Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009945

Physical Location & Seq

Genomic Coordinates Forward (+)

1839975 .. 1843191

Gene Structure 3217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G002220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGTCGTCGATGACCAAGATCAACGGCCACGCTCTACCCCAGCCCTGCTCCCACCTCGCCGCCTTCCGATCCAAAAACGGCTCCAAACCCTTCCGCGCCTTACAGGACTGCCTCCGCGTCAAGCCCCCCGGCGGCCGCGCCTCGATCCGCCGCGATCCCCACGAGCTCCCCCGATGCGCCGCCTGCGGCGAGTCAGCTCACTCGCGGCTCTACGCCTGCGTCACCTGCGCCGCGGTTCACTGCCACGCTCCCTCCGGCGGACTCTCCCACGCGGCGGCTCACGCCGAGTCGCTGCCTCCGGGGCACGAGATCGCCGTCGACGTCGACCGGGCCGAGCTCTTCTGCTGCGCGTGCAGGGATCAAGTCTACGACCGCGACTTCGACGCCGCCGTGGTCCTGGCTCAGACGGCCTCTCCCGCCACCGCCTCCTCCGCGATCCAGCAGTACGCGCCGGAGAATCTCCGGAAACGGCGTCGGATCGATTACAAGCCGTGGTCGCCGGATTTGAGTGAGCAGGTGATCGTCGGAAAAAACTCGAGCCTCCTCCACGGCCGGACTCCCTCCGCTTTGCCTTGGGGACTGCGCGGGCTCAACAATTTGGGGAACACGTGCTTCATGAACTCGGTTCTTCAGGCATTGCTCCACACGCCGCCGCTGCGGAACTACTTCCTCAGCGATCGGCACAACCGCTACTTCTGCCAGAAGAAGAGCAATGCCGATAAGAAAAGCGCGGGTAATAGTGGCGGCAACACGGCGGCCGGGTCTGTGTGCTTGGCCTGTGACATGGACGCCACGTTTTCGGCGGTTTTCTCAGGCGATCGGAAGCCTTATAGTCCAGCCAAGTTCTTGTACAGTTGGTGGCAATACGCTGCAAATTTGGCGAGCTATGAGCAGCAGGATGCGCATGAATTCTTCATTTCGATGCTGGATGGGATTCATGAGAAGGTGGAAAAGGAGGGGAGGAAACCCGGGAGCCAAGGCACTGGAGATTGTTGTGTGGCTCATAGAGTTTTTTCTGGCATCTTGCGATCTGATGTTATGTGTATGGCTTGTGGTTTTACGTCTACAACATATGATCCATGCGTAGACATCTCACTGGATTTGGAACCAGGTTCTGCAAAGACGATGTCCACAAAGTCGAATCATTCTTGCAATGGCAATGCAGATTGCATGAATTCTGGACAAAACTGTGGCACATCTACCCTTGTGGGATGCCTGGACCGTTTTACAAGACCTGAGAGATTGGGCTCTGACCAGAAATTTTTTTGCCAGCAGTGTCAGGTGAGACAAGAGTCTCTCAAGCAAATGTCCATTAGGAAGCTTCCTTTAGTTTCTTGCTTCCATATCAAACGATTTGAGCATTCTTCAATACGAAAGATGTCAAGGAAGGTGGATCGTTATCTGCAGTTCCCATTTTCGTTGGACATGGCACCATATCTTTCGTCTTCCATCTTAAGGAATCGATTTGGGAATAGGATTCTTCCTTTTGATGGGGATGAGCCAGAAGCTCCGAATGAGTTATGCTCAGAATTTGAGTTGTTTGCTGTCGTAACTCACACAGGCAAGCTGGATGCTGGCCATTACGTGACTTACTTGCGATTAAGTAATCATTGGTACAAGTGTGATGATGCTTGGATCACACAAGTTAACGAGGACATTGTGAGGGCTGCACAAGGCTACATGATGTTTTATGTACAGAAAATGCTATTTTACAAAGCAAGTGAGAAACAGGGTTCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

580

Amino Acids

64.37

Weight (kDa)

8.61

Isoelectric Point (pI)

