Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009945

Physical Location & Seq

Genomic Coordinates Reverse (-)

12290592 .. 12294112

Gene Structure 3521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1163000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGTCGTCGATGGCCAAGATCACTCACCCCCGCCAACAGATCAACGGCCAGATTCTGCCTCAGCCCTGTCCTCACCTTGCCGAGTTCCTCTCCAACAATGGCTCCAAGCCCTTCCGGGCCTTGCAGGACTGTCTACGGGTCAAGCCGCCGGGTGGCCGCGCATCTATCCGCCGCGATCCCAAGGAGGTCCCGCGATGTGGCGCGTGCGGGGAGTCCGAGCGTCCCAGACTCTATGCGTGCATCACGTGCGCTGCCGTCCACTGTCACGTGCCACCCGGACCATCCCACGCTGCGGCTCACGCCTTCTCGATGCCTCCCGGTCACGAGATCGCCGTGGACGTTGACCGAGCGGAGCTCTTCTGCTGCGCGTGTAGAGACCAAGTCTACGATCGCGACTTCGACGCCGCAGTCGTCCTTGCTCAGACCGCCGCTTCGACTCTGGCCTCCTCAGCCACGTCAGCAGTCCAGCAGTACGCGCCGGAGAATCTCCGGAAGCGACGCCGTATCGACTACAAGCCGTGGATACCGAATCTGAGAGAGCAATACTTGGTGGGGAAGAATTCCAGTCCCCTAAACGGCGACGTCTCCGATTTGCCTTGGGGATTGCGCGGGCTCAACAATTTGGGGAACACGTGCTTTATGAACTCGGTTCTGCAGGCATTGCTCCATACGCCGCCGTTGCGGAACTACTTCCTCAGCGATCGGCATAACCGCTACTTCTGCCAGAAGAAGAGCAATGCCGATAACGCCGCCAAGAAAACTGCTGCCAATACTATTGATGGTGGCGGGAACAAGAGCGCTGCGCGCGTGTGCTTGGCCTGCGACATGGACGCCACGTTTTCTGCGGTTTTTTCGGGGGAGCGGACGCCTTATAGCCCCGCAAAGTTCTTGTACAGTTGGTGGCAGTACGCCGCGAATTTGGCGAGTTATGAGCAGCAGGACGCGCATGAGTTCTTCATTTCTATGCTTGATGGGATTCATGAGAAGGTGGATAAGGATCGGCGGAAACCCGAGAGCCAAGGCAATGGAGACTGTTGTGTTGCTCACAGAGTTTTTTCTGGTATCTTGCGATCTGATGTCATGTGTATGGCCTGTGGTTTTACATCTACAACATATGACCCATGTGTGGACATCTCATTAGATTTGGAACCTAACCAAGGAGGTTCTGCAAAGACTGGGTCCACAAAGTCAAGTCATTCTTGCAATGGCGAGGCAGATTGCATGAATTCCAGCCAAAACTGTGGGGTTTCTACCCTTATGGGATGCTTGGACAGCTTTACGAGACCTGAGAGATTGGGCGCTGACCAGAAATTTTTTTGCCAGCAGTGTCAGGTGAGGCAGGAGTCTCTCAAGCAAATGTCCATAAGAAAGCTTCCTTTGGTTTCATGCTTCCACATCAAGCGATTTGAACATTCTTCGGTGAGGAAAATGTCAAGGAAGGTTGACCGTTATTTGCAGTTCCCATTTTCGCTTGACATGGCTCCTTATCTTTCTTCTTCCATCTTGAGAAGCCGATTTGGAAATAGGATTTTGCCTTTTGCTGGGGACAAGCCGGATGCATCAAATGACTTATGTTCAGAGTTTGAGTTGTTTGCTGTCGTCACTCACACAGGCAAGCTAGATGCAGGCCATTATGTGACTTACTTGCGATTGAGTAATCAGTGGTACAAGTGTGACGATGCTTGGATCACACAAGTTAATGAGAACATTGTGAGGGCAGCACAGGGCTACATGATGTTCTATGTACAGAAAATGCTATTTTACAAGGCAAGTGAGAAACCGAGTCCTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-UBP

Protein Analysis

598

Amino Acids

66.54

Weight (kDa)

8.66

Isoelectric Point (pI)

