pycom04g20500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014629
Physical Location & Seq
Forward (+)
21996324 .. 21997559
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20500.1

Sequence Viewer

Length: 1236 bp
ATGATTCAGAAAACCGTTAAAAGCAACGGCCTTGTTTCCCTCCCACAAAAATGCAAAACCCTAAACCAGCTCAAGCAAATCCACGCCCACATCCTTACATGCCGTCTACCCGAAGACCTGTACGCCATTGCTCTGCTCCTCTCCGCGGCAGCAACCTCCAAAGATGCTTCCTTTTTATCCTACGCGTGCTCAATTTTCAAGCACCACCATAACCACCGCAACATCTTCATGTACAACACCATGATCAGAGGATGTGTTCAGTCGCACTCGCCGGTTCCCGCCATTTCGTACTATTTAGACATGTTGAATTATGGGTTTGTTCCGAATAACTACACTTTTTCGCCGTTGATTAAAGCATGCACGATTCTCGCACTGAATTTGAAGCTCAGTGGTCGATTAATTCATGGCCATGTGATTCAGTTCGGGTATGGTGACGACCCTTTTGTTATTAGCTCGCTGATTGAGTTTTATGCCGTAATCTATGATATGGAAACTGCAAAGATGGTGTTTGATAAAAGTCCTAACAGGGATGTGGTAGTGTGGACGGCAATGATGGACGGGTATGGGAAGACAGGGAATGTTGAGAATGCTCGAGCCTTGTTTGACGAAATGCCAGAGAGAAACACCATATCTTGGAGTGCGATGATGGCTGCGTATTCTCGGGTGTACGACTTCAGGGAGGTGCTTTCTTTATTTAGGCAAATGCAAGAAGCAGGCACAAAGCCTAATGAGTCGGTTCTTGTTAATGTTCTGACTGCCTCTGCTCATCTCGGTGCGGTCACACAAGGACTATGGGTGCACTCCTATGCTAAAGGGTGCAATCTTGAATTGAACACGAGATTGGCCGCTACTCGAAATGCGGATATGTGGAAGCAGCATTACGAGTTTTTTAGGGCATTCTTAACAAATGCTAAAGCGTGGAACGCTATGATCGCTGGAGTTGCAATTAATAGGGATGCATTGAAATCACTTGAACTTTTGAATAAAATGGTTTTAGATAGGATACAACCCACAGAGACCACATTTGTTGCCGTTCTCTCGGCTTTCACACATGCAAAAACGGTTGACCTATTGGCTAGAGCAGGCATGGTGGAGGAAGCTGAGAAATTTAGAGAGGACAAGATGGGAGGGCTAGGAAGGGGGGATGCCAACGTGTGGGGAGCATTAGTGGCTGCATGCAGAGTTCATGGGAATGTTGAAGTTGGCAACAGAATTTGGAAGAGTTCATGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

412

Amino Acids

46.06

Weight (kDa)

