pycom02g00370

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006173
Physical Location & Seq
Reverse (-)
229150 .. 230465
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00370.1

Sequence Viewer

Length: 1164 bp
ATGGTTGAATCTTTCCGCAGTGATTTTTCTCAACTGGGTTTTGTGAAGATCAAGTTTTTGCCTTTTACGCAGCATCAGGGTGGTGTGCCTTGTGGAATGCCTGAAACTGATATGACAACGCTAACCGAAGCGCTTAGAGCGCAACAACAACTACTGCAAAAGCTTTACTCTGATCTGGATCAGGAAAGAGAAGCATCATCTACTGCAGCTGATGAAGCTCTATCCATGATACTGCGTTTGCAAGGAGAAAAGTCCGCCATGAAGATGGAAGCAAATCAATACAAGAGATTGGCGGAGGAAAAGATCTGCCATGCCGAGGAAGCTCTTGCCATTTTCGAAGATCTCATTTATCAGAAGGAAATGGAAATTGCTTCGCTTGAGTTCCAACTTCAGGCTTATAGGTACAAGCTTTTAAGCATGGGCTGCAGCACGGAGCTAGGCGCTGGTGAAAATGTATATCCGGAGAATTTGTTGTTTCAAAGAAATGATTTCGGAAGTGCGGAAGTGGGTGTTAGTGGGACGATTAGAAGAATCAACTCTCGAGGTGATTTTGGCAATGGGGAAACGGGTGTTAGCGGGGCTATTAGAAGAATCAATTCTCTACCTCCACTTGAGCTCAAGGAATTGCTCAAAAGCACTGTGGAAAGAGAAAGAGATAAAGACAGAGATAAAGAAAGAGAACGAGAAAGAGACAGACCAATGATCCCCAAACCCGAACCCGCAACAAAAAAACAGGAGAAGAAGGTGGATCAGGAAGTCAATGTCCAGAGCATAGATGTTGAGAAGAAATCACCAAATTATGTGGGCGGGAACATGAACACTATCTGGGAGCAGATTAAAAAGTTAGACGCACGAGTGAAGGAGATATCCGATTGTAAGGATTATGGAAAATCTGCACTCTGGAAGGGTCCTCCATCCAGGACTTGTTCATTGCCTCCAATTCCGAAGATAAGCATAAGCCCAAAAAATGAGCCAGCCAGTGAAGAAATTATTGCTAGTCTACAGGGAAGGGAAGCAATTGATAGCCCTTCATGCTACTCAAGTGTCCATGATGTTTTCGAAGTCCCACAAAGTTATGAGAATCATAAAGCCGGTGAAGGAGAGAAGAAAGAACTTAATACATTGACAGTGGAAGTCGAGAACAGGTTTGGTAAGCCGACATAA

Protein Analysis

388

Amino Acids

43.63

Weight (kDa)

