Rh2DG008900

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006173
Physical Location & Seq
Reverse (-)
523287 .. 526462
3176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG008900.1

Sequence Viewer

Length: 1629 bp
ATGGCGGAGTCAGTTGGGATGCCTGAGACTGATATAACGGCGTTAACCGAAGCGCTCAAGTCTCAGCAACAGCTGCTGCAGATGCTTTATGCTGATCTTGATCAGGAAAGAGAAGCTTCATCTACTGCAGCTGATGAAGCTCTATCGATGATACTGCGTTTGCAAGGTGAGAAGTCTGCTGTGAAGATGGAGGCCAACCAATACAAGAGATTGGCAGAGGAAAAGATATGTCATGCCGAGGAAGCTCTAGCGATTTTCGAGGATCTTATTTATCAGAAGGAAATGGAAATTGCTTCTTTGGAGTTTCAGCTTCAGGCTTATAGGTACAAACTGTTGAGCTTGGGTGTCAGTACAGAATTAGGTACCACCGAAAATGTGTATCCGGAAAACTTGTTGCAGCAAAGAGGTGATGGGGAAGCGAGTGGTGTTAGTGGGGCAATTAGAAGAATCAACTCCCTGCCTCCACTTGAGCTCAAGGATTTCCTCAACAAGAAATGCCCTGTAGAAAGAGAGAGTGAAAGTCTTCCGAAAGAAAAACCTATGATACCAAAGCCAGAATCTTGTATGAAAAAGGGAGACAAAAAGGTGGACAAGGAAGTTACTTTCCAGGGCATTGATGCAGAGAAGAAATCGAGAAATGCTTCCAATGGCAACCTTAATTCGATCTGGGAGCAGATTAAGAAGTTAGACGACCGAGTGAGAGAGATATCAGATTGTAAAGATTCCGGTGGTATAGGAAAATCTGGAATTTTGAAGGGTCCGGGTAGGACATGTTCATTACCTCCAATTCCGAAAGTAATCACAAGCACATCCCATGATACAGAAAGTGAAGATATTATTGCTACCTTTGAAAGAGTTGATCATAATGAGAATGTACAGGAAACGGAGGCAATTGTTAGTACTACATGCTCAACTGGCGTCCATGATGTTTTTGAAGTTCCACAGAGCTATGAATGCAGTCCTAAAACTTTGGACAGTCCTAAATCTTTTGAAGGTGAGAAGAAAGAGTTTGATAAATTGACATTTGGTGTTGAGAGTTCTAAAGCTTTTGAAATTGAGAAGAAAGAATTTATTGGCAAAGATGATTTGATATGTGATGAGATTAGTGAAGTGTATGTTCAAGATGAGACAGAGAGGGTTAAGAAAAGGTTTCATGGCAAGTTGCTCGAGAATAGAATATTGAGTCGAACGACCACTGTTGACTCAACTGCAAGGGAGAGAGTGGCTGCTGCGGTTACTGAGAATCAAGCCAGATTTCAACAGTTGTGGAGGAGGATAGAGCGGCTTGAGGGAGAGAGAAGTAGCGTAAGGAACGAAATAAGTCATGCAGGAGAAGAGGAGTTGAAGTTGTTTAGGGATATACGTGAGCAACTTAACTTAATTCGGTCTGAAATACATATTTGGAAGCCTAAGCAACCTCCTCCACAGGATGACGAGAACTTACACAGAAGTGTAAGGAATGAAATAAGTCATGCAGGAGAAGAGGATTTGAAGTTGTTTAGGGATATACGTGAGCAACTTAACTTAATTTGCTCTGAAATACATACTTGGAAGCCTAAGCAACCTCCTCGACAGGATGACGAGCCCTTACATATTGTTCAAGAGGCAATGCTACACTTTTGGCTCTGA

Protein Analysis

542

Amino Acids

61.79

Weight (kDa)

