MD02G1033000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014629
Physical Location & Seq
Forward (+)
2595231 .. 2596817
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1033000.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGATTCAGAAAACCGTTAGAAGCAAAGGCCTTGTTTCCCTCCCACAAAAATGCAAAACCCTAAACCAGCTCAAGCAAATCCACGCCCACATCCTTACATGCCGTCTACCCGAAAACCTGTACGCCATTGCTCCGCTCCTCTCCGCGGCAGCAACCTCCAAAGATGCTTCCTTTTTATCCTACGCGTGCTCAATTTTCAAGCACCACCATAACCACCGCAACATCTTCATGTACAACACCATGATCCGAGGATGTGTTCAGTCGCACTCGCCGGTTCCCGCCATTTCGTACTATTTAGACATGTTGAATTATGGGTTTGTTCCGAATAACTACACTTTTCCGCCGTTGATTAAAGCGTGCACGATTCTCGCACTGAATTTGAAGCTCAGTGGTCGATTAGTTCATGGCCATGCGATTCAGTTCGGGTATGGTGACGATCCTTTTGTTATTAGCTCGCTGATTGAGTTTTATGCCGTAATCTATGATATGGAAACTGCAAAGATGGTGTTTGATAAAAGTCCTAACAGGGATGTGGTAGTGTGGACGGCAATGATTGACGGGTATGGGAAGACAGGGAATGTTGAGAATGCCCGAGCTTTGTTTGACAAAATGCCGGAGAGAAACACCATATCTTGGAGTGCGATGATGGCTGCGTATTCTCGGGTGAACGACTTCAGGGAGGTGCTTTTTTTATTTAGGCAAATGCAAGAAGCAGGCACAAAGCCTAATGAGTCGGTTCTTGTTAGTGTTCTGACTGCCTCTGCTCATCTCGGTGCGGTCACACAAGGACTGTGGGTGCACTCCTATGCTAAAAGGTGCAATCTTGTATTGAACACGAGATTAGCCACTGCTCTGGTTGATATGTACTCGAAATGCGGATATGTGGAAGCAGCATTACAGGTTTTTGAGGGCATTCTTAACAAGGATGCTAAAGCATGGAACGCTATGATCGCTGGAGTTGCAATTAATGGGGATGCATTGAAATCGCTTGAACTCTTCAATAATATGGTTTTAGATAGGATACAACCCACAGAGACCACATTTGTTGCCGTTCTCTCGGCTTGCACACATGCAAAAACGGTTGACAAGGGCCTTGAATTGTTTGATCAAATGGAGACCCTTTACGGGGTTAAACCTGGGCTTGAACATTACGCTTGCATTGTTGACCTATTGGCCAGAGCAGGCATGGTGGAGGAAGCTGAGAAATTTATAGAGGACAAGATGGGAGGGCTAGGAAGAGGGGATGCCAACGTGTGGGGAGCATTACTGGCTGCATGTAGAGTTCATGGGAATGTTGAAGTTGGTGACAGAACTTGGAAGAAGCTTGCAGATATGGGAGTAGCCGACTGTGGCATTCATGTTCTTTCGTATAATATGTACAAGGAAGCTGGCTGGGAATTGGAGGCGAAGAGAGTCAGAAATATGATCTATGAAGCGGGAATGAATAAGAAACCTGGTTCCAGTGTGATAGAGGTAAATGGCGCGGCCAAAGAATTCTTTGTTGGTGATCTTTGTCATCCACAAGCAGAAGAAATTGTTAATATGCTCAACTCCTTTCGTAAATTGGTGAATTTTGAGGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

529

Amino Acids

58.69

Weight (kDa)

7.23

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 120 1e-07 PPR repeat family
PPR_2 PF13041 176 - 208 2.8e-07 PPR repeat family
PPR_1 PF12854 176 - 204 3.3e-06 PPR repeat
PPR PF01535 179 - 208 5e-08 PPR repeat
PPR_2 PF13041 208 - 244 6.4e-06 PPR repeat family
PPR_long PF17177 293 - 410 2.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 309 - 356 3.1e-06 PPR repeat family
E_motif PF20431 429 - 491 1.5e-07 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03350
malus_domestica MD02G1033000.v1.1
prunus_persica Prupe.7G242300_v2.0.a1
pyrus_communis pycom02g01480 pycom02g02710 pycom04g20500
rosa_chinensis RchiOBHm_Chr2g0088571
rosa_laevigata RLG00000015966
rosa_multiflora Rmu_co8371145.1_g000001 Rmu_sc0004733.1_g000003
rosa_roxburghii Rroxscaffold_2G00152540