5.36

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 40 - 130 4.3e-33 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1000
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 7 cut(s) 16, 255, 293, 500, 576, 720, 807
AclWI GGATC 3 cut(s) 186, 697, 756
AcsI RAATTY 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 1 cut(s) 404
AfeI AGCGCT 1 cut(s) 132
AfiI CCNNNNNNNGG 2 cut(s) 316, 391
AgsI TTSAA 2 cut(s) 8, 479
AjnI CCWGG 1 cut(s) 917
AluBI AGCT 7 cut(s) 163, 209, 218, 323, 409, 436, 616
AluI AGCT 7 cut(s) 163, 209, 218, 323, 409, 436, 616
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 3 cut(s) 186, 697, 756
Ama87I CYCGRG 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 460
Aor51HI AGCGCT 1 cut(s) 132
ApeKI GCWGC 4 cut(s) 70, 206, 423, 426
ApoI RAATTY 1 cut(s) 466
Asp700I GAANNNNTTC 1 cut(s) 595
AspLEI GCGC 3 cut(s) 133, 142, 443
AspS9I GGNCC 1 cut(s) 908
AsuHPI GGTGA 4 cut(s) 458, 557, 783, 1106
AsuII TTCGAA 2 cut(s) 336, 1059
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 1 cut(s) 908
BanII GRGCYC 1 cut(s) 618
BauI CACGAG 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 618
BbvI GCAGC 4 cut(s) 82, 218, 410, 438
BccI CCATC 2 cut(s) 259, 922
BciT130I CCWGG 1 cut(s) 919
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 2 cut(s) 437, 996
BfmI CTRYAG 3 cut(s) 204, 424, 1001
BfoI RGCGCY 2 cut(s) 134, 444
BglII AGATCT 2 cut(s) 303, 340
BisI GCNGC 4 cut(s) 71, 207, 424, 427
BlsI GCNGC 4 cut(s) 72, 208, 425, 428
Bme1390I CCNGG 1 cut(s) 919
Bme18I GGWCC 1 cut(s) 908
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 1 cut(s) 909
BmrFI CCNGG 1 cut(s) 919
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 82, 203
BmuI ACTGGG 1 cut(s) 44
Bpu14I TTCGAA 2 cut(s) 336, 1059
BpuEI CTTGAG 4 cut(s) 398, 602, 632, 1024
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 391
Bse118I RCCGGY 1 cut(s) 1091
Bse1I ACTGG 2 cut(s) 39, 978
Bse3DI GCAATG 2 cut(s) 562, 929
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 919
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 1 cut(s) 914
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 316, 391
BseMI GCAATG 2 cut(s) 562, 929
BseNI ACTGG 2 cut(s) 39, 978
BseXI GCAGC 4 cut(s) 82, 218, 410, 438
BsgI GTGCAG 1 cut(s) 879
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 2 cut(s) 461, 1092
BslFI GGGAC 2 cut(s) 532, 1049
BslI CCNNNNNNNGG 2 cut(s) 316, 391
BsmAI GTCTC 1 cut(s) 684
BsmFI GGGAC 2 cut(s) 532, 1049
BsmI GAATGC 1 cut(s) 102
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 2 cut(s) 336, 1059
Bsp1286I GDGCHC 1 cut(s) 618
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
BspACI CCGC 7 cut(s) 16, 255, 293, 500, 576, 720, 807
BspEI TCCGGA 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 909
BspMAI CTGCAG 2 cut(s) 208, 428
BspPI GGATC 3 cut(s) 186, 697, 756
BspT104I TTCGAA 2 cut(s) 336, 1059
BsrDI GCAATG 2 cut(s) 562, 929
BsrFI RCCGGY 1 cut(s) 1091
BsrI ACTGG 2 cut(s) 39, 978
BssAI RCCGGY 1 cut(s) 1091
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
BssSI CACGAG 1 cut(s) 852
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 919
Bst4CI ACNGT 2 cut(s) 640, 1129
BstAPI GCANNNNNTGC 1 cut(s) 423
BstBI TTCGAA 2 cut(s) 336, 1059
BstC8I GCNNGC 1 cut(s) 975
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 1 cut(s) 914
BstH2I RGCGCY 2 cut(s) 134, 444
BstHHI GCGC 3 cut(s) 133, 142, 443
BstKTI GATC 7 cut(s) 51, 175, 181, 306, 343, 705, 751
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
BstMWI GCNNNNNNNGC 7 cut(s) 67, 137, 139, 215, 320, 423, 1032
BstNI CCWGG 1 cut(s) 919
BstSCI CCNGG 1 cut(s) 917
BstSFI CTRYAG 3 cut(s) 204, 424, 1001
BstV1I GCAGC 4 cut(s) 82, 218, 410, 438
BstX2I RGATCY 2 cut(s) 303, 340
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 2 cut(s) 303, 340
BtsCI GGATG 1 cut(s) 914
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 4 cut(s) 25, 636, 985, 1134
Cac8I GCNNGC 1 cut(s) 975
CfoI GCGC 3 cut(s) 133, 142, 443
Cfr10I RCCGGY 1 cut(s) 1091
Cfr13I GGNCC 1 cut(s) 908
CseI GACGC 1 cut(s) 857
Csp6I GTAC 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 783, 818
CviAII CATG 7 cut(s) 226, 259, 311, 418, 814, 1032, 1049
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 1 cut(s) 134
DpnI GATC 7 cut(s) 50, 174, 180, 305, 342, 704, 750
DpnII GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
EciI GGCGGA 2 cut(s) 244, 308
Ecl136II GAGCTC 1 cut(s) 616
Eco24I GRGCYC 1 cut(s) 618
Eco32I GATATC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 908