5.16

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 14 - 104 1e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccBSI CCGCTC 1 cut(s) 1282
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 3 cut(s) 5, 1232, 1282
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 747, 1067
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 5 cut(s) 326, 352, 364, 876, 901
AfeI AGCGCT 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 770
AjnI CCWGG 1 cut(s) 606
AleI CACNNNNGTG 1 cut(s) 1449
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 20, 66, 570, 1121
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 1166
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 6 cut(s) 73, 76, 128, 397, 1226, 1229
ApoI RAATTY 2 cut(s) 747, 1067
Asp700I GAANNNNTTC 2 cut(s) 522, 640
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 758
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 3 cut(s) 179, 419, 1007
AvaI CYCGRG 1 cut(s) 1166
AvaII GGWCC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 2 cut(s) 474, 1587
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
BccI CCATC 2 cut(s) 181, 404
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 6 cut(s) 136, 170, 1147, 1181, 1550, 1584
BciT130I CCWGG 1 cut(s) 608
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 2 cut(s) 100, 859
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 4 cut(s) 20, 66, 570, 1121
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 3 cut(s) 77, 126, 501
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
BlsI GCNGC 7 cut(s) 75, 78, 130, 399, 1228, 1231, 1284
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 2 cut(s) 608, 762
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 1166
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 2 cut(s) 364, 759
BmrFI CCNGG 2 cut(s) 608, 762
BmsI GCATC 3 cut(s) 9, 72, 607
BpiI GAAGAC 1 cut(s) 515
Bpu10I CCTNAGC 2 cut(s) 1410, 1557
BpuEI CTTGAG 4 cut(s) 41, 458, 488, 1307
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 146
BsaAI YACGTR 2 cut(s) 1364, 1511
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 2 cut(s) 237, 607
BsaWI WCCGGW 2 cut(s) 382, 725
BsaXI ACNNNNNCTCC 2 cut(s) 1331, 1361
Bse1I ACTGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 1614
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 608
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 237, 607
BseGI GGATG 4 cut(s) 24, 809, 1435, 1582
BseJI GATNNNNATC 1 cut(s) 99
BseMI GCAATG 1 cut(s) 1614
BseMII CTCAG 3 cut(s) 15, 77, 1230
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 4 cut(s) 1285, 1352, 1410, 1557
BseXI GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 1166
BsiSI CCGG 3 cut(s) 383, 726, 761
BsmAI GTCTC 4 cut(s) 20, 66, 570, 1121
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 1166
Bsp1286I GDGCHC 2 cut(s) 474, 1587
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 874
Bsp143I GATC 5 cut(s) 94, 100, 262, 663, 859
BspACI CCGC 3 cut(s) 5, 1232, 1282
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 3 cut(s) 16, 76, 1231
BspDI ATCGAT 1 cut(s) 146
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 364, 759
BspMAI CTGCAG 2 cut(s) 81, 130
BspPI GGATC 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 1282
BsrDI GCAATG 1 cut(s) 1614
BsrGI TGTACA 1 cut(s) 874
BsrI ACTGG 1 cut(s) 919
BssECI CCNNGG 2 cut(s) 237, 607
BssMI GATC 5 cut(s) 94, 100, 262, 663, 859
BssNI GRCGYC 1 cut(s) 918
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 4 cut(s) 333, 977, 1198, 1263
