RchiOBHm_Chr2g0086151

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007708
Physical Location & Seq
Reverse (-)
1297427 .. 1301582
4156 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46168

Sequence Viewer

Length: 1191 bp
ATGCTGACATGTTGTACTCCTTATTCTTTGGGACCCACAAATGAATTGGACGGCTCTGATTCCACACCTGGGTACAACAATTTCGTTGACCTCTACCCCTTCTTAGTCTTGACCAGTAGTTCGCGCCAGAGCCCAGAGGTGATGCTAAGCTCATTAGTACACGCGCCACCACTCGCTTTTCTCTCCGCCTCGCTTCCCCGCCTTTTCTCCACCCACAACGCCACGCGCGCTGCCATGGCCGCCGCCACCTCAGCTCGAATCGCAGTCGTCGGCGACGTCCATGATCAGTGGAATCTAGAAGACGACACGAAAGCTCTACGATTTCTGCAGCCAGATTTGGTTCTTTTTACAGGTGATTTTGGTGAAGAGAACATTGAACTTGTTCGCAGCGTTGCAAATCTTGAATTTGCTAAAGCAGTTATTCTGGGAAACCATGATGCCTGGCATACAAAAAAGTTTTCAGGAAAGACAAAAGACGGCGTTCAACTTCAGCTAGACTGTCTAGGCGAGGAGCATGTAGCTTACAGATGTTTGGACTTTGCTACGGTAAAACTGAGCATTGTTGGTGGACGACCTTTTTCTAGTGGGGGAAACCAGTTATTTCGATTAAAGCTTCTGTCTGCAAGGTATGGAGTCCATGACATGGATGGAAGTGCTAAGAGAATCTGCAAAGCAGCTTTGGGTGCTCCAGAGGACCATATGGTCATTATTCTTGCACACAATGGACCCACAGGTCTTGGTTCTAACTTGGATGACATATGTGGAAAAGACTGGGACTTTGGAGGTGGAGATCATGGTGATGCAGATCTAGCACAAGCCCTGTCCCTCCTAAAAGAGTCCGGCAAAACCTGTGTTCCATTGGTAGTGTTTGGTCACATGCATAAACTGCTAGCAGGTGGAAATGGTCTTCGGAAAATGATCGTGGTTGGAGTTGACAATACTATATACCTGAATGCGGCCATTGTTCCTAGAGTCAAGAGACTAAATGATGAACAAGGAACTAGCAATAAAAGCTTCATGAATAATGAACCCGGTCTTTCAACTCCGGAGTCCCGTGGCACCATTCGAGCCTTCACTTTAGTGGAGATTTTGGACGGAGGAGTAGATAAGATTGTTGAAACTTGGGTTTCTGTTGTGGGTGATAGGACCAAATTGGAAGAGGAGCACATATTATTCAAAAGTGGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

396

Amino Acids

42.74

Weight (kDa)