Eco47III AGCGCT 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 616
EcoO109I RGGNCCY 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 917
EcoRV GATATC 1 cut(s) 867
EcoT38I GRGCYC 1 cut(s) 618
FaeI CATG 7 cut(s) 229, 262, 314, 421, 817, 1035, 1052
FaqI GGGAC 2 cut(s) 532, 1049
FatI CATG 7 cut(s) 225, 258, 310, 417, 813, 1031, 1048
FauI CCCGC 3 cut(s) 569, 727, 800
FblI GTMKAC 1 cut(s) 1000
Fnu4HI GCNGC 4 cut(s) 71, 207, 424, 427
FokI GGATG 1 cut(s) 901
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 4 cut(s) 71, 207, 424, 427
FspBI CTAG 2 cut(s) 437, 996
GlaI GCGC 3 cut(s) 132, 141, 442
GluI GCNGC 4 cut(s) 71, 207, 424, 427
HaeII RGCGCY 2 cut(s) 134, 444
HapII CCGG 2 cut(s) 461, 1092
HgaI GACGC 1 cut(s) 857
HhaI GCGC 3 cut(s) 133, 142, 443
Hin1II CATG 7 cut(s) 229, 262, 314, 421, 817, 1035, 1052
Hin6I GCGC 3 cut(s) 131, 140, 441
HinP1I GCGC 3 cut(s) 131, 140, 441
HindIII AAGCTT 2 cut(s) 161, 407
HinfI GANTC 4 cut(s) 8, 531, 591, 1081
HpaII CCGG 2 cut(s) 461, 1092
HphI GGTGA 4 cut(s) 458, 557, 783, 1106
Hpy166II GTNNAC 1 cut(s) 1001
Hpy188I TCNGA 5 cut(s) 172, 354, 494, 871, 945
Hpy188III TCNNGA 8 cut(s) 176, 182, 461, 540, 752, 766, 901, 1138
Hpy8I GTNNAC 1 cut(s) 1001
HpyAV CCTTC 7 cut(s) 349, 736, 853, 898, 1002, 1038, 1091
HpyCH4III ACNGT 2 cut(s) 640, 1129
HpyCH4V TGCA 5 cut(s) 157, 206, 241, 426, 896
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 137, 139, 215, 320, 423, 1032
HpyF3I CTNAG 1 cut(s) 134
Hsp92II CATG 7 cut(s) 229, 262, 314, 421, 817, 1035, 1052
HspAI GCGC 3 cut(s) 131, 140, 441
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
LmnI GCTCC 2 cut(s) 433, 829
Lsp1109I GCAGC 4 cut(s) 82, 218, 410, 438
LweI GCATC 2 cut(s) 82, 203
MaeI CTAG 2 cut(s) 437, 996
MalI GATC 7 cut(s) 50, 174, 180, 305, 342, 704, 750
MboI GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 2 cut(s) 303, 340
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 8 cut(s) 366, 466, 595, 623, 796, 939, 987, 1017
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 6 cut(s) 289, 310, 536, 615, 921, 945
MroI TCCGGA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 595
MseI TTAA 3 cut(s) 413, 837, 1116
MslI CAYNNNNRTG 2 cut(s) 78, 263
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 2 cut(s) 461, 1092
MspR9I CCNGG 1 cut(s) 919
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 1 cut(s) 919
MwoI GCNNNNNNNGC 7 cut(s) 67, 137, 139, 215, 320, 423, 1032
NdeII GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
NlaIII CATG 7 cut(s) 229, 262, 314, 421, 817, 1035, 1052
NlaIV GGNNCC 1 cut(s) 909
NmeAIII GCCGAG 1 cut(s) 340
NspV TTCGAA 2 cut(s) 336, 1059
PaeR7I CTCGAG 1 cut(s) 540
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 4 cut(s) 8, 531, 591, 1081
PfoI TCCNGGA 1 cut(s) 917
PkrI GCNGC 4 cut(s) 72, 208, 425, 428
PpuMI RGGWCCY 1 cut(s) 908
Psp124BI GAGCTC 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 1 cut(s) 917
PspGI CCWGG 1 cut(s) 917
PspN4I GGNNCC 1 cut(s) 909
PspPI GGNCC 1 cut(s) 908
PspPPI RGGWCCY 1 cut(s) 908
PstI CTGCAG 2 cut(s) 208, 428
PsuI RGATCY 2 cut(s) 303, 340
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 2 cut(s) 78, 263
SacI GAGCTC 1 cut(s) 618
SaqAI TTAA 3 cut(s) 413, 837, 1116
SatI GCNGC 4 cut(s) 71, 207, 424, 427
Sau3AI GATC 7 cut(s) 48, 172, 178, 303, 340, 702, 748
Sau96I GGNCC 1 cut(s) 908
ScrFI CCNGG 1 cut(s) 919
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 2 cut(s) 82, 203
SfcI CTRYAG 3 cut(s) 204, 424, 1001
Sfr274I CTCGAG 1 cut(s) 540
SfuI TTCGAA 2 cut(s) 336, 1059
SinI GGWCC 1 cut(s) 908
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 2 cut(s) 78, 263
SmlI CTYRAG 5 cut(s) 377, 540, 611, 617, 1039
SmoI CTYRAG 5 cut(s) 377, 540, 611, 617, 1039
Sse9I AATT 8 cut(s) 366, 466, 595, 623, 796, 939, 987, 1017
SsiI CCGC 7 cut(s) 16, 255, 293, 500, 576, 720, 807
SspMI CTAG 2 cut(s) 437, 996
SstI GAGCTC 1 cut(s) 618
StyD4I CCNGG 1 cut(s) 917
TaaI ACNGT 2 cut(s) 640, 1129
TaqI TCGA 4 cut(s) 336, 541, 1059, 1137
TasI AATT 8 cut(s) 366, 466, 595, 623, 796, 939, 987, 1017
TfiI GAWTC 4 cut(s) 8, 531, 591, 1081
Tru1I TTAA 3 cut(s) 413, 837, 1116
Tru9I TTAA 3 cut(s) 413, 837, 1116
TscAI CASTG 4 cut(s) 25, 643, 985, 1134
TseI GCWGC 4 cut(s) 70, 206, 423, 426
TspDTI ATGAA 5 cut(s) 228, 275, 830, 918, 1020
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 4 cut(s) 25, 643, 985, 1134
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 1000
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 2 cut(s) 437, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.