MD02G1012200.v1.1

Ribosomal protein S6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008708
Physical Location & Seq
Reverse (-)
770776 .. 772957
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1012200.v1.1.491

Sequence Viewer

Length: 450 bp
ATGCCGCTGTACGATTGTATGCTGCTGCTGAAGCCCCATGTGAGGAAGGAGTCTCTGATGGACTTGGTTGCTCGAGTGAGCAAACACGTTTGCAGAAGAAATGGAGTTATTACTGACATGAAGTCGTTCGGCACTGTTCAGCTCGGTTATGGTATTAAGAAGCTTGATGGAAGGTATTATCAGGGCCAACTAATGCAAATGACAATGATGACTACACCCAACATCAACAAGGAGCTGCACTACTTGAACAAGGAAGATCGGTTGCTGCGCTGGCTGTTGGTTAAACACCGAGAAACAAAGTACGGACAGGAATTTCTAAGTGAAGAGGATTCAAAAAGTGAATTCAATAAGCTTCAGCGGAGCAGCATATATAATATGGATGAGTCCGAGACTGAGGATGATGACGACGATGACGAGGAATATGATGTGAAATATGACGTGGAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S6

Protein Analysis

150

Amino Acids

17.69

Weight (kDa)

5.14

Isoelectric Point (pI)

57.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 4 - 95 1.5e-15 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 358
AcsI RAATTY 2 cut(s) 311, 341
AcuI CTGAAG 2 cut(s) 50, 338
AfaI GTAC 2 cut(s) 11, 302
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 3 cut(s) 247, 333, 346
AhdI GACNNNNNGTC 1 cut(s) 121
AjiI CACGTC 1 cut(s) 439
AluBI AGCT 4 cut(s) 142, 163, 235, 352
AluI AGCT 4 cut(s) 142, 163, 235, 352
Alw26I GTCTC 2 cut(s) 57, 383
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 5 cut(s) 22, 25, 235, 265, 363
ApoI RAATTY 2 cut(s) 311, 341
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 72
BbvI GCAGC 5 cut(s) 9, 12, 222, 252, 375
BccI CCATC 2 cut(s) 52, 161
BcoDI GTCTC 2 cut(s) 57, 383
BisI GCNGC 6 cut(s) 5, 23, 26, 236, 266, 364
BlsI GCNGC 6 cut(s) 6, 24, 27, 237, 267, 365
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 42
BseGI GGATG 2 cut(s) 385, 403
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 384
BseXI GCAGC 5 cut(s) 9, 12, 222, 252, 375
BsgI GTGCAG 1 cut(s) 221
BshFI GGCC 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 2 cut(s) 57, 383
BsnI GGCC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 72
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 2 cut(s) 5, 358
BspANI GGCC 1 cut(s) 186
BspCNI CTCAG 1 cut(s) 385
BssMI GATC 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 2 cut(s) 317, 393
BstF5I GGATG 2 cut(s) 385, 403
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 2 cut(s) 57, 383
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 31, 271
BstV1I GCAGC 5 cut(s) 9, 12, 222, 252, 375
BsuRI GGCC 1 cut(s) 186
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 385, 403
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 272
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 10, 301
CviAII CATG 2 cut(s) 38, 118
CviJI RGCY 7 cut(s) 34, 142, 163, 186, 235, 274, 352
CviKI_1 RGCY 7 cut(s) 34, 142, 163, 186, 235, 274, 352
CviQI GTAC 2 cut(s) 10, 301
DdeI CTNAG 2 cut(s) 317, 393
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
DriI GACNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 318
Eco57I CTGAAG 2 cut(s) 50, 338
Eco88I CYCGRG 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 341
FaeI CATG 2 cut(s) 41, 121
FatI CATG 2 cut(s) 37, 117
Fnu4HI GCNGC 6 cut(s) 5, 23, 26, 236, 266, 364
FokI GGATG 2 cut(s) 392, 410
Fsp4HI GCNGC 6 cut(s) 5, 23, 26, 236, 266, 364
GlaI GCGC 1 cut(s) 269
GluI GCNGC 6 cut(s) 5, 23, 26, 236, 266, 364
HaeIII GGCC 1 cut(s) 186
HhaI GCGC 1 cut(s) 270
Hin1II CATG 2 cut(s) 41, 121
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 161, 350
HinfI GANTC 3 cut(s) 50, 329, 383
Hpy188I TCNGA 2 cut(s) 57, 388
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 3 cut(s) 40, 165, 437
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 87, 438
HpyCH4V TGCA 3 cut(s) 93, 196, 238
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 271
HpyF3I CTNAG 2 cut(s) 317, 393
HpySE526I ACGT 2 cut(s) 87, 438
Hsp92II CATG 2 cut(s) 41, 121
HspAI GCGC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 2 cut(s) 232, 360
LpnPI CCDG 3 cut(s) 167, 256, 293
Lsp1109I GCAGC 5 cut(s) 9, 12, 222, 252, 375
MaeII ACGT 2 cut(s) 87, 438
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 3 cut(s) 108, 266, 335
MluCI AATT 2 cut(s) 311, 341
MlyI GAGTC 2 cut(s) 59, 392
MnlI CCTC 4 cut(s) 36, 319, 388, 409
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 2 cut(s) 156, 282
MspA1I CMGCKG 2 cut(s) 7, 358
MwoI GCNNNNNNNGC 2 cut(s) 31, 271
NdeII GATC 1 cut(s) 256
NlaIII CATG 2 cut(s) 41, 121
PaeR7I CTCGAG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 329
PkrI GCNGC 6 cut(s) 6, 24, 27, 237, 267, 365
PleI GAGTC 2 cut(s) 58, 391
PpsI GAGTC 2 cut(s) 58, 391
PspPI GGNCC 1 cut(s) 184
PspXI VCTCGAGB 1 cut(s) 72
RsaI GTAC 2 cut(s) 11, 302
RsaNI GTAC 2 cut(s) 10, 301
SaqAI TTAA 2 cut(s) 156, 282
SatI GCNGC 6 cut(s) 5, 23, 26, 236, 266, 364
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 2 cut(s) 59, 392
SetI ASST 7 cut(s) 90, 144, 165, 176, 237, 354, 441
Sfr274I CTCGAG 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 2 cut(s) 311, 341
SsiI CCGC 2 cut(s) 5, 358
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 90, 441
TaqI TCGA 1 cut(s) 73
TasI AATT 2 cut(s) 311, 341
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 2 cut(s) 156, 282
Tru9I TTAA 2 cut(s) 156, 282
TscAI CASTG 1 cut(s) 139
TseI GCWGC 5 cut(s) 22, 25, 235, 265, 363
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 1 cut(s) 139
XapI RAATTY 2 cut(s) 311, 341
XhoI CTCGAG 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.