RchiOBHm_Chr2g0087361

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009945
Physical Location & Seq
Forward (+)
2130229 .. 2131514
1286 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46281

Sequence Viewer

Length: 705 bp
ATGTGTATGGCTTGTGGTTTTACGTCTACAACATATGATCCATGCGTAGACATCTCACTGGATTTGGAACCAGGTTCTGCAAAGACGATGTCCACAAAGTCGAATCATTCTTGCAATGGCAATGCAGATTGCATGAATTCTGGCCAAAACTGTGGCACATCTACCCTTGTGGGATGCCTGGACCGTTTTACAAGACCTGAGAGATTGGGCTCTGACCAGAAATTTTTTTGCCAGCAGTGTCAGGTGAGACAAGAGTCTCTCAAGCAAATGTCCATTAGGAAGCTTCCTTTAGTTTCTTGCTTCCATATCAAACGATTTGAGCATTCTTCAATACGAAAGATGTCAAGGAAGGTGGATCGTTATCTGCAGTTTCCATTTTCGTTGGACATGGCACCATATCTTTCGTCTTCCATCTTAAGGAATCGATTTGGGAATAGGATTCTTCCTTTTGATGGGGATGAGCCAGAAGCTCCAAATGAGTTATGCTCAGAATTTGAGTTGTTTGCTGTCGTAACTCACACAGGCAAGCTGGATGCTGGCCATTACGTGACTTACTTGCGATTAAGTAATCATTGGTACAAGTGTGATGATGCTTGGATCACACAAGTTAACGAGGACATTGTGAGGGCTGCACAAGGCTACATGATGTTTTATGTACAGAAAATGCTATTTTACAAAGCAAGTGAGAAACAGGGTTCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

234

Amino Acids

26.69

Weight (kDa)

