MD02G1005000.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006173
Physical Location & Seq
Reverse (-)
352684 .. 355491
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005000.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGCGGAGGGTGGTGTGCCTTGTGGAATGCCTGAAACTGATATGATAACGCTAACCGAAGCGCTTAGAGCACAACAACAACTACTGCAAAAGCTTTACTCTGATCTGGATAAGGAAAGAGAAGCATCATCTACTGCAGCTGATGAAGCTCTATCCATGATACTGCGTTTGCAAGGAGAAAAGTCCGCTATGAAGATGGAAGCAAATCAATACAAGAGATTGGCGGAGGAAAAGATCTGCCATGCCGAGGAAGCTCTTGCCATTTTCGAAGACCTCATTTATCAGAAAGAAATGGAAACTGCTTCGCTTGAGTTCCAACTTCAGGCTTATAGGTACAAGCTTTTAAGCATGGGCTGCAGCACGGAGCTAGGCGCTGGTGAAAATGTATATCCGGAGAATTTGTTGTTTCAAAGAAATGATTACGGAAGTGGGGAAGGGGGTGTTAGTGGGACGATTAGAAGAATCAACTCTCGAGGTGATTTTGGCAATGGGGAAACGGGTGTTAGCGAGGCTATTAGAAGAATCAACTCTCTACCTCCACTTGAGCTCAAGGAATTGCTCAAAAGCACTGTGGAAAGAGATAGAGATAAAGACAGAGATAAAGAAAGAGAACGAGAAAGAGACAGACCAATGATCCCCAAACCCGAACCTGCAACAAAAAAACAGGAGAAGAAGGTGGATCAGGAAGTCAATGTCCAGAGCGTAGATGTCGAGAAGAAATCACCAAATTATGCAGGTGGGAACATGAATATTATCTGGGAGCAGATTAAGAAGTTAGACGCACGAGTGAAGGAGATATCCGATTGTAAGGATTATGGCAAATCTGCACTCTGGAAGGGTCCTCCATCCAGGACTTGTTCATTGCCTCCAATTCCAAAGATAAGCATAAGCCCAAAAAATGAGCCAGCCAGTGAAGAAATGATTGCTAGTTTGGATCAACTTAAGCAGAGTGAGAATCTACCGGGAAGGGATGGCCCTTCATGCTCCTCAAGAGTCCATGATGTTTTCGAAGTCCCACAAAGTTATGAGAATCATAAAGCCGGTGAAGGAGAGAAGAAAGAACTTAATACATTGACTCTGGAAGTCGAGAACAGGCTTGGTAAGCCGCATCTGGTGATGGATGAGAAGAGTGAAGCATATGTTAGAGATGAAACAGATAGGGTAAAGAAAATGTTGCATATCAAGAAGCAGGAGAACAAAATACCTACACCAAGAGATGGGATAAGTAGTGTTGACTCGAATCTGAGAGAGAGAGGGGTGAGTGGTGGCGTTACTGAATCTCAGCAAGCAAAATTTCAACAGTTGTGGAGGAGGATAGAGCGTCTGGAGGGAGAGAGGAATAATATAAGGCAAGAAATTAGTCATGCGGGAGAACAGGAGTTGAAGCTGTTCCGGGAGATACATGAGCATCTTAATTTAATACAGTGTGACATGCGTAGTTGGAAGCCTAAGAAACCCTCTCCACAGGATGATGAGCCCTTGCACAATGTTATGGAGGCAATGCTGCACTTTTGGCTTTGA

Protein Analysis

507

Amino Acids

57.67

Weight (kDa)

5.66

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 17 - 107 1.3e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 725
Acc36I ACCTGC 2 cut(s) 658, 725
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 5 cut(s) 5, 186, 224, 1106, 1367
AclWI GGATC 3 cut(s) 628, 687, 942
AcsI RAATTY 2 cut(s) 397, 1292
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 1 cut(s) 335
AfeI AGCGCT 1 cut(s) 63
AfiI CCNNNNNNNGG 3 cut(s) 247, 322, 1217
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 3 cut(s) 410, 1298, 1384
AjnI CCWGG 1 cut(s) 848
AluBI AGCT 8 cut(s) 94, 140, 149, 254, 340, 367, 547, 1387
AluI AGCT 8 cut(s) 94, 140, 149, 254, 340, 367, 547, 1387
