RLG00000015670

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006173
Physical Location & Seq
Reverse (-)
531025 .. 532754
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015670

Sequence Viewer

Length: 1548 bp
ATGGCGGAGTCAGTTGGGATGCCTGAGACTGATATAACAGCGTTAACCGAAGCGCTCAAGTCTCAGCAACAGCTGCTGCAAATGCTTTATGCTGATCTTGATCAGGAAAGAGAAGCTTCATCTACTGCAGCTGATGAAGCTCTATCCATGATACTGCGTTTGCAAGGTGAGAAGTCTGCTGTGAAGATGGAGGCCAATCAATACAAGAGATTGGCAGAGGAAAAGATATGTCATGCTGAGGAAGCTCTAGCGATTTTCGAGGATCTTATTTATCAGAAGGAAATGGAAATTGCTTCTTTGGAGTTTCAGCTTCAGGCTTATAGGTACAAACTGTTGAGCTTGGGTGTCAGTACTGAATTAGGTACCACCGAAAATGTGTATCCGGAAAACTTGTTGCAGCAAAGAGGTGATGGGGAAGCGAGTGGTGTTAGTGGGGCAATTAGAAGAATCAACTCTCTGCCTCCACTTGAGCTCAAGGATTTCCTCAACAAGAAATGCCCTGTAGAAAGAGAGAGTGAAAGTATACCGAAAGAAAAACCTATGATACCAAAGCCAGAATCTTGTATGAAAAAAGGAGACAAAAATGTGGACAAGGAAGTTACTTTCCAGGGCATTGATGCGGAGAAGAAATCCAGAAATGCTTCCAATGGCAACCTTAATTCGATCTGGGAGCAGATTAAGAAGTTAGACGACCGAGTGAGAGAGATATCAGATTGTAAAGATTCTGGTGGTAGAGGAAAATCTGGAATTTTGAAGGGTCCGGCTAGGACATGTTCATTACCTCCAATTCCAAAAGAAACGGAGGCAATTGTTAGTACTACATGCTCAACTGGCGTCCATGATGTTTTTGAAGTTCCACAGAGCTATGAATGCAGTCCTAAAACTTTGGACAGTCCTAAATCTTTTGAAGGTGAGAAGAAAGAGTTTGATAAATTGACATTTGATGTTGAGAGTTCTAAAGCTTTTGAAGGTGAGAAGAAAGAATTTATTGGCAAAGATGATTTGCTATGTGATGAGATCAGTGAACTGCATGTTCAAGATGAGACAGAGAGGGTTAAGAAAAGGTTTCATGGCAAGTTGCTCGAGAATAGAATATTGAGTCGAACGACCACTGTTGACTCAACTGCAAGGGAGAGAGTGGCTGCTGCGGTTACTGAGAATCAAGCCAGATTTCAACAGTTGTGGAGGAGGATAGAGCGGCTTGAGGGAGAGAGAAGTAGTGTAAGGAACGAAATAAGTCATGCAGGAGAAGAGGAGTTGAAGTTGTTTAGGGATATACGTGAGCAACTCAACTTAATTCGGTCTGAAATACATATTTGGAAGCCTAAGCAACCTCCTCCACAGGATGACGAGAACTTACACAGAAGTACTGTAAGGAATGAAATAAGTCATGCAGGAGAAGAGGATTTGAAGTTGTTTAGGGATATACGTGAGCAACTTAACTTAATTTCTTCTGAAATACATACTTGGAAGCCTAAGCAACCTCCTCGACAGGACGACGAGCCCTTACACATTGTTCAAGAGGCAATGCTACACTTTTGGCTCTGA

Protein Analysis

516

Amino Acids

58.74

Weight (kDa)