7.95

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCH_1 PF13423 2 - 197 6.1e-07 Ubiquitin carboxyl-terminal hydrolase
UCH PF00443 75 - 218 1e-24 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 391
AccI GTMKAC 2 cut(s) 26, 48
AclWI GGATC 3 cut(s) 32, 363, 605
AcoI YGGCCR 2 cut(s) 142, 538
AcsI RAATTY 3 cut(s) 136, 221, 491
AfaI GTAC 2 cut(s) 578, 657
AfiI CCNNNNNNNGG 2 cut(s) 417, 452
AflII CTTAAG 1 cut(s) 415
AgsI TTSAA 1 cut(s) 330
AjnI CCWGG 2 cut(s) 70, 177
AluBI AGCT 3 cut(s) 283, 470, 529
AluI AGCT 3 cut(s) 283, 470, 529
Alw26I GTCTC 2 cut(s) 241, 261
AlwI GGATC 3 cut(s) 32, 363, 605
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 2 cut(s) 142, 538
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 3 cut(s) 136, 221, 491
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 181
BaeI ACNNNNGTAYC 2 cut(s) 568, 601
BalI TGGCCA 2 cut(s) 144, 540
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 616
BccI CCATC 2 cut(s) 419, 446
BciT130I CCWGG 2 cut(s) 72, 179
BcoDI GTCTC 2 cut(s) 241, 261
BfmI CTRYAG 1 cut(s) 365
BfrI CTTAAG 1 cut(s) 415
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
Bme1390I CCNGG 2 cut(s) 72, 179
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 69, 393
BmrFI CCNGG 2 cut(s) 72, 179
BmsI GCATC 3 cut(s) 164, 523, 580
BoxI GACNNNNGTC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BpuEI CTTGAG 1 cut(s) 245
Bsa29I ATCGAT 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 360
Bsc4I CCNNNNNNNGG 2 cut(s) 417, 452
Bse1I ACTGG 1 cut(s) 63
Bse3DI GCAATG 2 cut(s) 121, 127
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 2 cut(s) 72, 179
BseCI ATCGAT 1 cut(s) 424
BseGI GGATG 3 cut(s) 179, 463, 538
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 2 cut(s) 417, 452
BseMI GCAATG 2 cut(s) 121, 127
BseMII CTCAG 2 cut(s) 189, 501
BseNI ACTGG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 2 cut(s) 144, 540
BshNI GGYRCC 1 cut(s) 391
BshVI ATCGAT 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 417, 452
BsmAI GTCTC 2 cut(s) 241, 261
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 2 cut(s) 144, 540
Bsp1286I GDGCHC 1 cut(s) 212
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 3 cut(s) 37, 355, 597
BspANI GGCC 2 cut(s) 144, 540
BspCNI CTCAG 2 cut(s) 190, 500
BspDI ATCGAT 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 69, 393
BspMAI CTGCAG 1 cut(s) 369
BspPI GGATC 3 cut(s) 32, 363, 605
BspT107I GGYRCC 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 415
BsrDI GCAATG 2 cut(s) 121, 127
BsrGI TGTACA 1 cut(s) 655
BsrI ACTGG 1 cut(s) 63
BssMI GATC 3 cut(s) 37, 355, 597
Bst2UI CCWGG 2 cut(s) 72, 179
Bst4CI ACNGT 2 cut(s) 152, 185
BstAFI CTTAAG 1 cut(s) 415
BstAUI TGTACA 1 cut(s) 655
BstBAI YACGTR 1 cut(s) 547
BstC8I GCNNGC 3 cut(s) 233, 527, 538
BstDEI CTNAG 2 cut(s) 198, 487
BstF5I GGATG 3 cut(s) 179, 463, 538
BstKTI GATC 3 cut(s) 40, 358, 600
BstMAI GTCTC 2 cut(s) 241, 261
BstMBI GATC 3 cut(s) 37, 355, 597
BstNI CCWGG 2 cut(s) 72, 179
BstPAI GACNNNNGTC 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 70, 177
BstSFI CTRYAG 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 2 cut(s) 144, 540
BsuTUI ATCGAT 1 cut(s) 424
BtsCI GGATG 3 cut(s) 179, 463, 538
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 56, 242
Cac8I GCNNGC 3 cut(s) 233, 527, 538
CaiI CAGNNNCTG 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 181
ClaI ATCGAT 1 cut(s) 424
CsiI ACCWGGT 1 cut(s) 70
Csp6I GTAC 2 cut(s) 577, 656
CspCI CAANNNNNGTGG 2 cut(s) 333, 368
CviAII CATG 4 cut(s) 42, 133, 388, 643
CviQI GTAC 2 cut(s) 577, 656
DdeI CTNAG 2 cut(s) 198, 487
DpnI GATC 3 cut(s) 39, 357, 599
DpnII GATC 3 cut(s) 37, 355, 597
EaeI YGGCCR 2 cut(s) 142, 538
Eco24I GRGCYC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 70, 177
EcoT38I GRGCYC 1 cut(s) 212
FaeI CATG 4 cut(s) 45, 136, 391, 646
FatI CATG 4 cut(s) 41, 132, 387, 642
FauNDI CATATG 1 cut(s) 34
FblI GTMKAC 2 cut(s) 26, 48
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 3 cut(s) 186, 470, 545
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 1 cut(s) 630
GluI GCNGC 1 cut(s) 630
HaeIII GGCC 2 cut(s) 144, 540
Hin1II CATG 4 cut(s) 45, 136, 391, 646
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 103, 254, 421, 439
HpaI GTTAAC 1 cut(s) 610
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 4 cut(s) 27, 49, 93, 610
Hpy188I TCNGA 2 cut(s) 214, 490
Hpy8I GTNNAC 4 cut(s) 27, 49, 93, 610
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 2 cut(s) 152, 185
HpyCH4IV ACGT 2 cut(s) 23, 546
HpyCH4V TGCA 7 cut(s) 80, 114, 125, 132, 367, 632, 701
HpyF3I CTNAG 2 cut(s) 198, 487
HpySE526I ACGT 2 cut(s) 23, 546
Hsp92II CATG 4 cut(s) 45, 136, 391, 646
KspAI GTTAAC 1 cut(s) 610
Kzo9I GATC 3 cut(s) 37, 355, 597
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 3 cut(s) 164, 523, 580
MabI ACCWGGT 1 cut(s) 70
MaeII ACGT 2 cut(s) 23, 546
MaeIII GTNAC 2 cut(s) 511, 547
MalI GATC 3 cut(s) 39, 357, 599
MboI GATC 3 cut(s) 37, 355, 597
MboII GAAGA 3 cut(s) 318, 399, 434
MhlI GDGCHC 1 cut(s) 212
MlsI TGGCCA 2 cut(s) 144, 540
MluCI AATT 3 cut(s) 136, 221, 491
MluNI TGGCCA 2 cut(s) 144, 540
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 363
MnlI CCTC 2 cut(s) 607, 618
Mox20I TGGCCA 2 cut(s) 144, 540
MscI TGGCCA 2 cut(s) 144, 540
MseI TTAA 3 cut(s) 416, 563, 609
Msp20I TGGCCA 2 cut(s) 144, 540
MspCI CTTAAG 1 cut(s) 415
MspR9I CCNGG 2 cut(s) 72, 179
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 2 cut(s) 72, 179
NdeI CATATG 1 cut(s) 34
NdeII GATC 3 cut(s) 37, 355, 597
NlaIII CATG 4 cut(s) 45, 136, 391, 646
NlaIV GGNNCC 2 cut(s) 69, 393
NmuCI GTSAC 1 cut(s) 547
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 3 cut(s) 103, 421, 439
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 1 cut(s) 547
PshAI GACNNNNGTC 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 70, 177
PspGI CCWGG 2 cut(s) 70, 177
PspN4I GGNNCC 2 cut(s) 69, 393
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 77
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 2 cut(s) 578, 657
RsaNI GTAC 2 cut(s) 577, 656
SaqAI TTAA 3 cut(s) 416, 563, 609
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 3 cut(s) 37, 355, 597
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 72, 179
SduI GDGCHC 1 cut(s) 212
SetI ASST 9 cut(s) 26, 76, 199, 246, 285, 354, 472, 531, 549
SexAI ACCWGGT 1 cut(s) 70
SfaNI GCATC 3 cut(s) 164, 523, 580
SfcI CTRYAG 1 cut(s) 365
SinI GGWCC 1 cut(s) 181
SmlI CTYRAG 2 cut(s) 260, 415
SmoI CTYRAG 2 cut(s) 260, 415
Sse9I AATT 3 cut(s) 136, 221, 491
StyD4I CCNGG 2 cut(s) 70, 177
TaaI ACNGT 2 cut(s) 152, 185
TaiI ACGT 2 cut(s) 26, 549
TaqI TCGA 2 cut(s) 101, 424
TasI AATT 3 cut(s) 136, 221, 491
TatI WGTACW 1 cut(s) 655
TfiI GAWTC 3 cut(s) 103, 421, 439
Tru1I TTAA 3 cut(s) 416, 563, 609
Tru9I TTAA 3 cut(s) 416, 563, 609
TscAI CASTG 2 cut(s) 63, 242
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 1 cut(s) 149
TspRI CASTG 2 cut(s) 63, 242
Tth111I GACNNNGTC 1 cut(s) 88
Vha464I CTTAAG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 3 cut(s) 136, 221, 491
XmiI GTMKAC 2 cut(s) 26, 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.