5.71

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 86 - 295 3.7e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 884
AasI GACNNNNNNGTC 2 cut(s) 701, 732
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 884
AccB1I GGYRCC 1 cut(s) 1058
AccB7I CCANNNNNTGG 1 cut(s) 643
AccII CGCG 4 cut(s) 124, 164, 226, 228
AccIII TCCGGA 1 cut(s) 1045
AciI CCGC 5 cut(s) 186, 199, 240, 243, 956
AcoI YGGCCR 2 cut(s) 237, 957
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 3 cut(s) 16, 74, 159
AfiI CCNNNNNNNGG 3 cut(s) 69, 643, 955
AflIII ACRYGT 1 cut(s) 8
AgsI TTSAA 6 cut(s) 377, 404, 485, 1041, 1118, 1177
AjnI CCWGG 2 cut(s) 67, 440
AleI CACNNNNGTG 1 cut(s) 1079
AluBI AGCT 8 cut(s) 150, 254, 314, 493, 521, 613, 677, 1014
AluI AGCT 8 cut(s) 150, 254, 314, 493, 521, 613, 677, 1014
Alw21I GWGCWC 2 cut(s) 688, 1167
Alw26I GTCTC 1 cut(s) 973
Aor13HI TCCGGA 1 cut(s) 1045
AoxI GGCC 2 cut(s) 237, 957
ApeKI GCWGC 4 cut(s) 230, 328, 387, 674
ApoI RAATTY 1 cut(s) 404
ArsI GACNNNNNNTTYG 4 cut(s) 103, 135, 761, 793
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 4 cut(s) 126, 166, 228, 230
AspS9I GGNCC 4 cut(s) 32, 694, 725, 1146
AsuC2I CCSGG 1 cut(s) 1032
AsuHPI GGTGA 5 cut(s) 151, 365, 374, 809, 1151
AsuNHI GCTAGC 1 cut(s) 889
AvaII GGWCC 4 cut(s) 32, 694, 725, 1146
BanI GGYRCC 1 cut(s) 1058
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 2 cut(s) 306, 899
Bbv12I GWGCWC 2 cut(s) 688, 1167
BbvCI CCTCAGC 1 cut(s) 250
BbvI GCAGC 4 cut(s) 217, 340, 399, 686
BccI CCATC 1 cut(s) 641
BceAI ACGGC 2 cut(s) 67, 493
BciT130I CCWGG 2 cut(s) 69, 442
BclI TGATCA 1 cut(s) 283
BcnI CCSGG 1 cut(s) 1032
BcoDI GTCTC 1 cut(s) 973
BfaI CTAG 8 cut(s) 296, 494, 503, 582, 809, 890, 969, 1002
BfmI CTRYAG 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 805
BisI GCNGC 7 cut(s) 231, 240, 243, 329, 388, 675, 957
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 7 cut(s) 232, 241, 244, 330, 389, 676, 958
Bme1390I CCNGG 3 cut(s) 69, 442, 1032
Bme18I GGWCC 4 cut(s) 32, 694, 725, 1146
BmgT120I GGNCC 4 cut(s) 32, 694, 725, 1146
BmiI GGNNCC 4 cut(s) 33, 34, 727, 1060
BmrFI CCNGG 3 cut(s) 69, 442, 1032
BmrI ACTGGG 1 cut(s) 781
BmsI GCATC 3 cut(s) 132, 427, 790
BmtI GCTAGC 1 cut(s) 893
BmuI ACTGGG 1 cut(s) 781
BpiI GAAGAC 2 cut(s) 306, 899
BpmI CTGGAG 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 1032
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 68, 234, 1054
BsaWI WCCGGW 1 cut(s) 1045
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 643, 955
Bse1I ACTGG 3 cut(s) 114, 595, 776
Bse8I GATNNNNATC 1 cut(s) 804
BseAI TCCGGA 1 cut(s) 1045
BseBI CCWGG 2 cut(s) 69, 442
BseDI CCNNGG 3 cut(s) 68, 234, 1054
BseGI GGATG 2 cut(s) 652, 757
BseJI GATNNNNATC 1 cut(s) 804
BseLI CCNNNNNNNGG 3 cut(s) 69, 643, 955
BseMII CTCAG 2 cut(s) 264, 545
BseNI ACTGG 3 cut(s) 114, 595, 776
BsePI GCGCGC 1 cut(s) 226
BseRI GAGGAG 3 cut(s) 524, 1113, 1175
BseXI GCAGC 4 cut(s) 217, 340, 399, 686
Bsh1236I CGCG 4 cut(s) 124, 164, 226, 228
BshFI GGCC 2 cut(s) 239, 959
BshNI GGYRCC 1 cut(s) 1058
BsiHKAI GWGCWC 2 cut(s) 688, 1167
BsiSI CCGG 3 cut(s) 840, 1032, 1046
BslFI GGGAC 4 cut(s) 45, 788, 808, 1036
BslI CCNNNNNNNGG 3 cut(s) 69, 643, 955
BsmAI GTCTC 1 cut(s) 973
BsmFI GGGAC 4 cut(s) 45, 788, 808, 1036
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 2 cut(s) 239, 959
Bsp1286I GDGCHC 3 cut(s) 134, 688, 1167
Bsp13I TCCGGA 1 cut(s) 1045
Bsp143I GATC 4 cut(s) 283, 790, 805, 918
Bsp1720I GCTNAGC 1 cut(s) 146
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 5 cut(s) 186, 199, 240, 243, 956
BspANI GGCC 2 cut(s) 239, 959
BspCNI CTCAG 2 cut(s) 263, 546
BspEI TCCGGA 1 cut(s) 1045
BspFNI CGCG 4 cut(s) 124, 164, 226, 228
