Rroxscaffold_2G00154750

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007708
Physical Location & Seq
Forward (+)
91256529 .. 91262463
5935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154750.1

Sequence Viewer

Length: 1203 bp
ATGCTAAGCTCATTAGTACACGCGCCACCACTCGCTTTTCTCTCCGCCTCGCTTCCCCGCCTTTTCTCCACGCACAACGCCACGCGCGCCGCCATGGCCGCCGCCACCTCAGCTCGAATCGCAGTCGTCGGCGACGTCCATGATCAGTGGAATCTAGAAGACGACACGAAAGCTCTTCGATTTCTGCAGCCGGATTTGGTTCTTTTCACAGGCAGGTTCCACTCACCTTCACTATGCATCTACTTAACGTTTTTTGTGCCTCCCTCTTTCCATATTGGCAACTGCCTTCCACTTTCTTGTCTTTCCCAATTGCCATGGCTGGGTGAAGCTGGATTTACCTCTTGTTTCTGCGCCAACATGGCTGGTGATTTTGGTGAAGAGAACATTGAACTTGTTCGCAGCGTTGCAAATCTTGAATTTGCTAAAGCAGTTATTTTGGGAAACCATGATGCCTGGCATACAAAAAAGTTTTCAGGAAAGACAAAAGATGGCGTTCAACTTCAGCTAGACTGTCTTGGCGAGGAGCATGTAGCTTACAGATGTTTGGACTTTGCTACAGTAAAACTGAGCATTGTTGGTGGACGACCTTTTTCTAGTGGGGGAAACCAGTTATTTCGATTAAAGCTTCTGTCTGCAAGGTATGGAGTCCAAGACATGGATGGAAGTGCTAAAAGAATCTGCAAAGCAGCTTTGGGTGCTCCAGAGGACCATATGGTCATTGTTCTTGCACACAATGGACCCACAGGTCTTGGTTCTAACTTGGATGACATATGTGGAAAAGACTGGGACTTTGGAGGTGGAGATCATGGTGATGCAGATCTAGCACAAGCCCTGTCCCTCCTAAAAGAGTCCGGCAAAACCTGTGTTCCATTGGTAGTGTTTGGTCACATGCATAAACAGCTAGCAGGTGGAAATGGTCTTCGGAAAATGATCGTGGTTGGAGCTGACAATACTATATATTTGAATGCGGCCATTGTTCCTAGAGTCAAGAGACTAAATGATGAACAAGGAACTAGCAATAAAAGCTTCGTGAATAATGAACCCGGTCTTTCAACTCCGGAGTCCTGTGGCACCATTCGAGCCTTCACTTTAGTGGAGATTTTGGACGGAGGAGTAGATAAGATTGTTGAAACTTGGGTTTCTGTTGTGGGTGATAGGACCAAATTGGAAGAGGAGCACATGTTATTCAAAAGTGGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

400

Amino Acids

43.1

Weight (kDa)

