pycom15g14230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012962
Physical Location & Seq
Forward (+)
9689557 .. 9689949
393 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14230.1

Sequence Viewer

Length: 393 bp
ATGGCCGCTGCAGGAGCTGCCGCGGACGGGCTGTTCCGGTGCGTGTACGAGGGTTGCATATCGGGGTCGGATATCGGAGTGGAACGGCGCCCGTACCACCGCAACTGCGGGTGTGCCCTACACAACAAGTCACGTAACAAGCAATGCACCCACGGAGGGCCTAAGAGTAAGAAAGTGTCGTATCCGCTGAGGCGGGCTTGGAGCGAAGGGAACTTGGCTTTGGTGGCTTCCGCCGGTTCTTCCGTGCACTCCTCGCCGTCTTCCTCGCCGGCTCAGCACAACCACGCGGTCGGGAGGGCACATCATCGGCGGCAGTCGCATCAACATTTGGATACCTTGTGTGATGAGGATGAGGAGGATGAGGAGGATGAGATTGTTTTCTTCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.18

Weight (kDa)

7.14

Isoelectric Point (pI)

68.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012962)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46490 AT5G35110
fragaria_vesca FvH4_1g01610
malus_domestica MD02G1012800.v1.1 MD15G1158600.v1.1
prunus_persica Prupe.7G253800_v2.0.a1
pyrus_communis pycom15g14230
rosa_chinensis RchiOBHm_Chr2g0086561
rosa_laevigata RLG00000015767
rosa_multiflora Rmu_co8199806.1_g000001 Rmu_ssc0000449.1_g000004
rosa_roxburghii Rroxscaffold_2G00154440
rosa_rugosa Rorug01G0467900
rosa_samantha Rh2AG018900 Rh2BG018500 Rh2CG019400 Rh2DG020200
rosa_wichuraiana Rw2G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AccII CGCG 2 cut(s) 23, 287
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 88
AfaI GTAC 2 cut(s) 47, 95
AfiI CCNNNNNNNGG 2 cut(s) 27, 156
AgsI TTSAA 1 cut(s) 385
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw21I GWGCWC 1 cut(s) 249
Alw44I GTGCAC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 2 cut(s) 3, 158
ApaLI GTGCAC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 8, 17
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 158
BaeGI GKGCMC 3 cut(s) 118, 249, 301
BanI GGYRCC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 4
BceAI ACGGC 2 cut(s) 101, 241
BciVI GTATCC 2 cut(s) 192, 325
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 91
BfuI GTATCC 2 cut(s) 192, 325
BisI GCNGC 5 cut(s) 6, 9, 18, 21, 311
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 5 cut(s) 7, 10, 19, 22, 312
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 89
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 21, 151
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 156
Bse118I RCCGGY 2 cut(s) 233, 268
Bse3DI GCAATG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 21, 151
BseGI GGATG 3 cut(s) 355, 364, 373
BseLI CCNNNNNNNGG 2 cut(s) 27, 156
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 179, 287
BseRI GAGGAG 3 cut(s) 241, 368, 377
BseSI GKGCMC 3 cut(s) 118, 249, 301
BseXI GCAGC 1 cut(s) 4
Bsh1236I CGCG 2 cut(s) 23, 287
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 2 cut(s) 5, 160
BshNI GGYRCC 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 3 cut(s) 37, 234, 269
BslI CCNNNNNNNGG 2 cut(s) 27, 156
BsnI GGCC 2 cut(s) 5, 160
Bsp1286I GDGCHC 3 cut(s) 118, 249, 301
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 2 cut(s) 5, 160
BspCNI CTCAG 2 cut(s) 180, 286
BspFNI CGCG 2 cut(s) 23, 287
BspLI GGNNCC 1 cut(s) 89
BspMAI CTGCAG 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 149
BsrFI RCCGGY 2 cut(s) 233, 268
BssAI RCCGGY 2 cut(s) 233, 268
BssECI CCNNGG 2 cut(s) 21, 151
BssNI GRCGYC 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 195, 270
BstDEI CTNAG 3 cut(s) 162, 188, 273
BstDSI CCRYGG 2 cut(s) 21, 151