49.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	59 - 126	8e-10	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	195 - 564	8.1e-59	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	196 - 543	2.1e-16	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009945)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02230
malus_domestica	MD02G1020200.v1.1 MD15G1163000.v1.1
prunus_persica	Prupe.7G251600_v2.0.a1
pyrus_communis	pycom15g14660
rosa_chinensis	RchiOBHm_Chr2g0087351 RchiOBHm_Chr2g0087361
rosa_laevigata	RLG00000015859
rosa_multiflora	Rmu_co8225392.1_g000001 Rmu_co8488985.1_g000001 Rmu_sc0003367.1_g000026 Rmu_sc0006064.1_g000003 Rmu_sc0033647.1_g000002
rosa_roxburghii	Rroxscaffold_2G00153670
rosa_rugosa	Rorug01G0474400
rosa_samantha	Rh2AG028000 Rh2AG028100 Rh2BG027500 Rh2CG028300 Rh2DG028100
rosa_wichuraiana	Rw2G002220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	233, 505
AatII	GACGTC	1 cut(s)	324
Acc16I	TGCGCA	1 cut(s)	903
Acc36I	ACCTGC	2 cut(s)	233, 505
AccB1I	GGYRCC	1 cut(s)	1429
AccI	GTMKAC	5 cut(s)	318, 324, 366, 1064, 1086
AccIII	TCCGGA	1 cut(s)	462
AclWI	GGATC	9 cut(s)	63, 139, 149, 366, 430, 485, 1070, 1401, 1643
AcoI	YGGCCR	5 cut(s)	25, 133, 550, 756, 1576
AcsI	RAATTY	5 cut(s)	874, 908, 1174, 1259, 1529
AcuI	CTGAAG	1 cut(s)	614
AcvI	CACGTG	1 cut(s)	609
AcyI	GRCGYC	4 cut(s)	321, 384, 472, 789
AfaI	GTAC	4 cut(s)	446, 851, 1616, 1695
AfiI	CCNNNNNNNGG	5 cut(s)	131, 257, 274, 1455, 1490
AflII	CTTAAG	1 cut(s)	1453
AflIII	ACRYGT	1 cut(s)	606
AgsI	TTSAA	1 cut(s)	1368
AjnI	CCWGG	3 cut(s)	396, 1108, 1215
AluBI	AGCT	7 cut(s)	166, 197, 337, 885, 1321, 1508, 1567
AluI	AGCT	7 cut(s)	166, 197, 337, 885, 1321, 1508, 1567
Alw21I	GWGCWC	2 cut(s)	168, 339
Alw26I	GTCTC	2 cut(s)	1279, 1299
AlwI	GGATC	9 cut(s)	63, 139, 149, 366, 430, 485, 1070, 1401, 1643
AlwNI	CAGNNNCTG	1 cut(s)	1115
Ama87I	CYCGRG	2 cut(s)	535, 968
Aor13HI	TCCGGA	1 cut(s)	462
AoxI	GGCC	8 cut(s)	25, 133, 330, 408, 550, 756, 774, 1576
ApeKI	GCWGC	6 cut(s)	292, 345, 655, 869, 892, 1667
ApoI	RAATTY	5 cut(s)	874, 908, 1174, 1259, 1529
ArsI	GACNNNNNNTTYG	4 cut(s)	362, 394, 1112, 1144
Asp700I	GAANNNNTTC	1 cut(s)	665
