pycom02g00990

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007708
Physical Location & Seq
Reverse (-)
578302 .. 580965
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00990.7

Sequence Viewer

Length: 1005 bp
ATGCCACTCACCAACCCCGTGTTTCATCTCTGTTTCGCCTCCCCCCACCCTCGTTCCGACCACATCGCCGCGCGCTCTTCCATGGCTTCCTCCGTAGCTCGGAGCTCAGCTCGTATAGCCGTTGTCGGTGACGTCCATGATCAATGGAATCTAGAAGAAGATAGGAAAGCACTTCAACTGCTGCAGCCGGATTTGGTTCTTTTTACAGGTGATTTTGGTGAAGAGAATGTTGAACTCGTTAAGAGCGTTGCAGATCTCGATCTTGCTAAAGCAATTATTTTAGGAAACCATGACGCCTGGCATACAACACAGTTTTCTCGAAAGAAAAAGGACAGCGTTCAACTTCAGCTAGAATGTCTCGGTGAGGAGCATGTGGGTTATCGGCGTTTGGACTATCCTGCTGTGAAGCTGAGCGTTGTTGGTGGGCGACCTTTTTCTTGTGGGGGAAATGAGTTTTTTCGACAAAAGCTTCTGTCTGCAAGGTATGGGGTCCAAGACATGGAAGGAAGTGCAAAGAGAATATGCGAAGCAACTTTGGGTGCTCCTGAGGACCATTTGATCATATTTCTTGCACATAATGGACCCACAGGTCTTGGGTCTAACGTGGATGACATATGTGGAAAAGATTGGGTGTTTGGAGCTGGGGATCATGGTGACCCAGATCTTGCACAAGCCCTGTCCCTCTTAAAAGAGACTGCAACAATCTGTGCTCCACTGGTGGTGTTTGGTCACATGCATAAAGAGCTAGCGCATGGAAATGGTCTTCGGAAAATGATTGTGGTTGGAGACGACAACACTATATACCTCAATGGTGCTATTGTTCCTAGAGTTAAGAGACTGAACGTTGAGCAAGGAACACCGGAGTCCTGTGGTACCGTGCGCGCCTTCACTTTAGTGGAGATCTTGGACGGAACAGTTGATAAAATCGTTGAAAGTTGGGTTTCAGTTGCCGGCGATAGGATCACATTGGAAGAGGAGCACATTTTGTTCAAAAGTGGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

335

Amino Acids

36.39

Weight (kDa)

