Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009945

Physical Location & Seq

Genomic Coordinates Reverse (-)

1440485 .. 1444564

Gene Structure 4080 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1020200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGTCGTCGATGACCAAGATCACTCATCCCCGCCAACAGATCAACGGCCAGATTTTGCCCCATCCCTGTCCCCACCTAATCGAGTTCCTCTCCAACAACGGCTCCAAGCCCTTCCGCGCCTTACAGGACTGTCTCCGGGTCAAACCGCCCGGTGGCCGCGCCTCCATCCGCCGCGATCCCAAGGAAGTCCCGCGCTGTGGCGCGTGCGGGGAGTCTGCGCGTCCCAGGCTCTATGCGTGCATCACGTGCGCTGCCGTCCACTGTCACGTGCCATCCGGACCATCCCACGCGGCGGCTCACGCCCTCTCGATGCGTCCCGGTCACGAGATCGCCGTCGACGTGGACCGAGCCGAGCTCTTCTGCTGCGCGTGTAGGGACCAAGTCTACGATCGCGACTTAGACGCCGCAGTGGTCTTGGCTCAGACCGCCGCTTCGACGCTAGCATCCTCCGCCGCGTCAACGGTCCAGCAGTACGCGCCGGAGAATCTCCGGAAGCGACGCCGTATCGATTACAAGCCGTGGATGCCTGATCTGAGAGAGCAATACTTGGTGGGAAGAAATTCCAGTCCTCTAAACGGCGACGTTTCAGATTTACCTTGGGGATTGCGCGGGCTCAACAATTTGGGAAACACGTGCTTTATGAACTCGGTTCTGCAGGCATTGCTCCACACGCCGCCGCTGCGGAACTACTTCCTCAGCGATCGGCACAACCGCTACTTCTGCCAGAAGAAGAGCAATGCTGAGAACGCGGGGAAGAAAGCCACCGCCAATAATATTGAAAGTGGCGGGAACAAGGGTGCTGCGCGTGTGTGCTTGGCCTGCGACATGGACGCCACGTTTTCGGCGGTTTTTTCAGGGGATCGGACGCCTTATAGCCCCGCAAAGTTCTTGTACAGTTGGTGGCAGTACGCTGCGAATTTGGCGAGTTATGAGCAGCAGGACGCGCATGAGTTCTTCATTTCTATGCTTGATGGGATTCATGAGAAGGTGGATAAGGATCAGCGGAGACCCGAGAGCCAAGGCAATGGAGATTGTTGTGTTGCTCATAGAGTTTTTTCTGGTATCTTGCGATCTGATGTCATGTGTATGGCCTGTGGTTTTACGTCTACAACATATGACCCATGTGTGGACATCTCACTGGATTTGGAACCTAACCAAGGAGGTTCTGCAAAGACTGGGTCCACGAAGTCAAGTCATTCTTGCAATGGCGAGGCGGATTGCATGAATTCCAGCCAAAACTGTGGAGTATCTACCCTCATGGGATGCTTGGACCGTTTTACGAGACCTGAGAGATTGGGCTCTGACCAGAAATTTTTTTGCCAGCAGTGTCAGGTGAGGCAGGAGTCTCTCAAGCAAATGTCCATAAGAAAGCTTCCTTTGGTTTCGTGCTTCCATATCAAGCGATTTGAACATTCTTCGGTGAGGAAAATGTCAAGGAAGGTTGACCGTTATTTGCAGTTCCCGTTTTCACTCGACATGGCTCCTTATCTTTCATCTTCCATCTTGAGGAGCCGATTTGGAAATAGGATTTTGCCTTTTGATGGGGACGAGCCTGATGCATCAAATGACTTGTGTTCAGATTTTGAGTTGTTTGCTGTCGTCACTCACACAGGCAAGCTCGATGCAGGCCATTACGTGACTTACTTGCGATTGAGTAATCAATGGTACAAGTGCGACGATGCTTGGATCACACTAGTTAATGAGAACATTGTGAGGGCAGCACAGGGCTACATGATGTTTTATGTACAGAAAATGCTGTTTTACAAGGCAAGTGAGAAACCGGGTCCTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

598

Amino Acids

66.65

Weight (kDa)

8.56

Isoelectric Point (pI)