AspLEI	GCGC	9 cut(s)	98, 140, 179, 230, 350, 451, 585, 731, 904
AspS9I	GGNCC	3 cut(s)	330, 394, 1219
AsuC2I	CCSGG	6 cut(s)	129, 300, 329, 760, 969, 970
AsuHPI	GGTGA	3 cut(s)	214, 529, 1294
AvaI	CYCGRG	2 cut(s)	535, 968
AvaII	GGWCC	2 cut(s)	394, 1219
BaeGI	GKGCMC	1 cut(s)	306
BaeI	ACNNNNGTAYC	2 cut(s)	1606, 1639
BalI	TGGCCA	1 cut(s)	1578
BanI	GGYRCC	1 cut(s)	1429
BanII	GRGCYC	4 cut(s)	168, 339, 591, 1250
BauI	CACGAG	2 cut(s)	161, 305
BbrPI	CACGTG	1 cut(s)	609
BbsI	GAAGAC	1 cut(s)	1437
Bbv12I	GWGCWC	2 cut(s)	168, 339
BbvCI	CCTCAGC	1 cut(s)	671
BbvI	GCAGC	6 cut(s)	279, 332, 642, 856, 904, 1654
BccI	CCATC	3 cut(s)	923, 1457, 1484
BceAI	ACGGC	9 cut(s)	40, 94, 299, 374, 423, 475, 485, 565, 768
BciT130I	CCWGG	3 cut(s)	398, 1110, 1217
BcnI	CCSGG	6 cut(s)	129, 300, 329, 760, 969, 970
BcoDI	GTCTC	2 cut(s)	1279, 1299
BfmI	CTRYAG	1 cut(s)	1403
BfrI	CTTAAG	1 cut(s)	1453
BfuAI	ACCTGC	2 cut(s)	233, 505
BglI	GCCNNNNNGGC	1 cut(s)	301
Bme1390I	CCNGG	9 cut(s)	129, 300, 329, 398, 760, 969, 970, 1110, 1217
Bme18I	GGWCC	2 cut(s)	394, 1219
BmeT110I	CYCGRG	2 cut(s)	535, 968
BmgT120I	GGNCC	3 cut(s)	330, 394, 1219
BmiI	GGNNCC	4 cut(s)	82, 974, 1107, 1431
BmrFI	CCNGG	9 cut(s)	129, 300, 329, 398, 760, 969, 970, 1110, 1217
BmsI	GCATC	7 cut(s)	164, 889, 912, 1029, 1202, 1561, 1618
BoxI	GACNNNNGTC	1 cut(s)	1291
BpiI	GAAGAC	1 cut(s)	1437
BplI	GAGNNNNNCTC	2 cut(s)	1508, 1540
BpmI	CTGGAG	1 cut(s)	1005
Bpu10I	CCTNAGC	1 cut(s)	671
BpuEI	CTTGAG	1 cut(s)	1283
BpuMI	CCSGG	6 cut(s)	129, 300, 329, 760, 969, 970
Bsa29I	ATCGAT	2 cut(s)	480, 1462
BsaAI	YACGTR	2 cut(s)	609, 1585
BsaBI	GATNNNNATC	1 cut(s)	1398
BsaHI	GRCGYC	4 cut(s)	321, 384, 472, 789
BsaJI	CCNNGG	9 cut(s)	127, 231, 299, 390, 491, 547, 572, 968, 976
BsaWI	WCCGGW	1 cut(s)	462
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 397, 427
Bsc4I	CCNNNNNNNGG	5 cut(s)	131, 257, 274, 1455, 1490
Bse1I	ACTGG	2 cut(s)	988, 1101
Bse3DI	GCAATG	4 cut(s)	635, 718, 1159, 1165
Bse8I	GATNNNNATC	1 cut(s)	1398
BseAI	TCCGGA	1 cut(s)	462
BseBI	CCWGG	3 cut(s)	398, 1110, 1217
BseCI	ATCGAT	2 cut(s)	480, 1462
BseDI	CCNNGG	9 cut(s)	127, 231, 299, 390, 491, 547, 572, 968, 976
