Rh2BG039200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014629
Physical Location & Seq
Forward (+)
2724349 .. 2725290
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG039200.1

Sequence Viewer

Length: 942 bp
ATGGCTGCGTATTCTCGAGTGAGTGAATTTAGAGAGGTGGTCTGTTTGTTTAGGCAAATGCAAGAGGTGGGGACGAGGCCTAATGAGTCTGTTCTTGTTAGTGTTCTCACTGCCGCTGCTCATTTGGGTGCGGTGGCGCAAGGGTTGTGGGTGCACTCCTATGCTAAGAGGCAGAGGCTTGAGTCGAATAGGATATTGGCCACTGCATTGGTTGATATGTACTCCAAATGTGGCTATGTGGAAGCAGCTATGAAGGTTTTTGAGGGCATTTGTAATAAGGATGCCAGAGCGTGGAATGCTATGATTACTGGGGTTGCGATGAATGGGGATGCAAGGAAATCGATTGAGCTGTTTGATAAAATGGTTGTAGATAAGATCCAACCTACAGAAACTACATTTGTTGCTGTTCTCTCGGCTTGTACCCATGCAAAAATGGTAGACAAGGGACTTGAATTGTTTGATCAAATGAGTACCCTTTATGGGGTTGAACCCAGGCTTCAGCACTATGCTTGTGTTGTTGACCTCTTGGCCAGAGCAGGCATGTTAAAGGAAGCTGAGAAGTTTATAGAGGACAAGATGGGAGGACTAGGAAGAGGGGATGCCAACGTGTGGGGGGCGTTACTGGGTGCGTGTAGAGTTCATGGGAATGTTGAACTGGGGGACAGAATTTGGAAAAAGCTGGTTGATATGGGAGTAGCCGATTGCGGTATTCATGTTCTTTCATATAACATGTACAAGGAAGCTGGCTTGGAATTGGAGGCAAAGAGAGTCCGAGATATGATCTCAGAAGGCGGAATGAAGAAGAAACCTGGTTCTAGTGTGATAGAGATAAATGGAGTGGCTGAAGAGTTCATTGTTGGTGATCTTTGTCCTCCACAAGCACAAGAAATGCTTAAGATACTTGATTCTTTTTGTAAAATGGTGAATTTGGAGGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

313

Amino Acids

34.49

Weight (kDa)