51.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	66 - 132	1.8e-09	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	203 - 581	1e-58	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	204 - 560	3.1e-16	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009945)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02230
malus_domestica	MD02G1020200.v1.1 MD15G1163000.v1.1
prunus_persica	Prupe.7G251600_v2.0.a1
pyrus_communis	pycom15g14660
rosa_chinensis	RchiOBHm_Chr2g0087351 RchiOBHm_Chr2g0087361
rosa_laevigata	RLG00000015859
rosa_multiflora	Rmu_co8225392.1_g000001 Rmu_co8488985.1_g000001 Rmu_sc0003367.1_g000026 Rmu_sc0006064.1_g000003 Rmu_sc0033647.1_g000002
rosa_roxburghii	Rroxscaffold_2G00153670
rosa_rugosa	Rorug01G0474400
rosa_samantha	Rh2AG028000 Rh2AG028100 Rh2BG027500 Rh2CG028300 Rh2DG028100
rosa_wichuraiana	Rw2G002220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	407
AatII	GACGTC	1 cut(s)	585
AccBSI	CCGCTC	2 cut(s)	350, 862
AccI	GTMKAC	2 cut(s)	133, 384
AccIII	TCCGGA	1 cut(s)	489
AclWI	GGATC	3 cut(s)	170, 1005, 1694
AcoI	YGGCCR	3 cut(s)	12, 46, 154
AcsI	RAATTY	4 cut(s)	559, 916, 1225, 1310
AcvI	CACGTG	3 cut(s)	246, 268, 633
AcyI	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
AfaI	GTAC	5 cut(s)	473, 893, 908, 1667, 1746
AfeI	AGCGCT	1 cut(s)	799
AfiI	CCNNNNNNNGG	7 cut(s)	152, 197, 292, 575, 1126, 1157, 1541
AflIII	ACRYGT	1 cut(s)	630
AgsI	TTSAA	1 cut(s)	1409
AjiI	CACGTC	1 cut(s)	456
AluBI	AGCT	4 cut(s)	355, 1275, 1372, 1618
AluI	AGCT	4 cut(s)	355, 1275, 1372, 1618
Alw21I	GWGCWC	1 cut(s)	357
Alw26I	GTCTC	5 cut(s)	369, 589, 1023, 1276, 1350
AlwI	GGATC	3 cut(s)	170, 1005, 1694
AlwNI	CAGNNNCTG	1 cut(s)	55
Ama87I	CYCGRG	1 cut(s)	1010
Aor13HI	TCCGGA	1 cut(s)	489
Aor51HI	AGCGCT	1 cut(s)	799
AoxI	GGCC	8 cut(s)	12, 46, 117, 154, 441, 816, 1089, 1627
ApeKI	GCWGC	7 cut(s)	251, 290, 363, 764, 800, 934, 1718
ApoI	RAATTY	4 cut(s)	559, 916, 1225, 1310
ArsI	GACNNNNNNTTYG	4 cut(s)	415, 447, 1163, 1195
Asp700I	GAANNNNTTC	1 cut(s)	689
AspS9I	GGNCC	4 cut(s)	117, 187, 278, 1179
AsuC2I	CCSGG	4 cut(s)	116, 150, 276, 318
AsuHPI	GGTGA	4 cut(s)	17, 65, 1345, 1432
AvaI	CYCGRG	1 cut(s)	1010
AvaII	GGWCC	3 cut(s)	187, 278, 1179
BaeI	ACNNNNGTAYC	2 cut(s)	1657, 1690
BalI	TGGCCA	1 cut(s)	14
BanII	GRGCYC	2 cut(s)	357, 615
BauI	CACGAG	1 cut(s)	323
BbrPI	CACGTG	3 cut(s)	246, 268, 633
Bbv12I	GWGCWC	1 cut(s)	357
BbvCI	CCTCAGC	3 cut(s)	60, 448, 695