8.81

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 74 - 120 3.3e-09 PPR repeat family
PPR_2 PF13041 176 - 208 1.3e-07 PPR repeat family
PPR PF01535 179 - 208 3.8e-08 PPR repeat
PPR_2 PF13041 208 - 244 7.8e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03350
malus_domestica MD02G1033000.v1.1
prunus_persica Prupe.7G242300_v2.0.a1
pyrus_communis pycom02g01480 pycom02g02710 pycom04g20500
rosa_chinensis RchiOBHm_Chr2g0088571
rosa_laevigata RLG00000015966
rosa_multiflora Rmu_co8371145.1_g000001 Rmu_sc0004733.1_g000003
rosa_roxburghii Rroxscaffold_2G00152540
rosa_rugosa Rorug01G0485200
rosa_samantha Rh2AG040500 Rh2BG039200 Rh2CG040100 Rh2DG039500
rosa_wichuraiana Rw2G003140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 633, 1155
AccI GTMKAC 1 cut(s) 106
AccII CGCG 2 cut(s) 146, 185
AciI CCGC 7 cut(s) 144, 146, 217, 279, 776, 846, 860
AcoI YGGCCR 2 cut(s) 406, 843
AcsI RAATTY 3 cut(s) 376, 1106, 1212
AcuI CTGAAG 1 cut(s) 658
AfaI GTAC 4 cut(s) 122, 233, 290, 668
AfiI CCNNNNNNNGG 3 cut(s) 145, 633, 1155
AflIII ACRYGT 3 cut(s) 183, 300, 1152
AgsI TTSAA 9 cut(s) 199, 307, 382, 827, 832, 964, 974, 982, 1199
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
AluBI AGCT 4 cut(s) 70, 385, 453, 1100
AluI AGCT 4 cut(s) 70, 385, 453, 1100
Alw21I GWGCWC 2 cut(s) 191, 801
Alw26I GTCTC 1 cut(s) 1010
Alw44I GTGCAC 1 cut(s) 797
Ama87I CYCGRG 2 cut(s) 591, 660
AoxI GGCC 3 cut(s) 28, 406, 843
ApaLI GTGCAC 1 cut(s) 797
ApeKI GCWGC 4 cut(s) 149, 650, 874, 1172
ApoI RAATTY 3 cut(s) 376, 1106, 1212
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 2 cut(s) 398, 948
AsuHPI GGTGA 1 cut(s) 443
AvaI CYCGRG 2 cut(s) 591, 660
BaeGI GKGCMC 1 cut(s) 801
BalI TGGCCA 1 cut(s) 408
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
BauI CACGAG 1 cut(s) 835
BbsI GAAGAC 2 cut(s) 120, 575
Bbv12I GWGCWC 2 cut(s) 191, 801
BbvI GCAGC 4 cut(s) 161, 637, 886, 1159
BccI CCATC 4 cut(s) 496, 547, 640, 1117
BceAI ACGGC 6 cut(s) 43, 87, 328, 458, 561, 1016
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciVI GTATCC 1 cut(s) 996
BclI TGATCA 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 1010
BfaI CTAG 2 cut(s) 1077, 1133
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 6 cut(s) 147, 150, 651, 846, 875, 1173
BlsI GCNGC 6 cut(s) 148, 151, 652, 847, 876, 1174
BmeT110I CYCGRG 2 cut(s) 591, 660
BmiI GGNNCC 1 cut(s) 276
BmsI GCATC 3 cut(s) 154, 946, 1135
BpiI GAAGAC 2 cut(s) 120, 575
BpmI CTGGAG 1 cut(s) 957
BpuEI CTTGAG 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 1010
BsaJI CCNNGG 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 633, 1155
Bse118I RCCGGY 1 cut(s) 271
Bse3DI GCAATG 2 cut(s) 126, 555
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 5 cut(s) 90, 257, 535, 961, 1150
BseLI CCNNNNNNNGG 3 cut(s) 145, 633, 1155
BseMI GCAATG 2 cut(s) 126, 555
BseMII CTCAG 2 cut(s) 400, 1092
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 4 cut(s) 161, 637, 886, 1159
Bsh1236I CGCG 2 cut(s) 146, 185
BshFI GGCC 3 cut(s) 30, 408, 845
BsiHKAI GWGCWC 2 cut(s) 191, 801
BsiHKCI CYCGRG 2 cut(s) 591, 660
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 145, 633, 1155