5.94

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 94 - 141 6.8e-08 PPR repeat family
PPR_long PF17177 105 - 194 1.2e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 214 - 276 6.9e-09 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03350
malus_domestica MD02G1033000.v1.1
prunus_persica Prupe.7G242300_v2.0.a1
pyrus_communis pycom02g01480 pycom02g02710 pycom04g20500
rosa_chinensis RchiOBHm_Chr2g0088571
rosa_laevigata RLG00000015966
rosa_multiflora Rmu_co8371145.1_g000001 Rmu_sc0004733.1_g000003
rosa_roxburghii Rroxscaffold_2G00152540
rosa_rugosa Rorug01G0485200
rosa_samantha Rh2AG040500 Rh2BG039200 Rh2CG040100 Rh2DG039500
rosa_wichuraiana Rw2G003140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 291, 609
AccI GTMKAC 1 cut(s) 438
AciI CCGC 4 cut(s) 114, 131, 705, 792
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 2 cut(s) 198, 528
AcsI RAATTY 3 cut(s) 26, 666, 925
AcuI CTGAAG 2 cut(s) 482, 864
AfaI GTAC 4 cut(s) 221, 421, 472, 734
AfiI CCNNNNNNNGG 4 cut(s) 291, 480, 481, 609
AflII CTTAAG 1 cut(s) 893
AflIII ACRYGT 2 cut(s) 606, 729
AgsI TTSAA 3 cut(s) 452, 488, 653
AjnI CCWGG 2 cut(s) 491, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AluBI AGCT 5 cut(s) 248, 349, 554, 679, 743
AluI AGCT 5 cut(s) 248, 349, 554, 679, 743
Alw21I GWGCWC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 152
AlwI GGATC 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 15
AoxI GGCC 3 cut(s) 77, 198, 528
ApaLI GTGCAC 1 cut(s) 152
ApeKI GCWGC 3 cut(s) 5, 116, 245
ApoI RAATTY 3 cut(s) 26, 666, 925
AspLEI GCGC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 872, 934
AvaI CYCGRG 1 cut(s) 15
BaeGI GKGCMC 1 cut(s) 156
BalI TGGCCA 2 cut(s) 200, 530
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 103, 257
BccI CCATC 1 cut(s) 571
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BciT130I CCWGG 2 cut(s) 493, 810
BclI TGATCA 1 cut(s) 460
BfaI CTAG 2 cut(s) 587, 816
BfmI CTRYAG 1 cut(s) 384
BfrI CTTAAG 1 cut(s) 893
BisI GCNGC 4 cut(s) 6, 114, 117, 246
BlsI GCNGC 4 cut(s) 7, 115, 118, 247
Bme1390I CCNGG 2 cut(s) 493, 810
BmeT110I CYCGRG 1 cut(s) 15
BmrFI CCNGG 2 cut(s) 493, 810
BmrI ACTGGG 3 cut(s) 318, 632, 665
BmsI GCATC 3 cut(s) 271, 319, 589
BmuI ACTGGG 3 cut(s) 318, 632, 665
BpuEI CTTGAG 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 480, 481, 609
Bse1I ACTGG 3 cut(s) 313, 627, 660
BseBI CCWGG 2 cut(s) 493, 810
BseCI ATCGAT 1 cut(s) 341
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 4 cut(s) 286, 334, 604, 940
BseLI CCNNNNNNNGG 4 cut(s) 291, 480, 481, 609
BseMII CTCAG 2 cut(s) 546, 798
BseNI ACTGG 3 cut(s) 313, 627, 660
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 2 cut(s) 103, 257
BshFI GGCC 3 cut(s) 79, 200, 530
BshVI ATCGAT 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 15
BslFI GGGAC 3 cut(s) 85, 459, 674
BslI CCNNNNNNNGG 4 cut(s) 291, 480, 481, 609
BsmFI GGGAC 3 cut(s) 85, 459, 674
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 3 cut(s) 79, 200, 530
BsoBI CYCGRG 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 375, 460, 780, 862
BspACI CCGC 4 cut(s) 114, 131, 705, 792
BspANI GGCC 3 cut(s) 79, 200, 530
BspCNI CTCAG 2 cut(s) 547, 797
BspDI ATCGAT 1 cut(s) 341
BspPI GGATC 1 cut(s) 370
BspTI CTTAAG 1 cut(s) 893
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 3 cut(s) 313, 627, 660
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 4 cut(s) 375, 460, 780, 862
Bst2UI CCWGG 2 cut(s) 493, 810
Bst6I CTCTTC 2 cut(s) 586, 840
BstAFI CTTAAG 1 cut(s) 893
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 2 cut(s) 538, 745
BstDEI CTNAG 3 cut(s) 165, 555, 784
BstF5I GGATG 4 cut(s) 286, 334, 604, 940
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 4 cut(s) 378, 463, 783, 865
BstMBI GATC 4 cut(s) 375, 460, 780, 862
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 2 cut(s) 493, 810
BstNSI RCATGY 2 cut(s) 544, 733
BstSCI CCNGG 2 cut(s) 491, 808
BstSFI CTRYAG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 103, 257
BstX2I RGATCY 1 cut(s) 375
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 1 cut(s) 375
Bsu15I ATCGAT 1 cut(s) 341
BsuRI GGCC 3 cut(s) 79, 200, 530
BsuTUI ATCGAT 1 cut(s) 341
BtgZI GCGATG 1 cut(s) 332
BtsCI GGATG 4 cut(s) 286, 334, 604, 940
BtsI GCAGTG 2 cut(s) 108, 201
BtsIMutI CAGTG 2 cut(s) 108, 201
Cac8I GCNNGC 2 cut(s) 538, 745
CfoI GCGC 1 cut(s) 139
ClaI ATCGAT 1 cut(s) 341
CsiI ACCWGGT 1 cut(s) 808
Csp6I GTAC 4 cut(s) 220, 420, 471, 733
CspCI CAANNNNNGTGG 2 cut(s) 128, 163