BbvI	GCAGC	7 cut(s)	238, 277, 350, 751, 787, 946, 1730
BccI	CCATC	5 cut(s)	4, 289, 773, 965, 1508
BceAI	ACGGC	7 cut(s)	61, 239, 317, 486, 502, 592, 661
BciVI	GTATCC	1 cut(s)	516
BcnI	CCSGG	4 cut(s)	116, 150, 276, 318
BcoDI	GTCTC	5 cut(s)	369, 589, 1023, 1276, 1350
BfaI	CTAG	1 cut(s)	1619
BfmI	CTRYAG	1 cut(s)	653
BfoI	RGCGCY	2 cut(s)	801, 1302
BfuI	GTATCC	1 cut(s)	516
Bme1390I	CCNGG	4 cut(s)	116, 150, 276, 318
Bme18I	GGWCC	3 cut(s)	187, 278, 1179
BmeT110I	CYCGRG	1 cut(s)	1010
BmgBI	CACGTC	1 cut(s)	456
BmgT120I	GGNCC	4 cut(s)	117, 187, 278, 1179
BmiI	GGNNCC	5 cut(s)	103, 189, 1149, 1180, 1482
BmrFI	CCNGG	4 cut(s)	116, 150, 276, 318
BmrI	ACTGGG	1 cut(s)	1185
BmsI	GCATC	8 cut(s)	170, 249, 300, 1253, 1546, 1568, 1612, 1669
BmuI	ACTGGG	1 cut(s)	1185
BplI	GAGNNNNNCTC	4 cut(s)	74, 106, 339, 371
Bpu10I	CCTNAGC	3 cut(s)	60, 448, 695
BpuEI	CTTGAG	2 cut(s)	1334, 1525
BpuMI	CCSGG	4 cut(s)	116, 150, 276, 318
BsaAI	YACGTR	3 cut(s)	246, 268, 633
BsaBI	GATNNNNATC	1 cut(s)	996
BsaHI	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
BsaI	GGTCTC	2 cut(s)	369, 1276
BsaJI	CCNNGG	6 cut(s)	180, 333, 518, 596, 1018, 1156
BsaWI	WCCGGW	1 cut(s)	489
BsaXI	ACNNNNNCTCC	2 cut(s)	86, 116
Bsc4I	CCNNNNNNNGG	7 cut(s)	152, 197, 292, 575, 1126, 1157, 1541
Bse1I	ACTGG	2 cut(s)	564, 1180
Bse3DI	GCAATG	4 cut(s)	659, 742, 1030, 1210
Bse8I	GATNNNNATC	1 cut(s)	996
BseAI	TCCGGA	1 cut(s)	489
BseDI	CCNNGG	6 cut(s)	180, 333, 518, 596, 1018, 1156
BseGI	GGATG	3 cut(s)	281, 1268, 1561
BseJI	GATNNNNATC	1 cut(s)	996
BseLI	CCNNNNNNNGG	7 cut(s)	152, 197, 292, 575, 1126, 1157, 1541
BseMI	GCAATG	4 cut(s)	659, 742, 1030, 1210
BseMII	CTCAG	6 cut(s)	74, 434, 462, 524, 709, 1278
BseNI	ACTGG	2 cut(s)	564, 1180
BsePI	GCGCGC	1 cut(s)	803
BseRI	GAGGAG	1 cut(s)	436
BseXI	GCAGC	7 cut(s)	238, 277, 350, 751, 787, 946, 1730
BseYI	CCCAGC	1 cut(s)	1541
Bsh1285I	CGRYCG	2 cut(s)	391, 703
BshFI	GGCC	8 cut(s)	14, 48, 119, 156, 443, 818, 1091, 1629
BsiEI	CGRYCG	2 cut(s)	391, 703
BsiHKAI	GWGCWC	1 cut(s)	357
BsiHKCI	CYCGRG	1 cut(s)	1010
BsiSI	CCGG	7 cut(s)	115, 149, 276, 318, 479, 490, 1553
BslFI	GGGAC	4 cut(s)	173, 207, 552, 1559
BslI	CCNNNNNNNGG	7 cut(s)	152, 197, 292, 575, 1126, 1157, 1541
BsmAI	GTCTC	5 cut(s)	369, 589, 1023, 1276, 1350
BsmBI	CGTCTC	1 cut(s)	589
BsmFI	GGGAC	4 cut(s)	173, 207, 552, 1559
BsnI	GGCC	8 cut(s)	14, 48, 119, 156, 443, 818, 1091, 1629
