MD02G1012800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012962
Physical Location & Seq
Forward (+)
806892 .. 807857
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1012800.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGCCGCTGCAGGAGCTGCCGCGGACGGGCTGTTCCGGTGCGTGTACGAGGGTTGCTTATCAGAGTTGGATATCGGAGTGGAGCGGCGCCCGTACCACCGCAACTGTGGCTGTGCCCTACACAACAAGTCACCTAACAAGCAATGTACCCACGGAGGGCCTAAGAGTAAGAAAGTGTCGTATCCGCTAAGGCGGGCTTGGAGCGAAGGGAACTTGGCTTTGGTGGCTTCAGCCGGTTCTTCTGTGCACTCCTCGCCGTCTTCCTCGCCGGCTAAGCACCACCATGCGGTCGGGAGGCTACATCAGCGGCGGCAGTCGCATCAACAATTGGATACTTTGTGCGATGAGGACGAGGAGGAGGATGAGGAGGAGAATGTTCTCTTCAAGGTTTTAGACTGCAAATATGAAATCTTGGATCCCCTTCTTCCTTGCTCTCCTTCAAAACCCAATTTGGGGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.76

Weight (kDa)

6.49

Isoelectric Point (pI)

71.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46490 AT5G35110
fragaria_vesca FvH4_1g01610
malus_domestica MD02G1012800.v1.1 MD15G1158600.v1.1
prunus_persica Prupe.7G253800_v2.0.a1
pyrus_communis pycom15g14230
rosa_chinensis RchiOBHm_Chr2g0086561
rosa_laevigata RLG00000015767
rosa_multiflora Rmu_co8199806.1_g000001 Rmu_ssc0000449.1_g000004
rosa_roxburghii Rroxscaffold_2G00154440
rosa_rugosa Rorug01G0467900
rosa_samantha Rh2AG018900 Rh2BG018500 Rh2CG019400 Rh2DG020200
rosa_wichuraiana Rw2G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 1 cut(s) 23
AclWI GGATC 2 cut(s) 410, 423
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 88
AfaI GTAC 3 cut(s) 47, 95, 148
AfiI CCNNNNNNNGG 5 cut(s) 27, 156, 286, 452, 453
AgsI TTSAA 2 cut(s) 385, 441
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw21I GWGCWC 1 cut(s) 249
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 2 cut(s) 410, 423
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 2 cut(s) 3, 158
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 8, 17
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 123
BaeGI GKGCMC 2 cut(s) 118, 249
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 241
BciVI GTATCC 2 cut(s) 192, 325
BfaI CTAG 1 cut(s) 460
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 91
BfuI GTATCC 2 cut(s) 192, 325
BisI GCNGC 7 cut(s) 6, 9, 18, 21, 86, 308, 311
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 7 cut(s) 7, 10, 19, 22, 87, 309, 312
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 89, 417
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 21, 151
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 156, 286, 452, 453
Bse118I RCCGGY 2 cut(s) 233, 268
Bse3DI GCAATG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 21, 151
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 5 cut(s) 27, 156, 286, 452, 453
BseMI GCAATG 1 cut(s) 149
BseRI GAGGAG 5 cut(s) 241, 368, 371, 380, 383
BseSI GKGCMC 2 cut(s) 118, 249
BseXI GCAGC 1 cut(s) 4
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 2 cut(s) 5, 160
BshNI GGYRCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 3 cut(s) 37, 234, 269
BslI CCNNNNNNNGG 5 cut(s) 27, 156, 286, 452, 453
BsnI GGCC 2 cut(s) 5, 160
Bsp1286I GDGCHC 2 cut(s) 118, 249
Bsp143I GATC 2 cut(s) 415, 456
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 2 cut(s) 5, 160
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 89, 417
BspMAI CTGCAG 1 cut(s) 13
BspPI GGATC 2 cut(s) 410, 423
BspT107I GGYRCC 1 cut(s) 87
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 2 cut(s) 233, 268
BssAI RCCGGY 2 cut(s) 233, 268
BssECI CCNNGG 2 cut(s) 21, 151
BssMI GATC 2 cut(s) 415, 456
BssNI GRCGYC 1 cut(s) 88
Bst4CI ACNGT 1 cut(s) 107
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 195, 270
BstDEI CTNAG 3 cut(s) 162, 188, 273
BstDSI CCRYGG 2 cut(s) 21, 151
BstF5I GGATG 1 cut(s) 367
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 2 cut(s) 418, 459
BstMBI GATC 2 cut(s) 415, 456
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 8 cut(s) 14, 17, 108, 224, 253, 274, 304, 316
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 118, 249
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 4
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 2 cut(s) 415, 456