Alw21I GWGCWC 2 cut(s) 73, 549
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 3 cut(s) 628, 687, 942
Ama87I CYCGRG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 391
Aor51HI AGCGCT 1 cut(s) 63
AoxI GGCC 1 cut(s) 973
ApeKI GCWGC 4 cut(s) 137, 354, 357, 1504
ApoI RAATTY 2 cut(s) 397, 1292
Asp700I GAANNNNTTC 1 cut(s) 1388
AspLEI GCGC 2 cut(s) 64, 374
AspS9I GGNCC 2 cut(s) 839, 974
AsuC2I CCSGG 2 cut(s) 963, 1394
AsuHPI GGTGA 6 cut(s) 389, 488, 714, 1055, 1126, 1270
AsuII TTCGAA 2 cut(s) 267, 1008
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 1 cut(s) 839
BanII GRGCYC 2 cut(s) 549, 1479
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 73, 549
BbvI GCAGC 4 cut(s) 149, 341, 369, 1491
BccI CCATC 5 cut(s) 190, 853, 965, 1111, 1211
BciT130I CCWGG 1 cut(s) 850
BcnI CCSGG 2 cut(s) 963, 1394
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 2 cut(s) 368, 927
BfmI CTRYAG 2 cut(s) 135, 355
BfoI RGCGCY 2 cut(s) 65, 375
BfrI CTTAAG 1 cut(s) 941
BfuAI ACCTGC 2 cut(s) 658, 725
BglII AGATCT 1 cut(s) 234
BisI GCNGC 5 cut(s) 138, 355, 358, 1106, 1505
BlsI GCNGC 5 cut(s) 139, 356, 359, 1107, 1506
Bme1390I CCNGG 3 cut(s) 850, 963, 1394
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 839, 974
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 3 cut(s) 850, 963, 1394
BmsI GCATC 3 cut(s) 134, 1117, 1417
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 1346
Bpu14I TTCGAA 2 cut(s) 267, 1008
BpuEI CTTGAG 4 cut(s) 329, 533, 563, 973
BpuMI CCSGG 2 cut(s) 963, 1394
BsaJI CCNNGG 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 322, 1217
Bse118I RCCGGY 1 cut(s) 1040
Bse1I ACTGG 1 cut(s) 909
Bse3DI GCAATG 3 cut(s) 493, 860, 1506
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 1 cut(s) 850
BseDI CCNNGG 1 cut(s) 246
BseGI GGATG 4 cut(s) 845, 976, 1126, 1474
BseLI CCNNNNNNNGG 3 cut(s) 247, 322, 1217
BseMI GCAATG 3 cut(s) 493, 860, 1506
BseMII CTCAG 2 cut(s) 1235, 1295
BseNI ACTGG 1 cut(s) 909
BseRI GAGGAG 2 cut(s) 976, 1324
BseXI GCAGC 4 cut(s) 149, 341, 369, 1491
BsgI GTGCAG 2 cut(s) 810, 1490
BshFI GGCC 1 cut(s) 975
BsiHKAI GWGCWC 2 cut(s) 73, 549
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 4 cut(s) 392, 962, 1041, 1393
BslFI GGGAC 2 cut(s) 463, 998
BslI CCNNNNNNNGG 3 cut(s) 247, 322, 1217
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 2 cut(s) 463, 998
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 1 cut(s) 975
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 2 cut(s) 267, 1008
