ACGT	6 cut(s)	562, 1104, 1782, 1918, 2403, 2510
TaqI	TCGA	3 cut(s)	75, 1053, 1716
TasI	AATT	9 cut(s)	56, 719, 1203, 1277, 1733, 1953, 2287, 2467, 2501
TatI	WGTACW	4 cut(s)	87, 700, 2293, 2576
TauI	GCSGC	5 cut(s)	313, 331, 512, 779, 1294
TfiI	GAWTC	6 cut(s)	388, 460, 877, 1269, 1327, 1421
TscAI	CASTG	6 cut(s)	249, 351, 1487, 1887, 2131, 2143
TseFI	GTSAC	3 cut(s)	1876, 2396, 2531
Tsp45I	GTSAC	3 cut(s)	1876, 2396, 2531
TspDTI	ATGAA	6 cut(s)	534, 641, 1319, 1359, 1667, 2426
TspGWI	ACGGA	1 cut(s)	140
TspRI	CASTG	6 cut(s)	249, 351, 1487, 1887, 2131, 2143
Van91I	CCANNNNNTGG	2 cut(s)	912, 1567
VneI	GTGCAC	1 cut(s)	425
VpaK11BI	GGWCC	2 cut(s)	731, 1672
VspI	ATTAAT	1 cut(s)	1083
XapI	RAATTY	4 cut(s)	1203, 1277, 2287, 2467
XceI	RCATGY	1 cut(s)	2397
XcmI	CCANNNNNNNNNTGG	3 cut(s)	725, 1564, 1913
XmnI	GAANNNNTTC	1 cut(s)	1161
XspI	CTAG	6 cut(s)	836, 978, 1122, 1487, 1704, 1994
Zsp2I	ATGCAT	2 cut(s)	360, 2395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.