Bso31I	GGTCTC	2 cut(s)	369, 1276
BsoBI	CYCGRG	1 cut(s)	1010
Bsp1286I	GDGCHC	2 cut(s)	357, 615
Bsp13I	TCCGGA	1 cut(s)	489
Bsp1407I	TGTACA	2 cut(s)	891, 1744
Bsp143I	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
Bsp68I	TCGCGA	1 cut(s)	393
BspANI	GGCC	8 cut(s)	14, 48, 119, 156, 443, 818, 1091, 1629
BspCNI	CTCAG	6 cut(s)	73, 433, 461, 525, 708, 1279
BspEI	TCCGGA	1 cut(s)	489
BspHI	TCATGA	1 cut(s)	979
BspLI	GGNNCC	5 cut(s)	103, 189, 1149, 1180, 1482
BspMAI	CTGCAG	1 cut(s)	657
BspPI	GGATC	3 cut(s)	170, 1005, 1694
BspQI	GCTCTTC	2 cut(s)	362, 725
BspTNI	GGTCTC	2 cut(s)	369, 1276
BsrBI	CCGCTC	2 cut(s)	350, 862
BsrDI	GCAATG	4 cut(s)	659, 742, 1030, 1210
BsrGI	TGTACA	2 cut(s)	891, 1744
BsrI	ACTGG	2 cut(s)	564, 1180
BssECI	CCNNGG	6 cut(s)	180, 333, 518, 596, 1018, 1156
BssHII	GCGCGC	1 cut(s)	803
BssMI	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
BssNI	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
BssSI	CACGAG	1 cut(s)	323
BssT1I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
Bst2BI	CACGAG	1 cut(s)	323
Bst4CI	ACNGT	6 cut(s)	131, 263, 896, 1034, 1241, 1448
Bst6I	CTCTTC	2 cut(s)	362, 725
BstACI	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
BstAPI	GCANNNNNTGC	2 cut(s)	246, 661
BstAUI	TGTACA	2 cut(s)	891, 1744
BstBAI	YACGTR	3 cut(s)	246, 268, 633
BstC8I	GCNNGC	9 cut(s)	205, 238, 611, 657, 805, 820, 1322, 1616, 1627
BstDEI	CTNAG	6 cut(s)	60, 420, 448, 533, 695, 1287
BstDSI	CCRYGG	2 cut(s)	333, 518
BstENI	CCTNNNNNAGG	1 cut(s)	1155
BstF5I	GGATG	3 cut(s)	281, 1268, 1561
BstH2I	RGCGCY	2 cut(s)	801, 1302
BstKTI	GATC	9 cut(s)	21, 42, 178, 330, 391, 703, 1000, 1073, 1689
BstMAI	GTCTC	5 cut(s)	369, 589, 1023, 1276, 1350
BstMBI	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
BstMCI	CGRYCG	2 cut(s)	391, 703
BstSCI	CCNGG	4 cut(s)	114, 148, 274, 316
BstSFI	CTRYAG	1 cut(s)	653
BstV1I	GCAGC	7 cut(s)	238, 277, 350, 751, 787, 946, 1730
BstXI	CCANNNNNNTGG	2 cut(s)	1025, 1241
BsuI	GTATCC	1 cut(s)	516
BsuRI	GGCC	8 cut(s)	14, 48, 119, 156, 443, 818, 1091, 1629
BtgI	CCRYGG	2 cut(s)	333, 518
BtgZI	GCGATG	1 cut(s)	208
BtrI	CACGTC	1 cut(s)	456
BtsCI	GGATG	3 cut(s)	281, 1268, 1561
BtsI	GCAGTG	1 cut(s)	1331
BtsIMutI	CAGTG	3 cut(s)	259, 1331, 1667
BtuMI	TCGCGA	1 cut(s)	393
Cac8I	GCNNGC	9 cut(s)	205, 238, 611, 657, 805, 820, 1322, 1616, 1627
