Rh2BG016200

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007708
Physical Location & Seq
Reverse (-)
1173907 .. 1176992
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG016200.1

Sequence Viewer

Length: 831 bp
ATGCTGAGCTCATTAGTTCACGCGCCACCACTCTCTTTTCTCTCCGCCTCGCTTCCCCGCCTTTTCTCCACGCACAACGCCACGCGCGCCGCCATGGCCGCCGCCACCTCAGCTCGAATCGTAGTCGTCGGCGACGTCCATGATCAGTGGAATCTAGAAGACGACACGAAAGCTCTTCGATTTCTGCAGCCGGATTTGGTTCTTTTTACAGGTGATTTTGGTGAAGAGAACATTGAACTTGTTCGCAGCGTTGCAAATCTTGAATTTGCTAAAGCAGTTATTTTGGGAAACCATGATGCCTGGCATACAAAAAAGTTTTCAGGAAAGACAAAAGACGGCGTTCAACTTCAGCTAGACTGTCTAGGCGAGGAGCATGTAGCTTATAGATGTTTGGACTTTGCTACGGTAAAACTGAGCATTGTTGGTGGACGACCTTTTTCTAGTGGGGGAAACCAGTTATTTCGATTAAAGCTTCTGTCTGCAAGGTATGGAGTCCAAGACATGGATGGAAGTGCTAAGAGAATCTGCAAAGCAGCTTTGGGTGCTCCAGAGGACCATATGGTCATTATTCTTGCACACAATGGACCCACAGGTCTTGGTTGTAACTTGGATGACATATGTGGAAAAGACTGGGACTTTGGAGGTGGAGATCATGGTGATGCAGGTAATAATAAGCTTTTAGCTCTAGGTATATATCTCAAATTCTCTGGCTGCCAACCCTATAAAGGGTACGATGGTGGGAATTCAGTCTTCAGTATAGAGGTCTTTTGTTATTTCCCTGTAAACTTGGTGGATGTTTTATCTGGTTGTGGAAAGGAGATGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

276

Amino Acids

29.74

Weight (kDa)