52.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	66 - 132	2.7e-10	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	203 - 581	6.4e-58	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	204 - 560	7.6e-16	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009945)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02230
malus_domestica	MD02G1020200.v1.1 MD15G1163000.v1.1
prunus_persica	Prupe.7G251600_v2.0.a1
pyrus_communis	pycom15g14660
rosa_chinensis	RchiOBHm_Chr2g0087351 RchiOBHm_Chr2g0087361
rosa_laevigata	RLG00000015859
rosa_multiflora	Rmu_co8225392.1_g000001 Rmu_co8488985.1_g000001 Rmu_sc0003367.1_g000026 Rmu_sc0006064.1_g000003 Rmu_sc0033647.1_g000002
rosa_roxburghii	Rroxscaffold_2G00153670
rosa_rugosa	Rorug01G0474400
rosa_samantha	Rh2AG028000 Rh2AG028100 Rh2BG027500 Rh2CG028300 Rh2DG028100
rosa_wichuraiana	Rw2G002220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	336, 384, 1106
AccIII	TCCGGA	2 cut(s)	275, 489
AclWI	GGATC	4 cut(s)	170, 867, 1005, 1694
AcoI	YGGCCR	2 cut(s)	46, 154
AcsI	RAATTY	4 cut(s)	559, 916, 1225, 1310
AcvI	CACGTG	3 cut(s)	246, 268, 633
AcyI	GRCGYC	4 cut(s)	402, 499, 831, 866
AfaI	GTAC	5 cut(s)	473, 893, 908, 1667, 1746
AfiI	CCNNNNNNNGG	8 cut(s)	152, 197, 292, 575, 1126, 1157, 1506, 1541
AflIII	ACRYGT	1 cut(s)	630
AgsI	TTSAA	2 cut(s)	779, 1409
AhlI	ACTAGT	1 cut(s)	1693
AjiI	CACGTC	1 cut(s)	340
AjnI	CCWGG	1 cut(s)	224
AluBI	AGCT	3 cut(s)	355, 1372, 1618
AluI	AGCT	3 cut(s)	355, 1372, 1618
Alw21I	GWGCWC	1 cut(s)	357
Alw26I	GTCTC	4 cut(s)	137, 1000, 1276, 1350
AlwI	GGATC	4 cut(s)	170, 867, 1005, 1694
Ama87I	CYCGRG	1 cut(s)	1010
Aor13HI	TCCGGA	2 cut(s)	275, 489
AoxI	GGCC	5 cut(s)	46, 154, 816, 1089, 1627
ApeKI	GCWGC	7 cut(s)	251, 363, 679, 800, 911, 934, 1718
ApoI	RAATTY	4 cut(s)	559, 916, 1225, 1310
ArsI	GACNNNNNNTTYG	4 cut(s)	415, 447, 1163, 1195
Asp700I	GAANNNNTTC	2 cut(s)	559, 689
AspS9I	GGNCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
AsuC2I	CCSGG	4 cut(s)	137, 150, 318, 1782
AsuHPI	GGTGA	2 cut(s)	1345, 1432
AsuNHI	GCTAGC	1 cut(s)	439
AvaI	CYCGRG	1 cut(s)	1010
AvaII	GGWCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
BanII	GRGCYC	3 cut(s)	357, 615, 1301
BauI	CACGAG	1 cut(s)	323
BbrPI	CACGTG	3 cut(s)	246, 268, 633
Bbv12I	GWGCWC	1 cut(s)	357
BbvCI	CCTCAGC	1 cut(s)	695
BbvI	GCAGC	7 cut(s)	238, 350, 666, 787, 898, 946, 1730
BccI	CCATC	7 cut(s)	69, 173, 280, 289, 965, 1508, 1535