6.05

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 39 - 299 8.8e-09 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 896
AasI GACNNNNNNGTC 2 cut(s) 713, 744
AatII GACGTC 1 cut(s) 138
Acc36I ACCTGC 2 cut(s) 204, 896
AccB1I GGYRCC 1 cut(s) 1070
AccB7I CCANNNNNTGG 1 cut(s) 655
AccII CGCG 3 cut(s) 23, 85, 87
AccIII TCCGGA 1 cut(s) 1057
AciI CCGC 6 cut(s) 45, 58, 90, 99, 102, 968
AclI AACGTT 1 cut(s) 248
AcoI YGGCCR 2 cut(s) 96, 969
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 320, 655
AflIII ACRYGT 1 cut(s) 1179
AgsI TTSAA 7 cut(s) 389, 416, 497, 964, 1053, 1130, 1189
AjnI CCWGG 1 cut(s) 452
AleI CACNNNNGTG 1 cut(s) 1091
Alw21I GWGCWC 2 cut(s) 700, 1179
Alw26I GTCTC 1 cut(s) 985
Aor13HI TCCGGA 1 cut(s) 1057
AoxI GGCC 2 cut(s) 96, 969
ApeKI GCWGC 3 cut(s) 187, 399, 686
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 773, 805
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 4 cut(s) 25, 87, 89, 353
AspS9I GGNCC 3 cut(s) 706, 737, 1158
AsuC2I CCSGG 1 cut(s) 1044
AsuHPI GGTGA 6 cut(s) 216, 335, 377, 386, 821, 1163
AsuNHI GCTAGC 1 cut(s) 901
AvaII GGWCC 3 cut(s) 706, 737, 1158
BanI GGYRCC 1 cut(s) 1070
BbsI GAAGAC 2 cut(s) 165, 911
Bbv12I GWGCWC 2 cut(s) 700, 1179
BbvCI CCTCAGC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 199, 411, 698
BccI CCATC 2 cut(s) 482, 653
BciT130I CCWGG 1 cut(s) 454
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 1 cut(s) 1044
BcoDI GTCTC 1 cut(s) 985
BfaI CTAG 7 cut(s) 155, 506, 594, 821, 902, 981, 1014
BfmI CTRYAG 2 cut(s) 185, 555
BfuAI ACCTGC 2 cut(s) 204, 896
BglI GCCNNNNNGGC 2 cut(s) 95, 359
BglII AGATCT 1 cut(s) 817
BisI GCNGC 7 cut(s) 90, 99, 102, 188, 400, 687, 969
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 7 cut(s) 91, 100, 103, 189, 401, 688, 970
Bme1390I CCNGG 2 cut(s) 454, 1044
Bme18I GGWCC 3 cut(s) 706, 737, 1158
BmgT120I GGNCC 3 cut(s) 706, 737, 1158
BmiI GGNNCC 3 cut(s) 218, 739, 1072
BmrFI CCNGG 2 cut(s) 454, 1044
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 3 cut(s) 246, 439, 802
BmtI GCTAGC 1 cut(s) 905
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 2 cut(s) 165, 911
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 109
Bpu1102I GCTNAGC 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 1044
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 93, 314
BsaWI WCCGGW 1 cut(s) 1057
BsaXI ACNNNNNCTCC 2 cut(s) 1166, 1196
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 655
Bse1I ACTGG 2 cut(s) 607, 788
Bse8I GATNNNNATC 1 cut(s) 816
BseAI TCCGGA 1 cut(s) 1057
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 2 cut(s) 93, 314
BseGI GGATG 2 cut(s) 664, 769
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 320, 655
BseMII CTCAG 2 cut(s) 123, 557
BseNI ACTGG 2 cut(s) 607, 788
BsePI GCGCGC 1 cut(s) 85
BseRI GAGGAG 3 cut(s) 536, 1125, 1187
BseXI GCAGC 3 cut(s) 199, 411, 698
BseYI CCCAGC 1 cut(s) 319
Bsh1236I CGCG 3 cut(s) 23, 85, 87
BshFI GGCC 2 cut(s) 98, 971
BshNI GGYRCC 1 cut(s) 1070
BsiHKAI GWGCWC 2 cut(s) 700, 1179
BsiSI CCGG 4 cut(s) 191, 852, 1044, 1058
BslFI GGGAC 2 cut(s) 800, 820
BslI CCNNNNNNNGG 2 cut(s) 320, 655
BsmAI GTCTC 1 cut(s) 985
BsmFI GGGAC 2 cut(s) 800, 820
BsmI GAATGC 1 cut(s) 970
BsnI GGCC 2 cut(s) 98, 971
Bsp1286I GDGCHC 2 cut(s) 700, 1179
Bsp13I TCCGGA 1 cut(s) 1057
Bsp143I GATC 4 cut(s) 142, 802, 817, 930
Bsp1720I GCTNAGC 1 cut(s) 5
Bsp19I CCATGG 2 cut(s) 93, 314
BspACI CCGC 6 cut(s) 45, 58, 90, 99, 102, 968
BspANI GGCC 2 cut(s) 98, 971