Bsp1286I GDGCHC 3 cut(s) 73, 549, 1479
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 5 cut(s) 103, 234, 633, 679, 934
BspACI CCGC 5 cut(s) 5, 186, 224, 1106, 1367
BspANI GGCC 1 cut(s) 975
BspCNI CTCAG 2 cut(s) 1236, 1294
BspEI TCCGGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 840
BspMAI CTGCAG 2 cut(s) 139, 359
BspMI ACCTGC 2 cut(s) 658, 725
BspPI GGATC 3 cut(s) 628, 687, 942
BspT104I TTCGAA 2 cut(s) 267, 1008
BspTI CTTAAG 1 cut(s) 941
BsrDI GCAATG 3 cut(s) 493, 860, 1506
BsrFI RCCGGY 1 cut(s) 1040
BsrI ACTGG 1 cut(s) 909
BssAI RCCGGY 1 cut(s) 1040
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 5 cut(s) 103, 234, 633, 679, 934
BssSI CACGAG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 850
Bst4CI ACNGT 3 cut(s) 571, 1302, 1425
Bst6I CTCTTC 1 cut(s) 1121
BstAFI CTTAAG 1 cut(s) 941
BstAPI GCANNNNNTGC 1 cut(s) 354
BstBI TTCGAA 2 cut(s) 267, 1008
BstC8I GCNNGC 2 cut(s) 906, 1287
BstDEI CTNAG 4 cut(s) 65, 1244, 1281, 1449
BstF5I GGATG 4 cut(s) 845, 976, 1126, 1474
BstH2I RGCGCY 2 cut(s) 65, 375
BstHHI GCGC 2 cut(s) 64, 374
BstKTI GATC 5 cut(s) 106, 237, 636, 682, 937
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 5 cut(s) 103, 234, 633, 679, 934
BstMWI GCNNNNNNNGC 7 cut(s) 68, 146, 251, 354, 981, 1102, 1513
BstNI CCWGG 1 cut(s) 850
BstNSI RCATGY 1 cut(s) 1435
BstSCI CCNGG 3 cut(s) 848, 961, 1392
BstSFI CTRYAG 2 cut(s) 135, 355
BstV1I GCAGC 4 cut(s) 149, 341, 369, 1491
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 1 cut(s) 975
BtsCI GGATG 4 cut(s) 845, 976, 1126, 1474
BtsIMutI CAGTG 3 cut(s) 567, 916, 1430
BveI ACCTGC 2 cut(s) 658, 725
Cac8I GCNNGC 2 cut(s) 906, 1287
CfoI GCGC 2 cut(s) 64, 374
Cfr10I RCCGGY 1 cut(s) 1040
Cfr13I GGNCC 2 cut(s) 839, 974
CseI GACGC 2 cut(s) 788, 1310
Csp6I GTAC 1 cut(s) 334
CspCI CAANNNNNGTGG 2 cut(s) 1286, 1321
CviAII CATG 9 cut(s) 157, 242, 349, 745, 981, 998, 1364, 1403, 1432
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 4 cut(s) 65, 1244, 1281, 1449
DpnI GATC 5 cut(s) 105, 236, 635, 681, 936
DpnII GATC 5 cut(s) 103, 234, 633, 679, 934
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
EciI GGCGGA 2 cut(s) 20, 239
Ecl136II GAGCTC 1 cut(s) 547
Eco24I GRGCYC 2 cut(s) 549, 1479
Eco32I GATATC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 839
Eco47III AGCGCT 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 848
EcoRV GATATC 1 cut(s) 798
EcoT38I GRGCYC 2 cut(s) 549, 1479
FaeI CATG 9 cut(s) 160, 245, 352, 748, 984, 1001, 1367, 1406, 1435
FaqI GGGAC 2 cut(s) 463, 998