CaiI	CAGNNNCTG	1 cut(s)	55
CciI	TCATGA	1 cut(s)	979
Cfr13I	GGNCC	4 cut(s)	117, 187, 278, 1179
CseI	GACGC	6 cut(s)	209, 410, 507, 839, 874, 950
Csp6I	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
CviQI	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
DdeI	CTNAG	6 cut(s)	60, 420, 448, 533, 695, 1287
DpnI	GATC	9 cut(s)	20, 41, 177, 329, 390, 702, 999, 1072, 1688
DpnII	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
DrdI	GACNNNNNNGTC	1 cut(s)	407
DseDI	GACNNNNNNGTC	1 cut(s)	407
EaeI	YGGCCR	3 cut(s)	12, 46, 154
Eam1104I	CTCTTC	2 cut(s)	362, 725
EarI	CTCTTC	2 cut(s)	362, 725
EciI	GGCGGA	2 cut(s)	158, 1018
Ecl136II	GAGCTC	1 cut(s)	355
Eco130I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
Eco24I	GRGCYC	2 cut(s)	357, 615
Eco31I	GGTCTC	2 cut(s)	369, 1276
Eco47I	GGWCC	3 cut(s)	187, 278, 1179
Eco47III	AGCGCT	1 cut(s)	799
Eco53kI	GAGCTC	1 cut(s)	355
Eco72I	CACGTG	3 cut(s)	246, 268, 633
Eco88I	CYCGRG	1 cut(s)	1010
EcoICRI	GAGCTC	1 cut(s)	355
EcoNI	CCTNNNNNAGG	1 cut(s)	1155
EcoO109I	RGGNCCY	1 cut(s)	187
EcoRI	GAATTC	2 cut(s)	559, 1225
EcoT14I	CCWWGG	4 cut(s)	180, 596, 1018, 1156
EcoT22I	ATGCAT	1 cut(s)	1561
EcoT38I	GRGCYC	2 cut(s)	357, 615
ErhI	CCWWGG	4 cut(s)	180, 596, 1018, 1156
Esp3I	CGTCTC	1 cut(s)	589
FalI	AAGNNNNNCTT	2 cut(s)	1183, 1215
FaqI	GGGAC	4 cut(s)	173, 207, 552, 1559
FauI	CCCGC	6 cut(s)	38, 198, 200, 602, 779, 886
FauNDI	CATATG	1 cut(s)	1114
FblI	GTMKAC	2 cut(s)	133, 384
FokI	GGATG	3 cut(s)	268, 1275, 1568
FriOI	GRGCYC	2 cut(s)	357, 615
FspBI	CTAG	1 cut(s)	1619
GsaI	CCCAGC	1 cut(s)	1545
HaeII	RGCGCY	2 cut(s)	801, 1302
HaeIII	GGCC	8 cut(s)	14, 48, 119, 156, 443, 818, 1091, 1629
HapII	CCGG	7 cut(s)	115, 149, 276, 318, 479, 490, 1553
HgaI	GACGC	6 cut(s)	209, 410, 507, 839, 874, 950
Hin1I	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
HincII	GTYRAC	2 cut(s)	343, 1444
HindII	GTYRAC	2 cut(s)	343, 1444
HindIII	AAGCTT	1 cut(s)	1370
HinfI	GANTC	9 cut(s)	52, 212, 228, 436, 484, 529, 976, 1343, 1783
HpaII	CCGG	7 cut(s)	115, 149, 276, 318, 479, 490, 1553
HphI	GGTGA	4 cut(s)	17, 65, 1345, 1432
Hpy166II	GTNNAC	8 cut(s)	134, 259, 337, 343, 385, 1129, 1182, 1444
Hpy188I	TCNGA	6 cut(s)	217, 423, 534, 589, 1075, 1579
Hpy188III	TCNNGA	6 cut(s)	307, 323, 392, 490, 980, 1504
Hpy8I	GTNNAC	8 cut(s)	134, 259, 337, 343, 385, 1129, 1182, 1444
Hpy99I	CGWCG	4 cut(s)	10, 404, 501, 584
