Rh2BG007800

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006173
Physical Location & Seq
Reverse (-)
560169 .. 562800
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG007800.1

Sequence Viewer

Length: 1635 bp
ATGGCGGAGTCAGTTGGGATGCCGGAGACTGATATAACGGCGTTAACCGAAGCGCTCAAGTCTCAGCAACAGCTTCTGCAAATGCTTTATGCTGATCTTGATCAGGAAAGAGAAGCTTCATCTACTGCAGCTGATGAAGCTCTATCCATGATACTGCGTTTGCAAGGAGAGAAGTCTGCTGTGAAGATGGAGGCCAATCAATACAAGAGATTGGCAGAGGAAAAGATATGTCATGCCGAGGAAGCTCTAGCGATTTTCGAGGATCTTATTTATCAGAAGGAAATGGAAATTGCTTCTTTGGAGTTTCAGCTTCAGGCTTATAGGTACAAACTGTTGAGCTTGGGTGTCAGTACCGAATTAGGTACCACCGAAAATGTGTATCCGGAAAACTTGTTGCAGCAAAGAGGTGATGGGGAAGCGGGTGGTGTTAGTGGGGCAATTAGAAGAATCAACTCTCTGCCTCCACTTGAGCTCAAGGATTTCCTCAACAAGAAATGCCCTGTAGAAAGAGAGAGTGAAAGTCTACCGAAAGAAAAACCTATGATACCAAAGCCAGAATCTTGTATGAAAAAGGGAGACAAAAAGGTGGACAAGGAAGTTACTTTCCAGGGCATTGATGCAGAGAAGAAATCGAGAAATGCTTCCAATGGCAACCTTAATTCGATCTGGGAGCAGATTAAGAAGTTAGATGACCGAGTGAGAGAGATATCAGATTGTCATGATTCTGGTGGTAGAGGAAAATCTGGAATTTTGAAGGGTCCGGCTAGGACATGTTCATTACCTCCAATTCCGAAAGTAATCACAAGCACATCCCATGATACAGAAAGTGAAGATATTATTGCTACCTTTGAAAGAGTTAATCATAATGAGAATGTCCAGGAAACGGAGGCAATTGTTAGTACTACATGCTCGACTGGCGTCCATGATGTTTTTGAAGTTCCACAGAGCTATGAATGCAGTCCTAAAACTTTGGACAGTCCTAAATCTTTTGAAGGTGAGAAGAAAGAGTTTGATAAATTGACATTTGGTGTTGAGAGTTCTAAAGCTTTTGAAGTTGAGAAGAAAGAATTTATTGGCAAAGATGATTTGATATGTGATGAGATTAGTGAAGTGCATGTTCAAGATGAGACAGAGAGGGTTAAGAAAAGGTTTCATGGCAAGTTGCTCGAGAATAGAATATTGAGTCGAACGACCACTGTTGACTCAACTGCAAGGGAGAGAGTGGCTGCTGCGGTTACTGAGAATCAAGCCAGATTTCAACAGTTGTGGAGGAGGATAGAGCGGCTTGAGGGAGAGAGAAGTAGTGTAAGGAACGAAATAAGTCATGCAGGAGAAGAGGAGTTGAAGTTGTTTAGGGATATACGTGAGCAACTTAACTTAATTCGGTCTGAAATACATATTTGGAAGCCTAAGCAACCTCCTCCACAGGATGACGAGAACTTACACAGAAGTACTGTAAGCAGGAATGAAATAAGTCATGCAGGAGAAGAGGATTTGAAGTTGTTTAGGGATATACGTGAGCAACTTAACTTAATTTGGTCTGAAATACATACTTGGAAGCCTAAGCAACCTCCTCGACAGGATGACGAGCCCTTACACATTGTTCAAGAGGCAATGCTACACTTTTGGCTCTGA

Protein Analysis

544

Amino Acids

62.06

Weight (kDa)