BceAI	ACGGC	7 cut(s)	61, 115, 239, 317, 486, 502, 592
BcgI	CGANNNNNNTGC	2 cut(s)	1538, 1572
BciT130I	CCWGG	1 cut(s)	226
BcnI	CCSGG	4 cut(s)	137, 150, 318, 1782
BcoDI	GTCTC	4 cut(s)	137, 1000, 1276, 1350
BcuI	ACTAGT	1 cut(s)	1693
BfaI	CTAG	2 cut(s)	440, 1694
BfmI	CTRYAG	1 cut(s)	653
Bme1390I	CCNGG	5 cut(s)	137, 150, 226, 318, 1782
Bme18I	GGWCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
BmeT110I	CYCGRG	1 cut(s)	1010
BmgBI	CACGTC	1 cut(s)	340
BmgT120I	GGNCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
BmiI	GGNNCC	7 cut(s)	103, 377, 1149, 1180, 1482, 1511, 1785
BmrFI	CCNGG	5 cut(s)	137, 150, 226, 318, 1782
BmrI	ACTGGG	1 cut(s)	1185
BmsI	GCATC	9 cut(s)	249, 300, 452, 513, 1253, 1546, 1568, 1612, 1669
BmtI	GCTAGC	1 cut(s)	443
BmuI	ACTGGG	1 cut(s)	1185
BplI	GAGNNNNNCTC	4 cut(s)	74, 106, 339, 371
Bpu10I	CCTNAGC	1 cut(s)	695
BpuEI	CTTGAG	2 cut(s)	1334, 1525
BpuMI	CCSGG	4 cut(s)	137, 150, 318, 1782
Bsa29I	ATCGAT	1 cut(s)	507
BsaAI	YACGTR	4 cut(s)	246, 268, 633, 1636
BsaBI	GATNNNNATC	1 cut(s)	996
BsaHI	GRCGYC	4 cut(s)	402, 499, 831, 866
BsaI	GGTCTC	2 cut(s)	1000, 1276
BsaJI	CCNNGG	6 cut(s)	180, 224, 518, 596, 1018, 1156
BsaWI	WCCGGW	2 cut(s)	275, 489
BsaXI	ACNNNNNCTCC	2 cut(s)	86, 116
Bsc4I	CCNNNNNNNGG	8 cut(s)	152, 197, 292, 575, 1126, 1157, 1506, 1541
Bse1I	ACTGG	3 cut(s)	564, 1143, 1180
Bse3DI	GCAATG	4 cut(s)	659, 742, 1030, 1210
Bse8I	GATNNNNATC	1 cut(s)	996
BseAI	TCCGGA	2 cut(s)	275, 489
BseBI	CCWGG	1 cut(s)	226
BseCI	ATCGAT	1 cut(s)	507
BseDI	CCNNGG	6 cut(s)	180, 224, 518, 596, 1018, 1156
BseGI	GGATG	8 cut(s)	25, 61, 165, 272, 281, 443, 528, 1268
BseJI	GATNNNNATC	1 cut(s)	996
BseLI	CCNNNNNNNGG	8 cut(s)	152, 197, 292, 575, 1126, 1157, 1506, 1541
BseMI	GCAATG	4 cut(s)	659, 742, 1030, 1210
BseMII	CTCAG	5 cut(s)	434, 524, 709, 732, 1278
BseNI	ACTGG	3 cut(s)	564, 1143, 1180
BseRI	GAGGAG	1 cut(s)	1522
BseXI	GCAGC	7 cut(s)	238, 350, 666, 787, 898, 946, 1730
Bsh1285I	CGRYCG	2 cut(s)	391, 703
BshFI	GGCC	5 cut(s)	48, 156, 818, 1091, 1629
BshVI	ATCGAT	1 cut(s)	507
BsiEI	CGRYCG	2 cut(s)	391, 703
BsiHKAI	GWGCWC	1 cut(s)	357
BsiHKCI	CYCGRG	1 cut(s)	1010
BsiSI	CCGG	7 cut(s)	136, 150, 276, 318, 479, 490, 1781
BslFI	GGGAC	6 cut(s)	54, 173, 207, 300, 389, 1559
BslI	CCNNNNNNNGG	8 cut(s)	152, 197, 292, 575, 1126, 1157, 1506, 1541
BsmAI	GTCTC	4 cut(s)	137, 1000, 1276, 1350