5.29

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 14 - 104 9.4e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 1198
AccI GTMKAC 1 cut(s) 523
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 4 cut(s) 5, 620, 1148, 1198
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 747, 983
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 5 cut(s) 326, 352, 364, 817, 1369
AfeI AGCGCT 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 770
AgsI TTSAA 9 cut(s) 754, 851, 908, 968, 1037, 1175, 1261, 1411, 1520
AjnI CCWGG 1 cut(s) 606
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 20, 66, 570, 1037
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 1082
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 6 cut(s) 73, 76, 128, 397, 1142, 1145
ApoI RAATTY 2 cut(s) 747, 983
Asp700I GAANNNNTTC 1 cut(s) 640
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 758
AsuHPI GGTGA 4 cut(s) 179, 419, 923, 983
AvaI CYCGRG 1 cut(s) 1082
AvaII GGWCC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 2 cut(s) 474, 1506
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 6 cut(s) 60, 63, 140, 409, 1129, 1132
BccI CCATC 2 cut(s) 181, 404
BcgI CGANNNNNNTGC 4 cut(s) 1063, 1097, 1469, 1503
BciT130I CCWGG 1 cut(s) 608
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 4 cut(s) 20, 66, 570, 1037
BfaI CTAG 2 cut(s) 248, 765
BfmI CTRYAG 2 cut(s) 126, 501
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 7 cut(s) 74, 77, 129, 398, 1143, 1146, 1199
BlsI GCNGC 7 cut(s) 75, 78, 130, 399, 1144, 1147, 1200
BmcAI AGTACT 3 cut(s) 352, 817, 1369
Bme1390I CCNGG 1 cut(s) 608
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 1082
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 2 cut(s) 364, 759
BmrFI CCNGG 1 cut(s) 608
BmsI GCATC 2 cut(s) 9, 607
Bpu10I CCTNAGC 3 cut(s) 237, 1326, 1476
BpuEI CTTGAG 4 cut(s) 41, 458, 488, 1223
BsaAI YACGTR 2 cut(s) 1280, 1430
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 834
BsaJI CCNNGG 1 cut(s) 607
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 1247, 1277
Bse1I ACTGG 1 cut(s) 835
Bse3DI GCAATG 1 cut(s) 1533
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 2 cut(s) 24, 1351
BseJI GATNNNNATC 1 cut(s) 99
BseMI GCAATG 1 cut(s) 1533
BseMII CTCAG 4 cut(s) 15, 77, 228, 1146
BseNI ACTGG 1 cut(s) 835
BseRI GAGGAG 4 cut(s) 1201, 1268, 1326, 1476
BseXI GCAGC 6 cut(s) 60, 63, 140, 409, 1129, 1132
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 1082
BsiSI CCGG 2 cut(s) 383, 761
BsmAI GTCTC 4 cut(s) 20, 66, 570, 1037
BsmI GAATGC 1 cut(s) 875
BsnI GGCC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 1082
Bsp1286I GDGCHC 2 cut(s) 474, 1506
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 5 cut(s) 94, 100, 262, 663, 1017
BspACI CCGC 4 cut(s) 5, 620, 1148, 1198
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 4 cut(s) 16, 76, 229, 1147
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 364, 759
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 1198
BsrDI GCAATG 1 cut(s) 1533
BsrI ACTGG 1 cut(s) 835
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 5 cut(s) 94, 100, 262, 663, 1017
BssNAI GTATAC 1 cut(s) 524
BssNI GRCGYC 1 cut(s) 834
Bst1107I GTATAC 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 5 cut(s) 333, 893, 1114, 1179, 1372
Bst6I CTCTTC 2 cut(s) 1245, 1395
BstACI GRCGYC 1 cut(s) 834
BstAPI GCANNNNNTGC 1 cut(s) 73