BseGI	GGATG	5 cut(s)	904, 934, 1217, 1501, 1576
BseJI	GATNNNNATC	1 cut(s)	1398
BseLI	CCNNNNNNNGG	5 cut(s)	131, 257, 274, 1455, 1490
BseMI	GCAATG	4 cut(s)	635, 718, 1159, 1165
BseMII	CTCAG	5 cut(s)	416, 685, 825, 1227, 1539
BseNI	ACTGG	2 cut(s)	988, 1101
BseRI	GAGGAG	2 cut(s)	418, 533
BseSI	GKGCMC	1 cut(s)	306
BseX3I	CGGCCG	3 cut(s)	133, 550, 756
BseXI	GCAGC	6 cut(s)	279, 332, 642, 856, 904, 1654
BseYI	CCCAGC	1 cut(s)	39
BsgI	GTGCAG	2 cut(s)	373, 1653
Bsh1285I	CGRYCG	7 cut(s)	136, 328, 373, 553, 679, 759, 820
BshFI	GGCC	8 cut(s)	27, 135, 332, 410, 552, 758, 776, 1578
BshNI	GGYRCC	1 cut(s)	1429
BshVI	ATCGAT	2 cut(s)	480, 1462
BsiEI	CGRYCG	7 cut(s)	136, 328, 373, 553, 679, 759, 820
BsiHKAI	GWGCWC	2 cut(s)	168, 339
BsiHKCI	CYCGRG	2 cut(s)	535, 968
BslFI	GGGAC	1 cut(s)	591
BslI	CCNNNNNNNGG	5 cut(s)	131, 257, 274, 1455, 1490
BsmAI	GTCTC	2 cut(s)	1279, 1299
BsmFI	GGGAC	1 cut(s)	591
BsmI	GAATGC	1 cut(s)	1360
BsnI	GGCC	8 cut(s)	27, 135, 332, 410, 552, 758, 776, 1578
BsoBI	CYCGRG	2 cut(s)	535, 968
Bsp1286I	GDGCHC	5 cut(s)	168, 306, 339, 591, 1250
Bsp13I	TCCGGA	1 cut(s)	462
Bsp1407I	TGTACA	2 cut(s)	849, 1693
BspANI	GGCC	8 cut(s)	27, 135, 332, 410, 552, 758, 776, 1578
BspCNI	CTCAG	5 cut(s)	415, 684, 824, 1228, 1538
BspDI	ATCGAT	2 cut(s)	480, 1462
BspEI	TCCGGA	1 cut(s)	462
BspHI	TCATGA	2 cut(s)	615, 937
BspLI	GGNNCC	4 cut(s)	82, 974, 1107, 1431
BspMAI	CTGCAG	1 cut(s)	1407
BspMI	ACCTGC	2 cut(s)	233, 505
BspPI	GGATC	9 cut(s)	63, 139, 149, 366, 430, 485, 1070, 1401, 1643
BspQI	GCTCTTC	2 cut(s)	344, 701
BspT107I	GGYRCC	1 cut(s)	1429
BspTI	CTTAAG	1 cut(s)	1453
BsrDI	GCAATG	4 cut(s)	635, 718, 1159, 1165
BsrGI	TGTACA	2 cut(s)	849, 1693
BsrI	ACTGG	2 cut(s)	988, 1101
BssECI	CCNNGG	9 cut(s)	127, 231, 299, 390, 491, 547, 572, 968, 976
BssNI	GRCGYC	4 cut(s)	321, 384, 472, 789
BssSI	CACGAG	2 cut(s)	161, 305
BssT1I	CCWWGG	2 cut(s)	572, 976
Bst2BI	CACGAG	2 cut(s)	161, 305
Bst2UI	CCWGG	3 cut(s)	398, 1110, 1217
Bst4CI	ACNGT	3 cut(s)	854, 1190, 1223
Bst6I	CTCTTC	2 cut(s)	344, 701
BstACI	GRCGYC	4 cut(s)	321, 384, 472, 789
BstAFI	CTTAAG	1 cut(s)	1453
BstAUI	TGTACA	2 cut(s)	849, 1693
BstBAI	YACGTR	2 cut(s)	609, 1585