5.59

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 588
AatII GACGTC 1 cut(s) 135
Acc65I GGTACC 1 cut(s) 872
AccB1I GGYRCC 1 cut(s) 872
AccB7I CCANNNNNTGG 1 cut(s) 499
AccII CGCG 3 cut(s) 71, 73, 882
AciI CCGC 2 cut(s) 69, 999
AclI AACGTT 1 cut(s) 843
AclWI GGATC 2 cut(s) 654, 968
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 2 cut(s) 132, 294
AfaI GTAC 1 cut(s) 874
AfiI CCNNNNNNNGG 3 cut(s) 99, 499, 957
AgsI TTSAA 5 cut(s) 176, 233, 341, 932, 991
AjnI CCWGG 1 cut(s) 296
AleI CACNNNNGTG 1 cut(s) 893
AluBI AGCT 8 cut(s) 98, 105, 110, 349, 409, 469, 641, 745
AluI AGCT 8 cut(s) 98, 105, 110, 349, 409, 469, 641, 745
Alw21I GWGCWC 4 cut(s) 107, 544, 712, 981
Alw26I GTCTC 4 cut(s) 362, 686, 780, 829
AlwI GGATC 2 cut(s) 654, 968
ApeKI GCWGC 2 cut(s) 181, 184
Asp718I GGTACC 1 cut(s) 872
AspLEI GCGC 5 cut(s) 73, 75, 751, 882, 884
AspS9I GGNCC 3 cut(s) 490, 550, 581
AsuHPI GGTGA 5 cut(s) 140, 221, 230, 374, 665
AsuNHI GCTAGC 1 cut(s) 745
AvaII GGWCC 3 cut(s) 490, 550, 581
AxyI CCTNAGG 1 cut(s) 546
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 4 cut(s) 107, 544, 712, 981
BbvI GCAGC 2 cut(s) 168, 196
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 298
BclI TGATCA 2 cut(s) 139, 558
BcoDI GTCTC 4 cut(s) 362, 686, 780, 829
BfaI CTAG 4 cut(s) 152, 350, 746, 825
BfmI CTRYAG 1 cut(s) 182
BglII AGATCT 3 cut(s) 253, 661, 900
BisI GCNGC 3 cut(s) 69, 182, 185
BlpI GCTNAGC 2 cut(s) 106, 410
BlsI GCNGC 3 cut(s) 70, 183, 186
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 3 cut(s) 490, 550, 581
BmgT120I GGNCC 3 cut(s) 490, 550, 581
BmiI GGNNCC 3 cut(s) 491, 583, 874
BmrFI CCNGG 1 cut(s) 298
BmtI GCTAGC 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 94, 126
Bpu1102I GCTNAGC 2 cut(s) 106, 410
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 2 cut(s) 132, 294
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 1 cut(s) 859
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 499, 957
Bse118I RCCGGY 1 cut(s) 950
Bse1I ACTGG 1 cut(s) 720
Bse21I CCTNAGG 1 cut(s) 546
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 613
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 99, 499, 957
BseMII CTCAG 3 cut(s) 120, 401, 537
BseNI ACTGG 1 cut(s) 720
BsePI GCGCGC 2 cut(s) 71, 880
BseRI GAGGAG 2 cut(s) 380, 989
BseXI GCAGC 2 cut(s) 168, 196
BseYI CCCAGC 1 cut(s) 641
Bsh1236I CGCG 3 cut(s) 71, 73, 882
BshNI GGYRCC 1 cut(s) 872
BsiHKAI GWGCWC 4 cut(s) 107, 544, 712, 981
BsiSI CCGG 3 cut(s) 188, 860, 951
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 3 cut(s) 99, 499, 957
BsmAI GTCTC 4 cut(s) 362, 686, 780, 829
BsmBI CGTCTC 1 cut(s) 780
BsmFI GGGAC 1 cut(s) 664
Bsp1286I GDGCHC 4 cut(s) 107, 544, 712, 981
Bsp143I GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
Bsp1720I GCTNAGC 2 cut(s) 106, 410
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 2 cut(s) 69, 999
BspCNI CTCAG 3 cut(s) 119, 402, 538
BspFNI CGCG 3 cut(s) 71, 73, 882
BspLI GGNNCC 3 cut(s) 491, 583, 874
BspMAI CTGCAG 1 cut(s) 186
BspOI GCTAGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 654, 968
BspQI GCTCTTC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 872
BsrFI RCCGGY 1 cut(s) 950
BsrI ACTGG 1 cut(s) 720
BssAI RCCGGY 1 cut(s) 950
BssECI CCNNGG 1 cut(s) 81
BssHII GCGCGC 2 cut(s) 71, 880
BssMI GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