5.25

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 14 - 104 1e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 523
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 4 cut(s) 5, 419, 1232, 1282
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 747, 1067
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 5 cut(s) 326, 352, 364, 901, 1453
AfeI AGCGCT 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 883
AflIII ACRYGT 1 cut(s) 770
AjnI CCWGG 2 cut(s) 606, 876
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 20, 66, 570, 1121
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 1166
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 4 cut(s) 128, 397, 1226, 1229
ApoI RAATTY 2 cut(s) 747, 1067
Asp700I GAANNNNTTC 1 cut(s) 640
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 758
AsuHPI GGTGA 2 cut(s) 419, 1007
AvaI CYCGRG 1 cut(s) 1166
AvaII GGWCC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 2 cut(s) 474, 1593
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 4 cut(s) 140, 409, 1213, 1216
BccI CCATC 2 cut(s) 181, 404
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 4 cut(s) 1147, 1181, 1556, 1590
BciT130I CCWGG 2 cut(s) 608, 878
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 4 cut(s) 20, 66, 570, 1121
BfaI CTAG 2 cut(s) 248, 765
BfmI CTRYAG 2 cut(s) 126, 501
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 5 cut(s) 129, 398, 1227, 1230, 1283
BlsI GCNGC 5 cut(s) 130, 399, 1228, 1231, 1284
BmcAI AGTACT 2 cut(s) 901, 1453
Bme1390I CCNGG 2 cut(s) 608, 878
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 2 cut(s) 364, 759
BmrFI CCNGG 2 cut(s) 608, 878
BmsI GCATC 2 cut(s) 9, 607
BoxI GACNNNNGTC 1 cut(s) 917
Bpu10I CCTNAGC 2 cut(s) 1410, 1563
BpuEI CTTGAG 4 cut(s) 41, 458, 488, 1307
BsaAI YACGTR 2 cut(s) 1364, 1517
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 2 cut(s) 237, 607
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 1331, 1361
Bsc4I CCNNNNNNNGG 1 cut(s) 883
Bse1I ACTGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 1620
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 2 cut(s) 608, 878
BseDI CCNNGG 2 cut(s) 237, 607
BseGI GGATG 4 cut(s) 24, 809, 1435, 1588
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 883
BseMI GCAATG 1 cut(s) 1620
BseMII CTCAG 2 cut(s) 77, 1230
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 4 cut(s) 1285, 1352, 1410, 1563
BseXI GCAGC 4 cut(s) 140, 409, 1213, 1216
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 1166
BsiSI CCGG 3 cut(s) 23, 383, 761
BslI CCNNNNNNNGG 1 cut(s) 883
BsmAI GTCTC 4 cut(s) 20, 66, 570, 1121
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 1166
Bsp1286I GDGCHC 2 cut(s) 474, 1593
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 4 cut(s) 94, 100, 262, 663
BspACI CCGC 4 cut(s) 5, 419, 1232, 1282
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 76, 1231
BspEI TCCGGA 1 cut(s) 382
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 2 cut(s) 364, 759
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 1282
BsrDI GCAATG 1 cut(s) 1620
BsrI ACTGG 1 cut(s) 919
BssECI CCNNGG 2 cut(s) 237, 607
BssMI GATC 4 cut(s) 94, 100, 262, 663