BstC8I	GCNNGC	8 cut(s)	184, 217, 352, 587, 883, 1271, 1565, 1576
BstDEI	CTNAG	5 cut(s)	402, 671, 811, 1236, 1525
BstDSI	CCRYGG	4 cut(s)	231, 390, 491, 547
BstF5I	GGATG	5 cut(s)	904, 934, 1217, 1501, 1576
BstHHI	GCGC	9 cut(s)	98, 140, 179, 230, 350, 451, 585, 731, 904
BstMAI	GTCTC	2 cut(s)	1279, 1299
BstMCI	CGRYCG	7 cut(s)	136, 328, 373, 553, 679, 759, 820
BstNI	CCWGG	3 cut(s)	398, 1110, 1217
BstPAI	GACNNNNGTC	1 cut(s)	1291
BstSCI	CCNGG	9 cut(s)	127, 298, 327, 396, 758, 967, 968, 1108, 1215
BstSFI	CTRYAG	1 cut(s)	1403
BstSLI	GKGCMC	1 cut(s)	306
BstV1I	GCAGC	6 cut(s)	279, 332, 642, 856, 904, 1654
BstV2I	GAAGAC	1 cut(s)	1437
BstXI	CCANNNNNNTGG	1 cut(s)	983
BstZI	CGGCCG	3 cut(s)	133, 550, 756
Bsu15I	ATCGAT	2 cut(s)	480, 1462
BsuRI	GGCC	8 cut(s)	27, 135, 332, 410, 552, 758, 776, 1578
BsuTUI	ATCGAT	2 cut(s)	480, 1462
BtgI	CCRYGG	4 cut(s)	231, 390, 491, 547
BtsCI	GGATG	5 cut(s)	904, 934, 1217, 1501, 1576
BtsI	GCAGTG	2 cut(s)	238, 1280
BtsIMutI	CAGTG	4 cut(s)	238, 981, 1094, 1280
BveI	ACCTGC	2 cut(s)	233, 505
Cac8I	GCNNGC	8 cut(s)	184, 217, 352, 587, 883, 1271, 1565, 1576
CaiI	CAGNNNCTG	1 cut(s)	1115
CciI	TCATGA	2 cut(s)	615, 937
CciNI	GCGGCCGC	1 cut(s)	133
CfoI	GCGC	9 cut(s)	98, 140, 179, 230, 350, 451, 585, 731, 904
Cfr13I	GGNCC	3 cut(s)	330, 394, 1219
Cfr42I	CCGCGG	1 cut(s)	234
Cfr9I	CCCGGG	1 cut(s)	968
ClaI	ATCGAT	2 cut(s)	480, 1462
CseI	GACGC	5 cut(s)	106, 208, 392, 461, 797
CsiI	ACCWGGT	1 cut(s)	1108
Csp6I	GTAC	4 cut(s)	445, 850, 1615, 1694
CspCI	CAANNNNNGTGG	2 cut(s)	1371, 1406
CviAII	CATG	8 cut(s)	616, 784, 905, 938, 1080, 1171, 1426, 1681
CviQI	GTAC	4 cut(s)	445, 850, 1615, 1694
DdeI	CTNAG	5 cut(s)	402, 671, 811, 1236, 1525
EaeI	YGGCCR	5 cut(s)	25, 133, 550, 756, 1576
EagI	CGGCCG	3 cut(s)	133, 550, 756
Eam1104I	CTCTTC	2 cut(s)	344, 701
EarI	CTCTTC	2 cut(s)	344, 701
EciI	GGCGGA	2 cut(s)	137, 273
Ecl136II	GAGCTC	2 cut(s)	166, 337
EclXI	CGGCCG	3 cut(s)	133, 550, 756
Eco130I	CCWWGG	2 cut(s)	572, 976
Eco24I	GRGCYC	4 cut(s)	168, 339, 591, 1250
Eco47I	GGWCC	2 cut(s)	394, 1219
Eco52I	CGGCCG	3 cut(s)	133, 550, 756
Eco53kI	GAGCTC	2 cut(s)	166, 337
Eco57I	CTGAAG	1 cut(s)	614
Eco72I	CACGTG	1 cut(s)	609
