MD15G1149000.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006173
Physical Location & Seq
Reverse (-)
11024855 .. 11027654
2800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1149000.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGGCGGAGGGTAGTGTGCCTTGTGGGATGCCTGAAACTGATATTACAACGCTAACCGAAGCGCTTAGAGCACAGCAGCAACTGCTGCAAAAGCTTTACTATGATCTGGATCAGGAAAGAGAAGCATCATCTACTGCAGCTGATGAAGCTCTATCCATGATATTGCGTTTGCAAGGAGAAAAGTCCGCTATGAAGATGGAAGCGAGTCAATACAAGAGATTGGCGGAGGAAAAGATTTGTCATTCCGAGGAAGCTCTTGCCATTTTCGAAGATCTCATTTATCAGAAAGAAATGGAAATTGCTTCGCTTGAGTTCCAACTTCAGGCTTATAGGCACAAGCTTTTAAGCATGGGCTGCAGCACGGAGTTAGGCGCTGGTGAAAACGTATATCCGGAGAATCTGTTGTTTCAAAGGAATGATTTTGGAAGTGGGGAAGCAGGTGTTAATGGGACGATTAGAAGAATCAACTCTCGATGCGATTTTGGCAGTGGGGAAACAGGTGTTAGCGGGGCTATTAGAAGAATCAACTCTCTACCTCCAATTGAGCTCAAGGAATTGCTCAAACAGAAAAGCACTATGGAAAGAGAAAGAGATAAAGAAAGAGAACGAGATAGAGACAGACCAATGATCCCCAAACCCGAACCTGCTACAAAAATACAGGAGAAGAAGGTGGAGCAGGAAGTCAATGTCCAGAGCCTAGATGTAGAGAAGAAATTTCCAAATGATGCAGGCGGGAACATGAATACAATATGGGAGCAGATTAAGAAGTTAGATGCGCGAGTGAAAGAGATATCCGATTATAAAGATTATGGTAGGGGAAAATCTGTACTCTTGAAGGGTCCTCCATCTAGGACTTGTTCATTGCCTCCAATTCCGAAGATATGCATAAGCCCAAAAAATGAACTCGACAGTGAAGAAATTATTGCTAGTTTGGATCAACTTAAGCAGAGTGAGAATCTACAGGGAAGGGAGCTGACTGTTAGCCCTTCGTGCTCCTCAAGCGTCTATGATGTCTTCGAAGTCCCACAAAGTTATGAAAACAGTAAAGCTGGTGAAGGAGAGAAGAAAGAACGTAGTGCATTGACTGTGAAACTTGACAAGAGGCTTGGTAAGCCGGATTTGGTCATGGGTGAGACGACAGAAGCATATGTTAGAGATGAAACGGATAGGGTAAAGAAAATGTTGCATACCAAGAAGCAGGAGAACAAAATACCTAAACCAAGAGATGTGATAAGTAGTGCTGACTCGAATGTGAGGGAGAAAGCGGTGAGTGGTGGGGTTACTGAATCTCAGCAAGCGAAATTTCAACAGTTGTGGAGGAGAATAGAGCGGCTCGAGGGAGAAAGGAATAATATAAGGCAAGAAATTAGTCATGCGGGAGAAGAGGAGTTGAAGCTGTTCCAGGAGATACACGAGCATCTTAATTTAATACAGTCCGAAATGCGTAGTTGGAAGCCTAAGAAACCCCGTCCCCAGGATGATGAGCCCTTGCACAATGTTATGGAGGCAATGCTGCACTTTTGGCTTTGA

Protein Analysis

510

Amino Acids

57.99

Weight (kDa)

5.63

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 17 - 107 1.1e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AarI CACCTGC 1 cut(s) 428
Acc36I ACCTGC 2 cut(s) 428, 652
AccBSI CCGCTC 1 cut(s) 1330
AccII CGCG 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 8 cut(s) 5, 186, 224, 507, 732, 1265, 1330, 1376
AclWI GGATC 3 cut(s) 117, 622, 942
AcsI RAATTY 2 cut(s) 713, 1301
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 1 cut(s) 828
AfeI AGCGCT 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 322, 1474
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 4 cut(s) 410, 835, 1307, 1393