HpyAV	CCTTC	4 cut(s)	121, 313, 979, 1432
HpyCH4III	ACNGT	6 cut(s)	131, 263, 896, 1034, 1241, 1448
HpyCH4IV	ACGT	7 cut(s)	245, 267, 339, 455, 582, 632, 836
HpyCH4V	TGCA	9 cut(s)	124, 240, 655, 1169, 1203, 1221, 1456, 1559, 1625
HpyF3I	CTNAG	6 cut(s)	60, 420, 448, 533, 695, 1287
HpySE526I	ACGT	7 cut(s)	245, 267, 339, 455, 582, 632, 836
Hsp92I	GRCGYC	5 cut(s)	402, 499, 582, 831, 866
Kpn2I	TCCGGA	1 cut(s)	489
Kzo9I	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
LguI	GCTCTTC	2 cut(s)	362, 725
LmnI	GCTCC	5 cut(s)	107, 352, 669, 859, 1486
Lsp1109I	GCAGC	7 cut(s)	238, 277, 350, 751, 787, 946, 1730
LweI	GCATC	8 cut(s)	170, 249, 300, 1253, 1546, 1568, 1612, 1669
MaeI	CTAG	1 cut(s)	1619
MaeII	ACGT	7 cut(s)	245, 267, 339, 455, 582, 632, 836
MaeIII	GTNAC	5 cut(s)	263, 320, 1600, 1636, 1673
MalI	GATC	9 cut(s)	20, 41, 177, 329, 390, 702, 999, 1072, 1688
MbiI	CCGCTC	2 cut(s)	350, 862
MboI	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
MboII	GAAGA	8 cut(s)	349, 568, 739, 742, 946, 1407, 1485, 1488
MhlI	GDGCHC	2 cut(s)	357, 615
MlsI	TGGCCA	1 cut(s)	14
MluCI	AATT	5 cut(s)	559, 619, 916, 1225, 1310
MluNI	TGGCCA	1 cut(s)	14
MlyI	GAGTC	5 cut(s)	221, 222, 430, 1352, 1792
MmeI	TCCRAC	1 cut(s)	117
Mox20I	TGGCCA	1 cut(s)	14
Mph1103I	ATGCAT	1 cut(s)	1561
MroI	TCCGGA	1 cut(s)	489
MroXI	GAANNNNTTC	1 cut(s)	689
MscI	TGGCCA	1 cut(s)	14
MseI	TTAA	1 cut(s)	1698
MslI	CAYNNNNRTG	2 cut(s)	962, 1085
Msp20I	TGGCCA	1 cut(s)	14
MspI	CCGG	7 cut(s)	115, 149, 276, 318, 479, 490, 1553
MspR9I	CCNGG	4 cut(s)	116, 150, 276, 318
MteI	GCGCNGCGC	1 cut(s)	801
NciI	CCSGG	4 cut(s)	116, 150, 276, 318
NdeI	CATATG	1 cut(s)	1114
NdeII	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
NlaIV	GGNNCC	5 cut(s)	103, 189, 1149, 1180, 1482
NmeAIII	GCCGAG	1 cut(s)	106
NmuCI	GTSAC	5 cut(s)	263, 320, 1600, 1636, 1673
NruI	TCGCGA	1 cut(s)	393
NsiI	ATGCAT	1 cut(s)	1561
PagI	TCATGA	1 cut(s)	979
PauI	GCGCGC	1 cut(s)	803
PciSI	GCTCTTC	2 cut(s)	362, 725
PcsI	WCGNNNNNNNCGW	3 cut(s)	330, 336, 408
PdmI	GAANNNNTTC	1 cut(s)	689
PfeI	GAWTC	4 cut(s)	52, 484, 529, 976
PflFI	GACNNNGTC	2 cut(s)	380, 1177
Ple19I	CGATCG	2 cut(s)	391, 703
PleI	GAGTC	5 cut(s)	220, 222, 430, 1351, 1791
PmaCI	CACGTG	3 cut(s)	246, 268, 633
PmlI	CACGTG	3 cut(s)	246, 268, 633
PpsI	GAGTC	5 cut(s)	220, 222, 430, 1351, 1791