Eco88I	CYCGRG	2 cut(s)	535, 968
EcoICRI	GAGCTC	2 cut(s)	166, 337
EcoRI	GAATTC	2 cut(s)	908, 1174
EcoRII	CCWGG	3 cut(s)	396, 1108, 1215
EcoT14I	CCWWGG	2 cut(s)	572, 976
EcoT38I	GRGCYC	4 cut(s)	168, 339, 591, 1250
ErhI	CCWWGG	2 cut(s)	572, 976
FaeI	CATG	8 cut(s)	619, 787, 908, 941, 1083, 1174, 1429, 1684
FaqI	GGGAC	1 cut(s)	591
FatI	CATG	8 cut(s)	615, 783, 904, 937, 1079, 1170, 1425, 1680
FauI	CCCGC	3 cut(s)	424, 578, 724
FauNDI	CATATG	1 cut(s)	1072
FblI	GTMKAC	5 cut(s)	318, 324, 366, 1064, 1086
FokI	GGATG	5 cut(s)	911, 941, 1224, 1508, 1583
FriOI	GRGCYC	4 cut(s)	168, 339, 591, 1250
FspAI	RTGCGCAY	1 cut(s)	903
FspI	TGCGCA	1 cut(s)	903
GlaI	GCGC	9 cut(s)	97, 139, 178, 229, 349, 450, 584, 730, 903
GsaI	CCCAGC	1 cut(s)	43
GsuI	CTGGAG	1 cut(s)	1005
HaeIII	GGCC	8 cut(s)	27, 135, 332, 410, 552, 758, 776, 1578
HgaI	GACGC	5 cut(s)	106, 208, 392, 461, 797
HhaI	GCGC	9 cut(s)	98, 140, 179, 230, 350, 451, 585, 731, 904
Hin1I	GRCGYC	4 cut(s)	321, 384, 472, 789
Hin1II	CATG	8 cut(s)	619, 787, 908, 941, 1083, 1174, 1429, 1684
Hin6I	GCGC	9 cut(s)	96, 138, 177, 228, 348, 449, 583, 729, 902
HinP1I	GCGC	9 cut(s)	96, 138, 177, 228, 348, 449, 583, 729, 902
HincII	GTYRAC	3 cut(s)	319, 325, 1648
HindII	GTYRAC	3 cut(s)	319, 325, 1648
HindIII	AAGCTT	1 cut(s)	1319
HpaI	GTTAAC	1 cut(s)	1648
HphI	GGTGA	3 cut(s)	214, 529, 1294
Hpy166II	GTNNAC	8 cut(s)	238, 319, 325, 367, 1065, 1087, 1131, 1648
Hpy188I	TCNGA	9 cut(s)	68, 405, 477, 527, 822, 1033, 1252, 1512, 1528
Hpy188III	TCNNGA	4 cut(s)	463, 616, 938, 1179
Hpy8I	GTNNAC	8 cut(s)	238, 319, 325, 367, 1065, 1087, 1131, 1648
Hpy99I	CGWCG	7 cut(s)	10, 320, 323, 326, 386, 477, 527
HpyAV	CCTTC	4 cut(s)	73, 100, 937, 1381
HpyCH4III	ACNGT	3 cut(s)	854, 1190, 1223
HpyCH4IV	ACGT	5 cut(s)	321, 608, 794, 1061, 1584
HpyCH4V	TGCA	9 cut(s)	354, 872, 1118, 1152, 1163, 1170, 1405, 1670, 1739
HpyF3I	CTNAG	5 cut(s)	402, 671, 811, 1236, 1525
HpySE526I	ACGT	5 cut(s)	321, 608, 794, 1061, 1584
Hsp92I	GRCGYC	4 cut(s)	321, 384, 472, 789
Hsp92II	CATG	8 cut(s)	619, 787, 908, 941, 1083, 1174, 1429, 1684
HspAI	GCGC	9 cut(s)	96, 138, 177, 228, 348, 449, 583, 729, 902
Kpn2I	TCCGGA	1 cut(s)	462
