MD02G1011000.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007708
Physical Location & Seq
Reverse (-)
709312 .. 712354
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1011000.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGCTTCCTCCATAGCTCGGAGCTCAGCTCGTATAGCCGTTGTCGGCGATGTCCATGATCAGTGGAATCTAGAAGAAGATAGGAAAGCACTTCAACTGCTGCAGCCGGATTTGGTTCTTTTTACAGGTGATTTTGGTGAAGAGAACGTTGAACTCGTTAAGAGCGTTGCAGATCTCGATATTACTAAGGCAATTATTTTAGGAAACCATGACGCCTGGCATACAACACAGTTTTCTCGAAAGAAAAAAGACGGCGTTCAACTTCAGCTAGAATGTCTCGGTGAGGAGCATGTGGGTTATCGGCGTTTGGACTATCCTGCTATGAAGCTGAGTGTTGTTGGTGGGCGACCTTTTTCTTGTGGGGGAAATGAGTTTTTTCGACAAAAGCTTCTGTCTGCAAGGTTTGGGGTCCAAGACATGGAAGGAAGTGCTAAAAGAATATGCGAAGCAACTTCGGGTGCTCCTGAGGACCATTTGATCATATTTCTTGCACATAATGGACCCACAGGTCTTGGGTCTAACGTGGATGACATTTGTGGAAAAGATTGGGTGTTTGAAGGTGGAGATTATGGTGACCCAGATCTTGCACAAGCCCTGTCCCTCTTAAAAGAGACTGCAACAATCTGTGCTCCACTGGTGGTGTTTGGTCACATGCATAAAGAGCTAGCGCATGGAAATGGTCTTCGGAAAATGATTGTGGTTGGAGCCGACAACACTATATACCTCAACGGGGCTATTTTTCCTAGAGTAAAGAGACTGAACGTTGAGCAAGGAACACCGGAGTCCTGCGGCACCATGCGCGCCTTCACTTTAGTGGAGATCTTGGACGGAACAGTTGATAAAATCGTTGAAAGTTGGGTTTCAGTTGCCGGCGATAGGATCACATTGGAAGAGGAGCACATTTTGTTCAAAAGTGGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

308

Amino Acids

33.61

Weight (kDa)