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 4 cut(s) 33, 34, 727, 1060
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 884
BspOI GCTAGC 1 cut(s) 893
BspT107I GGYRCC 1 cut(s) 1058
BsrI ACTGG 3 cut(s) 114, 595, 776
BssECI CCNNGG 3 cut(s) 68, 234, 1054
BssHII GCGCGC 1 cut(s) 226
BssMI GATC 4 cut(s) 283, 790, 805, 918
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 69, 442
Bst4CI ACNGT 2 cut(s) 500, 547
Bst6I CTCTTC 2 cut(s) 360, 1152
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 886
BstC8I GCNNGC 2 cut(s) 228, 891
BstDEI CTNAG 5 cut(s) 103, 146, 250, 554, 657
BstDSI CCRYGG 2 cut(s) 234, 1054
BstF5I GGATG 2 cut(s) 652, 757
BstFNI CGCG 4 cut(s) 124, 164, 226, 228
BstHHI GCGC 4 cut(s) 126, 166, 228, 230
BstKTI GATC 4 cut(s) 286, 793, 808, 921
BstMAI GTCTC 1 cut(s) 973
BstMBI GATC 4 cut(s) 283, 790, 805, 918
BstMWI GCNNNNNNNGC 9 cut(s) 227, 236, 239, 251, 260, 683, 809, 886, 1011
BstNI CCWGG 2 cut(s) 69, 442
BstNSI RCATGY 3 cut(s) 12, 518, 880
BstSCI CCNGG 3 cut(s) 67, 440, 1030
BstSFI CTRYAG 1 cut(s) 326
BstUI CGCG 4 cut(s) 124, 164, 226, 228
BstV1I GCAGC 4 cut(s) 217, 340, 399, 686
BstV2I GAAGAC 2 cut(s) 306, 899
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 2 cut(s) 239, 959
BtgI CCRYGG 2 cut(s) 234, 1054
BtsCI GGATG 2 cut(s) 652, 757
BtsIMutI CAGTG 1 cut(s) 293
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 2 cut(s) 228, 891
CciI TCATGA 1 cut(s) 1017
CfoI GCGC 4 cut(s) 126, 166, 228, 230
Cfr13I GGNCC 4 cut(s) 32, 694, 725, 1146
Csp6I GTAC 3 cut(s) 15, 73, 158
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviQI GTAC 3 cut(s) 15, 73, 158
DdeI CTNAG 5 cut(s) 103, 146, 250, 554, 657
DpnI GATC 4 cut(s) 285, 792, 807, 920
DpnII GATC 4 cut(s) 283, 790, 805, 918
DrdI GACNNNNNNGTC 2 cut(s) 701, 732
DseDI GACNNNNNNGTC 2 cut(s) 701, 732
EaeI YGGCCR 2 cut(s) 237, 957
Eam1104I CTCTTC 2 cut(s) 360, 1152
EarI CTCTTC 2 cut(s) 360, 1152
EciI GGCGGA 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 134
Eco47I GGWCC 4 cut(s) 32, 694, 725, 1146
Eco57I CTGAAG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 67, 440
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 1 cut(s) 882
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 234
FaqI GGGAC 4 cut(s) 45, 788, 808, 1036
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 2 cut(s) 699, 758
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 7 cut(s) 231, 240, 243, 329, 388, 675, 957
FokI GGATG 2 cut(s) 659, 764
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 7 cut(s) 231, 240, 243, 329, 388, 675, 957
FspBI CTAG 8 cut(s) 296, 494, 503, 582, 809, 890, 969, 1002
GlaI GCGC 4 cut(s) 125, 165, 227, 229
GluI GCNGC 7 cut(s) 231, 240, 243, 329, 388, 675, 957
GsuI CTGGAG 1 cut(s) 672
HaeIII GGCC 2 cut(s) 239, 959
HapII CCGG 3 cut(s) 840, 1032, 1046
HhaI GCGC 4 cut(s) 126, 166, 228, 230
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 4 cut(s) 124, 164, 226, 228
HinP1I GCGC 4 cut(s) 124, 164, 226, 228
HincII GTYRAC 2 cut(s) 88, 934
HindII GTYRAC 2 cut(s) 88, 934
HindIII AAGCTT 2 cut(s) 611, 1012
HinfI GANTC 8 cut(s) 59, 258, 292, 633, 663, 836, 972, 1049
HpaII CCGG 3 cut(s) 840, 1032, 1046
HphI GGTGA 5 cut(s) 151, 365, 374, 809, 1151
Hpy166II GTNNAC 4 cut(s) 88, 160, 569, 934
Hpy188I TCNGA 2 cut(s) 58, 912
Hpy188III TCNNGA 8 cut(s) 109, 296, 401, 462, 689, 976, 1018, 1046
Hpy8I GTNNAC 4 cut(s) 88, 160, 569, 934
Hpy99I CGWCG 2 cut(s) 272, 278
HpyAV CCTTC 2 cut(s) 109, 1081
HpyCH4III ACNGT 2 cut(s) 500, 547
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 7 cut(s) 328, 395, 623, 669, 716, 803, 880
HpyF10VI GCNNNNNNNGC 9 cut(s) 227, 236, 239, 251, 260, 683, 809, 886, 1011
HpyF3I CTNAG 5 cut(s) 103, 146, 250, 554, 657