KspAI	GTTAAC	1 cut(s)	1648
KspI	CCGCGG	1 cut(s)	234
LguI	GCTCTTC	2 cut(s)	344, 701
LmnI	GCTCC	7 cut(s)	53, 86, 171, 253, 645, 972, 1513
Lsp1109I	GCAGC	6 cut(s)	279, 332, 642, 856, 904, 1654
LweI	GCATC	7 cut(s)	164, 889, 912, 1029, 1202, 1561, 1618
MabI	ACCWGGT	1 cut(s)	1108
MaeII	ACGT	5 cut(s)	321, 608, 794, 1061, 1584
MaeIII	GTNAC	4 cut(s)	220, 779, 1549, 1585
MboII	GAAGA	8 cut(s)	331, 620, 715, 718, 904, 1356, 1437, 1472
MhlI	GDGCHC	5 cut(s)	168, 306, 339, 591, 1250
MlsI	TGGCCA	1 cut(s)	1578
MluCI	AATT	6 cut(s)	595, 874, 908, 1174, 1259, 1529
MluNI	TGGCCA	1 cut(s)	1578
MlyI	GAGTC	5 cut(s)	200, 255, 296, 550, 1301
MmeI	TCCRAC	3 cut(s)	455, 505, 1401
Mox20I	TGGCCA	1 cut(s)	1578
MroI	TCCGGA	1 cut(s)	462
MroXI	GAANNNNTTC	1 cut(s)	665
MscI	TGGCCA	1 cut(s)	1578
MseI	TTAA	3 cut(s)	1454, 1601, 1647
MslI	CAYNNNNRTG	1 cut(s)	920
Msp20I	TGGCCA	1 cut(s)	1578
MspA1I	CMGCKG	2 cut(s)	233, 655
MspCI	CTTAAG	1 cut(s)	1453
MspR9I	CCNGG	9 cut(s)	129, 300, 329, 398, 760, 969, 970, 1110, 1217
Mva1269I	GAATGC	1 cut(s)	1360
MvaI	CCWGG	3 cut(s)	398, 1110, 1217
NciI	CCSGG	6 cut(s)	129, 300, 329, 760, 969, 970
NdeI	CATATG	1 cut(s)	1072
NlaIII	CATG	8 cut(s)	619, 787, 908, 941, 1083, 1174, 1429, 1684
NlaIV	GGNNCC	4 cut(s)	82, 974, 1107, 1431
NmeAIII	GCCGAG	2 cut(s)	310, 358
NmuCI	GTSAC	3 cut(s)	220, 779, 1585
NotI	GCGGCCGC	1 cut(s)	133
NsbI	TGCGCA	1 cut(s)	903
PaeR7I	CTCGAG	1 cut(s)	535
PagI	TCATGA	2 cut(s)	615, 937
PaqCI	CACCTGC	2 cut(s)	233, 505
PciSI	GCTCTTC	2 cut(s)	344, 701
PcsI	WCGNNNNNNNCGW	4 cut(s)	312, 318, 330, 387
PctI	GAATGC	1 cut(s)	1360
PdmI	GAANNNNTTC	1 cut(s)	665
PfeI	GAWTC	5 cut(s)	457, 934, 1141, 1459, 1477
PflFI	GACNNNGTC	1 cut(s)	1126
Ple19I	CGATCG	2 cut(s)	679, 820
PleI	GAGTC	5 cut(s)	199, 255, 295, 550, 1300
PmaCI	CACGTG	1 cut(s)	609
PmlI	CACGTG	1 cut(s)	609
PpsI	GAGTC	5 cut(s)	199, 255, 295, 550, 1300
Ppu21I	YACGTR	2 cut(s)	609, 1585
PshAI	GACNNNNGTC	1 cut(s)	1291
Psp124BI	GAGCTC	2 cut(s)	168, 339
Psp6I	CCWGG	3 cut(s)	396, 1108, 1215
PspCI	CACGTG	1 cut(s)	609
PspFI	CCCAGC	1 cut(s)	39
PspGI	CCWGG	3 cut(s)	396, 1108, 1215
PspN4I	GGNNCC	4 cut(s)	82, 974, 1107, 1431
PspPI	GGNCC	3 cut(s)	330, 394, 1219