BsmFI	GGGAC	6 cut(s)	54, 173, 207, 300, 389, 1559
BsnI	GGCC	5 cut(s)	48, 156, 818, 1091, 1629
Bso31I	GGTCTC	2 cut(s)	1000, 1276
BsoBI	CYCGRG	1 cut(s)	1010
Bsp1286I	GDGCHC	3 cut(s)	357, 615, 1301
Bsp13I	TCCGGA	2 cut(s)	275, 489
Bsp1407I	TGTACA	2 cut(s)	891, 1744
Bsp68I	TCGCGA	1 cut(s)	393
BspANI	GGCC	5 cut(s)	48, 156, 818, 1091, 1629
BspCNI	CTCAG	5 cut(s)	433, 525, 708, 733, 1279
BspDI	ATCGAT	1 cut(s)	507
BspEI	TCCGGA	2 cut(s)	275, 489
BspHI	TCATGA	1 cut(s)	979
BspLI	GGNNCC	7 cut(s)	103, 377, 1149, 1180, 1482, 1511, 1785
BspMAI	CTGCAG	1 cut(s)	657
BspOI	GCTAGC	1 cut(s)	443
BspPI	GGATC	4 cut(s)	170, 867, 1005, 1694
BspQI	GCTCTTC	2 cut(s)	362, 725
BspTNI	GGTCTC	2 cut(s)	1000, 1276
BsrDI	GCAATG	4 cut(s)	659, 742, 1030, 1210
BsrGI	TGTACA	2 cut(s)	891, 1744
BsrI	ACTGG	3 cut(s)	564, 1143, 1180
BssECI	CCNNGG	6 cut(s)	180, 224, 518, 596, 1018, 1156
BssNI	GRCGYC	4 cut(s)	402, 499, 831, 866
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
Bst2BI	CACGAG	1 cut(s)	323
Bst2UI	CCWGG	1 cut(s)	226
Bst4CI	ACNGT	7 cut(s)	131, 263, 463, 896, 1241, 1274, 1448
Bst6I	CTCTTC	2 cut(s)	362, 725
BstACI	GRCGYC	4 cut(s)	402, 499, 831, 866
BstAPI	GCANNNNNTGC	2 cut(s)	246, 661
BstAUI	TGTACA	2 cut(s)	891, 1744
BstBAI	YACGTR	4 cut(s)	246, 268, 633, 1636
BstC8I	GCNNGC	9 cut(s)	205, 238, 441, 611, 657, 820, 1322, 1616, 1627
BstDEI	CTNAG	6 cut(s)	397, 420, 533, 695, 741, 1287
BstDSI	CCRYGG	1 cut(s)	518
BstENI	CCTNNNNNAGG	1 cut(s)	1155
BstF5I	GGATG	8 cut(s)	25, 61, 165, 272, 281, 443, 528, 1268
BstMAI	GTCTC	4 cut(s)	137, 1000, 1276, 1350
BstMCI	CGRYCG	2 cut(s)	391, 703
BstNI	CCWGG	1 cut(s)	226
BstSCI	CCNGG	5 cut(s)	135, 148, 224, 316, 1780
BstSFI	CTRYAG	1 cut(s)	653
BstV1I	GCAGC	7 cut(s)	238, 350, 666, 787, 898, 946, 1730
BstXI	CCANNNNNNTGG	2 cut(s)	1025, 1241
Bsu15I	ATCGAT	1 cut(s)	507
BsuRI	GGCC	5 cut(s)	48, 156, 818, 1091, 1629
BsuTUI	ATCGAT	1 cut(s)	507
BtgI	CCRYGG	1 cut(s)	518
BtrI	CACGTC	1 cut(s)	340
BtsCI	GGATG	8 cut(s)	25, 61, 165, 272, 281, 443, 528, 1268
BtsI	GCAGTG	2 cut(s)	414, 1331
BtsIMutI	CAGTG	4 cut(s)	259, 414, 1136, 1331
BtuMI	TCGCGA	1 cut(s)	393
Cac8I	GCNNGC	9 cut(s)	205, 238, 441, 611, 657, 820, 1322, 1616, 1627
CciI	TCATGA	1 cut(s)	979
Cfr13I	GGNCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
ClaI	ATCGAT	1 cut(s)	507