Ppu21I	YACGTR	3 cut(s)	246, 268, 633
PpuMI	RGGWCCY	1 cut(s)	187
Psp124BI	GAGCTC	1 cut(s)	357
Psp5II	RGGWCCY	1 cut(s)	187
PspCI	CACGTG	3 cut(s)	246, 268, 633
PspFI	CCCAGC	1 cut(s)	1541
PspN4I	GGNNCC	5 cut(s)	103, 189, 1149, 1180, 1482
PspPI	GGNCC	4 cut(s)	117, 187, 278, 1179
PspPPI	RGGWCCY	1 cut(s)	187
PstI	CTGCAG	1 cut(s)	657
PstNI	CAGNNNCTG	1 cut(s)	55
PsyI	GACNNNGTC	2 cut(s)	380, 1177
PteI	GCGCGC	1 cut(s)	803
PvuI	CGATCG	2 cut(s)	391, 703
RruI	TCGCGA	1 cut(s)	393
RsaI	GTAC	5 cut(s)	473, 893, 908, 1667, 1746
RsaNI	GTAC	5 cut(s)	472, 892, 907, 1666, 1745
RseI	CAYNNNNRTG	2 cut(s)	962, 1085
SacI	GAGCTC	1 cut(s)	357
SapI	GCTCTTC	2 cut(s)	362, 725
SaqAI	TTAA	1 cut(s)	1698
Sau3AI	GATC	9 cut(s)	18, 39, 175, 327, 388, 700, 997, 1070, 1686
Sau96I	GGNCC	4 cut(s)	117, 187, 278, 1179
SchI	GAGTC	5 cut(s)	221, 222, 430, 1352, 1792
ScrFI	CCNGG	4 cut(s)	116, 150, 276, 318
SduI	GDGCHC	2 cut(s)	357, 615
SfaNI	GCATC	8 cut(s)	170, 249, 300, 1253, 1546, 1568, 1612, 1669
SfcI	CTRYAG	1 cut(s)	653
SinI	GGWCC	3 cut(s)	187, 278, 1179
SmiMI	CAYNNNNRTG	2 cut(s)	962, 1085
SmlI	CTYRAG	2 cut(s)	1349, 1504
SmoI	CTYRAG	2 cut(s)	1349, 1504
Sse9I	AATT	5 cut(s)	559, 619, 916, 1225, 1310
SspMI	CTAG	1 cut(s)	1619
SstI	GAGCTC	1 cut(s)	357
StyD4I	CCNGG	4 cut(s)	114, 148, 274, 316
StyI	CCWWGG	4 cut(s)	180, 596, 1018, 1156
TaaI	ACNGT	6 cut(s)	131, 263, 896, 1034, 1241, 1448
TaiI	ACGT	7 cut(s)	248, 270, 342, 458, 585, 635, 839
TaqI	TCGA	5 cut(s)	8, 308, 399, 434, 507
TaqII	GACCGA	1 cut(s)	360
TasI	AATT	5 cut(s)	559, 619, 916, 1225, 1310
TatI	WGTACW	2 cut(s)	891, 1744
TauI	GCSGC	9 cut(s)	148, 159, 174, 296, 407, 431, 676, 752, 914
TfiI	GAWTC	4 cut(s)	52, 484, 529, 976
Tru1I	TTAA	1 cut(s)	1698
Tru9I	TTAA	1 cut(s)	1698
TscAI	CASTG	3 cut(s)	266, 1331, 1667
TseFI	GTSAC	5 cut(s)	263, 320, 1600, 1636, 1673
TseI	GCWGC	7 cut(s)	251, 290, 363, 764, 800, 934, 1718
Tsp45I	GTSAC	5 cut(s)	263, 320, 1600, 1636, 1673
TspDTI	ATGAA	5 cut(s)	656, 946, 968, 1238, 1374
TspRI	CASTG	3 cut(s)	266, 1331, 1667
Tth111I	GACNNNGTC	2 cut(s)	380, 1177
VpaK11BI	GGWCC	3 cut(s)	187, 278, 1179
XagI	CCTNNNNNAGG	1 cut(s)	1155
XapI	RAATTY	4 cut(s)	559, 916, 1225, 1310
XmiI	GTMKAC	2 cut(s)	133, 384
XmnI	GAANNNNTTC	1 cut(s)	689
XspI	CTAG	1 cut(s)	1619
ZraI	GACGTC	1 cut(s)	583
Zsp2I	ATGCAT	1 cut(s)	1561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.