BssNI GRCGYC 2 cut(s) 132, 294
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 3 cut(s) 312, 877, 916
Bst6I CTCTTC 3 cut(s) 82, 216, 966
BstACI GRCGYC 2 cut(s) 132, 294
BstC8I GCNNGC 4 cut(s) 73, 747, 882, 952
BstDEI CTNAG 3 cut(s) 106, 410, 546
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 3 cut(s) 71, 73, 882
BstHHI GCGC 5 cut(s) 73, 75, 751, 882, 884
BstKTI GATC 8 cut(s) 142, 256, 262, 561, 649, 664, 903, 963
BstMAI GTCTC 4 cut(s) 362, 686, 780, 829
BstMBI GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
BstMWI GCNNNNNNNGC 2 cut(s) 116, 742
BstNI CCWGG 1 cut(s) 298
BstNSI RCATGY 2 cut(s) 374, 736
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 182
BstUI CGCG 3 cut(s) 71, 73, 882
BstV1I GCAGC 2 cut(s) 168, 196
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 3 cut(s) 253, 661, 900
BstYI RGATCY 3 cut(s) 253, 661, 900
Bsu36I CCTNAGG 1 cut(s) 546
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 1 cut(s) 713
Cac8I GCNNGC 4 cut(s) 73, 747, 882, 952
CfoI GCGC 5 cut(s) 73, 75, 751, 882, 884
Cfr10I RCCGGY 1 cut(s) 950
Cfr13I GGNCC 3 cut(s) 490, 550, 581
CseI GACGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 873
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 8 cut(s) 82, 137, 290, 371, 499, 650, 733, 752
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 3 cut(s) 106, 410, 546
DpnI GATC 8 cut(s) 141, 255, 261, 560, 648, 663, 902, 962
DpnII GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
DrdI GACNNNNNNGTC 1 cut(s) 588
DseDI GACNNNNNNGTC 1 cut(s) 588
Eam1104I CTCTTC 3 cut(s) 82, 216, 966
EarI CTCTTC 3 cut(s) 82, 216, 966
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 3 cut(s) 490, 550, 581
Eco53kI GAGCTC 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 105
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 738
EcoT38I GRGCYC 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 780
FaeI CATG 8 cut(s) 85, 140, 293, 374, 502, 653, 736, 755
FaqI GGGAC 1 cut(s) 664
FatI CATG 8 cut(s) 81, 136, 289, 370, 498, 649, 732, 751
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 2 cut(s) 139, 558
Fnu4HI GCNGC 3 cut(s) 69, 182, 185
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 3 cut(s) 69, 182, 185
FspBI CTAG 4 cut(s) 152, 350, 746, 825
GlaI GCGC 5 cut(s) 72, 74, 750, 881, 883
GluI GCNGC 3 cut(s) 69, 182, 185
GsaI CCCAGC 1 cut(s) 645
HapII CCGG 3 cut(s) 188, 860, 951
HgaI GACGC 1 cut(s) 302
HhaI GCGC 5 cut(s) 73, 75, 751, 882, 884
Hin1I GRCGYC 2 cut(s) 132, 294
Hin1II CATG 8 cut(s) 85, 140, 293, 374, 502, 653, 736, 755
Hin6I GCGC 5 cut(s) 71, 73, 749, 880, 882
HinP1I GCGC 5 cut(s) 71, 73, 749, 880, 882
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 2 cut(s) 148, 863
HpaII CCGG 3 cut(s) 188, 860, 951
HphI GGTGA 5 cut(s) 140, 221, 230, 374, 665
Hpy188I TCNGA 3 cut(s) 58, 102, 768
Hpy188III TCNNGA 4 cut(s) 152, 257, 318, 545
HpyAV CCTTC 2 cut(s) 497, 895
HpyCH4III ACNGT 3 cut(s) 312, 877, 916
HpyCH4IV ACGT 3 cut(s) 132, 603, 843
HpyCH4V TGCA 8 cut(s) 184, 251, 479, 512, 572, 668, 698, 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 742
HpyF3I CTNAG 3 cut(s) 106, 410, 546
HpySE526I ACGT 3 cut(s) 132, 603, 843
Hsp92I GRCGYC 2 cut(s) 132, 294
Hsp92II CATG 8 cut(s) 85, 140, 293, 374, 502, 653, 736, 755
HspAI GCGC 5 cut(s) 71, 73, 749, 880, 882
KpnI GGTACC 1 cut(s) 876
KroI GCCGGC 1 cut(s) 950