BsmAI GTCTC 1 cut(s) 1010
BsmI GAATGC 2 cut(s) 592, 896
BsnI GGCC 3 cut(s) 30, 408, 845
Bso31I GGTCTC 1 cut(s) 1010
BsoBI CYCGRG 2 cut(s) 591, 660
Bsp1286I GDGCHC 2 cut(s) 191, 801
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 243, 930
BspACI CCGC 7 cut(s) 144, 146, 217, 279, 776, 846, 860
BspANI GGCC 3 cut(s) 30, 408, 845
BspCNI CTCAG 2 cut(s) 399, 1093
BspFNI CGCG 2 cut(s) 146, 185
BspLI GGNNCC 1 cut(s) 276
BspTNI GGTCTC 1 cut(s) 1010
BsrDI GCAATG 2 cut(s) 126, 555
BsrFI RCCGGY 1 cut(s) 271
BsrGI TGTACA 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 2 cut(s) 243, 930
BssSI CACGAG 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 835
Bst4CI ACNGT 2 cut(s) 16, 1063
Bst6I CTCTTC 1 cut(s) 1214
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 6 cut(s) 187, 358, 455, 715, 1084, 1177
BstDEI CTNAG 2 cut(s) 386, 1101
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 5 cut(s) 90, 257, 535, 961, 1150
BstFNI CGCG 2 cut(s) 146, 185
BstKTI GATC 2 cut(s) 246, 933
BstMAI GTCTC 1 cut(s) 1010
BstMBI GATC 2 cut(s) 243, 930
BstMWI GCNNNNNNNGC 5 cut(s) 647, 923, 932, 941, 1169
BstNSI RCATGY 5 cut(s) 102, 304, 360, 1055, 1179
BstSLI GKGCMC 1 cut(s) 801
BstUI CGCG 2 cut(s) 146, 185
BstV1I GCAGC 4 cut(s) 161, 637, 886, 1159
BstV2I GAAGAC 2 cut(s) 120, 575
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 3 cut(s) 30, 408, 845
BtgI CCRYGG 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 5 cut(s) 90, 257, 535, 961, 1150
BtsIMutI CAGTG 2 cut(s) 371, 394
Cac8I GCNNGC 6 cut(s) 187, 358, 455, 715, 1084, 1177
Cfr10I RCCGGY 1 cut(s) 271
Cfr42I CCGCGG 1 cut(s) 147
Csp6I GTAC 4 cut(s) 121, 232, 289, 667
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviQI GTAC 4 cut(s) 121, 232, 289, 667
DdeI CTNAG 2 cut(s) 386, 1101
DpnI GATC 2 cut(s) 245, 932
DpnII GATC 2 cut(s) 243, 930
EaeI YGGCCR 2 cut(s) 406, 843
Eam1104I CTCTTC 1 cut(s) 1214
EarI CTCTTC 1 cut(s) 1214
Eco31I GGTCTC 1 cut(s) 1010
Eco57I CTGAAG 1 cut(s) 658
Eco88I CYCGRG 2 cut(s) 591, 660
EcoT22I ATGCAT 1 cut(s) 961
FauI CCCGC 1 cut(s) 286
FbaI TGATCA 1 cut(s) 243
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 6 cut(s) 147, 150, 651, 846, 875, 1173
FokI GGATG 5 cut(s) 77, 264, 542, 968, 1157
Fsp4HI GCNGC 6 cut(s) 147, 150, 651, 846, 875, 1173
FspBI CTAG 2 cut(s) 1077, 1133
GluI GCNGC 6 cut(s) 147, 150, 651, 846, 875, 1173
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 3 cut(s) 30, 408, 845
HapII CCGG 1 cut(s) 272
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HinfI GANTC 4 cut(s) 4, 364, 415, 731
HpaII CCGG 1 cut(s) 272
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 5 cut(s) 107, 543, 667, 799, 1066
Hpy188I TCNGA 4 cut(s) 9, 248, 324, 753
Hpy188III TCNNGA 1 cut(s) 824
Hpy8I GTNNAC 5 cut(s) 107, 543, 667, 799, 1066
HpyAV CCTTC 1 cut(s) 1131
HpyCH4III ACNGT 2 cut(s) 16, 1063
HpyCH4IV ACGT 1 cut(s) 1152
HpyF10VI GCNNNNNNNGC 5 cut(s) 647, 923, 932, 941, 1169
HpyF3I CTNAG 2 cut(s) 386, 1101
HpySE526I ACGT 1 cut(s) 1152
Ksp22I TGATCA 1 cut(s) 243
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 2 cut(s) 243, 930
LmnI GCTCC 2 cut(s) 141, 1160
Lsp1109I GCAGC 4 cut(s) 161, 637, 886, 1159