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 4 cut(s) 124, 164, 226, 228
KflI GGGWCCC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 1045
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 4 cut(s) 283, 790, 805, 918
LmnI GCTCC 3 cut(s) 511, 691, 1162
Lsp1109I GCAGC 4 cut(s) 217, 340, 399, 686
LweI GCATC 3 cut(s) 132, 427, 790
MaeI CTAG 8 cut(s) 296, 494, 503, 582, 809, 890, 969, 1002
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 872
MalI GATC 4 cut(s) 285, 792, 807, 920
MboI GATC 4 cut(s) 283, 790, 805, 918
MboII GAAGA 4 cut(s) 311, 377, 899, 1169
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 3 cut(s) 134, 688, 1167
MluCI AATT 4 cut(s) 44, 79, 404, 1151
MlyI GAGTC 4 cut(s) 642, 845, 981, 1058
MmeI TCCRAC 1 cut(s) 907
Mph1103I ATGCAT 1 cut(s) 882
MroI TCCGGA 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 1 cut(s) 608
MslI CAYNNNNRTG 2 cut(s) 798, 1079
MspI CCGG 3 cut(s) 840, 1032, 1046
MspR9I CCNGG 3 cut(s) 69, 442, 1032
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 2 cut(s) 69, 442
MvnI CGCG 4 cut(s) 124, 164, 226, 228
MwoI GCNNNNNNNGC 9 cut(s) 227, 236, 239, 251, 260, 683, 809, 886, 1011
NciI CCSGG 1 cut(s) 1032
NcoI CCATGG 1 cut(s) 234
NdeI CATATG 2 cut(s) 699, 758
NdeII GATC 4 cut(s) 283, 790, 805, 918
NheI GCTAGC 1 cut(s) 889
NlaIV GGNNCC 4 cut(s) 33, 34, 727, 1060
NmuCI GTSAC 1 cut(s) 872
NsiI ATGCAT 1 cut(s) 882
NspI RCATGY 3 cut(s) 12, 518, 880
OliI CACNNNNGTG 1 cut(s) 1079
PagI TCATGA 1 cut(s) 1017
PaqCI CACCTGC 1 cut(s) 884
PauI GCGCGC 1 cut(s) 226
PciI ACATGT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 4 cut(s) 59, 258, 292, 663
PflMI CCANNNNNTGG 1 cut(s) 643
PkrI GCNGC 7 cut(s) 232, 241, 244, 330, 389, 676, 958
PleI GAGTC 4 cut(s) 641, 844, 980, 1057
PpsI GAGTC 4 cut(s) 641, 844, 980, 1057
PpuMI RGGWCCY 1 cut(s) 32
PscI ACATGT 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 67, 440
PspGI CCWGG 2 cut(s) 67, 440
PspN4I GGNNCC 4 cut(s) 33, 34, 727, 1060
PspPI GGNCC 4 cut(s) 32, 694, 725, 1146
PspPPI RGGWCCY 1 cut(s) 32
PstI CTGCAG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 805
PteI GCGCGC 1 cut(s) 226
RsaI GTAC 3 cut(s) 16, 74, 159
RsaNI GTAC 3 cut(s) 15, 73, 158
RseI CAYNNNNRTG 2 cut(s) 798, 1079
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 7 cut(s) 231, 240, 243, 329, 388, 675, 957
Sau3AI GATC 4 cut(s) 283, 790, 805, 918
Sau96I GGNCC 4 cut(s) 32, 694, 725, 1146
SchI GAGTC 4 cut(s) 642, 845, 981, 1058
ScrFI CCNGG 3 cut(s) 69, 442, 1032
SduI GDGCHC 3 cut(s) 134, 688, 1167
SfaNI GCATC 3 cut(s) 132, 427, 790
SfcI CTRYAG 1 cut(s) 326
SinI GGWCC 4 cut(s) 32, 694, 725, 1146
SmiMI CAYNNNNRTG 2 cut(s) 798, 1079
Sse9I AATT 4 cut(s) 44, 79, 404, 1151
SsiI CCGC 5 cut(s) 186, 199, 240, 243, 956
SspMI CTAG 8 cut(s) 296, 494, 503, 582, 809, 890, 969, 1002
StyD4I CCNGG 3 cut(s) 67, 440, 1030
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 500, 547
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 256, 604, 1066
TasI AATT 4 cut(s) 44, 79, 404, 1151
TatI WGTACW 2 cut(s) 14, 157
TauI GCSGC 3 cut(s) 242, 245, 959
TfiI GAWTC 4 cut(s) 59, 258, 292, 663
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 4 cut(s) 230, 328, 387, 674
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 5 cut(s) 57, 1005, 1006, 1034, 1041
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 643
VpaK11BI GGWCC 4 cut(s) 32, 694, 725, 1146
XapI RAATTY 1 cut(s) 404
XbaI TCTAGA 1 cut(s) 295
XceI RCATGY 3 cut(s) 12, 518, 880
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 644
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 8 cut(s) 296, 494, 503, 582, 809, 890, 969, 1002
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.