BssNI GRCGYC 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 608, 878
Bst4CI ACNGT 5 cut(s) 333, 977, 1198, 1263, 1456
Bst6I CTCTTC 2 cut(s) 1329, 1482
BstACI GRCGYC 1 cut(s) 918
BstBAI YACGTR 2 cut(s) 1364, 1517
BstDEI CTNAG 4 cut(s) 63, 1239, 1410, 1563
BstF5I GGATG 4 cut(s) 24, 809, 1435, 1588
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 4 cut(s) 97, 103, 265, 666
BstMAI GTCTC 4 cut(s) 20, 66, 570, 1121
BstMBI GATC 4 cut(s) 94, 100, 262, 663
BstMWI GCNNNNNNNGC 4 cut(s) 137, 242, 915, 954
BstNI CCWGG 2 cut(s) 608, 878
BstNSI RCATGY 3 cut(s) 774, 909, 1118
BstPAI GACNNNNGTC 1 cut(s) 917
BstSCI CCNGG 2 cut(s) 606, 876
BstSFI CTRYAG 2 cut(s) 126, 501
BstV1I GCAGC 4 cut(s) 140, 409, 1213, 1216
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 194
BtsCI GGATG 4 cut(s) 24, 809, 1435, 1588
BtsIMutI CAGTG 1 cut(s) 1194
CaiI CAGNNNCTG 1 cut(s) 76
CciI TCATGA 1 cut(s) 718
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 758
CseI GACGC 1 cut(s) 907
Csp6I GTAC 5 cut(s) 325, 351, 363, 900, 1452
CspCI CAANNNNNGTGG 2 cut(s) 1247, 1282
CviQI GTAC 5 cut(s) 325, 351, 363, 900, 1452
DdeI CTNAG 4 cut(s) 63, 1239, 1410, 1563
DpnI GATC 4 cut(s) 96, 102, 264, 665
DpnII GATC 4 cut(s) 94, 100, 262, 663
Eam1104I CTCTTC 2 cut(s) 1329, 1482
EarI CTCTTC 2 cut(s) 1329, 1482
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 2 cut(s) 474, 1593
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 758
Eco47III AGCGCT 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 1166
EcoICRI GAGCTC 1 cut(s) 472
EcoRII CCWGG 2 cut(s) 606, 876
EcoRV GATATC 1 cut(s) 708
EcoT38I GRGCYC 2 cut(s) 474, 1593
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FauI CCCGC 1 cut(s) 412
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 5 cut(s) 129, 398, 1227, 1230, 1283
FokI GGATG 4 cut(s) 31, 796, 1442, 1595
FriOI GRGCYC 2 cut(s) 474, 1593
Fsp4HI GCNGC 5 cut(s) 129, 398, 1227, 1230, 1283
FspBI CTAG 2 cut(s) 248, 765
GlaI GCGC 1 cut(s) 54
GluI GCNGC 5 cut(s) 129, 398, 1227, 1230, 1283
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 23, 383, 761
HgaI GACGC 1 cut(s) 907
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 918
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 2 cut(s) 45, 1201
HindII GTYRAC 2 cut(s) 45, 1201
HindIII AAGCTT 2 cut(s) 114, 1044
HinfI GANTC 7 cut(s) 8, 447, 557, 722, 1183, 1202, 1243
HpaI GTTAAC 1 cut(s) 45
HpaII CCGG 3 cut(s) 23, 383, 761
HphI GGTGA 2 cut(s) 419, 1007
Hpy166II GTNNAC 4 cut(s) 45, 524, 589, 1201
Hpy188I TCNGA 6 cut(s) 276, 712, 792, 1390, 1543, 1634
Hpy188III TCNNGA 9 cut(s) 98, 104, 383, 633, 719, 744, 1121, 1168, 1607
Hpy8I GTNNAC 4 cut(s) 45, 524, 589, 1201
HpyAV CCTTC 3 cut(s) 271, 748, 986
HpyCH4III ACNGT 5 cut(s) 333, 977, 1198, 1263, 1456
HpyCH4IV ACGT 2 cut(s) 1363, 1516
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 242, 915, 954
HpyF3I CTNAG 4 cut(s) 63, 1239, 1410, 1563
HpySE526I ACGT 2 cut(s) 1363, 1516
Hsp92I GRCGYC 1 cut(s) 918
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 382
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 4 cut(s) 94, 100, 262, 663
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 4 cut(s) 140, 409, 1213, 1216
LweI GCATC 2 cut(s) 9, 607
MaeI CTAG 2 cut(s) 248, 765