rosa_rugosa Rorug01G0485200
rosa_samantha Rh2AG040500 Rh2BG039200 Rh2CG040100 Rh2DG039500
rosa_wichuraiana Rw2G003140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 633, 1254
AccBSI CCGCTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 106
AccII CGCG 3 cut(s) 146, 185, 1484
AclWI GGATC 2 cut(s) 238, 431
AcoI YGGCCR 3 cut(s) 406, 1173, 1485
AcsI RAATTY 4 cut(s) 376, 1205, 1493, 1570
AcuI CTGAAG 1 cut(s) 658
AfaI GTAC 5 cut(s) 122, 233, 290, 866, 1379
AfiI CCNNNNNNNGG 5 cut(s) 145, 633, 1125, 1126, 1254
AflIII ACRYGT 3 cut(s) 183, 300, 1251
AjnI CCWGG 2 cut(s) 1135, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 1485, 1517
AluBI AGCT 7 cut(s) 70, 385, 453, 596, 1199, 1324, 1388
AluI AGCT 7 cut(s) 70, 385, 453, 596, 1199, 1324, 1388
Alw21I GWGCWC 3 cut(s) 191, 362, 801
Alw26I GTCTC 2 cut(s) 1028, 1109
Alw44I GTGCAC 2 cut(s) 358, 797
AlwI GGATC 2 cut(s) 238, 431
Ama87I CYCGRG 2 cut(s) 591, 660
AoxI GGCC 5 cut(s) 28, 406, 1090, 1173, 1485
ApaLI GTGCAC 2 cut(s) 358, 797
ApeKI GCWGC 4 cut(s) 149, 650, 890, 1271
ApoI RAATTY 4 cut(s) 376, 1205, 1493, 1570
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 966
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1484
AspS9I GGNCC 1 cut(s) 1090
AsuHPI GGTGA 5 cut(s) 443, 676, 1316, 1517, 1579
AvaI CYCGRG 2 cut(s) 591, 660
BaeGI GKGCMC 2 cut(s) 362, 801
BalI TGGCCA 2 cut(s) 408, 1175
BarI GAAGNNNNNNTAC 2 cut(s) 879, 911
BauI CACGAG 1 cut(s) 835
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 3 cut(s) 191, 362, 801
BbvI GCAGC 4 cut(s) 161, 637, 902, 1258
BccI CCATC 3 cut(s) 496, 640, 1216
BceAI ACGGC 5 cut(s) 87, 328, 458, 561, 1034
BcgI CGANNNNNNTGC 4 cut(s) 349, 383, 966, 1000
BciT130I CCWGG 2 cut(s) 1137, 1455
BciVI GTATCC 1 cut(s) 1014
BclI TGATCA 1 cut(s) 1105
BcoDI GTCTC 2 cut(s) 1028, 1109
BfaI CTAG 1 cut(s) 1232
BfuI GTATCC 1 cut(s) 1014
BisI GCNGC 6 cut(s) 147, 150, 651, 891, 1272, 1485
BlsI GCNGC 6 cut(s) 148, 151, 652, 892, 1273, 1486
Bme1390I CCNGG 2 cut(s) 1137, 1455
BmeT110I CYCGRG 2 cut(s) 591, 660
BmgT120I GGNCC 1 cut(s) 1090
BmiI GGNNCC 2 cut(s) 276, 1459
BmrFI CCNGG 2 cut(s) 1137, 1455
BmsI GCATC 4 cut(s) 154, 916, 964, 1234
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 56
BsaI GGTCTC 2 cut(s) 1028, 1109
BsaJI CCNNGG 3 cut(s) 144, 247, 1136
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 633, 1125, 1126, 1254
Bse118I RCCGGY 1 cut(s) 271
Bse1I ACTGG 2 cut(s) 1272, 1461
Bse3DI GCAATG 2 cut(s) 126, 555
BseBI CCWGG 2 cut(s) 1137, 1455
BseDI CCNNGG 3 cut(s) 144, 247, 1136
BseGI GGATG 7 cut(s) 90, 257, 535, 931, 979, 1249, 1516
BseLI CCNNNNNNNGG 5 cut(s) 145, 633, 1125, 1126, 1254
BseMI GCAATG 2 cut(s) 126, 555
BseMII CTCAG 2 cut(s) 400, 1191