5.82

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 39 - 201 2.8e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 560, 591
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 653
AccB7I CCANNNNNTGG 1 cut(s) 502
AccII CGCG 3 cut(s) 23, 85, 87
AciI CCGC 5 cut(s) 45, 58, 90, 99, 102
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 3 cut(s) 263, 701, 742
AcuI CTGAAG 2 cut(s) 332, 736
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 3 cut(s) 502, 725, 726
AgsI TTSAA 3 cut(s) 236, 263, 344
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 9 cut(s) 9, 113, 173, 352, 380, 472, 536, 676, 683
AluI AGCT 9 cut(s) 9, 113, 173, 352, 380, 472, 536, 676, 683
Alw21I GWGCWC 2 cut(s) 11, 547
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 4 cut(s) 187, 246, 533, 711
ApoI RAATTY 3 cut(s) 263, 701, 742
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 3 cut(s) 25, 87, 89
AspS9I GGNCC 2 cut(s) 553, 584
AsuHPI GGTGA 3 cut(s) 224, 233, 668
AvaII GGWCC 2 cut(s) 553, 584
BaeI ACNNNNGTAYC 2 cut(s) 721, 754
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 165, 742
Bbv12I GWGCWC 2 cut(s) 11, 547
BbvCI CCTCAGC 1 cut(s) 109
BbvI GCAGC 4 cut(s) 199, 258, 545, 698
BccI CCATC 2 cut(s) 500, 728
BceAI ACGGC 1 cut(s) 352
BciT130I CCWGG 1 cut(s) 301
BclI TGATCA 1 cut(s) 142
BfaI CTAG 5 cut(s) 155, 353, 362, 441, 686
BfmI CTRYAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 653
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 7 cut(s) 90, 99, 102, 188, 247, 534, 712
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 7 cut(s) 91, 100, 103, 189, 248, 535, 713
Bme1390I CCNGG 1 cut(s) 301
Bme18I GGWCC 2 cut(s) 553, 584
BmgT120I GGNCC 2 cut(s) 553, 584
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 1 cut(s) 301
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 2 cut(s) 286, 649
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 2 cut(s) 165, 742
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 109
Bpu1102I GCTNAGC 1 cut(s) 5
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 725, 726
Bse1I ACTGG 2 cut(s) 454, 635
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 3 cut(s) 511, 616, 799
BseLI CCNNNNNNNGG 3 cut(s) 502, 725, 726
BseMII CTCAG 2 cut(s) 123, 404
BseNI ACTGG 2 cut(s) 454, 635
BsePI GCGCGC 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 4 cut(s) 199, 258, 545, 698
Bsh1236I CGCG 3 cut(s) 23, 85, 87
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 2 cut(s) 11, 547
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 3 cut(s) 502, 725, 726
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 11, 547
Bsp143I GATC 2 cut(s) 142, 649
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 5 cut(s) 45, 58, 90, 99, 102
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 122, 405
BspFNI CGCG 3 cut(s) 23, 85, 87
BspLI GGNNCC 1 cut(s) 586
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 653
BspQI GCTCTTC 1 cut(s) 180
BsrI ACTGG 2 cut(s) 454, 635
BssECI CCNNGG 1 cut(s) 93
BssHII GCGCGC 1 cut(s) 85
BssMI GATC 2 cut(s) 142, 649
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 359, 406
Bst6I CTCTTC 2 cut(s) 180, 219
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 4 cut(s) 5, 109, 413, 516
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 3 cut(s) 511, 616, 799
BstFNI CGCG 3 cut(s) 23, 85, 87
BstHHI GCGC 3 cut(s) 25, 87, 89
BstKTI GATC 2 cut(s) 145, 652
BstMBI GATC 2 cut(s) 142, 649
BstMWI GCNNNNNNNGC 5 cut(s) 86, 95, 98, 110, 542
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 1 cut(s) 185
BstUI CGCG 3 cut(s) 23, 85, 87
BstV1I GCAGC 4 cut(s) 199, 258, 545, 698
BstV2I GAAGAC 2 cut(s) 165, 742
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 3 cut(s) 511, 616, 799
BtsIMutI CAGTG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 3 cut(s) 25, 87, 89
Cfr13I GGNCC 2 cut(s) 553, 584
Csp6I GTAC 1 cut(s) 730
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 6 cut(s) 94, 140, 293, 374, 502, 653
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 4 cut(s) 5, 109, 413, 516
DpnI GATC 2 cut(s) 144, 651
DpnII GATC 2 cut(s) 142, 649
DrdI GACNNNNNNGTC 2 cut(s) 560, 591
DseDI GACNNNNNNGTC 2 cut(s) 560, 591
