Rmu_co8225392.1_g000001

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8225392.1
OG0009945
Physical Location & Seq
Forward (+)
94 .. 747
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8225392.1_g000001.1.cds

Sequence Viewer

Length: 654 bp
atgtcgtcgatgaccaagatcaacggccacgctctaccccagccctgctcccacctcgccgccttccgatccaaaaacggctccaaacccttccgcgccttacaggactgcctccgcgtcaagccccccggcggccgcgcctcgatccgccgcgatccccacgagctcccccgatgcgccgcctgcggcgagtcagctcactcgcggctctacgcctgcgtcacctgcgccgcggttcactgccacgctccctccggcggactctcccacgcggcggcccacgccgagtcgctgcctccggggcacgagatcgccgtcgacgtcgaccgggccgagctcttctgctgcgcgtgcagggatcaagtctacgaccgcgacttcgacgccgccgtggtcctggctcagacggcctctcccaccaccgcctcctccgcgatccagcagtacgcgccggagaatctccggaaacggcgtcggatcgattacaagccgtggtcgccggatttgagtgagcaggtgatcgtcggaaaaaactcgagcctcctccacggccggactccctccgctttgccttggggactgcgcgggctcaacaatttggggaacacgtgcttcatgaactcggttcttcaggcattgctccacacgccgccg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

218

Amino Acids

23.54

Weight (kDa)