BseNI ACTGG 2 cut(s) 1272, 1461
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 2 cut(s) 362, 801
BseXI GCAGC 4 cut(s) 161, 637, 902, 1258
BseYI CCCAGC 1 cut(s) 1392
Bsh1236I CGCG 3 cut(s) 146, 185, 1484
BshFI GGCC 5 cut(s) 30, 408, 1092, 1175, 1487
BsiHKAI GWGCWC 3 cut(s) 191, 362, 801
BsiHKCI CYCGRG 2 cut(s) 591, 660
BsiSI CCGG 2 cut(s) 272, 614
BslI CCNNNNNNNGG 5 cut(s) 145, 633, 1125, 1126, 1254
BsmAI GTCTC 2 cut(s) 1028, 1109
BsmI GAATGC 3 cut(s) 592, 912, 1353
BsnI GGCC 5 cut(s) 30, 408, 1092, 1175, 1487
Bso31I GGTCTC 2 cut(s) 1028, 1109
BsoBI CYCGRG 2 cut(s) 591, 660
Bsp1286I GDGCHC 3 cut(s) 191, 362, 801
Bsp1407I TGTACA 2 cut(s) 231, 1377
Bsp143I GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
BspANI GGCC 5 cut(s) 30, 408, 1092, 1175, 1487
BspCNI CTCAG 2 cut(s) 399, 1192
BspFNI CGCG 3 cut(s) 146, 185, 1484
BspLI GGNNCC 2 cut(s) 276, 1459
BspPI GGATC 2 cut(s) 238, 431
BspTNI GGTCTC 2 cut(s) 1028, 1109
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 126, 555
BsrFI RCCGGY 1 cut(s) 271
BsrGI TGTACA 2 cut(s) 231, 1377
BsrI ACTGG 2 cut(s) 1272, 1461
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 3 cut(s) 144, 247, 1136
BssMI GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
BssSI CACGAG 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 835
Bst2UI CCWGG 2 cut(s) 1137, 1455
Bst4CI ACNGT 4 cut(s) 16, 792, 1081, 1349
Bst6I CTCTTC 3 cut(s) 1001, 1231, 1403
BstAUI TGTACA 2 cut(s) 231, 1377
BstC8I GCNNGC 9 cut(s) 187, 358, 455, 715, 1063, 1156, 1183, 1326, 1390
BstDEI CTNAG 2 cut(s) 386, 1200
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 7 cut(s) 90, 257, 535, 931, 979, 1249, 1516
BstFNI CGCG 3 cut(s) 146, 185, 1484
BstHHI GCGC 1 cut(s) 1484
BstKTI GATC 6 cut(s) 246, 439, 951, 1108, 1428, 1510
BstMAI GTCTC 2 cut(s) 1028, 1109
BstMBI GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
BstMWI GCNNNNNNNGC 5 cut(s) 647, 941, 950, 959, 1268
BstNI CCWGG 2 cut(s) 1137, 1455
BstNSI RCATGY 4 cut(s) 102, 304, 1073, 1278
BstSCI CCNGG 2 cut(s) 1135, 1453
BstSLI GKGCMC 2 cut(s) 362, 801
BstUI CGCG 3 cut(s) 146, 185, 1484
BstV1I GCAGC 4 cut(s) 161, 637, 902, 1258
BstV2I GAAGAC 1 cut(s) 575
BstXI CCANNNNNNTGG 1 cut(s) 853
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 5 cut(s) 30, 408, 1092, 1175, 1487
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 7 cut(s) 90, 257, 535, 931, 979, 1249, 1516
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 4 cut(s) 371, 394, 846, 1468
Cac8I GCNNGC 9 cut(s) 187, 358, 455, 715, 1063, 1156, 1183, 1326, 1390
CfoI GCGC 1 cut(s) 1484
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 1090
Cfr42I CCGCGG 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 1453