FatI CATG 9 cut(s) 156, 241, 348, 744, 980, 997, 1363, 1402, 1431
FauI CCCGC 1 cut(s) 1360
FauNDI CATATG 1 cut(s) 1138
Fnu4HI GCNGC 5 cut(s) 138, 355, 358, 1106, 1505
FokI GGATG 4 cut(s) 832, 983, 1133, 1481
FriOI GRGCYC 2 cut(s) 549, 1479
Fsp4HI GCNGC 5 cut(s) 138, 355, 358, 1106, 1505
FspBI CTAG 2 cut(s) 368, 927
GlaI GCGC 2 cut(s) 63, 373
GluI GCNGC 5 cut(s) 138, 355, 358, 1106, 1505
GsuI CTGGAG 1 cut(s) 1346
HaeII RGCGCY 2 cut(s) 65, 375
HaeIII GGCC 1 cut(s) 975
HapII CCGG 4 cut(s) 392, 962, 1041, 1393
HgaI GACGC 2 cut(s) 788, 1310
HhaI GCGC 2 cut(s) 64, 374
Hin1II CATG 9 cut(s) 160, 245, 352, 748, 984, 1001, 1367, 1406, 1435
Hin6I GCGC 2 cut(s) 62, 372
HinP1I GCGC 2 cut(s) 62, 372
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HindIII AAGCTT 2 cut(s) 92, 338
HinfI GANTC 9 cut(s) 462, 522, 955, 993, 1030, 1075, 1235, 1240, 1277
HpaII CCGG 4 cut(s) 392, 962, 1041, 1393
HphI GGTGA 6 cut(s) 389, 488, 714, 1055, 1126, 1270
Hpy166II GTNNAC 1 cut(s) 1234
Hpy188I TCNGA 4 cut(s) 103, 285, 802, 1245
Hpy8I GTNNAC 1 cut(s) 1234
HpyAV CCTTC 7 cut(s) 428, 667, 784, 829, 960, 987, 1040
HpyCH4III ACNGT 3 cut(s) 571, 1302, 1425
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 146, 251, 354, 981, 1102, 1513
HpyF3I CTNAG 4 cut(s) 65, 1244, 1281, 1449
Hsp92II CATG 9 cut(s) 160, 245, 352, 748, 984, 1001, 1367, 1406, 1435
HspAI GCGC 2 cut(s) 62, 372
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 5 cut(s) 103, 234, 633, 679, 934
LmnI GCTCC 3 cut(s) 364, 760, 989
Lsp1109I GCAGC 4 cut(s) 149, 341, 369, 1491
LweI GCATC 3 cut(s) 134, 1117, 1417
MaeI CTAG 2 cut(s) 368, 927
MaeIII GTNAC 2 cut(s) 1270, 1427
MalI GATC 5 cut(s) 105, 236, 635, 681, 936
MboI GATC 5 cut(s) 103, 234, 633, 679, 934
MboII GAAGA 9 cut(s) 205, 281, 471, 531, 682, 727, 926, 1066, 1138
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 3 cut(s) 73, 549, 1479
MluCI AATT 7 cut(s) 397, 554, 727, 870, 1292, 1356, 1414
MlyI GAGTC 3 cut(s) 1002, 1069, 1229
MmeI TCCRAC 2 cut(s) 340, 1421
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 1388
MseI TTAA 6 cut(s) 344, 768, 942, 1065, 1413, 1418
MspA1I CMGCKG 1 cut(s) 140
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 4 cut(s) 392, 962, 1041, 1393
MspR9I CCNGG 3 cut(s) 850, 963, 1394
Mva1269I GAATGC 1 cut(s) 33
MvaI CCWGG 1 cut(s) 850
MwoI GCNNNNNNNGC 7 cut(s) 68, 146, 251, 354, 981, 1102, 1513
NciI CCSGG 2 cut(s) 963, 1394
NdeI CATATG 1 cut(s) 1138
NdeII GATC 5 cut(s) 103, 234, 633, 679, 934