MaeII ACGT 2 cut(s) 1363, 1516
MaeIII GTNAC 2 cut(s) 598, 1234
MalI GATC 4 cut(s) 96, 102, 264, 665
MbiI CCGCTC 1 cut(s) 1282
MboI GATC 4 cut(s) 94, 100, 262, 663
MboII GAAGA 8 cut(s) 196, 456, 637, 842, 1012, 1072, 1346, 1499
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 474, 1593
MlyI GAGTC 3 cut(s) 17, 1192, 1196
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 9 cut(s) 44, 657, 678, 858, 1140, 1374, 1379, 1527, 1532
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 3 cut(s) 23, 383, 761
MspR9I CCNGG 2 cut(s) 608, 878
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 2 cut(s) 608, 878
MwoI GCNNNNNNNGC 4 cut(s) 137, 242, 915, 954
NdeII GATC 4 cut(s) 94, 100, 262, 663
NlaIV GGNNCC 2 cut(s) 364, 759
NmeAIII GCCGAG 1 cut(s) 262
NspI RCATGY 3 cut(s) 774, 909, 1118
PaeR7I CTCGAG 1 cut(s) 1166
PagI TCATGA 1 cut(s) 718
PciI ACATGT 1 cut(s) 770
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 4 cut(s) 447, 557, 722, 1243
PfoI TCCNGGA 1 cut(s) 876
PkrI GCNGC 5 cut(s) 130, 399, 1228, 1231, 1284
PleI GAGTC 3 cut(s) 16, 1191, 1196
PpsI GAGTC 3 cut(s) 16, 1191, 1196
Ppu21I YACGTR 2 cut(s) 1364, 1517
PscI ACATGT 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 917
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 606, 876
PspGI CCWGG 2 cut(s) 606, 876
PspN4I GGNNCC 2 cut(s) 364, 759
PspPI GGNCC 1 cut(s) 758
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 5 cut(s) 326, 352, 364, 901, 1453
RsaNI GTAC 5 cut(s) 325, 351, 363, 900, 1452
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 9 cut(s) 44, 657, 678, 858, 1140, 1374, 1379, 1527, 1532
SatI GCNGC 5 cut(s) 129, 398, 1227, 1230, 1283
Sau3AI GATC 4 cut(s) 94, 100, 262, 663
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 2 cut(s) 901, 1453
SchI GAGTC 3 cut(s) 17, 1192, 1196
ScrFI CCNGG 2 cut(s) 608, 878
SduI GDGCHC 2 cut(s) 474, 1593
SfaNI GCATC 2 cut(s) 9, 607
SfcI CTRYAG 2 cut(s) 126, 501
Sfr274I CTCGAG 1 cut(s) 1166
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 1166
SmlI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
SmoI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
SsiI CCGC 4 cut(s) 5, 419, 1232, 1282
SspI AATATT 1 cut(s) 1179
SspMI CTAG 2 cut(s) 248, 765
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 2 cut(s) 606, 876
TaaI ACNGT 5 cut(s) 333, 977, 1198, 1263, 1456
TaiI ACGT 2 cut(s) 1366, 1519
TaqI TCGA 7 cut(s) 258, 632, 662, 911, 1167, 1186, 1576
TaqII GACCGA 2 cut(s) 708, 1374
TatI WGTACW 2 cut(s) 899, 1451
TauI GCSGC 1 cut(s) 1285
TfiI GAWTC 4 cut(s) 447, 557, 722, 1243
Tru1I TTAA 9 cut(s) 44, 657, 678, 858, 1140, 1374, 1379, 1527, 1532
Tru9I TTAA 9 cut(s) 44, 657, 678, 858, 1140, 1374, 1379, 1527, 1532
TscAI CASTG 1 cut(s) 1201
TseI GCWGC 4 cut(s) 128, 397, 1226, 1229
TspDTI ATGAA 7 cut(s) 108, 150, 581, 765, 966, 1142, 1482
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 1 cut(s) 1201
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 2 cut(s) 747, 1067
XceI RCATGY 3 cut(s) 774, 909, 1118
XhoI CTCGAG 1 cut(s) 1166
XmiI GTMKAC 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 640
XspI CTAG 2 cut(s) 248, 765
ZrmI AGTACT 2 cut(s) 901, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.