AjnI CCWGG 2 cut(s) 1401, 1473
AluBI AGCT 9 cut(s) 94, 140, 149, 254, 340, 547, 973, 1049, 1396
AluI AGCT 9 cut(s) 94, 140, 149, 254, 340, 547, 973, 1049, 1396
Alw21I GWGCWC 3 cut(s) 73, 549, 995
Alw26I GTCTC 2 cut(s) 609, 1127
AlwI GGATC 3 cut(s) 117, 622, 942
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 1334
Aor13HI TCCGGA 1 cut(s) 391
Aor51HI AGCGCT 1 cut(s) 63
ApeKI GCWGC 6 cut(s) 76, 85, 137, 354, 357, 1513
ApoI RAATTY 2 cut(s) 713, 1301
Asp700I GAANNNNTTC 1 cut(s) 1397
AspLEI GCGC 3 cut(s) 64, 374, 778
AspS9I GGNCC 1 cut(s) 839
AsuHPI GGTGA 4 cut(s) 389, 1064, 1142, 1279
AsuII TTCGAA 2 cut(s) 267, 1017
AvaI CYCGRG 1 cut(s) 1334
AvaII GGWCC 1 cut(s) 839
BanII GRGCYC 2 cut(s) 549, 1488
BauI CACGAG 1 cut(s) 1412
BbsI GAAGAC 1 cut(s) 1006
Bbv12I GWGCWC 3 cut(s) 73, 549, 995
BbvI GCAGC 6 cut(s) 72, 88, 149, 341, 369, 1500
BccI CCATC 2 cut(s) 190, 853
BciT130I CCWGG 2 cut(s) 1403, 1475
BcoDI GTCTC 2 cut(s) 609, 1127
BfaI CTAG 3 cut(s) 698, 849, 927
BfmI CTRYAG 3 cut(s) 135, 355, 959
BfoI RGCGCY 2 cut(s) 65, 375
BfrI CTTAAG 1 cut(s) 941
BfuAI ACCTGC 2 cut(s) 428, 652
BglII AGATCT 1 cut(s) 271
BisI GCNGC 7 cut(s) 77, 86, 138, 355, 358, 1331, 1514
BlsI GCNGC 7 cut(s) 78, 87, 139, 356, 359, 1332, 1515
Bme1390I CCNGG 2 cut(s) 1403, 1475
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 1 cut(s) 1334
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 2 cut(s) 1403, 1475
BmsI GCATC 6 cut(s) 18, 134, 464, 715, 763, 1426
BpiI GAAGAC 1 cut(s) 1006
Bpu14I TTCGAA 2 cut(s) 267, 1017
BpuEI CTTGAG 3 cut(s) 329, 533, 982
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 246, 1473
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 1474
Bse3DI GCAATG 2 cut(s) 860, 1515
Bse8I GATNNNNATC 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 2 cut(s) 1403, 1475
BseDI CCNNGG 2 cut(s) 246, 1473
BseGI GGATG 2 cut(s) 33, 1483
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 322, 1474
BseMI GCAATG 2 cut(s) 860, 1515
BseMII CTCAG 1 cut(s) 1304
BseRI GAGGAG 3 cut(s) 985, 1333, 1400
BseXI GCAGC 6 cut(s) 72, 88, 149, 341, 369, 1500
BsgI GTGCAG 1 cut(s) 1499
Bsh1236I CGCG 1 cut(s) 778
BsiHKAI GWGCWC 3 cut(s) 73, 549, 995
BsiHKCI CYCGRG 1 cut(s) 1334
BsiSI CCGG 2 cut(s) 392, 1115
BslFI GGGAC 3 cut(s) 463, 1007, 1455
BslI CCNNNNNNNGG 2 cut(s) 322, 1474
BsmAI GTCTC 2 cut(s) 609, 1127
BsmBI CGTCTC 1 cut(s) 1127
BsmFI GGGAC 3 cut(s) 463, 1007, 1455
BsoBI CYCGRG 1 cut(s) 1334