KroNI GCCGGC 1 cut(s) 952
Ksp22I TGATCA 2 cut(s) 139, 558
Kzo9I GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 6 cut(s) 102, 367, 547, 638, 715, 976
Lsp1109I GCAGC 2 cut(s) 168, 196
MaeI CTAG 4 cut(s) 152, 350, 746, 825
MaeII ACGT 3 cut(s) 132, 603, 843
MaeIII GTNAC 3 cut(s) 128, 653, 728
MalI GATC 8 cut(s) 141, 255, 261, 560, 648, 663, 902, 962
MboI GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
MboII GAAGA 6 cut(s) 69, 167, 170, 233, 755, 983
MflI RGATCY 3 cut(s) 253, 661, 900
MhlI GDGCHC 4 cut(s) 107, 544, 712, 981
MluCI AATT 1 cut(s) 273
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 2 cut(s) 81, 763
MnlI CCTC 8 cut(s) 49, 60, 100, 358, 541, 692, 815, 967
Mph1103I ATGCAT 1 cut(s) 738
MroNI GCCGGC 1 cut(s) 950
MseI TTAA 3 cut(s) 240, 686, 831
MslI CAYNNNNRTG 2 cut(s) 756, 893
MspI CCGG 3 cut(s) 188, 860, 951
MspR9I CCNGG 1 cut(s) 298
MvaI CCWGG 1 cut(s) 298
MvnI CGCG 3 cut(s) 71, 73, 882
MwoI GCNNNNNNNGC 2 cut(s) 116, 742
NaeI GCCGGC 1 cut(s) 952
NcoI CCATGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 614
NdeII GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
NgoMIV GCCGGC 1 cut(s) 950
NheI GCTAGC 1 cut(s) 745
NlaIII CATG 8 cut(s) 85, 140, 293, 374, 502, 653, 736, 755
NlaIV GGNNCC 3 cut(s) 491, 583, 874
NmuCI GTSAC 3 cut(s) 128, 653, 728
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 2 cut(s) 374, 736
OliI CACNNNNGTG 1 cut(s) 893
PauI GCGCGC 2 cut(s) 71, 880
PciSI GCTCTTC 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdiI GCCGGC 1 cut(s) 952
PfeI GAWTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 3 cut(s) 70, 183, 186
PleI GAGTC 1 cut(s) 871
PpsI GAGTC 1 cut(s) 871
Psp124BI GAGCTC 1 cut(s) 107
Psp1406I AACGTT 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 296
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 3 cut(s) 491, 583, 874
PspPI GGNCC 3 cut(s) 490, 550, 581
PstI CTGCAG 1 cut(s) 186
PsuI RGATCY 3 cut(s) 253, 661, 900
PteI GCGCGC 2 cut(s) 71, 880
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
RseI CAYNNNNRTG 2 cut(s) 756, 893
SacI GAGCTC 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 3 cut(s) 240, 686, 831
SatI GCNGC 3 cut(s) 69, 182, 185
Sau3AI GATC 8 cut(s) 139, 253, 259, 558, 646, 661, 900, 960
Sau96I GGNCC 3 cut(s) 490, 550, 581
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 4 cut(s) 107, 544, 712, 981
SfcI CTRYAG 1 cut(s) 182
SinI GGWCC 3 cut(s) 490, 550, 581
SmiMI CAYNNNNRTG 2 cut(s) 756, 893
Sse9I AATT 1 cut(s) 273
SsiI CCGC 2 cut(s) 69, 999
SspMI CTAG 4 cut(s) 152, 350, 746, 825
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 296
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 3 cut(s) 312, 877, 916
TaiI ACGT 3 cut(s) 135, 606, 846
TaqI TCGA 3 cut(s) 258, 319, 460
TasI AATT 1 cut(s) 273
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 3 cut(s) 240, 686, 831
Tru9I TTAA 3 cut(s) 240, 686, 831
TscAI CASTG 1 cut(s) 720
TseFI GTSAC 3 cut(s) 128, 653, 728
TseI GCWGC 2 cut(s) 181, 184
Tsp45I GTSAC 3 cut(s) 128, 653, 728
TspDTI ATGAA 1 cut(s) 14
TspGWI ACGGA 2 cut(s) 82, 924
TspRI CASTG 1 cut(s) 720
Van91I CCANNNNNTGG 1 cut(s) 499
VpaK11BI GGWCC 3 cut(s) 490, 550, 581
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 2 cut(s) 374, 736
XspI CTAG 4 cut(s) 152, 350, 746, 825
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.