CseI	GACGC	9 cut(s)	209, 302, 410, 444, 445, 507, 839, 874, 950
Csp6I	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
CviAII	CATG	9 cut(s)	826, 947, 980, 1081, 1122, 1222, 1258, 1477, 1732
CviQI	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
DdeI	CTNAG	6 cut(s)	397, 420, 533, 695, 741, 1287
EaeI	YGGCCR	2 cut(s)	46, 154
Eam1104I	CTCTTC	2 cut(s)	362, 725
EarI	CTCTTC	2 cut(s)	362, 725
EciI	GGCGGA	3 cut(s)	158, 439, 1229
Ecl136II	GAGCTC	1 cut(s)	355
Eco130I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
Eco24I	GRGCYC	3 cut(s)	357, 615, 1301
Eco31I	GGTCTC	2 cut(s)	1000, 1276
Eco47I	GGWCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
Eco53kI	GAGCTC	1 cut(s)	355
Eco72I	CACGTG	3 cut(s)	246, 268, 633
Eco88I	CYCGRG	1 cut(s)	1010
EcoICRI	GAGCTC	1 cut(s)	355
EcoNI	CCTNNNNNAGG	1 cut(s)	1155
EcoO109I	RGGNCCY	1 cut(s)	1784
EcoRI	GAATTC	1 cut(s)	1225
EcoRII	CCWGG	1 cut(s)	224
EcoT14I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
EcoT22I	ATGCAT	1 cut(s)	1561
EcoT38I	GRGCYC	3 cut(s)	357, 615, 1301
ErhI	CCWWGG	4 cut(s)	180, 596, 1018, 1156
FaeI	CATG	9 cut(s)	829, 950, 983, 1084, 1125, 1225, 1261, 1480, 1735
FalI	AAGNNNNNCTT	2 cut(s)	1183, 1215
FaqI	GGGAC	6 cut(s)	54, 173, 207, 300, 389, 1559
FatI	CATG	9 cut(s)	825, 946, 979, 1080, 1121, 1221, 1257, 1476, 1731
FauI	CCCGC	7 cut(s)	38, 198, 200, 602, 742, 779, 886
FauNDI	CATATG	1 cut(s)	1114
FblI	GTMKAC	3 cut(s)	336, 384, 1106
FokI	GGATG	8 cut(s)	12, 48, 152, 259, 268, 430, 535, 1275
FriOI	GRGCYC	3 cut(s)	357, 615, 1301
FspBI	CTAG	2 cut(s)	440, 1694
HaeIII	GGCC	5 cut(s)	48, 156, 818, 1091, 1629
HapII	CCGG	7 cut(s)	136, 150, 276, 318, 479, 490, 1781
HgaI	GACGC	9 cut(s)	209, 302, 410, 444, 445, 507, 839, 874, 950
Hin1I	GRCGYC	4 cut(s)	402, 499, 831, 866
Hin1II	CATG	9 cut(s)	829, 950, 983, 1084, 1125, 1225, 1261, 1480, 1735
HincII	GTYRAC	3 cut(s)	337, 459, 1444
HindII	GTYRAC	3 cut(s)	337, 459, 1444
HindIII	AAGCTT	1 cut(s)	1370
HinfI	GANTC	4 cut(s)	212, 484, 976, 1343
HpaII	CCGG	7 cut(s)	136, 150, 276, 318, 479, 490, 1781
HphI	GGTGA	2 cut(s)	1345, 1432
Hpy166II	GTNNAC	9 cut(s)	259, 337, 343, 385, 459, 1107, 1129, 1182, 1444
Hpy188I	TCNGA	7 cut(s)	423, 534, 589, 864, 1075, 1303, 1579
Hpy188III	TCNNGA	7 cut(s)	276, 307, 323, 392, 490, 980, 1504
Hpy8I	GTNNAC	9 cut(s)	259, 337, 343, 385, 459, 1107, 1129, 1182, 1444