Bsp119I TTCGAA 2 cut(s) 267, 1017
Bsp1286I GDGCHC 4 cut(s) 73, 549, 995, 1488
Bsp13I TCCGGA 1 cut(s) 391
Bsp143I GATC 5 cut(s) 103, 109, 271, 627, 934
BspACI CCGC 8 cut(s) 5, 186, 224, 507, 732, 1265, 1330, 1376
BspCNI CTCAG 1 cut(s) 1303
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 1 cut(s) 840
BspMAI CTGCAG 2 cut(s) 139, 359
BspMI ACCTGC 2 cut(s) 428, 652
BspPI GGATC 3 cut(s) 117, 622, 942
BspT104I TTCGAA 2 cut(s) 267, 1017
BspTI CTTAAG 1 cut(s) 941
BsrBI CCGCTC 1 cut(s) 1330
BsrDI GCAATG 2 cut(s) 860, 1515
BssECI CCNNGG 2 cut(s) 246, 1473
BssMI GATC 5 cut(s) 103, 109, 271, 627, 934
BssSI CACGAG 1 cut(s) 1412
Bst2BI CACGAG 1 cut(s) 1412
Bst2UI CCWGG 2 cut(s) 1403, 1475
Bst4CI ACNGT 6 cut(s) 911, 979, 1043, 1087, 1311, 1434
Bst6I CTCTTC 1 cut(s) 1377
BstAFI CTTAAG 1 cut(s) 941
BstAPI GCANNNNNTGC 3 cut(s) 82, 85, 354
BstBI TTCGAA 2 cut(s) 267, 1017
BstC8I GCNNGC 2 cut(s) 730, 1296
BstDEI CTNAG 3 cut(s) 65, 1290, 1458
BstF5I GGATG 2 cut(s) 33, 1483
BstFNI CGCG 1 cut(s) 778
BstH2I RGCGCY 2 cut(s) 65, 375
BstHHI GCGC 3 cut(s) 64, 374, 778
BstKTI GATC 5 cut(s) 106, 112, 274, 630, 937
BstMAI GTCTC 2 cut(s) 609, 1127
BstMBI GATC 5 cut(s) 103, 109, 271, 627, 934
BstNI CCWGG 2 cut(s) 1403, 1475
BstSCI CCNGG 2 cut(s) 1401, 1473
BstSFI CTRYAG 3 cut(s) 135, 355, 959
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 6 cut(s) 72, 88, 149, 341, 369, 1500
BstV2I GAAGAC 1 cut(s) 1006
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BtsCI GGATG 2 cut(s) 33, 1483
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 2 cut(s) 493, 916
BveI ACCTGC 2 cut(s) 428, 652
Cac8I GCNNGC 2 cut(s) 730, 1296
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 3 cut(s) 64, 374, 778
Cfr13I GGNCC 1 cut(s) 839
CseI GACGC 1 cut(s) 991
Csp6I GTAC 1 cut(s) 827
CspCI CAANNNNNGTGG 2 cut(s) 1295, 1330
CviAII CATG 5 cut(s) 157, 349, 739, 1126, 1373
CviQI GTAC 1 cut(s) 827
DdeI CTNAG 3 cut(s) 65, 1290, 1458
DpnI GATC 5 cut(s) 105, 111, 273, 629, 936
DpnII GATC 5 cut(s) 103, 109, 271, 627, 934
Eam1104I CTCTTC 1 cut(s) 1377
EarI CTCTTC 1 cut(s) 1377
EciI GGCGGA 2 cut(s) 20, 239
Ecl136II GAGCTC 1 cut(s) 547
Eco24I GRGCYC 2 cut(s) 549, 1488
Eco32I GATATC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 839
Eco47III AGCGCT 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 1334
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 1401, 1473
EcoRV GATATC 1 cut(s) 792
EcoT22I ATGCAT 1 cut(s) 887
