MD02G1006300.v1.1

UPF0057 membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007615
Physical Location & Seq
Reverse (-)
438209 .. 438896
688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1006300.v1.1.491

Sequence Viewer

Length: 234 bp
ATGCCGAGTCGCTGCGAGATCTTTTGCGAGATTCTGATCGCAATTTTGCTCCCTCCGCTGGGCGTTTGTCTCCGGCACGGCTGCTGCACCGTGGAGTTCTGCATATGCTTGCTGCTGACGCTGCTGGGGTACATTCCGGGAATCATCTACGCCCTCTACGCCATCGTCTTCATCAATCGCGACGAGTACTTCGATGAGTACCGGCGCCCCCTCTACGCCTCCTCCTCGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pmp3

Protein Analysis

78

Amino Acids

8.8

Weight (kDa)

4.87

Isoelectric Point (pI)

68.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pmp3 PF01679 8 - 56 1.8e-19 Proteolipid membrane potential modulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 204
AccII CGCG 1 cut(s) 180
AciI CCGC 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 205
AfaI GTAC 3 cut(s) 131, 188, 200
AfiI CCNNNNNNNGG 2 cut(s) 58, 59
Alw26I GTCTC 1 cut(s) 74
ApeKI GCWGC 5 cut(s) 12, 81, 84, 112, 121
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AspLEI GCGC 1 cut(s) 207
AsuC2I CCSGG 1 cut(s) 138
BanI GGYRCC 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 160
BbvI GCAGC 4 cut(s) 68, 71, 99, 108
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 94
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 74
BfoI RGCGCY 1 cut(s) 208
BglII AGATCT 1 cut(s) 18
BisI GCNGC 5 cut(s) 13, 82, 85, 113, 122
BlsI GCNGC 5 cut(s) 14, 83, 86, 114, 123
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 90, 225
BsaXI ACNNNNNCTCC 1 cut(s) 206
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 59
Bse118I RCCGGY 1 cut(s) 201
Bse8I GATNNNNATC 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 90, 225
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 2 cut(s) 58, 59
BseRI GAGGAG 2 cut(s) 211, 214
BseXI GCAGC 4 cut(s) 68, 71, 99, 108
BseYI CCCAGC 2 cut(s) 58, 124
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 180
BshNI GGYRCC 1 cut(s) 204
BsiSI CCGG 3 cut(s) 73, 137, 202
BslI CCNNNNNNNGG 2 cut(s) 58, 59
BsmAI GTCTC 1 cut(s) 74
Bsp143I GATC 2 cut(s) 18, 36
Bsp68I TCGCGA 1 cut(s) 180
BspACI CCGC 1 cut(s) 56
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 204
BsrFI RCCGGY 1 cut(s) 201
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 2 cut(s) 90, 225
BssMI GATC 2 cut(s) 18, 36
BssNI GRCGYC 1 cut(s) 205
Bst4CI ACNGT 1 cut(s) 91
BstACI GRCGYC 1 cut(s) 205
BstC8I GCNNGC 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 90
BstFNI CGCG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 2 cut(s) 21, 39
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 2 cut(s) 18, 36
BstMWI GCNNNNNNNGC 4 cut(s) 55, 118, 121, 158
BstSCI CCNGG 1 cut(s) 136
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 68, 71, 99, 108
BstV2I GAAGAC 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BtgI CCRYGG 1 cut(s) 90
BtuMI TCGCGA 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 110
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 201
CseI GACGC 1 cut(s) 127
Csp6I GTAC 3 cut(s) 130, 187, 199
CviJI RGCY 1 cut(s) 81
CviKI_1 RGCY 1 cut(s) 81
CviQI GTAC 3 cut(s) 130, 187, 199
DinI GGCGCC 1 cut(s) 206
DpnI GATC 2 cut(s) 20, 38
DpnII GATC 2 cut(s) 18, 36
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
FaiI YATR 2 cut(s) 104, 106
FauNDI CATATG 1 cut(s) 104
Fnu4HI GCNGC 5 cut(s) 13, 82, 85, 113, 122
Fsp4HI GCNGC 5 cut(s) 13, 82, 85, 113, 122
GlaI GCGC 1 cut(s) 206
GluI GCNGC 5 cut(s) 13, 82, 85, 113, 122
GsaI CCCAGC 2 cut(s) 62, 128
HaeII RGCGCY 1 cut(s) 208
HapII CCGG 3 cut(s) 73, 137, 202
HgaI GACGC 1 cut(s) 127
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 205
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 3 cut(s) 7, 31, 141
HpaII CCGG 3 cut(s) 73, 137, 202
Hpy188I TCNGA 2 cut(s) 36, 229
Hpy188III TCNNGA 1 cut(s) 179
Hpy99I CGWCG 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4V TGCA 2 cut(s) 87, 102
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 118, 121, 158
Hsp92I GRCGYC 1 cut(s) 205
HspAI GCGC 1 cut(s) 205
KasI GGCGCC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 18, 36
LmnI GCTCC 1 cut(s) 54
LpnPI CCDG 5 cut(s) 44, 86, 110, 150, 215
Lsp1109I GCAGC 4 cut(s) 68, 71, 99, 108
MalI GATC 2 cut(s) 20, 38
MboI GATC 2 cut(s) 18, 36
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 18
MluCI AATT 1 cut(s) 42
Mly113I GGCGCC 1 cut(s) 205
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 5 cut(s) 63, 164, 221, 229, 232
MseI TTAA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 3 cut(s) 73, 137, 202
MspR9I CCNGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 55, 118, 121, 158
NarI GGCGCC 1 cut(s) 205
NciI CCSGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 104
NdeII GATC 2 cut(s) 18, 36
NlaIV GGNNCC 1 cut(s) 206
NmeAIII GCCGAG 1 cut(s) 30
NruI TCGCGA 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 2 cut(s) 31, 141
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 5 cut(s) 14, 83, 86, 114, 123
PleI GAGTC 1 cut(s) 15
PluTI GGCGCC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 15
PspFI CCCAGC 2 cut(s) 58, 124
PspN4I GGNNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 18
RruI TCGCGA 1 cut(s) 180
RsaI GTAC 3 cut(s) 131, 188, 200
RsaNI GTAC 3 cut(s) 130, 187, 199
SaqAI TTAA 1 cut(s) 232
SatI GCNGC 5 cut(s) 13, 82, 85, 113, 122
Sau3AI GATC 2 cut(s) 18, 36
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 138
SfoI GGCGCC 1 cut(s) 206
Sse9I AATT 1 cut(s) 42
SsiI CCGC 1 cut(s) 56
SspDI GGCGCC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 192
TasI AATT 1 cut(s) 42
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 2 cut(s) 31, 141
Tru1I TTAA 1 cut(s) 232
Tru9I TTAA 1 cut(s) 232
TseI GCWGC 5 cut(s) 12, 81, 84, 112, 121
TspDTI ATGAA 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.