Hpy99I	CGWCG	7 cut(s)	10, 338, 341, 439, 501, 584, 1679
HpyAV	CCTTC	3 cut(s)	121, 979, 1432
HpyCH4III	ACNGT	7 cut(s)	131, 263, 463, 896, 1241, 1274, 1448
HpyCH4IV	ACGT	8 cut(s)	245, 267, 339, 582, 632, 836, 1103, 1635
HpyCH4V	TGCA	8 cut(s)	240, 655, 1169, 1203, 1221, 1456, 1559, 1625
HpyF3I	CTNAG	6 cut(s)	397, 420, 533, 695, 741, 1287
HpySE526I	ACGT	8 cut(s)	245, 267, 339, 582, 632, 836, 1103, 1635
Hsp92I	GRCGYC	4 cut(s)	402, 499, 831, 866
Hsp92II	CATG	9 cut(s)	829, 950, 983, 1084, 1125, 1225, 1261, 1480, 1735
Kpn2I	TCCGGA	2 cut(s)	275, 489
LguI	GCTCTTC	2 cut(s)	362, 725
LmnI	GCTCC	4 cut(s)	107, 669, 1486, 1509
Lsp1109I	GCAGC	7 cut(s)	238, 350, 666, 787, 898, 946, 1730
LweI	GCATC	9 cut(s)	249, 300, 452, 513, 1253, 1546, 1568, 1612, 1669
MaeI	CTAG	2 cut(s)	440, 1694
MaeII	ACGT	8 cut(s)	245, 267, 339, 582, 632, 836, 1103, 1635
MaeIII	GTNAC	4 cut(s)	263, 320, 1600, 1636
MboII	GAAGA	8 cut(s)	349, 567, 739, 742, 766, 946, 1407, 1488
MhlI	GDGCHC	3 cut(s)	357, 615, 1301
MluCI	AATT	5 cut(s)	559, 619, 916, 1225, 1310
MlyI	GAGTC	2 cut(s)	221, 1352
MmeI	TCCRAC	1 cut(s)	117
Mph1103I	ATGCAT	1 cut(s)	1561
MroI	TCCGGA	2 cut(s)	275, 489
MroXI	GAANNNNTTC	2 cut(s)	559, 689
MseI	TTAA	1 cut(s)	1698
MslI	CAYNNNNRTG	2 cut(s)	962, 1085
MspA1I	CMGCKG	2 cut(s)	679, 1003
MspI	CCGG	7 cut(s)	136, 150, 276, 318, 479, 490, 1781
MspR9I	CCNGG	5 cut(s)	137, 150, 226, 318, 1782
MvaI	CCWGG	1 cut(s)	226
NciI	CCSGG	4 cut(s)	137, 150, 318, 1782
NdeI	CATATG	1 cut(s)	1114
NheI	GCTAGC	1 cut(s)	439
NlaIII	CATG	9 cut(s)	829, 950, 983, 1084, 1125, 1225, 1261, 1480, 1735
NlaIV	GGNNCC	7 cut(s)	103, 377, 1149, 1180, 1482, 1511, 1785
NmeAIII	GCCGAG	1 cut(s)	376
NmuCI	GTSAC	4 cut(s)	263, 320, 1600, 1636
NruI	TCGCGA	1 cut(s)	393
NsiI	ATGCAT	1 cut(s)	1561
PagI	TCATGA	1 cut(s)	979
PciSI	GCTCTTC	2 cut(s)	362, 725
PcsI	WCGNNNNNNNCGW	2 cut(s)	330, 336
PdmI	GAANNNNTTC	2 cut(s)	559, 689
PfeI	GAWTC	2 cut(s)	484, 976
PflFI	GACNNNGTC	2 cut(s)	380, 1177
Ple19I	CGATCG	2 cut(s)	391, 703
PleI	GAGTC	2 cut(s)	220, 1351
PmaCI	CACGTG	3 cut(s)	246, 268, 633
PmlI	CACGTG	3 cut(s)	246, 268, 633
PpsI	GAGTC	2 cut(s)	220, 1351
Ppu21I	YACGTR	4 cut(s)	246, 268, 633, 1636
PpuMI	RGGWCCY	1 cut(s)	1784
Psp124BI	GAGCTC	1 cut(s)	357
Psp5II	RGGWCCY	1 cut(s)	1784
Psp6I	CCWGG	1 cut(s)	224
PspCI	CACGTG	3 cut(s)	246, 268, 633