Csp6I GTAC 5 cut(s) 121, 232, 289, 865, 1378
CspCI CAANNNNNGTGG 4 cut(s) 773, 808, 1027, 1062
CviQI GTAC 5 cut(s) 121, 232, 289, 865, 1378
DdeI CTNAG 2 cut(s) 386, 1200
DpnI GATC 6 cut(s) 245, 438, 950, 1107, 1427, 1509
DpnII GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
EaeI YGGCCR 3 cut(s) 406, 1173, 1485
Eam1104I CTCTTC 3 cut(s) 1001, 1231, 1403
EarI CTCTTC 3 cut(s) 1001, 1231, 1403
EciI GGCGGA 1 cut(s) 330
Eco147I AGGCCT 1 cut(s) 30
Eco31I GGTCTC 2 cut(s) 1028, 1109
Eco57I CTGAAG 1 cut(s) 658
Eco88I CYCGRG 2 cut(s) 591, 660
EcoO109I RGGNCCY 1 cut(s) 1090
EcoRI GAATTC 1 cut(s) 1493
EcoRII CCWGG 2 cut(s) 1135, 1453
EcoT22I ATGCAT 1 cut(s) 979
FauI CCCGC 2 cut(s) 286, 1429
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 147, 150, 651, 891, 1272, 1485
FokI GGATG 7 cut(s) 77, 264, 542, 938, 986, 1256, 1503
Fsp4HI GCNGC 6 cut(s) 147, 150, 651, 891, 1272, 1485
FspBI CTAG 1 cut(s) 1232
GlaI GCGC 1 cut(s) 1483
GluI GCNGC 6 cut(s) 147, 150, 651, 891, 1272, 1485
GsaI CCCAGC 1 cut(s) 1396
GsuI CTGGAG 1 cut(s) 975
HaeIII GGCC 5 cut(s) 30, 408, 1092, 1175, 1487
HapII CCGG 2 cut(s) 272, 614
HhaI GCGC 1 cut(s) 1484
Hin6I GCGC 1 cut(s) 1482
HinP1I GCGC 1 cut(s) 1482
HincII GTYRAC 2 cut(s) 1084, 1165
HindII GTYRAC 2 cut(s) 1084, 1165
HindIII AAGCTT 1 cut(s) 1322
HinfI GANTC 5 cut(s) 4, 364, 415, 731, 1413
HpaII CCGG 2 cut(s) 272, 614
HphI GGTGA 5 cut(s) 443, 676, 1316, 1517, 1579
Hpy166II GTNNAC 7 cut(s) 107, 360, 543, 667, 799, 1084, 1165
Hpy188I TCNGA 5 cut(s) 9, 248, 324, 753, 1418
Hpy8I GTNNAC 7 cut(s) 107, 360, 543, 667, 799, 1084, 1165
HpyCH4III ACNGT 4 cut(s) 16, 792, 1081, 1349
HpyCH4IV ACGT 1 cut(s) 1251
HpyF10VI GCNNNNNNNGC 5 cut(s) 647, 941, 950, 959, 1268
HpyF3I CTNAG 2 cut(s) 386, 1200
HpySE526I ACGT 1 cut(s) 1251
HspAI GCGC 1 cut(s) 1482
Ksp22I TGATCA 1 cut(s) 1105
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
LmnI GCTCC 3 cut(s) 136, 141, 1259
Lsp1109I GCAGC 4 cut(s) 161, 637, 902, 1258
LweI GCATC 4 cut(s) 154, 916, 964, 1234
MabI ACCWGGT 1 cut(s) 1453
MaeI CTAG 1 cut(s) 1232
MaeII ACGT 1 cut(s) 1251
MaeIII GTNAC 3 cut(s) 431, 778, 1304
MalI GATC 6 cut(s) 245, 438, 950, 1107, 1427, 1509
MbiI CCGCTC 1 cut(s) 136
MboI GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
MboII GAAGA 7 cut(s) 217, 580, 988, 1248, 1330, 1420, 1541
MhlI GDGCHC 3 cut(s) 191, 362, 801
MlsI TGGCCA 2 cut(s) 408, 1175
MluI ACGCGT 1 cut(s) 183
MluNI TGGCCA 2 cut(s) 408, 1175
MlyI GAGTC 2 cut(s) 740, 1422
Mox20I TGGCCA 2 cut(s) 408, 1175
Mph1103I ATGCAT 1 cut(s) 979
MroXI GAANNNNTTC 1 cut(s) 671
MscI TGGCCA 2 cut(s) 408, 1175
MseI TTAA 5 cut(s) 351, 918, 966, 1131, 1539
MslI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1290
Msp20I TGGCCA 2 cut(s) 408, 1175