LweI GCATC 3 cut(s) 154, 946, 1135
MaeI CTAG 2 cut(s) 1077, 1133
MaeII ACGT 1 cut(s) 1152
MaeIII GTNAC 2 cut(s) 431, 778
MalI GATC 2 cut(s) 245, 932
MboI GATC 2 cut(s) 243, 930
MboII GAAGA 4 cut(s) 125, 217, 580, 1231
MhlI GDGCHC 2 cut(s) 191, 801
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 8 cut(s) 192, 307, 376, 399, 827, 945, 1106, 1212
MluI ACGCGT 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 740
MnlI CCTC 9 cut(s) 50, 149, 166, 242, 673, 769, 1087, 1108, 1121
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 961
MscI TGGCCA 1 cut(s) 408
MseI TTAA 6 cut(s) 18, 351, 398, 744, 902, 948
MslI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1191
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 272
Mva1269I GAATGC 2 cut(s) 592, 896
MvnI CGCG 2 cut(s) 146, 185
MwoI GCNNNNNNNGC 5 cut(s) 647, 923, 932, 941, 1169
NdeII GATC 2 cut(s) 243, 930
NlaIV GGNNCC 1 cut(s) 276
NmeAIII GCCGAG 1 cut(s) 1019
NmuCI GTSAC 2 cut(s) 431, 778
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 5 cut(s) 102, 304, 360, 1055, 1179
PaeI GCATGC 2 cut(s) 360, 1179
PaeR7I CTCGAG 1 cut(s) 591
PciI ACATGT 1 cut(s) 300
PctI GAATGC 2 cut(s) 592, 896
PfeI GAWTC 3 cut(s) 4, 364, 415
PflMI CCANNNNNTGG 2 cut(s) 633, 1155
PkrI GCNGC 6 cut(s) 148, 151, 652, 847, 876, 1174
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
PscI ACATGT 1 cut(s) 300
PshBI ATTAAT 2 cut(s) 398, 948
PspN4I GGNNCC 1 cut(s) 276
PspXI VCTCGAGB 1 cut(s) 591
RsaI GTAC 4 cut(s) 122, 233, 290, 668
RsaNI GTAC 4 cut(s) 121, 232, 289, 667
RseI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1191
SacII CCGCGG 1 cut(s) 147
SaqAI TTAA 6 cut(s) 18, 351, 398, 744, 902, 948
SatI GCNGC 6 cut(s) 147, 150, 651, 846, 875, 1173
Sau3AI GATC 2 cut(s) 243, 930
SchI GAGTC 1 cut(s) 740
SduI GDGCHC 2 cut(s) 191, 801
SetI ASST 9 cut(s) 72, 120, 158, 387, 455, 684, 1071, 1102, 1155
SfaNI GCATC 3 cut(s) 154, 946, 1135
Sfr274I CTCGAG 1 cut(s) 591
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 6 cut(s) 49, 227, 408, 771, 804, 1191
SmlI CTYRAG 2 cut(s) 71, 591
SmoI CTYRAG 2 cut(s) 71, 591
SphI GCATGC 2 cut(s) 360, 1179
Sse9I AATT 8 cut(s) 192, 307, 376, 399, 827, 945, 1106, 1212
SsiI CCGC 7 cut(s) 144, 146, 217, 279, 776, 846, 860
SspMI CTAG 2 cut(s) 1077, 1133
TaaI ACNGT 2 cut(s) 16, 1063
TaiI ACGT 1 cut(s) 1155
TaqI TCGA 3 cut(s) 394, 592, 853
TasI AATT 8 cut(s) 192, 307, 376, 399, 827, 945, 1106, 1212
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 149, 848
TfiI GAWTC 3 cut(s) 4, 364, 415
Tru1I TTAA 6 cut(s) 18, 351, 398, 744, 902, 948
Tru9I TTAA 6 cut(s) 18, 351, 398, 744, 902, 948
TscAI CASTG 2 cut(s) 378, 394
TseFI GTSAC 2 cut(s) 431, 778
TseI GCWGC 4 cut(s) 149, 650, 874, 1172
Tsp45I GTSAC 2 cut(s) 431, 778
TspDTI ATGAA 4 cut(s) 217, 392, 1175, 1215
TspRI CASTG 2 cut(s) 378, 394
Van91I CCANNNNNTGG 2 cut(s) 633, 1155
VneI GTGCAC 1 cut(s) 797
VspI ATTAAT 2 cut(s) 398, 948
XapI RAATTY 3 cut(s) 376, 1106, 1212
XceI RCATGY 5 cut(s) 102, 304, 360, 1055, 1179
XhoI CTCGAG 1 cut(s) 591
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 2 cut(s) 1077, 1133
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.