BspCNI CTCAG 2 cut(s) 122, 558
BspEI TCCGGA 1 cut(s) 1057
BspFNI CGCG 3 cut(s) 23, 85, 87
BspLI GGNNCC 3 cut(s) 218, 739, 1072
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 2 cut(s) 204, 896
BspOI GCTAGC 1 cut(s) 905
BspQI GCTCTTC 1 cut(s) 180
BspT107I GGYRCC 1 cut(s) 1070
BsrI ACTGG 2 cut(s) 607, 788
BssECI CCNNGG 2 cut(s) 93, 314
BssHII GCGCGC 1 cut(s) 85
BssMI GATC 4 cut(s) 142, 802, 817, 930
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 93, 314
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 512, 559
Bst6I CTCTTC 3 cut(s) 180, 372, 1164
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 87, 903
BstDEI CTNAG 3 cut(s) 5, 109, 566
BstDSI CCRYGG 2 cut(s) 93, 314
BstF5I GGATG 2 cut(s) 664, 769
BstFNI CGCG 3 cut(s) 23, 85, 87
BstHHI GCGC 4 cut(s) 25, 87, 89, 353
BstKTI GATC 4 cut(s) 145, 805, 820, 933
BstMAI GTCTC 1 cut(s) 985
BstMBI GATC 4 cut(s) 142, 802, 817, 930
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 3 cut(s) 530, 892, 1183
BstSCI CCNGG 2 cut(s) 452, 1042
BstSFI CTRYAG 2 cut(s) 185, 555
BstUI CGCG 3 cut(s) 23, 85, 87
BstV1I GCAGC 3 cut(s) 199, 411, 698
BstV2I GAAGAC 2 cut(s) 165, 911
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
BsuRI GGCC 2 cut(s) 98, 971
BtgI CCRYGG 2 cut(s) 93, 314
BtsCI GGATG 2 cut(s) 664, 769
BtsIMutI CAGTG 1 cut(s) 152
BveI ACCTGC 2 cut(s) 204, 896
Cac8I GCNNGC 2 cut(s) 87, 903
CfoI GCGC 4 cut(s) 25, 87, 89, 353
Cfr13I GGNCC 3 cut(s) 706, 737, 1158
Csp6I GTAC 1 cut(s) 17
CspCI CAANNNNNGTGG 2 cut(s) 730, 765
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 3 cut(s) 5, 109, 566
DpnI GATC 4 cut(s) 144, 804, 819, 932
DpnII GATC 4 cut(s) 142, 802, 817, 930
DrdI GACNNNNNNGTC 2 cut(s) 713, 744
DseDI GACNNNNNNGTC 2 cut(s) 713, 744
EaeI YGGCCR 2 cut(s) 96, 969
Eam1104I CTCTTC 3 cut(s) 180, 372, 1164
EarI CTCTTC 3 cut(s) 180, 372, 1164
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 93, 314
Eco47I GGWCC 3 cut(s) 706, 737, 1158
Eco57I CTGAAG 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 452
EcoT14I CCWWGG 2 cut(s) 93, 314
EcoT22I ATGCAT 2 cut(s) 239, 894
ErhI CCWWGG 2 cut(s) 93, 314
FaqI GGGAC 2 cut(s) 800, 820
FauI CCCGC 1 cut(s) 65
FauNDI CATATG 2 cut(s) 711, 770
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 7 cut(s) 90, 99, 102, 188, 400, 687, 969
FokI GGATG 2 cut(s) 671, 776
Fsp4HI GCNGC 7 cut(s) 90, 99, 102, 188, 400, 687, 969
FspBI CTAG 7 cut(s) 155, 506, 594, 821, 902, 981, 1014
GlaI GCGC 4 cut(s) 24, 86, 88, 352
GluI GCNGC 7 cut(s) 90, 99, 102, 188, 400, 687, 969
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 684
HaeIII GGCC 2 cut(s) 98, 971
HapII CCGG 4 cut(s) 191, 852, 1044, 1058
HhaI GCGC 4 cut(s) 25, 87, 89, 353
Hin1I GRCGYC 1 cut(s) 135
Hin6I GCGC 4 cut(s) 23, 85, 87, 351
HinP1I GCGC 4 cut(s) 23, 85, 87, 351
HindIII AAGCTT 2 cut(s) 623, 1024
HinfI GANTC 7 cut(s) 117, 151, 645, 675, 848, 984, 1061
HpaII CCGG 4 cut(s) 191, 852, 1044, 1058
HphI GGTGA 6 cut(s) 216, 335, 377, 386, 821, 1163
Hpy166II GTNNAC 2 cut(s) 19, 581
Hpy188I TCNGA 1 cut(s) 924
Hpy188III TCNNGA 7 cut(s) 155, 413, 474, 701, 988, 1030, 1058
Hpy8I GTNNAC 2 cut(s) 19, 581
Hpy99I CGWCG 2 cut(s) 131, 137
HpyAV CCTTC 3 cut(s) 237, 296, 1093
HpyCH4III ACNGT 2 cut(s) 512, 559
HpyCH4IV ACGT 2 cut(s) 135, 248
HpyCH4V TGCA 8 cut(s) 187, 237, 407, 635, 681, 728, 815, 892
HpyF3I CTNAG 3 cut(s) 5, 109, 566
HpySE526I ACGT 2 cut(s) 135, 248
Hsp92I GRCGYC 1 cut(s) 135
HspAI GCGC 4 cut(s) 23, 85, 87, 351
Kpn2I TCCGGA 1 cut(s) 1057