Bst6I CTCTTC 2 cut(s) 1329, 1476
BstACI GRCGYC 1 cut(s) 918
BstAPI GCANNNNNTGC 1 cut(s) 73
BstAUI TGTACA 1 cut(s) 874
BstBAI YACGTR 2 cut(s) 1364, 1511
BstDEI CTNAG 5 cut(s) 24, 63, 1239, 1410, 1557
BstF5I GGATG 4 cut(s) 24, 809, 1435, 1582
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 5 cut(s) 97, 103, 265, 666, 862
BstMAI GTCTC 4 cut(s) 20, 66, 570, 1121
BstMBI GATC 5 cut(s) 94, 100, 262, 663, 859
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
BstNI CCWGG 1 cut(s) 608
BstNSI RCATGY 2 cut(s) 774, 909
BstSCI CCNGG 2 cut(s) 606, 760
BstSFI CTRYAG 3 cut(s) 77, 126, 501
BstV1I GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 146
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 194
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 4 cut(s) 24, 809, 1435, 1582
BtsIMutI CAGTG 1 cut(s) 1194
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 758
ClaI ATCGAT 1 cut(s) 146
CseI GACGC 1 cut(s) 907
Csp6I GTAC 5 cut(s) 325, 351, 363, 875, 900
CspCI CAANNNNNGTGG 2 cut(s) 1247, 1282
CviAII CATG 8 cut(s) 233, 771, 815, 906, 923, 1154, 1325, 1472
CviQI GTAC 5 cut(s) 325, 351, 363, 875, 900
DdeI CTNAG 5 cut(s) 24, 63, 1239, 1410, 1557
DpnI GATC 5 cut(s) 96, 102, 264, 665, 861
DpnII GATC 5 cut(s) 94, 100, 262, 663, 859
Eam1104I CTCTTC 2 cut(s) 1329, 1476
EarI CTCTTC 2 cut(s) 1329, 1476
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 2 cut(s) 474, 1587
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 758
Eco47III AGCGCT 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 472
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 1166
EcoICRI GAGCTC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 708
EcoT38I GRGCYC 2 cut(s) 474, 1587
FaeI CATG 8 cut(s) 236, 774, 818, 909, 926, 1157, 1328, 1475
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FatI CATG 8 cut(s) 232, 770, 814, 905, 922, 1153, 1324, 1471
FbaI TGATCA 2 cut(s) 100, 859
Fnu4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
FokI GGATG 4 cut(s) 31, 796, 1442, 1589
FriOI GRGCYC 2 cut(s) 474, 1587
Fsp4HI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 54
GluI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 383, 726, 761
HgaI GACGC 1 cut(s) 907
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 918
Hin1II CATG 8 cut(s) 236, 774, 818, 909, 926, 1157, 1328, 1475
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 2 cut(s) 45, 1201
HindII GTYRAC 2 cut(s) 45, 1201
HindIII AAGCTT 2 cut(s) 114, 1044
HinfI GANTC 7 cut(s) 8, 447, 557, 722, 1183, 1202, 1243
HpaI GTTAAC 1 cut(s) 45
HpaII CCGG 3 cut(s) 383, 726, 761
HphI GGTGA 3 cut(s) 179, 419, 1007
Hpy166II GTNNAC 3 cut(s) 45, 589, 1201
Hpy188I TCNGA 7 cut(s) 276, 528, 712, 792, 1390, 1537, 1628
Hpy188III TCNNGA 8 cut(s) 98, 104, 383, 633, 744, 1121, 1168, 1601
Hpy8I GTNNAC 3 cut(s) 45, 589, 1201
HpyAV CCTTC 3 cut(s) 271, 748, 986
HpyCH4III ACNGT 4 cut(s) 333, 977, 1198, 1263
HpyCH4IV ACGT 2 cut(s) 1363, 1510
HpyCH4V TGCA 9 cut(s) 79, 128, 163, 397, 620, 957, 1211, 1328, 1475
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
HpyF3I CTNAG 5 cut(s) 24, 63, 1239, 1410, 1557
HpySE526I ACGT 2 cut(s) 1363, 1510
Hsp92I GRCGYC 1 cut(s) 918
Hsp92II CATG 8 cut(s) 236, 774, 818, 909, 926, 1157, 1328, 1475
HspAI GCGC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 382
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 2 cut(s) 100, 859
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 5 cut(s) 94, 100, 262, 663, 859