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 180, 219
EarI CTCTTC 2 cut(s) 180, 219
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 553, 584
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 332, 736
EcoICRI GAGCTC 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 6 cut(s) 97, 143, 296, 377, 505, 656
FaqI GGGAC 1 cut(s) 647
FatI CATG 6 cut(s) 93, 139, 292, 373, 501, 652
FauI CCCGC 1 cut(s) 65
FauNDI CATATG 2 cut(s) 558, 617
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 7 cut(s) 90, 99, 102, 188, 247, 534, 712
FokI GGATG 3 cut(s) 518, 623, 806
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 7 cut(s) 90, 99, 102, 188, 247, 534, 712
FspBI CTAG 5 cut(s) 155, 353, 362, 441, 686
GlaI GCGC 3 cut(s) 24, 86, 88
GluI GCNGC 7 cut(s) 90, 99, 102, 188, 247, 534, 712
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 191
HhaI GCGC 3 cut(s) 25, 87, 89
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 6 cut(s) 97, 143, 296, 377, 505, 656
Hin6I GCGC 3 cut(s) 23, 85, 87
HinP1I GCGC 3 cut(s) 23, 85, 87
HindIII AAGCTT 2 cut(s) 470, 674
HinfI GANTC 5 cut(s) 117, 151, 492, 522, 823
HpaII CCGG 1 cut(s) 191
HphI GGTGA 3 cut(s) 224, 233, 668
Hpy166II GTNNAC 3 cut(s) 19, 428, 784
Hpy188III TCNNGA 4 cut(s) 155, 260, 321, 548
Hpy8I GTNNAC 3 cut(s) 19, 428, 784
Hpy99I CGWCG 2 cut(s) 131, 137
HpyCH4III ACNGT 2 cut(s) 359, 406
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 6 cut(s) 187, 254, 482, 528, 575, 662
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 95, 98, 110, 542
HpyF3I CTNAG 4 cut(s) 5, 109, 413, 516
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 6 cut(s) 97, 143, 296, 377, 505, 656
HspAI GCGC 3 cut(s) 23, 85, 87
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 2 cut(s) 142, 649
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 370, 550
Lsp1109I GCAGC 4 cut(s) 199, 258, 545, 698
LweI GCATC 2 cut(s) 286, 649
MaeI CTAG 5 cut(s) 155, 353, 362, 441, 686
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 602
MalI GATC 2 cut(s) 144, 651
MboI GATC 2 cut(s) 142, 649
MboII GAAGA 4 cut(s) 167, 170, 236, 742
MhlI GDGCHC 2 cut(s) 11, 547
MluCI AATT 3 cut(s) 263, 701, 742
MlyI GAGTC 1 cut(s) 501
MnlI CCTC 6 cut(s) 58, 118, 361, 544, 635, 754
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 1 cut(s) 657
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 3 cut(s) 23, 85, 87
MwoI GCNNNNNNNGC 5 cut(s) 86, 95, 98, 110, 542
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 2 cut(s) 558, 617
NdeII GATC 2 cut(s) 142, 649
NlaIII CATG 6 cut(s) 97, 143, 296, 377, 505, 656
NlaIV GGNNCC 1 cut(s) 586
NspI RCATGY 1 cut(s) 377
PauI GCGCGC 1 cut(s) 85
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 3 cut(s) 117, 151, 522
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 7 cut(s) 91, 100, 103, 189, 248, 535, 713
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 2 cut(s) 553, 584
PstI CTGCAG 1 cut(s) 189
PteI GCGCGC 1 cut(s) 85
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
RseI CAYNNNNRTG 1 cut(s) 657
SacI GAGCTC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 7 cut(s) 90, 99, 102, 188, 247, 534, 712
Sau3AI GATC 2 cut(s) 142, 649
Sau96I GGNCC 2 cut(s) 553, 584
SchI GAGTC 1 cut(s) 501
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 2 cut(s) 11, 547
SfaNI GCATC 2 cut(s) 286, 649
SfcI CTRYAG 1 cut(s) 185
SinI GGWCC 2 cut(s) 553, 584
SmiMI CAYNNNNRTG 1 cut(s) 657
Sse9I AATT 3 cut(s) 263, 701, 742
SsiI CCGC 5 cut(s) 45, 58, 90, 99, 102
SspMI CTAG 5 cut(s) 155, 353, 362, 441, 686
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 359, 406
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 115, 178, 463
TasI AATT 3 cut(s) 263, 701, 742
TauI GCSGC 3 cut(s) 92, 101, 104
TfiI GAWTC 3 cut(s) 117, 151, 522
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 1 cut(s) 152
TseI GCWGC 4 cut(s) 187, 246, 533, 711
TspRI CASTG 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 2 cut(s) 553, 584
XapI RAATTY 3 cut(s) 263, 701, 742
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 503
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 5 cut(s) 155, 353, 362, 441, 686
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.