BstBAI YACGTR 2 cut(s) 1280, 1430
BstDEI CTNAG 6 cut(s) 24, 63, 237, 1155, 1326, 1476
BstF5I GGATG 2 cut(s) 24, 1351
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 5 cut(s) 97, 103, 265, 666, 1020
BstMAI GTCTC 4 cut(s) 20, 66, 570, 1037
BstMBI GATC 5 cut(s) 94, 100, 262, 663, 1017
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 831, 870
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 3 cut(s) 774, 825, 1034
BstSCI CCNGG 1 cut(s) 606
BstSFI CTRYAG 2 cut(s) 126, 501
BstV1I GCAGC 6 cut(s) 60, 63, 140, 409, 1129, 1132
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BstZ17I GTATAC 1 cut(s) 524
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 194
BtsCI GGATG 2 cut(s) 24, 1351
BtsIMutI CAGTG 2 cut(s) 1027, 1110
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 758
CseI GACGC 1 cut(s) 823
Csp6I GTAC 5 cut(s) 325, 351, 363, 816, 1368
CspCI CAANNNNNGTGG 2 cut(s) 1163, 1198
CviAII CATG 9 cut(s) 148, 233, 771, 822, 839, 1031, 1070, 1241, 1391
CviQI GTAC 5 cut(s) 325, 351, 363, 816, 1368
DdeI CTNAG 6 cut(s) 24, 63, 237, 1155, 1326, 1476
DpnI GATC 5 cut(s) 96, 102, 264, 665, 1019
DpnII GATC 5 cut(s) 94, 100, 262, 663, 1017
Eam1104I CTCTTC 2 cut(s) 1245, 1395
EarI CTCTTC 2 cut(s) 1245, 1395
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 2 cut(s) 474, 1506
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 758
Eco47III AGCGCT 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 1082
EcoICRI GAGCTC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 708
EcoT38I GRGCYC 2 cut(s) 474, 1506
FaeI CATG 9 cut(s) 151, 236, 774, 825, 842, 1034, 1073, 1244, 1394
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FatI CATG 9 cut(s) 147, 232, 770, 821, 838, 1030, 1069, 1240, 1390
FbaI TGATCA 1 cut(s) 100
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1143, 1146, 1199
FokI GGATG 2 cut(s) 31, 1358
FriOI GRGCYC 2 cut(s) 474, 1506
Fsp4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1143, 1146, 1199
FspBI CTAG 2 cut(s) 248, 765
GlaI GCGC 1 cut(s) 54
GluI GCNGC 7 cut(s) 74, 77, 129, 398, 1143, 1146, 1199
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 383, 761
HgaI GACGC 1 cut(s) 823
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 9 cut(s) 151, 236, 774, 825, 842, 1034, 1073, 1244, 1394
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 2 cut(s) 45, 1117
HindII GTYRAC 2 cut(s) 45, 1117
HindIII AAGCTT 2 cut(s) 114, 960
HinfI GANTC 7 cut(s) 8, 447, 557, 722, 1099, 1118, 1159
HpaI GTTAAC 1 cut(s) 45
HpaII CCGG 2 cut(s) 383, 761
HphI GGTGA 4 cut(s) 179, 419, 923, 983
Hpy166II GTNNAC 5 cut(s) 45, 524, 589, 1025, 1117
Hpy188I TCNGA 5 cut(s) 276, 712, 1306, 1456, 1547
Hpy188III TCNNGA 8 cut(s) 98, 104, 383, 633, 744, 1037, 1084, 1520
Hpy8I GTNNAC 5 cut(s) 45, 524, 589, 1025, 1117
Hpy99I CGWCG 1 cut(s) 1502
HpyAV CCTTC 4 cut(s) 271, 748, 902, 962
HpyCH4III ACNGT 5 cut(s) 333, 893, 1114, 1179, 1372
HpyCH4IV ACGT 2 cut(s) 1279, 1429
HpyCH4V TGCA 9 cut(s) 79, 128, 163, 397, 873, 1030, 1127, 1244, 1394
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 831, 870
HpyF3I CTNAG 6 cut(s) 24, 63, 237, 1155, 1326, 1476
HpySE526I ACGT 2 cut(s) 1279, 1429
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 9 cut(s) 151, 236, 774, 825, 842, 1034, 1073, 1244, 1394
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 382
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 100