8.94

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 233, 505
AatII GACGTC 1 cut(s) 324
Acc36I ACCTGC 2 cut(s) 233, 505
AccI GTMKAC 3 cut(s) 318, 324, 366
AccIII TCCGGA 1 cut(s) 462
AclWI GGATC 6 cut(s) 63, 139, 149, 366, 430, 485
AcoI YGGCCR 3 cut(s) 25, 133, 550
AcuI CTGAAG 1 cut(s) 614
AcvI CACGTG 1 cut(s) 609
AcyI GRCGYC 3 cut(s) 321, 384, 472
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 3 cut(s) 131, 257, 274
AflIII ACRYGT 1 cut(s) 606
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 3 cut(s) 166, 197, 337
AluI AGCT 3 cut(s) 166, 197, 337
Alw21I GWGCWC 2 cut(s) 168, 339
AlwI GGATC 6 cut(s) 63, 139, 149, 366, 430, 485
Ama87I CYCGRG 1 cut(s) 535
Aor13HI TCCGGA 1 cut(s) 462
AoxI GGCC 6 cut(s) 25, 133, 276, 330, 408, 550
ApeKI GCWGC 2 cut(s) 292, 345
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
AspLEI GCGC 7 cut(s) 98, 140, 179, 230, 350, 451, 585
AspS9I GGNCC 3 cut(s) 277, 330, 394
AsuC2I CCSGG 3 cut(s) 129, 300, 329
AsuHPI GGTGA 2 cut(s) 214, 529
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 1 cut(s) 394
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 3 cut(s) 168, 339, 591
BauI CACGAG 2 cut(s) 161, 305
BbrPI CACGTG 1 cut(s) 609
Bbv12I GWGCWC 2 cut(s) 168, 339
BbvI GCAGC 2 cut(s) 279, 332
BceAI ACGGC 8 cut(s) 40, 94, 299, 374, 423, 475, 485, 565
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 3 cut(s) 129, 300, 329
BfuAI ACCTGC 2 cut(s) 233, 505
BglI GCCNNNNNGGC 1 cut(s) 301
Bme1390I CCNGG 4 cut(s) 129, 300, 329, 398
Bme18I GGWCC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 3 cut(s) 277, 330, 394
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 4 cut(s) 129, 300, 329, 398
BmsI GCATC 1 cut(s) 164
BpuMI CCSGG 3 cut(s) 129, 300, 329
Bsa29I ATCGAT 1 cut(s) 480
BsaAI YACGTR 1 cut(s) 609
BsaHI GRCGYC 3 cut(s) 321, 384, 472
BsaJI CCNNGG 7 cut(s) 127, 231, 299, 390, 491, 547, 572
BsaWI WCCGGW 1 cut(s) 462
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 397, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 257, 274
Bse3DI GCAATG 1 cut(s) 635
BseAI TCCGGA 1 cut(s) 462
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 7 cut(s) 127, 231, 299, 390, 491, 547, 572
BseLI CCNNNNNNNGG 3 cut(s) 131, 257, 274
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 416
BseRI GAGGAG 2 cut(s) 418, 533
BseSI GKGCMC 1 cut(s) 306
BseX3I CGGCCG 2 cut(s) 133, 550
BseXI GCAGC 2 cut(s) 279, 332
BseYI CCCAGC 1 cut(s) 39
BsgI GTGCAG 1 cut(s) 373
Bsh1285I CGRYCG 4 cut(s) 136, 328, 373, 553
BshFI GGCC 6 cut(s) 27, 135, 278, 332, 410, 552
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 4 cut(s) 136, 328, 373, 553
BsiHKAI GWGCWC 2 cut(s) 168, 339
BsiHKCI CYCGRG 1 cut(s) 535
BsiSI CCGG 8 cut(s) 129, 255, 299, 328, 452, 463, 500, 553
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 3 cut(s) 131, 257, 274
BsmFI GGGAC 1 cut(s) 591
BsnI GGCC 6 cut(s) 27, 135, 278, 332, 410, 552
BsoBI CYCGRG 1 cut(s) 535
Bsp1286I GDGCHC 4 cut(s) 168, 306, 339, 591
Bsp13I TCCGGA 1 cut(s) 462
Bsp143I GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
BspANI GGCC 6 cut(s) 27, 135, 278, 332, 410, 552
BspCNI CTCAG 1 cut(s) 415
BspDI ATCGAT 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 462
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 1 cut(s) 82
BspMI ACCTGC 2 cut(s) 233, 505
BspPI GGATC 6 cut(s) 63, 139, 149, 366, 430, 485
BspQI GCTCTTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 635
BssECI CCNNGG 7 cut(s) 127, 231, 299, 390, 491, 547, 572
BssMI GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
BssNI GRCGYC 3 cut(s) 321, 384, 472
BssSI CACGAG 2 cut(s) 161, 305
BssT1I CCWWGG 1 cut(s) 572
Bst2BI CACGAG 2 cut(s) 161, 305
Bst2UI CCWGG 1 cut(s) 398
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 3 cut(s) 321, 384, 472
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 4 cut(s) 184, 217, 352, 587
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 4 cut(s) 231, 390, 491, 547
BstHHI GCGC 7 cut(s) 98, 140, 179, 230, 350, 451, 585
BstKTI GATC 9 cut(s) 21, 71, 147, 157, 312, 361, 438, 480, 522
BstMBI GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
BstMCI CGRYCG 4 cut(s) 136, 328, 373, 553
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 4 cut(s) 127, 298, 327, 396
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 279, 332
BstZI CGGCCG 2 cut(s) 133, 550
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 6 cut(s) 27, 135, 278, 332, 410, 552
BsuTUI ATCGAT 1 cut(s) 480
BtgI CCRYGG 4 cut(s) 231, 390, 491, 547
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 2 cut(s) 233, 505
Cac8I GCNNGC 4 cut(s) 184, 217, 352, 587
CciI TCATGA 1 cut(s) 615
CciNI GCGGCCGC 1 cut(s) 133
CfoI GCGC 7 cut(s) 98, 140, 179, 230, 350, 451, 585
Cfr13I GGNCC 3 cut(s) 277, 330, 394
Cfr42I CCGCGG 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 4 cut(s) 106, 208, 392, 461
Csp6I GTAC 1 cut(s) 445
CviAII CATG 1 cut(s) 616
