MD15G1158000.v1.1

Ribosomal protein S6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008708
Physical Location & Seq
Reverse (-)
11739415 .. 11741733
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1158000.v1.1.491

Sequence Viewer

Length: 465 bp
ATGCCGCTGTACGATTGTATGCTGCTGCTGAAACCCCATGTGAGGAAGGAGTCTCTGATGGACTTGGTCGCTCGAGTGAGCAAACACGTTTACAGAAGAAATGGAGTTATTACTGACATGAAGTCATTCGGCACTGTTCAGCTGGGTTATGGTATTAAAAAGCTTGATGGAAGGTATTATCAGGTTGTAGGTCAACTAATGCAAATGACGATGATGTCTACGCCCAACATCAACAAGGAGCTGCACTACTTGAACAAGGAAGATCGGTTGCTGCGCTGGCTGTTGGTTAAACACCGAGAAACAAAGTATGGACAGGAATTTCTTAGTGAAGAGGATTCGAAAAGTGAATTTAATAAGCTTCAGCGGAGCAGCATATACGGTATGAATGAAGATGAGGACGAGGACGAGGATGATGATGACGATGACGAGGAATACGATGTGGAACAAGAAGAAAAACGTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S6

Protein Analysis

155

Amino Acids

18.37

Weight (kDa)

4.91

Isoelectric Point (pI)

61.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 4 - 97 1.3e-14 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 218
AciI CCGC 2 cut(s) 5, 364
AcsI RAATTY 2 cut(s) 317, 347
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 2 cut(s) 11, 460
AfiI CCNNNNNNNGG 1 cut(s) 42
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 1 cut(s) 253
AhdI GACNNNNNGTC 1 cut(s) 121
AluBI AGCT 4 cut(s) 142, 163, 241, 358
AluI AGCT 4 cut(s) 142, 163, 241, 358
Alw26I GTCTC 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 72
ApeKI GCWGC 5 cut(s) 22, 25, 241, 271, 369
ApoI RAATTY 2 cut(s) 317, 347
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 276
AsuII TTCGAA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 72
BbvI GCAGC 5 cut(s) 9, 12, 228, 258, 381
BccI CCATC 2 cut(s) 52, 161
BcoDI GTCTC 1 cut(s) 57
BisI GCNGC 6 cut(s) 5, 23, 26, 242, 272, 370
BlsI GCNGC 6 cut(s) 6, 24, 27, 243, 273, 371
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 338
Bsc4I CCNNNNNNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 42
BseXI GCAGC 5 cut(s) 9, 12, 228, 258, 381
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 227
BsiHKCI CYCGRG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 338
Bsp143I GATC 1 cut(s) 262
BspACI CCGC 2 cut(s) 5, 364
BspT104I TTCGAA 1 cut(s) 338
BssMI GATC 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 136, 380
Bst6I CTCTTC 1 cut(s) 324
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstV1I GCAGC 5 cut(s) 9, 12, 228, 258, 381
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 278
CfoI GCGC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 10, 459
CviAII CATG 2 cut(s) 38, 118
CviJI RGCY 5 cut(s) 142, 163, 241, 280, 358
CviKI_1 RGCY 5 cut(s) 142, 163, 241, 280, 358
CviQI GTAC 2 cut(s) 10, 459
DdeI CTNAG 1 cut(s) 323
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
DrdI GACNNNNNNGTC 1 cut(s) 214
DriI GACNNNNNGTC 1 cut(s) 121
DseDI GACNNNNNNGTC 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 324
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 72
FaeI CATG 2 cut(s) 41, 121
FaiI YATR 8 cut(s) 20, 39, 119, 150, 309, 374, 376, 383
FatI CATG 2 cut(s) 37, 117
FblI GTMKAC 1 cut(s) 218
Fnu4HI GCNGC 6 cut(s) 5, 23, 26, 242, 272, 370
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 6 cut(s) 5, 23, 26, 242, 272, 370
GlaI GCGC 1 cut(s) 275
GluI GCNGC 6 cut(s) 5, 23, 26, 242, 272, 370
GsaI CCCAGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 41, 121
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 194
HindII GTYRAC 1 cut(s) 194
HindIII AAGCTT 2 cut(s) 161, 356
HinfI GANTC 2 cut(s) 50, 335
Hpy166II GTNNAC 3 cut(s) 91, 194, 219
Hpy188I TCNGA 1 cut(s) 57
Hpy8I GTNNAC 3 cut(s) 91, 194, 219
HpyAV CCTTC 2 cut(s) 40, 165
HpyCH4III ACNGT 2 cut(s) 136, 380
HpyCH4IV ACGT 2 cut(s) 87, 457
HpyCH4V TGCA 2 cut(s) 202, 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 2 cut(s) 87, 457
Hsp92II CATG 2 cut(s) 41, 121
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 238, 366
LpnPI CCDG 4 cut(s) 128, 167, 262, 299
Lsp1109I GCAGC 5 cut(s) 9, 12, 228, 258, 381
MaeII ACGT 2 cut(s) 87, 457
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 5 cut(s) 108, 272, 341, 401, 461
MluCI AATT 2 cut(s) 317, 347
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 6 cut(s) 36, 325, 388, 394, 400, 421
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 156, 288, 351
MspA1I CMGCKG 3 cut(s) 7, 142, 364
MwoI GCNNNNNNNGC 1 cut(s) 277
NdeII GATC 1 cut(s) 262
NlaIII CATG 2 cut(s) 41, 121
NspV TTCGAA 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 65
PkrI GCNGC 6 cut(s) 6, 24, 27, 243, 273, 371
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 142
PspXI VCTCGAGB 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 142
RsaI GTAC 2 cut(s) 11, 460
RsaNI GTAC 2 cut(s) 10, 459
SaqAI TTAA 3 cut(s) 156, 288, 351
SatI GCNGC 6 cut(s) 5, 23, 26, 242, 272, 370
Sau3AI GATC 1 cut(s) 262
SchI GAGTC 1 cut(s) 59
Sfr274I CTCGAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 2 cut(s) 317, 347
SsiI CCGC 2 cut(s) 5, 364
TaaI ACNGT 2 cut(s) 136, 380
TaiI ACGT 2 cut(s) 90, 460
TaqI TCGA 2 cut(s) 73, 338
TasI AATT 2 cut(s) 317, 347
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 3 cut(s) 156, 288, 351
Tru9I TTAA 3 cut(s) 156, 288, 351
TscAI CASTG 1 cut(s) 139
TseI GCWGC 5 cut(s) 22, 25, 241, 271, 369
TspDTI ATGAA 3 cut(s) 134, 398, 402
TspRI CASTG 1 cut(s) 139
Tth111I GACNNNGTC 1 cut(s) 65
XapI RAATTY 2 cut(s) 317, 347
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.