PspGI	CCWGG	1 cut(s)	224
PspN4I	GGNNCC	7 cut(s)	103, 377, 1149, 1180, 1482, 1511, 1785
PspPI	GGNCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
PspPPI	RGGWCCY	1 cut(s)	1784
PstI	CTGCAG	1 cut(s)	657
PsyI	GACNNNGTC	2 cut(s)	380, 1177
PvuI	CGATCG	2 cut(s)	391, 703
RruI	TCGCGA	1 cut(s)	393
RsaI	GTAC	5 cut(s)	473, 893, 908, 1667, 1746
RsaNI	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
RseI	CAYNNNNRTG	2 cut(s)	962, 1085
SacI	GAGCTC	1 cut(s)	357
SalI	GTCGAC	1 cut(s)	335
SapI	GCTCTTC	2 cut(s)	362, 725
SaqAI	TTAA	1 cut(s)	1698
Sau96I	GGNCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
SchI	GAGTC	2 cut(s)	221, 1352
ScrFI	CCNGG	5 cut(s)	137, 150, 226, 318, 1782
SduI	GDGCHC	3 cut(s)	357, 615, 1301
SfaNI	GCATC	9 cut(s)	249, 300, 452, 513, 1253, 1546, 1568, 1612, 1669
SfcI	CTRYAG	1 cut(s)	653
SgrDI	CGTCGACG	1 cut(s)	335
SinI	GGWCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
SmiMI	CAYNNNNRTG	2 cut(s)	962, 1085
SmlI	CTYRAG	2 cut(s)	1349, 1504
SmoI	CTYRAG	2 cut(s)	1349, 1504
SpeI	ACTAGT	1 cut(s)	1693
Sse9I	AATT	5 cut(s)	559, 619, 916, 1225, 1310
SspI	AATATT	1 cut(s)	775
SspMI	CTAG	2 cut(s)	440, 1694
SstI	GAGCTC	1 cut(s)	357
StyD4I	CCNGG	5 cut(s)	135, 148, 224, 316, 1780
StyI	CCWWGG	4 cut(s)	180, 596, 1018, 1156
TaaI	ACNGT	7 cut(s)	131, 263, 463, 896, 1241, 1274, 1448
TaiI	ACGT	8 cut(s)	248, 270, 342, 585, 635, 839, 1106, 1638
TaqI	TCGA	8 cut(s)	8, 81, 308, 336, 434, 507, 1473, 1620
TaqII	GACCGA	1 cut(s)	360
TasI	AATT	5 cut(s)	559, 619, 916, 1225, 1310
TatI	WGTACW	2 cut(s)	891, 1744
TauI	GCSGC	9 cut(s)	159, 174, 293, 296, 407, 431, 455, 676, 679
TfiI	GAWTC	2 cut(s)	484, 976
Tru1I	TTAA	1 cut(s)	1698
Tru9I	TTAA	1 cut(s)	1698
TscAI	CASTG	4 cut(s)	266, 414, 1143, 1331
TseFI	GTSAC	4 cut(s)	263, 320, 1600, 1636
TseI	GCWGC	7 cut(s)	251, 363, 679, 800, 911, 934, 1718
Tsp45I	GTSAC	4 cut(s)	263, 320, 1600, 1636
TspDTI	ATGAA	5 cut(s)	656, 946, 968, 1238, 1482
TspRI	CASTG	4 cut(s)	266, 414, 1143, 1331
Tth111I	GACNNNGTC	2 cut(s)	380, 1177
VpaK11BI	GGWCC	7 cut(s)	278, 343, 376, 463, 1179, 1270, 1784
XagI	CCTNNNNNAGG	1 cut(s)	1155
XapI	RAATTY	4 cut(s)	559, 916, 1225, 1310
XmiI	GTMKAC	3 cut(s)	336, 384, 1106
XmnI	GAANNNNTTC	2 cut(s)	559, 689
XspI	CTAG	2 cut(s)	440, 1694
Zsp2I	ATGCAT	1 cut(s)	1561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.