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 1 cut(s) 402
DpnI GATC 9 cut(s) 20, 70, 146, 156, 311, 360, 437, 479, 521
DpnII GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
EaeI YGGCCR 3 cut(s) 25, 133, 550
EagI CGGCCG 2 cut(s) 133, 550
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 2 cut(s) 137, 273
Ecl136II GAGCTC 2 cut(s) 166, 337
EclXI CGGCCG 2 cut(s) 133, 550
Eco130I CCWWGG 1 cut(s) 572
Eco24I GRGCYC 3 cut(s) 168, 339, 591
Eco47I GGWCC 1 cut(s) 394
Eco52I CGGCCG 2 cut(s) 133, 550
Eco53kI GAGCTC 2 cut(s) 166, 337
Eco57I CTGAAG 1 cut(s) 614
Eco72I CACGTG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 535
EcoICRI GAGCTC 2 cut(s) 166, 337
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 3 cut(s) 168, 339, 591
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 1 cut(s) 619
FaiI YATR 1 cut(s) 617
FaqI GGGAC 1 cut(s) 591
FatI CATG 1 cut(s) 615
FauI CCCGC 1 cut(s) 578
FblI GTMKAC 3 cut(s) 318, 324, 366
FriOI GRGCYC 3 cut(s) 168, 339, 591
GlaI GCGC 7 cut(s) 97, 139, 178, 229, 349, 450, 584
GsaI CCCAGC 1 cut(s) 43
HaeIII GGCC 6 cut(s) 27, 135, 278, 332, 410, 552
HapII CCGG 8 cut(s) 129, 255, 299, 328, 452, 463, 500, 553
HgaI GACGC 4 cut(s) 106, 208, 392, 461
HhaI GCGC 7 cut(s) 98, 140, 179, 230, 350, 451, 585
Hin1I GRCGYC 3 cut(s) 321, 384, 472
Hin1II CATG 1 cut(s) 619
Hin6I GCGC 7 cut(s) 96, 138, 177, 228, 348, 449, 583
HinP1I GCGC 7 cut(s) 96, 138, 177, 228, 348, 449, 583
HincII GTYRAC 2 cut(s) 319, 325
HindII GTYRAC 2 cut(s) 319, 325
HinfI GANTC 5 cut(s) 191, 261, 287, 457, 556
HpaII CCGG 8 cut(s) 129, 255, 299, 328, 452, 463, 500, 553
HphI GGTGA 2 cut(s) 214, 529
Hpy166II GTNNAC 4 cut(s) 238, 319, 325, 367
Hpy188I TCNGA 4 cut(s) 68, 405, 477, 527
Hpy188III TCNNGA 2 cut(s) 463, 616
Hpy8I GTNNAC 4 cut(s) 238, 319, 325, 367
Hpy99I CGWCG 7 cut(s) 10, 320, 323, 326, 386, 477, 527
HpyAV CCTTC 2 cut(s) 73, 100
HpyCH4IV ACGT 2 cut(s) 321, 608
HpyCH4V TGCA 1 cut(s) 354
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 2 cut(s) 321, 608
Hsp92I GRCGYC 3 cut(s) 321, 384, 472
Hsp92II CATG 1 cut(s) 619
HspAI GCGC 7 cut(s) 96, 138, 177, 228, 348, 449, 583
Kpn2I TCCGGA 1 cut(s) 462
KspI CCGCGG 1 cut(s) 234
Kzo9I GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 5 cut(s) 53, 86, 171, 253, 645
Lsp1109I GCAGC 2 cut(s) 279, 332
LweI GCATC 1 cut(s) 164
MaeII ACGT 2 cut(s) 321, 608
MaeIII GTNAC 1 cut(s) 220
MalI GATC 9 cut(s) 20, 70, 146, 156, 311, 360, 437, 479, 521
MboI GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
MboII GAAGA 2 cut(s) 331, 620
MhlI GDGCHC 4 cut(s) 168, 306, 339, 591
MluCI AATT 1 cut(s) 595
MlyI GAGTC 4 cut(s) 200, 255, 296, 550
MmeI TCCRAC 2 cut(s) 455, 505
MroI TCCGGA 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 8 cut(s) 129, 255, 299, 328, 452, 463, 500, 553
MspR9I CCNGG 4 cut(s) 129, 300, 329, 398
MvaI CCWGG 1 cut(s) 398
NciI CCSGG 3 cut(s) 129, 300, 329
NdeII GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 1 cut(s) 82
NmeAIII GCCGAG 2 cut(s) 310, 358
NmuCI GTSAC 1 cut(s) 220
NotI GCGGCCGC 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 535
PagI TCATGA 1 cut(s) 615
PaqCI CACCTGC 2 cut(s) 233, 505
PciSI GCTCTTC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 4 cut(s) 312, 318, 330, 387
PfeI GAWTC 1 cut(s) 457
PleI GAGTC 4 cut(s) 199, 255, 295, 550
PmaCI CACGTG 1 cut(s) 609
PmlI CACGTG 1 cut(s) 609
PpsI GAGTC 4 cut(s) 199, 255, 295, 550
Ppu21I YACGTR 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 168, 339
Psp6I CCWGG 1 cut(s) 396
PspCI CACGTG 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 82
PspPI GGNCC 3 cut(s) 277, 330, 394
PspXI VCTCGAGB 1 cut(s) 535
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SacI GAGCTC 2 cut(s) 168, 339
SacII CCGCGG 1 cut(s) 234
SalI GTCGAC 2 cut(s) 317, 323
SapI GCTCTTC 1 cut(s) 344
Sau3AI GATC 9 cut(s) 18, 68, 144, 154, 309, 358, 435, 477, 519
Sau96I GGNCC 3 cut(s) 277, 330, 394
SchI GAGTC 4 cut(s) 200, 255, 296, 550
ScrFI CCNGG 4 cut(s) 129, 300, 329, 398
SduI GDGCHC 4 cut(s) 168, 306, 339, 591
SetI ASST 8 cut(s) 57, 168, 199, 227, 324, 339, 519, 611
SfaNI GCATC 1 cut(s) 164
Sfr274I CTCGAG 1 cut(s) 535
Sfr303I CCGCGG 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 234
SgrDI CGTCGACG 1 cut(s) 317
SinI GGWCC 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 535
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
Sse9I AATT 1 cut(s) 595
SstI GAGCTC 2 cut(s) 168, 339
StyD4I CCNGG 4 cut(s) 127, 298, 327, 396
StyI CCWWGG 1 cut(s) 572
TaiI ACGT 2 cut(s) 324, 611
TaqI TCGA 7 cut(s) 8, 143, 318, 324, 381, 480, 536
TasI AATT 1 cut(s) 595
TfiI GAWTC 1 cut(s) 457
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 2 cut(s) 292, 345
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 604, 632
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 394
XhoI CTCGAG 1 cut(s) 535
XmiI GTMKAC 3 cut(s) 318, 324, 366
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.