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 142, 802, 817, 930
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 4 cut(s) 523, 703, 941, 1174
Lsp1109I GCAGC 3 cut(s) 199, 411, 698
LweI GCATC 3 cut(s) 246, 439, 802
MaeI CTAG 7 cut(s) 155, 506, 594, 821, 902, 981, 1014
MaeII ACGT 2 cut(s) 135, 248
MaeIII GTNAC 1 cut(s) 884
MalI GATC 4 cut(s) 144, 804, 819, 932
MboI GATC 4 cut(s) 142, 802, 817, 930
MboII GAAGA 5 cut(s) 167, 170, 389, 911, 1181
MfeI CAATTG 1 cut(s) 308
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 2 cut(s) 700, 1179
MluCI AATT 3 cut(s) 308, 416, 1163
MlyI GAGTC 4 cut(s) 654, 857, 993, 1070
MmeI TCCRAC 1 cut(s) 919
Mph1103I ATGCAT 2 cut(s) 239, 894
MroI TCCGGA 1 cut(s) 1057
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 2 cut(s) 245, 620
MslI CAYNNNNRTG 2 cut(s) 810, 1091
MspI CCGG 4 cut(s) 191, 852, 1044, 1058
MspR9I CCNGG 2 cut(s) 454, 1044
MunI CAATTG 1 cut(s) 308
Mva1269I GAATGC 1 cut(s) 970
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 3 cut(s) 23, 85, 87
NciI CCSGG 1 cut(s) 1044
NcoI CCATGG 2 cut(s) 93, 314
NdeI CATATG 2 cut(s) 711, 770
NdeII GATC 4 cut(s) 142, 802, 817, 930
NheI GCTAGC 1 cut(s) 901
NlaIV GGNNCC 3 cut(s) 218, 739, 1072
NmuCI GTSAC 1 cut(s) 884
NsiI ATGCAT 2 cut(s) 239, 894
NspI RCATGY 3 cut(s) 530, 892, 1183
OliI CACNNNNGTG 1 cut(s) 1091
PaqCI CACCTGC 1 cut(s) 896
PauI GCGCGC 1 cut(s) 85
PciI ACATGT 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 970
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 117, 151, 675
PflMI CCANNNNNTGG 1 cut(s) 655
PkrI GCNGC 7 cut(s) 91, 100, 103, 189, 401, 688, 970
PleI GAGTC 4 cut(s) 653, 856, 992, 1069
PpsI GAGTC 4 cut(s) 653, 856, 992, 1069
PscI ACATGT 1 cut(s) 1179
Psp1406I AACGTT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 218, 739, 1072
PspPI GGNCC 3 cut(s) 706, 737, 1158
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 1 cut(s) 817
PteI GCGCGC 1 cut(s) 85
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 810, 1091
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 245, 620
SatI GCNGC 7 cut(s) 90, 99, 102, 188, 400, 687, 969
Sau3AI GATC 4 cut(s) 142, 802, 817, 930
Sau96I GGNCC 3 cut(s) 706, 737, 1158
SchI GAGTC 4 cut(s) 654, 857, 993, 1070
ScrFI CCNGG 2 cut(s) 454, 1044
SduI GDGCHC 2 cut(s) 700, 1179
SfaNI GCATC 3 cut(s) 246, 439, 802
SfcI CTRYAG 2 cut(s) 185, 555
SinI GGWCC 3 cut(s) 706, 737, 1158
SmiMI CAYNNNNRTG 2 cut(s) 810, 1091
Sse9I AATT 3 cut(s) 308, 416, 1163
SsiI CCGC 6 cut(s) 45, 58, 90, 99, 102, 968
SspMI CTAG 7 cut(s) 155, 506, 594, 821, 902, 981, 1014
StyD4I CCNGG 2 cut(s) 452, 1042
StyI CCWWGG 2 cut(s) 93, 314
TaaI ACNGT 2 cut(s) 512, 559
TaiI ACGT 2 cut(s) 138, 251
TaqI TCGA 4 cut(s) 115, 178, 616, 1078
TasI AATT 3 cut(s) 308, 416, 1163
TatI WGTACW 1 cut(s) 16
TauI GCSGC 4 cut(s) 92, 101, 104, 971
TfiI GAWTC 3 cut(s) 117, 151, 675
Tru1I TTAA 2 cut(s) 245, 620
Tru9I TTAA 2 cut(s) 245, 620
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 3 cut(s) 187, 399, 686
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 2 cut(s) 1017, 1053
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 3 cut(s) 706, 737, 1158
XapI RAATTY 1 cut(s) 416
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 3 cut(s) 530, 892, 1183
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 7 cut(s) 155, 506, 594, 821, 902, 981, 1014
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 2 cut(s) 239, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.