Rorug01G0458500

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006173
Physical Location & Seq
Reverse (-)
55261263 .. 55263199
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0458500.1

Sequence Viewer

Length: 330 bp
ATGGACCGATGTCATCGGAAGCGACCCAAAATCACTACTTCTGATCAGTCTGAAGAGGTTGTTAGCAGTGAATGGGAGTTCATTCATATGAGTGAGCAAGAAGAAGATCTCATCTATAGAATGTATAGACTTGTCGGAGAAAGGTGGGATTTAATAGCTGGTCGGGTTCCGGGTCGAACAGCAGTAGAATTGGAGAGGTTTTGGATAATGAGACATTGTGAGGTGTTTGCTGAGAAACGAAGGAAGCATAAGAAAGAAAACTCAAAAATTGTTCCGCGTAGAACTTTCAATGGATTTGTTCTTAGCAACTGTTTCGTAAAAAGCTTGTAA

Protein Analysis

109

Amino Acids

13.2

Weight (kDa)

9.36

Isoelectric Point (pI)

62.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 30 - 70 1.3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 277
AciI CCGC 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 72
AgsI TTSAA 1 cut(s) 289
AluBI AGCT 2 cut(s) 158, 324
AluI AGCT 2 cut(s) 158, 324
Alw26I GTCTC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 4
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 115
BglII AGATCT 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 171
BoxI GACNNNNGTC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 277
BsiSI CCGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 205
Bsp143I GATC 2 cut(s) 43, 106
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 168
BssMI GATC 2 cut(s) 43, 106
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 48
BstDEI CTNAG 2 cut(s) 231, 302
BstFNI CGCG 1 cut(s) 277
BstKTI GATC 2 cut(s) 46, 109
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 43, 106
BstPAI GACNNNNGTC 1 cut(s) 9
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 115
BstUI CGCG 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 4
CviJI RGCY 2 cut(s) 158, 324
CviKI_1 RGCY 2 cut(s) 158, 324
DdeI CTNAG 2 cut(s) 231, 302
DpnI GATC 2 cut(s) 45, 108
DpnII GATC 2 cut(s) 43, 106
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 72
FaiI YATR 5 cut(s) 87, 89, 117, 126, 249
FauNDI CATATG 1 cut(s) 87
FbaI TGATCA 1 cut(s) 43
HapII CCGG 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 322
HpaII CCGG 1 cut(s) 170
Hpy188I TCNGA 4 cut(s) 18, 43, 52, 137
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 311
HpyF3I CTNAG 2 cut(s) 231, 302
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 2 cut(s) 43, 106
LpnPI CCDG 2 cut(s) 144, 183
MalI GATC 2 cut(s) 45, 108
MboI GATC 2 cut(s) 43, 106
MboII GAAGA 3 cut(s) 65, 113, 116
MflI RGATCY 1 cut(s) 106
MluCI AATT 2 cut(s) 188, 267
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 3 cut(s) 49, 189, 214
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 2 cut(s) 86, 90
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 277
NciI CCSGG 1 cut(s) 171
NdeI CATATG 1 cut(s) 87
NdeII GATC 2 cut(s) 43, 106
NlaIV GGNNCC 1 cut(s) 168
PshAI GACNNNNGTC 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 106
RseI CAYNNNNRTG 2 cut(s) 86, 90
SaqAI TTAA 1 cut(s) 152
Sau3AI GATC 2 cut(s) 43, 106
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 171
SetI ASST 6 cut(s) 60, 146, 160, 200, 225, 326
SfcI CTRYAG 1 cut(s) 115
SgeI CNNG 7 cut(s) 110, 143, 171, 176, 182, 183, 288
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 86, 90
Sse9I AATT 2 cut(s) 188, 267
SsiI CCGC 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 1 cut(s) 175
TaqII GACCGA 1 cut(s) 21
TasI AATT 2 cut(s) 188, 267
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TscAI CASTG 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 70, 74
TspRI CASTG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 4
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.