BstF5I GGATG 3 cut(s) 355, 364, 373
BstFNI CGCG 2 cut(s) 23, 287
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 6 cut(s) 14, 17, 224, 253, 274, 316
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 3 cut(s) 118, 249, 301
BstUI CGCG 2 cut(s) 23, 287
BstV1I GCAGC 1 cut(s) 4
BstV2I GAAGAC 1 cut(s) 252
BsuI GTATCC 2 cut(s) 192, 325
BsuRI GGCC 2 cut(s) 5, 160
BtgI CCRYGG 2 cut(s) 21, 151
BtsCI GGATG 3 cut(s) 355, 364, 373
Cac8I GCNNGC 2 cut(s) 195, 270
CaiI CAGNNNCTG 1 cut(s) 17
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 2 cut(s) 233, 268
Cfr13I GGNCC 1 cut(s) 158
Cfr42I CCGCGG 1 cut(s) 24
Csp6I GTAC 2 cut(s) 46, 94
CviJI RGCY 8 cut(s) 5, 17, 31, 160, 197, 218, 227, 272
CviKI_1 RGCY 8 cut(s) 5, 17, 31, 160, 197, 218, 227, 272
CviQI GTAC 2 cut(s) 46, 94
DdeI CTNAG 3 cut(s) 162, 188, 273
DinI GGCGCC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 220
Eco32I GATATC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 158
EcoRV GATATC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 89
EheI GGCGCC 1 cut(s) 89
FaiI YATR 1 cut(s) 59
FauI CCCGC 2 cut(s) 101, 186
Fnu4HI GCNGC 5 cut(s) 6, 9, 18, 21, 311
FokI GGATG 3 cut(s) 362, 371, 380
Fsp4HI GCNGC 5 cut(s) 6, 9, 18, 21, 311
GlaI GCGC 1 cut(s) 89
GluI GCNGC 5 cut(s) 6, 9, 18, 21, 311
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 2 cut(s) 5, 160
HapII CCGG 3 cut(s) 37, 234, 269
HhaI GCGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 88
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HpaII CCGG 3 cut(s) 37, 234, 269
Hpy166II GTNNAC 2 cut(s) 46, 247
Hpy188I TCNGA 2 cut(s) 70, 77
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 46, 247
HpyAV CCTTC 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 4 cut(s) 11, 57, 147, 247
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 17, 224, 253, 274, 316
HpyF3I CTNAG 3 cut(s) 162, 188, 273
HpySE526I ACGT 1 cut(s) 133
Hsp92I GRCGYC 1 cut(s) 88
HspAI GCGC 1 cut(s) 88
KasI GGCGCC 1 cut(s) 87
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
KspI CCGCGG 1 cut(s) 24
LmnI GCTCC 2 cut(s) 14, 201
LpnPI CCDG 3 cut(s) 50, 247, 282
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 328
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 129, 134
MboII GAAGA 3 cut(s) 231, 252, 373
MhlI GDGCHC 3 cut(s) 118, 249, 301
Mly113I GGCGCC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 268
MspA1I CMGCKG 3 cut(s) 8, 23, 187
MspI CCGG 3 cut(s) 37, 234, 269
MvnI CGCG 2 cut(s) 23, 287
MwoI GCNNNNNNNGC 6 cut(s) 14, 17, 224, 253, 274, 316
NaeI GCCGGC 1 cut(s) 270
NarI GGCGCC 1 cut(s) 88
NgoMIV GCCGGC 1 cut(s) 268
NlaIV GGNNCC 1 cut(s) 89
NmuCI GTSAC 1 cut(s) 129
PdiI GCCGGC 1 cut(s) 270
PkrI GCNGC 5 cut(s) 7, 10, 19, 22, 312
PluTI GGCGCC 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 158
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 47, 95
RsaNI GTAC 2 cut(s) 46, 94
SacII CCGCGG 1 cut(s) 24
SatI GCNGC 5 cut(s) 6, 9, 18, 21, 311
Sau96I GGNCC 1 cut(s) 158
SduI GDGCHC 3 cut(s) 118, 249, 301
SetI ASST 4 cut(s) 19, 136, 338, 390
SfaNI GCATC 1 cut(s) 328
SfcI CTRYAG 1 cut(s) 9
SfoI GGCGCC 1 cut(s) 89
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SspDI GGCGCC 1 cut(s) 87
TaiI ACGT 1 cut(s) 136
TauI GCSGC 3 cut(s) 8, 23, 313
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 2 cut(s) 8, 17
Tsp45I GTSAC 1 cut(s) 129
TspGWI ACGGA 2 cut(s) 168, 232
VneI GTGCAC 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.