PspXI	VCTCGAGB	1 cut(s)	535
PstI	CTGCAG	1 cut(s)	1407
PstNI	CAGNNNCTG	1 cut(s)	1115
PsyI	GACNNNGTC	1 cut(s)	1126
PvuI	CGATCG	2 cut(s)	679, 820
RsaI	GTAC	4 cut(s)	446, 851, 1616, 1695
RsaNI	GTAC	4 cut(s)	445, 850, 1615, 1694
RseI	CAYNNNNRTG	1 cut(s)	920
SacI	GAGCTC	2 cut(s)	168, 339
SacII	CCGCGG	1 cut(s)	234
SalI	GTCGAC	2 cut(s)	317, 323
SapI	GCTCTTC	2 cut(s)	344, 701
SaqAI	TTAA	3 cut(s)	1454, 1601, 1647
Sau96I	GGNCC	3 cut(s)	330, 394, 1219
SchI	GAGTC	5 cut(s)	200, 255, 296, 550, 1301
ScrFI	CCNGG	9 cut(s)	129, 300, 329, 398, 760, 969, 970, 1110, 1217
SduI	GDGCHC	5 cut(s)	168, 306, 339, 591, 1250
SexAI	ACCWGGT	1 cut(s)	1108
SfaNI	GCATC	7 cut(s)	164, 889, 912, 1029, 1202, 1561, 1618
SfcI	CTRYAG	1 cut(s)	1403
Sfr274I	CTCGAG	1 cut(s)	535
Sfr303I	CCGCGG	1 cut(s)	234
SgrBI	CCGCGG	1 cut(s)	234
SgrDI	CGTCGACG	1 cut(s)	317
SinI	GGWCC	2 cut(s)	394, 1219
SlaI	CTCGAG	1 cut(s)	535
SmaI	CCCGGG	1 cut(s)	970
SmiMI	CAYNNNNRTG	1 cut(s)	920
SmlI	CTYRAG	3 cut(s)	535, 1298, 1453
SmoI	CTYRAG	3 cut(s)	535, 1298, 1453
Sse9I	AATT	6 cut(s)	595, 874, 908, 1174, 1259, 1529
SstI	GAGCTC	2 cut(s)	168, 339
StyD4I	CCNGG	9 cut(s)	127, 298, 327, 396, 758, 967, 968, 1108, 1215
StyI	CCWWGG	2 cut(s)	572, 976
TaaI	ACNGT	3 cut(s)	854, 1190, 1223
TaiI	ACGT	5 cut(s)	324, 611, 797, 1064, 1587
TasI	AATT	6 cut(s)	595, 874, 908, 1174, 1259, 1529
TatI	WGTACW	2 cut(s)	849, 1693
TfiI	GAWTC	5 cut(s)	457, 934, 1141, 1459, 1477
Tru1I	TTAA	3 cut(s)	1454, 1601, 1647
Tru9I	TTAA	3 cut(s)	1454, 1601, 1647
TscAI	CASTG	4 cut(s)	245, 988, 1101, 1280
TseFI	GTSAC	3 cut(s)	220, 779, 1585
TseI	GCWGC	6 cut(s)	292, 345, 655, 869, 892, 1667
Tsp45I	GTSAC	3 cut(s)	220, 779, 1585
TspDTI	ATGAA	6 cut(s)	604, 632, 904, 921, 926, 1187
TspMI	CCCGGG	1 cut(s)	968
TspRI	CASTG	4 cut(s)	245, 988, 1101, 1280
Tth111I	GACNNNGTC	1 cut(s)	1126
Vha464I	CTTAAG	1 cut(s)	1453
VpaK11BI	GGWCC	2 cut(s)	394, 1219
XapI	RAATTY	5 cut(s)	874, 908, 1174, 1259, 1529
XhoI	CTCGAG	1 cut(s)	535
XmaI	CCCGGG	1 cut(s)	968
XmiI	GTMKAC	5 cut(s)	318, 324, 366, 1064, 1086
XmnI	GAANNNNTTC	1 cut(s)	665
ZraI	GACGTC	1 cut(s)	322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.