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 2 cut(s) 272, 614
MspR9I CCNGG 2 cut(s) 1137, 1455
Mva1269I GAATGC 3 cut(s) 592, 912, 1353
MvaI CCWGG 2 cut(s) 1137, 1455
MvnI CGCG 3 cut(s) 146, 185, 1484
MwoI GCNNNNNNNGC 5 cut(s) 647, 941, 950, 959, 1268
NdeII GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
NlaIV GGNNCC 2 cut(s) 276, 1459
NmeAIII GCCGAG 1 cut(s) 1037
NmuCI GTSAC 3 cut(s) 431, 778, 1304
NsiI ATGCAT 1 cut(s) 979
NspI RCATGY 4 cut(s) 102, 304, 1073, 1278
PceI AGGCCT 1 cut(s) 30
PciI ACATGT 1 cut(s) 300
PctI GAATGC 3 cut(s) 592, 912, 1353
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 3 cut(s) 4, 364, 415
PflMI CCANNNNNTGG 2 cut(s) 633, 1254
PkrI GCNGC 6 cut(s) 148, 151, 652, 892, 1273, 1486
PleI GAGTC 2 cut(s) 739, 1421
PpsI GAGTC 2 cut(s) 739, 1421
PscI ACATGT 1 cut(s) 300
PshBI ATTAAT 1 cut(s) 966
Psp6I CCWGG 2 cut(s) 1135, 1453
PspFI CCCAGC 1 cut(s) 1392
PspGI CCWGG 2 cut(s) 1135, 1453
PspN4I GGNNCC 2 cut(s) 276, 1459
PspPI GGNCC 1 cut(s) 1090
RsaI GTAC 5 cut(s) 122, 233, 290, 866, 1379
RsaNI GTAC 5 cut(s) 121, 232, 289, 865, 1378
RseI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1290
SacII CCGCGG 1 cut(s) 147
SaqAI TTAA 5 cut(s) 351, 918, 966, 1131, 1539
SatI GCNGC 6 cut(s) 147, 150, 651, 891, 1272, 1485
Sau3AI GATC 6 cut(s) 243, 436, 948, 1105, 1425, 1507
Sau96I GGNCC 1 cut(s) 1090
SchI GAGTC 2 cut(s) 740, 1422
ScrFI CCNGG 2 cut(s) 1137, 1455
SduI GDGCHC 3 cut(s) 191, 362, 801
SexAI ACCWGGT 1 cut(s) 1453
SfaNI GCATC 4 cut(s) 154, 916, 964, 1234
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SmiMI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1290
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 30
SspMI CTAG 1 cut(s) 1232
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 1135, 1453
TaaI ACNGT 4 cut(s) 16, 792, 1081, 1349
TaiI ACGT 1 cut(s) 1254
TaqI TCGA 2 cut(s) 394, 869
TatI WGTACW 3 cut(s) 231, 864, 1377
TauI GCSGC 2 cut(s) 149, 1487
TfiI GAWTC 3 cut(s) 4, 364, 415
Tru1I TTAA 5 cut(s) 351, 918, 966, 1131, 1539
Tru9I TTAA 5 cut(s) 351, 918, 966, 1131, 1539
TscAI CASTG 4 cut(s) 378, 394, 853, 1468
TseFI GTSAC 3 cut(s) 431, 778, 1304
TseI GCWGC 4 cut(s) 149, 650, 890, 1271
Tsp45I GTSAC 3 cut(s) 431, 778, 1304
TspDTI ATGAA 6 cut(s) 217, 392, 1274, 1346, 1446, 1457
TspRI CASTG 4 cut(s) 378, 394, 853, 1468
Van91I CCANNNNNTGG 2 cut(s) 633, 1254
VneI GTGCAC 2 cut(s) 358, 797
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 4 cut(s) 376, 1205, 1493, 1570
XceI RCATGY 4 cut(s) 102, 304, 1073, 1278
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmiI GTMKAC 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 1 cut(s) 1232
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.