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 5 cut(s) 94, 100, 262, 663, 1017
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 6 cut(s) 60, 63, 140, 409, 1129, 1132
LweI GCATC 2 cut(s) 9, 607
MaeI CTAG 2 cut(s) 248, 765
MaeII ACGT 2 cut(s) 1279, 1429
MaeIII GTNAC 2 cut(s) 598, 1150
MalI GATC 5 cut(s) 96, 102, 264, 665, 1019
MbiI CCGCTC 1 cut(s) 1198
MboI GATC 5 cut(s) 94, 100, 262, 663, 1017
MboII GAAGA 8 cut(s) 196, 456, 637, 928, 988, 1262, 1412, 1443
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 474, 1506
MlyI GAGTC 3 cut(s) 17, 1108, 1112
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 7 cut(s) 44, 657, 678, 1056, 1295, 1440, 1445
MspA1I CMGCKG 2 cut(s) 73, 131
MspI CCGG 2 cut(s) 383, 761
MspR9I CCNGG 1 cut(s) 608
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 875
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 831, 870
NdeII GATC 5 cut(s) 94, 100, 262, 663, 1017
NlaIII CATG 9 cut(s) 151, 236, 774, 825, 842, 1034, 1073, 1244, 1394
NlaIV GGNNCC 2 cut(s) 364, 759
NspI RCATGY 3 cut(s) 774, 825, 1034
PaeR7I CTCGAG 1 cut(s) 1082
PciI ACATGT 1 cut(s) 770
PctI GAATGC 1 cut(s) 875
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 4 cut(s) 447, 557, 722, 1159
PkrI GCNGC 7 cut(s) 75, 78, 130, 399, 1144, 1147, 1200
PleI GAGTC 3 cut(s) 16, 1107, 1112
PpsI GAGTC 3 cut(s) 16, 1107, 1112
Ppu21I YACGTR 2 cut(s) 1280, 1430
PscI ACATGT 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 364, 759
PspPI GGNCC 1 cut(s) 758
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 2 cut(s) 73, 131
RsaI GTAC 5 cut(s) 326, 352, 364, 817, 1369
RsaNI GTAC 5 cut(s) 325, 351, 363, 816, 1368
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 7 cut(s) 44, 657, 678, 1056, 1295, 1440, 1445
SatI GCNGC 7 cut(s) 74, 77, 129, 398, 1143, 1146, 1199
Sau3AI GATC 5 cut(s) 94, 100, 262, 663, 1017
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 3 cut(s) 352, 817, 1369
SchI GAGTC 3 cut(s) 17, 1108, 1112
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 2 cut(s) 474, 1506
SfaNI GCATC 2 cut(s) 9, 607
SfcI CTRYAG 2 cut(s) 126, 501
Sfr274I CTCGAG 1 cut(s) 1082
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 1082
SmlI CTYRAG 5 cut(s) 56, 467, 473, 1082, 1202
SmoI CTYRAG 5 cut(s) 56, 467, 473, 1082, 1202
SsiI CCGC 4 cut(s) 5, 620, 1148, 1198
SspI AATATT 1 cut(s) 1095
SspMI CTAG 2 cut(s) 248, 765
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 1 cut(s) 606
TaaI ACNGT 5 cut(s) 333, 893, 1114, 1179, 1372
TaiI ACGT 2 cut(s) 1282, 1432
TaqI TCGA 5 cut(s) 258, 662, 1083, 1102, 1489
TaqII GACCGA 2 cut(s) 708, 1290
TatI WGTACW 3 cut(s) 350, 815, 1367
TauI GCSGC 1 cut(s) 1201
TfiI GAWTC 4 cut(s) 447, 557, 722, 1159
Tru1I TTAA 7 cut(s) 44, 657, 678, 1056, 1295, 1440, 1445
Tru9I TTAA 7 cut(s) 44, 657, 678, 1056, 1295, 1440, 1445
TscAI CASTG 2 cut(s) 1027, 1117
TseI GCWGC 6 cut(s) 73, 76, 128, 397, 1142, 1145
TspDTI ATGAA 7 cut(s) 108, 150, 581, 765, 882, 1058, 1395
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 2 cut(s) 1027, 1117
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 2 cut(s) 747, 983
XceI RCATGY 3 cut(s) 774, 825, 1034
XhoI CTCGAG 1 cut(s) 1082
XmiI GTMKAC 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 640
XspI CTAG 2 cut(s) 248, 765
ZrmI AGTACT 3 cut(s) 352, 817, 1369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.