EcoT38I GRGCYC 2 cut(s) 549, 1488
Esp3I CGTCTC 1 cut(s) 1127
FaeI CATG 5 cut(s) 160, 352, 742, 1129, 1376
FaqI GGGAC 3 cut(s) 463, 1007, 1455
FatI CATG 5 cut(s) 156, 348, 738, 1125, 1372
FauI CCCGC 3 cut(s) 500, 725, 1369
FauNDI CATATG 1 cut(s) 1147
Fnu4HI GCNGC 7 cut(s) 77, 86, 138, 355, 358, 1331, 1514
FokI GGATG 2 cut(s) 40, 1490
FriOI GRGCYC 2 cut(s) 549, 1488
Fsp4HI GCNGC 7 cut(s) 77, 86, 138, 355, 358, 1331, 1514
FspBI CTAG 3 cut(s) 698, 849, 927
GlaI GCGC 3 cut(s) 63, 373, 777
GluI GCNGC 7 cut(s) 77, 86, 138, 355, 358, 1331, 1514
HaeII RGCGCY 2 cut(s) 65, 375
HapII CCGG 2 cut(s) 392, 1115
HgaI GACGC 1 cut(s) 991
HhaI GCGC 3 cut(s) 64, 374, 778
Hin1II CATG 5 cut(s) 160, 352, 742, 1129, 1376
Hin6I GCGC 3 cut(s) 62, 372, 776
HinP1I GCGC 3 cut(s) 62, 372, 776
HindIII AAGCTT 2 cut(s) 92, 338
HinfI GANTC 7 cut(s) 205, 397, 462, 522, 955, 1244, 1286
HpaII CCGG 2 cut(s) 392, 1115
HphI GGTGA 4 cut(s) 389, 1064, 1142, 1279
Hpy188I TCNGA 5 cut(s) 247, 285, 796, 876, 1438
Hpy188III TCNNGA 6 cut(s) 107, 113, 392, 471, 691, 832
HpyAV CCTTC 5 cut(s) 661, 829, 960, 996, 1049
HpyCH4III ACNGT 6 cut(s) 911, 979, 1043, 1087, 1311, 1434
HpyCH4IV ACGT 2 cut(s) 384, 1072
HpyF3I CTNAG 3 cut(s) 65, 1290, 1458
HpySE526I ACGT 2 cut(s) 384, 1072
Hsp92II CATG 5 cut(s) 160, 352, 742, 1129, 1376
HspAI GCGC 3 cut(s) 62, 372, 776
Kpn2I TCCGGA 1 cut(s) 391
Kzo9I GATC 5 cut(s) 103, 109, 271, 627, 934
LmnI GCTCC 4 cut(s) 673, 754, 970, 998
Lsp1109I GCAGC 6 cut(s) 72, 88, 149, 341, 369, 1500
LweI GCATC 6 cut(s) 18, 134, 464, 715, 763, 1426
MaeI CTAG 3 cut(s) 698, 849, 927
MaeII ACGT 2 cut(s) 384, 1072
MaeIII GTNAC 1 cut(s) 1279
MalI GATC 5 cut(s) 105, 111, 273, 629, 936
MbiI CCGCTC 1 cut(s) 1330
MboI GATC 5 cut(s) 103, 109, 271, 627, 934
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 4 cut(s) 73, 549, 995, 1488
MluCI AATT 9 cut(s) 297, 540, 554, 713, 870, 918, 1301, 1365, 1423
MlyI GAGTC 2 cut(s) 214, 1238
MmeI TCCRAC 2 cut(s) 340, 1430
Mph1103I ATGCAT 1 cut(s) 887
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 1397
MseI TTAA 6 cut(s) 344, 444, 762, 942, 1422, 1427
MspA1I CMGCKG 1 cut(s) 140
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 2 cut(s) 392, 1115
MspR9I CCNGG 2 cut(s) 1403, 1475
MunI CAATTG 1 cut(s) 540
MvaI CCWGG 2 cut(s) 1403, 1475
MvnI CGCG 1 cut(s) 778
NdeI CATATG 1 cut(s) 1147
NdeII GATC 5 cut(s) 103, 109, 271, 627, 934
NlaIII CATG 5 cut(s) 160, 352, 742, 1129, 1376