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 6 cut(s) 60, 63, 140, 409, 1213, 1216
LweI GCATC 3 cut(s) 9, 72, 607
MaeI CTAG 1 cut(s) 248
MaeII ACGT 2 cut(s) 1363, 1510
MaeIII GTNAC 2 cut(s) 598, 1234
MalI GATC 5 cut(s) 96, 102, 264, 665, 861
MbiI CCGCTC 1 cut(s) 1282
MboI GATC 5 cut(s) 94, 100, 262, 663, 859
MboII GAAGA 9 cut(s) 196, 456, 515, 637, 842, 1012, 1072, 1346, 1493
MfeI CAATTG 1 cut(s) 891
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 474, 1587
MlyI GAGTC 3 cut(s) 17, 1192, 1196
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 2 cut(s) 522, 640
MseI TTAA 8 cut(s) 44, 657, 678, 1140, 1374, 1379, 1521, 1526
MslI CAYNNNNRTG 1 cut(s) 1449
MspA1I CMGCKG 2 cut(s) 73, 131
MspI CCGG 3 cut(s) 383, 726, 761
MspR9I CCNGG 2 cut(s) 608, 762
MunI CAATTG 1 cut(s) 891
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 1 cut(s) 608
MwoI GCNNNNNNNGC 6 cut(s) 73, 82, 137, 242, 915, 954
NciI CCSGG 1 cut(s) 762
NdeII GATC 5 cut(s) 94, 100, 262, 663, 859
NlaIII CATG 8 cut(s) 236, 774, 818, 909, 926, 1157, 1328, 1475
NlaIV GGNNCC 2 cut(s) 364, 759
NmeAIII GCCGAG 1 cut(s) 262
NspI RCATGY 2 cut(s) 774, 909
OliI CACNNNNGTG 1 cut(s) 1449
PaeR7I CTCGAG 1 cut(s) 1166
PciI ACATGT 1 cut(s) 770
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 2 cut(s) 522, 640
PfeI GAWTC 4 cut(s) 447, 557, 722, 1243
PkrI GCNGC 7 cut(s) 75, 78, 130, 399, 1228, 1231, 1284
PleI GAGTC 3 cut(s) 16, 1191, 1196
PpsI GAGTC 3 cut(s) 16, 1191, 1196
Ppu21I YACGTR 2 cut(s) 1364, 1511
PscI ACATGT 1 cut(s) 770
Psp124BI GAGCTC 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 606
PspGI CCWGG 1 cut(s) 606
PspN4I GGNNCC 2 cut(s) 364, 759
PspPI GGNCC 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
PstI CTGCAG 2 cut(s) 81, 130
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 2 cut(s) 73, 131
RsaI GTAC 5 cut(s) 326, 352, 364, 876, 901
RsaNI GTAC 5 cut(s) 325, 351, 363, 875, 900
RseI CAYNNNNRTG 1 cut(s) 1449
SacI GAGCTC 1 cut(s) 474
SaqAI TTAA 8 cut(s) 44, 657, 678, 1140, 1374, 1379, 1521, 1526
SatI GCNGC 7 cut(s) 74, 77, 129, 398, 1227, 1230, 1283
Sau3AI GATC 5 cut(s) 94, 100, 262, 663, 859
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 3 cut(s) 17, 1192, 1196
ScrFI CCNGG 2 cut(s) 608, 762
SduI GDGCHC 2 cut(s) 474, 1587
SfaNI GCATC 3 cut(s) 9, 72, 607
SfcI CTRYAG 3 cut(s) 77, 126, 501
Sfr274I CTCGAG 1 cut(s) 1166
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 1166
SmiMI CAYNNNNRTG 1 cut(s) 1449
SmlI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
SmoI CTYRAG 5 cut(s) 56, 467, 473, 1166, 1286
SsiI CCGC 3 cut(s) 5, 1232, 1282
SspI AATATT 1 cut(s) 1179
SspMI CTAG 1 cut(s) 248
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 2 cut(s) 606, 760
TaaI ACNGT 4 cut(s) 333, 977, 1198, 1263
TaiI ACGT 2 cut(s) 1366, 1513
TaqI TCGA 7 cut(s) 146, 258, 632, 662, 1167, 1186, 1570
TaqII GACCGA 2 cut(s) 708, 1374
TatI WGTACW 3 cut(s) 350, 874, 899
TauI GCSGC 1 cut(s) 1285
TfiI GAWTC 4 cut(s) 447, 557, 722, 1243
Tru1I TTAA 8 cut(s) 44, 657, 678, 1140, 1374, 1379, 1521, 1526
Tru9I TTAA 8 cut(s) 44, 657, 678, 1140, 1374, 1379, 1521, 1526
TscAI CASTG 1 cut(s) 1201
TseI GCWGC 6 cut(s) 73, 76, 128, 397, 1226, 1229
TspDTI ATGAA 7 cut(s) 108, 150, 581, 765, 966, 1142, 1476
TspGWI ACGGA 1 cut(s) 899
TspRI CASTG 1 cut(s) 1201
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 2 cut(s) 747, 1067
XceI RCATGY 2 cut(s) 774, 909
XhoI CTCGAG 1 cut(s) 1166
XmnI GAANNNNTTC 2 cut(s) 522, 640
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.