CviAII CATG 5 cut(s) 425, 541, 641, 713, 730
CviQI GTAC 4 cut(s) 220, 420, 471, 733
DdeI CTNAG 3 cut(s) 165, 555, 784
DpnI GATC 4 cut(s) 377, 462, 782, 864
DpnII GATC 4 cut(s) 375, 460, 780, 862
EaeI YGGCCR 2 cut(s) 198, 528
Eam1104I CTCTTC 2 cut(s) 586, 840
EarI CTCTTC 2 cut(s) 586, 840
EciI GGCGGA 1 cut(s) 807
Eco147I AGGCCT 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 482, 864
Eco88I CYCGRG 1 cut(s) 15
EcoRII CCWGG 2 cut(s) 491, 808
FaeI CATG 5 cut(s) 428, 544, 644, 716, 733
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 3 cut(s) 85, 459, 674
FatI CATG 5 cut(s) 424, 540, 640, 712, 729
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 6, 114, 117, 246
FokI GGATG 3 cut(s) 293, 341, 611
Fsp4HI GCNGC 4 cut(s) 6, 114, 117, 246
FspBI CTAG 2 cut(s) 587, 816
GlaI GCGC 1 cut(s) 138
GluI GCNGC 4 cut(s) 6, 114, 117, 246
HaeIII GGCC 3 cut(s) 79, 200, 530
HhaI GCGC 1 cut(s) 139
Hin1II CATG 5 cut(s) 428, 544, 644, 716, 733
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 4 cut(s) 86, 182, 768, 905
HphI GGTGA 2 cut(s) 872, 934
Hpy166II GTNNAC 3 cut(s) 154, 439, 520
Hpy188I TCNGA 2 cut(s) 773, 787
Hpy188III TCNNGA 1 cut(s) 15
Hpy8I GTNNAC 3 cut(s) 154, 439, 520
HpyAV CCTTC 2 cut(s) 247, 782
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 5 cut(s) 61, 154, 206, 332, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 3 cut(s) 165, 555, 784
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 5 cut(s) 428, 544, 644, 716, 733
HspAI GCGC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 4 cut(s) 375, 460, 780, 862
Lsp1109I GCAGC 2 cut(s) 103, 257
LweI GCATC 3 cut(s) 271, 319, 589
MabI ACCWGGT 1 cut(s) 808
MaeI CTAG 2 cut(s) 587, 816
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 1 cut(s) 618
MalI GATC 4 cut(s) 377, 462, 782, 864
MboI GATC 4 cut(s) 375, 460, 780, 862
MboII GAAGA 4 cut(s) 603, 811, 814, 857
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 156
MlsI TGGCCA 2 cut(s) 200, 530
MluCI AATT 5 cut(s) 26, 452, 666, 752, 925
MluNI TGGCCA 2 cut(s) 200, 530
MlyI GAGTC 3 cut(s) 95, 191, 777
MmeI TCCRAC 1 cut(s) 403
Mox20I TGGCCA 2 cut(s) 200, 530
MscI TGGCCA 2 cut(s) 200, 530
MseI TTAA 2 cut(s) 545, 894
MslI CAYNNNNRTG 3 cut(s) 126, 159, 645
Msp20I TGGCCA 2 cut(s) 200, 530
MspA1I CMGCKG 1 cut(s) 116
MspCI CTTAAG 1 cut(s) 893
MspR9I CCNGG 2 cut(s) 493, 810
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 2 cut(s) 493, 810
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 4 cut(s) 375, 460, 780, 862
NlaIII CATG 5 cut(s) 428, 544, 644, 716, 733
NmeAIII GCCGAG 1 cut(s) 392
NspI RCATGY 2 cut(s) 544, 733
PaeR7I CTCGAG 1 cut(s) 15
PceI AGGCCT 1 cut(s) 79
PciI ACATGT 1 cut(s) 729
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 905
PflMI CCANNNNNTGG 2 cut(s) 291, 609
PkrI GCNGC 4 cut(s) 7, 115, 118, 247
PleI GAGTC 3 cut(s) 94, 190, 776
PpsI GAGTC 3 cut(s) 94, 190, 776
PscI ACATGT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 491, 808
PspGI CCWGG 2 cut(s) 491, 808
PsuI RGATCY 1 cut(s) 375
RsaI GTAC 4 cut(s) 221, 421, 472, 734
RsaNI GTAC 4 cut(s) 220, 420, 471, 733
RseI CAYNNNNRTG 3 cut(s) 126, 159, 645
SaqAI TTAA 2 cut(s) 545, 894
SatI GCNGC 4 cut(s) 6, 114, 117, 246
Sau3AI GATC 4 cut(s) 375, 460, 780, 862
SchI GAGTC 3 cut(s) 95, 191, 777
ScrFI CCNGG 2 cut(s) 493, 810
SduI GDGCHC 1 cut(s) 156
SexAI ACCWGGT 1 cut(s) 808
SfaNI GCATC 3 cut(s) 271, 319, 589
SfcI CTRYAG 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 15
SlaI CTCGAG 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 126, 159, 645
SmlI CTYRAG 3 cut(s) 15, 179, 893
SmoI CTYRAG 3 cut(s) 15, 179, 893
Sse9I AATT 5 cut(s) 26, 452, 666, 752, 925
SseBI AGGCCT 1 cut(s) 79
SsiI CCGC 4 cut(s) 114, 131, 705, 792
SspMI CTAG 2 cut(s) 587, 816
StuI AGGCCT 1 cut(s) 79
StyD4I CCNGG 2 cut(s) 491, 808
TaiI ACGT 1 cut(s) 609
TaqI TCGA 3 cut(s) 16, 185, 341
TasI AATT 5 cut(s) 26, 452, 666, 752, 925
TatI WGTACW 2 cut(s) 219, 732
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 1 cut(s) 905
Tru1I TTAA 2 cut(s) 545, 894
Tru9I TTAA 2 cut(s) 545, 894
TscAI CASTG 2 cut(s) 115, 208
TseI GCWGC 3 cut(s) 5, 116, 245
TspDTI ATGAA 7 cut(s) 266, 335, 629, 701, 711, 812, 841
TspRI CASTG 2 cut(s) 115, 208
Van91I CCANNNNNTGG 2 cut(s) 291, 609
Vha464I CTTAAG 1 cut(s) 893
VneI GTGCAC 1 cut(s) 152
XapI RAATTY 3 cut(s) 26, 666, 925
XceI RCATGY 2 cut(s) 544, 733
XhoI CTCGAG 1 cut(s) 15
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 2 cut(s) 587, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.