NlaIV GGNNCC 1 cut(s) 840
NsiI ATGCAT 1 cut(s) 887
NspV TTCGAA 2 cut(s) 267, 1017
PaeR7I CTCGAG 1 cut(s) 1334
PaqCI CACCTGC 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 1397
PfeI GAWTC 5 cut(s) 397, 462, 522, 955, 1286
PfoI TCCNGGA 1 cut(s) 1401
PkrI GCNGC 7 cut(s) 78, 87, 139, 356, 359, 1332, 1515
PleI GAGTC 2 cut(s) 213, 1238
PpsI GAGTC 2 cut(s) 213, 1238
PpuMI RGGWCCY 1 cut(s) 839
PsiI TTATAA 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 2 cut(s) 1401, 1473
PspGI CCWGG 2 cut(s) 1401, 1473
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 1 cut(s) 839
PspPPI RGGWCCY 1 cut(s) 839
PspXI VCTCGAGB 1 cut(s) 1334
PstI CTGCAG 2 cut(s) 139, 359
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 1 cut(s) 828
RsaNI GTAC 1 cut(s) 827
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 6 cut(s) 344, 444, 762, 942, 1422, 1427
SatI GCNGC 7 cut(s) 77, 86, 138, 355, 358, 1331, 1514
Sau3AI GATC 5 cut(s) 103, 109, 271, 627, 934
Sau96I GGNCC 1 cut(s) 839
SchI GAGTC 2 cut(s) 214, 1238
ScrFI CCNGG 2 cut(s) 1403, 1475
SduI GDGCHC 4 cut(s) 73, 549, 995, 1488
SfaNI GCATC 6 cut(s) 18, 134, 464, 715, 763, 1426
SfcI CTRYAG 3 cut(s) 135, 355, 959
Sfr274I CTCGAG 1 cut(s) 1334
SfuI TTCGAA 2 cut(s) 267, 1017
SinI GGWCC 1 cut(s) 839
SlaI CTCGAG 1 cut(s) 1334
SmlI CTYRAG 5 cut(s) 308, 548, 941, 997, 1334
SmoI CTYRAG 5 cut(s) 308, 548, 941, 997, 1334
Sse9I AATT 9 cut(s) 297, 540, 554, 713, 870, 918, 1301, 1365, 1423
SsiI CCGC 8 cut(s) 5, 186, 224, 507, 732, 1265, 1330, 1376
SspMI CTAG 3 cut(s) 698, 849, 927
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 2 cut(s) 1401, 1473
TaaI ACNGT 6 cut(s) 911, 979, 1043, 1087, 1311, 1434
TaiI ACGT 2 cut(s) 387, 1075
TaqI TCGA 6 cut(s) 267, 472, 906, 1017, 1247, 1335
TasI AATT 9 cut(s) 297, 540, 554, 713, 870, 918, 1301, 1365, 1423
TatI WGTACW 1 cut(s) 826
TauI GCSGC 1 cut(s) 1333
TfiI GAWTC 5 cut(s) 397, 462, 522, 955, 1286
Tru1I TTAA 6 cut(s) 344, 444, 762, 942, 1422, 1427
Tru9I TTAA 6 cut(s) 344, 444, 762, 942, 1422, 1427
TscAI CASTG 2 cut(s) 493, 916
TseI GCWGC 6 cut(s) 76, 85, 137, 354, 357, 1513
TspDTI ATGAA 7 cut(s) 159, 206, 755, 849, 915, 1050, 1173
TspGWI ACGGA 2 cut(s) 377, 1178
TspRI CASTG 2 cut(s) 493, 916
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 839
XapI RAATTY 2 cut(s) 713, 1301
XhoI CTCGAG 1 cut(s) 1334
XmnI GAANNNNTTC 1 cut(s) 1397
XspI CTAG 3 cut(s) 698, 849, 927
Zsp2I ATGCAT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.