5.17

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 11 - 220 2.9e-12 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 507
AccB1I GGYRCC 1 cut(s) 791
AccB7I CCANNNNNTGG 1 cut(s) 418
AccII CGCG 1 cut(s) 801
AciI CCGC 1 cut(s) 789
AclI AACGTT 2 cut(s) 147, 762
AclWI GGATC 1 cut(s) 887
AcuI CTGAAG 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 213
AfiI CCNNNNNNNGG 4 cut(s) 18, 418, 730, 876
AgsI TTSAA 6 cut(s) 95, 152, 260, 557, 851, 910
AjnI CCWGG 1 cut(s) 215
AleI CACNNNNGTG 1 cut(s) 812
AluBI AGCT 7 cut(s) 17, 24, 29, 268, 328, 388, 664
AluI AGCT 7 cut(s) 17, 24, 29, 268, 328, 388, 664
Alw21I GWGCWC 4 cut(s) 26, 463, 631, 900
Alw26I GTCTC 3 cut(s) 281, 605, 748
AlwI GGATC 1 cut(s) 887
ApeKI GCWGC 2 cut(s) 100, 103
AspLEI GCGC 3 cut(s) 670, 801, 803
AspS9I GGNCC 3 cut(s) 409, 469, 500
AsuHPI GGTGA 4 cut(s) 140, 149, 293, 584
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 3 cut(s) 409, 469, 500
AxyI CCTNAGG 1 cut(s) 465
BanI GGYRCC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 26
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 4 cut(s) 26, 463, 631, 900
BbvI GCAGC 2 cut(s) 87, 115
BceAI ACGGC 2 cut(s) 23, 268
BciT130I CCWGG 1 cut(s) 217
BclI TGATCA 2 cut(s) 58, 477
BcoDI GTCTC 3 cut(s) 281, 605, 748
BfaI CTAG 4 cut(s) 71, 269, 665, 744
BfmI CTRYAG 1 cut(s) 101
BglII AGATCT 3 cut(s) 172, 580, 819
BisI GCNGC 3 cut(s) 101, 104, 790
BlpI GCTNAGC 1 cut(s) 25
BlsI GCNGC 3 cut(s) 102, 105, 791
Bme1390I CCNGG 1 cut(s) 217
Bme18I GGWCC 3 cut(s) 409, 469, 500
BmgT120I GGNCC 3 cut(s) 409, 469, 500
BmiI GGNNCC 4 cut(s) 410, 502, 706, 793
BmrFI CCNGG 1 cut(s) 217
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 674
BplI GAGNNNNNCTC 2 cut(s) 13, 45
Bpu1102I GCTNAGC 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 778
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 418, 730, 876
Bse118I RCCGGY 1 cut(s) 869
Bse1I ACTGG 1 cut(s) 639
Bse21I CCTNAGG 1 cut(s) 465
BseBI CCWGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 4 cut(s) 18, 418, 730, 876
BseMII CTCAG 3 cut(s) 39, 320, 456
BseNI ACTGG 1 cut(s) 639
BsePI GCGCGC 1 cut(s) 799
BseRI GAGGAG 2 cut(s) 299, 908
BseXI GCAGC 2 cut(s) 87, 115
Bsh1236I CGCG 1 cut(s) 801
BshNI GGYRCC 1 cut(s) 791
BsiHKAI GWGCWC 4 cut(s) 26, 463, 631, 900
BsiSI CCGG 3 cut(s) 107, 779, 870
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 4 cut(s) 18, 418, 730, 876
BsmAI GTCTC 3 cut(s) 281, 605, 748
BsmFI GGGAC 1 cut(s) 583
Bsp1286I GDGCHC 4 cut(s) 26, 463, 631, 900
Bsp143I GATC 6 cut(s) 58, 172, 477, 580, 819, 879
Bsp1720I GCTNAGC 1 cut(s) 25
BspACI CCGC 1 cut(s) 789
BspCNI CTCAG 3 cut(s) 38, 321, 457
BspFNI CGCG 1 cut(s) 801
BspLI GGNNCC 4 cut(s) 410, 502, 706, 793
BspMAI CTGCAG 1 cut(s) 105
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 1 cut(s) 887
BspT107I GGYRCC 1 cut(s) 791
BsrFI RCCGGY 1 cut(s) 869
BsrI ACTGG 1 cut(s) 639
BssAI RCCGGY 1 cut(s) 869
BssHII GCGCGC 1 cut(s) 799
BssMI GATC 6 cut(s) 58, 172, 477, 580, 819, 879
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 2 cut(s) 231, 835
Bst6I CTCTTC 2 cut(s) 135, 885
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 666, 801, 871
BstDEI CTNAG 4 cut(s) 25, 186, 329, 465
BstEII GGTNACC 1 cut(s) 572
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 801
BstHHI GCGC 3 cut(s) 670, 801, 803
BstKTI GATC 6 cut(s) 61, 175, 480, 583, 822, 882
BstMAI GTCTC 3 cut(s) 281, 605, 748
BstMBI GATC 6 cut(s) 58, 172, 477, 580, 819, 879
BstMWI GCNNNNNNNGC 3 cut(s) 35, 661, 798
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 2 cut(s) 293, 655
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 215
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 1 cut(s) 801
BstV1I GCAGC 2 cut(s) 87, 115
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 3 cut(s) 172, 580, 819
BstYI RGATCY 3 cut(s) 172, 580, 819
Bsu36I CCTNAGG 1 cut(s) 465
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 2 cut(s) 68, 632
Cac8I GCNNGC 3 cut(s) 666, 801, 871
CfoI GCGC 3 cut(s) 670, 801, 803
Cfr10I RCCGGY 1 cut(s) 869
Cfr13I GGNCC 3 cut(s) 409, 469, 500
CseI GACGC 1 cut(s) 221
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviAII CATG 7 cut(s) 56, 209, 290, 418, 652, 671, 796
DdeI CTNAG 4 cut(s) 25, 186, 329, 465
DpnI GATC 6 cut(s) 60, 174, 479, 582, 821, 881
DpnII GATC 6 cut(s) 58, 172, 477, 580, 819, 879
DrdI GACNNNNNNGTC 1 cut(s) 507
DseDI GACNNNNNNGTC 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 135, 885
EarI CTCTTC 2 cut(s) 135, 885
Ecl136II GAGCTC 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 3 cut(s) 409, 469, 500