NlaIII CATG 9 cut(s) 160, 245, 352, 748, 984, 1001, 1367, 1406, 1435
NlaIV GGNNCC 1 cut(s) 840
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 1427
NspI RCATGY 1 cut(s) 1435
NspV TTCGAA 2 cut(s) 267, 1008
PaeR7I CTCGAG 1 cut(s) 471
PaqCI CACCTGC 1 cut(s) 725
PctI GAATGC 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 1388
PfeI GAWTC 6 cut(s) 462, 522, 955, 1030, 1240, 1277
PflMI CCANNNNNTGG 1 cut(s) 1217
PfoI TCCNGGA 2 cut(s) 848, 1392
PkrI GCNGC 5 cut(s) 139, 356, 359, 1107, 1506
PleI GAGTC 3 cut(s) 1001, 1069, 1229
PpsI GAGTC 3 cut(s) 1001, 1069, 1229
PpuMI RGGWCCY 1 cut(s) 839
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 1 cut(s) 848
PspGI CCWGG 1 cut(s) 848
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 2 cut(s) 839, 974
PspPPI RGGWCCY 1 cut(s) 839
PstI CTGCAG 2 cut(s) 139, 359
PsuI RGATCY 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 6 cut(s) 344, 768, 942, 1065, 1413, 1418
SatI GCNGC 5 cut(s) 138, 355, 358, 1106, 1505
Sau3AI GATC 5 cut(s) 103, 234, 633, 679, 934
Sau96I GGNCC 2 cut(s) 839, 974
SchI GAGTC 3 cut(s) 1002, 1069, 1229
ScrFI CCNGG 3 cut(s) 850, 963, 1394
SduI GDGCHC 3 cut(s) 73, 549, 1479
SfaNI GCATC 3 cut(s) 134, 1117, 1417
SfcI CTRYAG 2 cut(s) 135, 355
Sfr274I CTCGAG 1 cut(s) 471
SfuI TTCGAA 2 cut(s) 267, 1008
SinI GGWCC 1 cut(s) 839
SlaI CTCGAG 1 cut(s) 471
SmlI CTYRAG 6 cut(s) 308, 471, 542, 548, 941, 988
SmoI CTYRAG 6 cut(s) 308, 471, 542, 548, 941, 988
Sse9I AATT 7 cut(s) 397, 554, 727, 870, 1292, 1356, 1414
SsiI CCGC 5 cut(s) 5, 186, 224, 1106, 1367
SspI AATATT 1 cut(s) 751
SspMI CTAG 2 cut(s) 368, 927
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 848, 961, 1392
TaaI ACNGT 3 cut(s) 571, 1302, 1425
TaqI TCGA 6 cut(s) 267, 472, 711, 1008, 1086, 1238
TasI AATT 7 cut(s) 397, 554, 727, 870, 1292, 1356, 1414
TauI GCSGC 1 cut(s) 1108
TfiI GAWTC 6 cut(s) 462, 522, 955, 1030, 1240, 1277
Tru1I TTAA 6 cut(s) 344, 768, 942, 1065, 1413, 1418
Tru9I TTAA 6 cut(s) 344, 768, 942, 1065, 1413, 1418
TscAI CASTG 3 cut(s) 574, 916, 1430
TseFI GTSAC 1 cut(s) 1427
TseI GCWGC 4 cut(s) 137, 354, 357, 1504
Tsp45I GTSAC 1 cut(s) 1427
TspDTI ATGAA 6 cut(s) 159, 206, 761, 849, 969, 1164
TspGWI ACGGA 2 cut(s) 377, 438
TspRI CASTG 3 cut(s) 574, 916, 1430
Van91I CCANNNNNTGG 1 cut(s) 1217
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 2 cut(s) 397, 1292
XceI RCATGY 1 cut(s) 1435
XhoI CTCGAG 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 1388
XspI CTAG 2 cut(s) 368, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.