BstYI RGATCY 2 cut(s) 415, 456
BsuI GTATCC 2 cut(s) 192, 325
BsuRI GGCC 2 cut(s) 5, 160
BtgI CCRYGG 2 cut(s) 21, 151
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 195, 270
CaiI CAGNNNCTG 1 cut(s) 17
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 2 cut(s) 233, 268
Cfr13I GGNCC 1 cut(s) 158
Cfr42I CCGCGG 1 cut(s) 24
Csp6I GTAC 3 cut(s) 46, 94, 147
CviAII CATG 1 cut(s) 284
CviQI GTAC 3 cut(s) 46, 94, 147
DdeI CTNAG 3 cut(s) 162, 188, 273
DinI GGCGCC 1 cut(s) 89
DpnI GATC 2 cut(s) 417, 458
DpnII GATC 2 cut(s) 415, 456
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Eco32I GATATC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 158
EcoRV GATATC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 89
EheI GGCGCC 1 cut(s) 89
FaeI CATG 1 cut(s) 287
FaiI YATR 2 cut(s) 285, 405
FalI AAGNNNNNCTT 2 cut(s) 181, 213
FatI CATG 1 cut(s) 283
FauI CCCGC 1 cut(s) 186
Fnu4HI GCNGC 7 cut(s) 6, 9, 18, 21, 86, 308, 311
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 7 cut(s) 6, 9, 18, 21, 86, 308, 311
FspBI CTAG 1 cut(s) 460
GlaI GCGC 1 cut(s) 89
GluI GCNGC 7 cut(s) 6, 9, 18, 21, 86, 308, 311
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 2 cut(s) 5, 160
HapII CCGG 3 cut(s) 37, 234, 269
HhaI GCGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 1 cut(s) 287
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HpaII CCGG 3 cut(s) 37, 234, 269
HphI GGTGA 1 cut(s) 123
Hpy166II GTNNAC 2 cut(s) 46, 247
Hpy188I TCNGA 2 cut(s) 64, 77
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 46, 247
HpyAV CCTTC 3 cut(s) 200, 431, 447
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 11, 247, 399
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 17, 108, 224, 253, 274, 304, 316
HpyF3I CTNAG 3 cut(s) 162, 188, 273
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 1 cut(s) 287
HspAI GCGC 1 cut(s) 88
KasI GGCGCC 1 cut(s) 87
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 2 cut(s) 415, 456
LmnI GCTCC 3 cut(s) 14, 82, 201
LpnPI CCDG 3 cut(s) 50, 247, 282
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 328
MaeI CTAG 1 cut(s) 460
MaeIII GTNAC 1 cut(s) 129
MalI GATC 2 cut(s) 417, 458
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 415, 456
MboII GAAGA 4 cut(s) 231, 252, 373, 416
MfeI CAATTG 1 cut(s) 326
MflI RGATCY 2 cut(s) 415, 456
MhlI GDGCHC 2 cut(s) 118, 249
MluCI AATT 2 cut(s) 326, 448
Mly113I GGCGCC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 268
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 3 cut(s) 8, 23, 307
MspI CCGG 3 cut(s) 37, 234, 269
MunI CAATTG 1 cut(s) 326
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 8 cut(s) 14, 17, 108, 224, 253, 274, 304, 316
NaeI GCCGGC 1 cut(s) 270
NarI GGCGCC 1 cut(s) 88
NdeII GATC 2 cut(s) 415, 456
NgoMIV GCCGGC 1 cut(s) 268
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 89, 417
NmuCI GTSAC 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 270
PkrI GCNGC 7 cut(s) 7, 10, 19, 22, 87, 309, 312
PluTI GGCGCC 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 89, 417
PspPI GGNCC 1 cut(s) 158
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 415, 456
RsaI GTAC 3 cut(s) 47, 95, 148
RsaNI GTAC 3 cut(s) 46, 94, 147
RseI CAYNNNNRTG 1 cut(s) 282
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 7 cut(s) 6, 9, 18, 21, 86, 308, 311
Sau3AI GATC 2 cut(s) 415, 456
Sau96I GGNCC 1 cut(s) 158
SduI GDGCHC 2 cut(s) 118, 249
SetI ASST 3 cut(s) 19, 136, 390
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 9
SfoI GGCGCC 1 cut(s) 89
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 282
Sse9I AATT 2 cut(s) 326, 448
SspDI GGCGCC 1 cut(s) 87
SspMI CTAG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 107
TasI AATT 2 cut(s) 326, 448
TauI GCSGC 5 cut(s) 8, 23, 88, 310, 313
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 2 cut(s) 8, 17
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 1 cut(s) 168
VneI GTGCAC 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
XspI CTAG 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.