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 248
Eco81I CCTNAGG 1 cut(s) 465
Eco91I GGTNACC 1 cut(s) 572
EcoICRI GAGCTC 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 26
FaeI CATG 7 cut(s) 59, 212, 293, 421, 655, 674, 799
FaqI GGGAC 1 cut(s) 583
FatI CATG 7 cut(s) 55, 208, 289, 417, 651, 670, 795
FbaI TGATCA 2 cut(s) 58, 477
Fnu4HI GCNGC 3 cut(s) 101, 104, 790
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 101, 104, 790
FspBI CTAG 4 cut(s) 71, 269, 665, 744
GlaI GCGC 3 cut(s) 669, 800, 802
GluI GCNGC 3 cut(s) 101, 104, 790
HapII CCGG 3 cut(s) 107, 779, 870
HgaI GACGC 1 cut(s) 221
HhaI GCGC 3 cut(s) 670, 801, 803
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 7 cut(s) 59, 212, 293, 421, 655, 674, 799
Hin6I GCGC 3 cut(s) 668, 799, 801
HinP1I GCGC 3 cut(s) 668, 799, 801
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 2 cut(s) 67, 782
HpaII CCGG 3 cut(s) 107, 779, 870
HphI GGTGA 4 cut(s) 140, 149, 293, 584
Hpy188I TCNGA 2 cut(s) 21, 687
Hpy188III TCNNGA 4 cut(s) 71, 176, 237, 464
HpyAV CCTTC 3 cut(s) 416, 551, 814
HpyCH4III ACNGT 2 cut(s) 231, 835
HpyCH4IV ACGT 3 cut(s) 147, 522, 762
HpyCH4V TGCA 7 cut(s) 103, 170, 398, 491, 587, 617, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 661, 798
HpyF3I CTNAG 4 cut(s) 25, 186, 329, 465
HpySE526I ACGT 3 cut(s) 147, 522, 762
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 7 cut(s) 59, 212, 293, 421, 655, 674, 799
HspAI GCGC 3 cut(s) 668, 799, 801
KroI GCCGGC 1 cut(s) 869
KroNI GCCGGC 1 cut(s) 871
Ksp22I TGATCA 2 cut(s) 58, 477
Kzo9I GATC 6 cut(s) 58, 172, 477, 580, 819, 879
LmnI GCTCC 6 cut(s) 21, 286, 466, 634, 704, 895
Lsp1109I GCAGC 2 cut(s) 87, 115
MaeI CTAG 4 cut(s) 71, 269, 665, 744
MaeII ACGT 3 cut(s) 147, 522, 762
MaeIII GTNAC 2 cut(s) 572, 647
MalI GATC 6 cut(s) 60, 174, 479, 582, 821, 881
MboI GATC 6 cut(s) 58, 172, 477, 580, 819, 879
MboII GAAGA 5 cut(s) 86, 89, 152, 674, 902
MflI RGATCY 3 cut(s) 172, 580, 819
MhlI GDGCHC 4 cut(s) 26, 463, 631, 900
MluCI AATT 1 cut(s) 192
MlyI GAGTC 1 cut(s) 791
MmeI TCCRAC 1 cut(s) 682
MnlI CCTC 6 cut(s) 19, 277, 460, 611, 734, 886
Mph1103I ATGCAT 1 cut(s) 657
MroNI GCCGGC 1 cut(s) 869
MseI TTAA 2 cut(s) 159, 605
MslI CAYNNNNRTG 2 cut(s) 675, 812
MspI CCGG 3 cut(s) 107, 779, 870
MspR9I CCNGG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 217
MvnI CGCG 1 cut(s) 801
MwoI GCNNNNNNNGC 3 cut(s) 35, 661, 798
NaeI GCCGGC 1 cut(s) 871
NdeII GATC 6 cut(s) 58, 172, 477, 580, 819, 879
NgoMIV GCCGGC 1 cut(s) 869
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 7 cut(s) 59, 212, 293, 421, 655, 674, 799
NlaIV GGNNCC 4 cut(s) 410, 502, 706, 793
NmuCI GTSAC 2 cut(s) 572, 647
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 2 cut(s) 293, 655
OliI CACNNNNGTG 1 cut(s) 812
PauI GCGCGC 1 cut(s) 799
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 162
PdiI GCCGGC 1 cut(s) 871
PfeI GAWTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 3 cut(s) 102, 105, 791
PleI GAGTC 1 cut(s) 790
PpsI GAGTC 1 cut(s) 790
Psp124BI GAGCTC 1 cut(s) 26
Psp1406I AACGTT 2 cut(s) 147, 762
Psp6I CCWGG 1 cut(s) 215
PspEI GGTNACC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 4 cut(s) 410, 502, 706, 793
PspPI GGNCC 3 cut(s) 409, 469, 500
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 3 cut(s) 172, 580, 819
PteI GCGCGC 1 cut(s) 799
RseI CAYNNNNRTG 2 cut(s) 675, 812
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 159, 605
SatI GCNGC 3 cut(s) 101, 104, 790
Sau3AI GATC 6 cut(s) 58, 172, 477, 580, 819, 879
Sau96I GGNCC 3 cut(s) 409, 469, 500
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 1 cut(s) 217
SduI GDGCHC 4 cut(s) 26, 463, 631, 900
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 3 cut(s) 409, 469, 500
SmiMI CAYNNNNRTG 2 cut(s) 675, 812
Sse9I AATT 1 cut(s) 192
SsiI CCGC 1 cut(s) 789
SspMI CTAG 4 cut(s) 71, 269, 665, 744
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 215
TaaI ACNGT 2 cut(s) 231, 835
TaiI ACGT 3 cut(s) 150, 525, 765
TaqI TCGA 3 cut(s) 177, 238, 379
TasI AATT 1 cut(s) 192
TauI GCSGC 1 cut(s) 792
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 2 cut(s) 159, 605
Tru9I TTAA 2 cut(s) 159, 605
TscAI CASTG 2 cut(s) 68, 639
TseFI GTSAC 2 cut(s) 572, 647
TseI GCWGC 2 cut(s) 100, 103
Tsp45I GTSAC 2 cut(s) 572, 647
TspDTI ATGAA 1 cut(s) 338
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 68, 639
Van91I CCANNNNNTGG 1 cut(s) 418
VpaK11BI GGWCC 3 cut(s) 409, 469, 500
XbaI TCTAGA 1 cut(s) 70
XceI RCATGY 2 cut(s) 293, 655
XspI CTAG 4 cut(s) 71, 269, 665, 744
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.