Rw2G003140

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014629
Physical Location & Seq
Forward (+)
3006013 .. 3007593
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003140.1

Sequence Viewer

Length: 1581 bp
ATGATTCAAAAAACCGTTAAAACCAACGATCTCATTCTCCTCTGCCAAAAATGCAAAACCCTAAACCAGCTCAAGCAAATCCACGCCCACCTCCTCACTTCCACCCGACCCGAAAACCCCTACGCCATTGCGCCGCTCCTCTCCGCCGCCGCCAAAGACGCTTCCTTGTTCTCCTACGCTTGCTCCATCTTCAAACACCACCATCACCACCGCAACACTTTCATGTACAACACCATGATCAGAGCGCACGCGCAATCACACGCGCCGGTGGCTGCGGTTCTGTGCTATCTAGACTTGTTGAATTACGGATTGATTGCTAATAAGTTTACATTTCCGCCGTTGATCAAAGCTTGCTCGATTTTGGTCCATAATTCTGAGCTAACCGGTCGGTCGGTTCATGCCCATGTTGTGAAATTCGGTTTCGGAGATGACCCGTTTGTTGTTAGCGGGCTAATCGAGTTTTATTCGGTGATTCTTGAGATGGGTGCTGCCCAATTGGTGTTTGATAAAAGTCCTAGCAAAGATGTGGTGGTGTGGACGGCGATGGTTGATGGGTATGGGAAGATTGGGGATGTTGAGAGTGCACGGAAAGTGTTCGATGAAATGCCTGAGAGAAATGCTGTGTCGTGGAGTGCGATGATGGCCGCGTATTCTCGAGTGAGCGATTTTAGAGAGGTGGTTTGTTTGTTTAGGCAAATGCAAGAGGTGGGGACGAGGCCTAATGAGTCTGTTCTTGTTAGTGTTCTCACTGCCGCTGCTCATTTGGGTGCGGTGGCACAAGGGTTGTGGGTGCACTCCTATGCTAAGAGGCACGGGCTTGAGTCGAATAGGATATTGGCCACTGCATTGGTTGATATGTACTCCAAATGTGGCTATGTGGAAGCAGCTATGAAGGTTTTTGAGGGCATTTGTAATAAGGATGCCAGAGCGTGGAATGCTATGATTACTGGGGTTGCGATGAATGGGGATGCAAGGAAATCGATTGAGCTGTTTGATAAAATGGTTGTAGATAAGATCCAACCTACAGAAACCACATTTGTTGCTGTTCTCTCGGCTTGTACCCATGCAAAAATGGTAGACAAGGGGCTTGAATTGTTTGATCAAATGAGTACCCTTTATGGGGTTGAACCCATGCTTCAGCACTATGCTTGTGTTGTTGACCTCTTGGCCAGAGCAGGCATGTTAGAGGAAGCTGAGAAGTTTATAGAGGACAAGATGGGGGGACTAGGAAGAGGGGATGCCAACGTGTGGGGGGCATTACTGGGTGCGTGTAGAGTTCATGGGAATGTTGAAGTGGGGGACAGAATTTGGAAAAAGCTGGCTGATATGGGAGTAGCCGATTGCGGTATTCATGTTCTTTCATATAACATGTACAAGGAAGCTGGCTTGGAATTGGAGGCAAAGAGAGTCCGAGATATGATCTCAGAAGGCGGAATGAAGAAGAAACCTGGTTCTAGTGTGATAGAGGTAAATGGAGTGGCTGAAGAGTTCATTGTTGGTGATCTTTGTCCTCCACAAGCACAAGAAATGCTTAAGATACTTGATTCTTTTTGTAAAATGGTGAATTTGGAGGATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

526

Amino Acids

57.98

Weight (kDa)

6.45

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 174 - 206 7.8e-07 PPR repeat family
PPR_1 PF12854 175 - 202 4.7e-06 PPR repeat
PPR PF01535 177 - 206 1.4e-07 PPR repeat
PPR_2 PF13041 307 - 354 1.4e-07 PPR repeat family
PPR_long PF17177 319 - 408 1.8e-06 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 427 - 489 8.1e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03350
malus_domestica MD02G1033000.v1.1
prunus_persica Prupe.7G242300_v2.0.a1
pyrus_communis pycom02g01480 pycom02g02710 pycom04g20500
rosa_chinensis RchiOBHm_Chr2g0088571
rosa_laevigata RLG00000015966
rosa_multiflora Rmu_co8371145.1_g000001 Rmu_sc0004733.1_g000003
rosa_roxburghii Rroxscaffold_2G00152540
rosa_rugosa Rorug01G0485200
rosa_samantha Rh2AG040500 Rh2BG039200 Rh2CG040100 Rh2DG039500
rosa_wichuraiana Rw2G003140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 930, 1248
AccBSI CCGCTC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 1077
AccII CGCG 3 cut(s) 251, 263, 647
AclWI GGATC 1 cut(s) 1009
AcoI YGGCCR 3 cut(s) 642, 837, 1167
AcsI RAATTY 3 cut(s) 413, 1305, 1564
AcuI CTGAAG 2 cut(s) 1121, 1503
AfaI GTAC 5 cut(s) 227, 860, 1060, 1111, 1373
AfiI CCNNNNNNNGG 4 cut(s) 930, 1119, 1120, 1248
AflII CTTAAG 1 cut(s) 1532
AflIII ACRYGT 2 cut(s) 1245, 1368
AgeI ACCGGT 1 cut(s) 383
AgsI TTSAA 6 cut(s) 8, 193, 301, 1091, 1127, 1292
AjnI CCWGG 1 cut(s) 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 818, 850
AluBI AGCT 8 cut(s) 70, 350, 379, 887, 988, 1193, 1318, 1382
AluI AGCT 8 cut(s) 70, 350, 379, 887, 988, 1193, 1318, 1382
Alw21I GWGCWC 2 cut(s) 586, 795
Alw44I GTGCAC 2 cut(s) 582, 791
AlwI GGATC 1 cut(s) 1009
Ama87I CYCGRG 1 cut(s) 654
AoxI GGCC 4 cut(s) 642, 716, 837, 1167
ApaLI GTGCAC 2 cut(s) 582, 791
ApeKI GCWGC 4 cut(s) 272, 488, 755, 884
ApoI RAATTY 3 cut(s) 413, 1305, 1564
AsiGI ACCGGT 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 593
AspLEI GCGC 4 cut(s) 133, 247, 253, 265
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 4 cut(s) 197, 481, 1511, 1573
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 1 cut(s) 364
BaeGI GKGCMC 2 cut(s) 586, 795
BalI TGGCCA 2 cut(s) 839, 1169
Bbv12I GWGCWC 2 cut(s) 586, 795
BbvI GCAGC 4 cut(s) 259, 475, 742, 896
BccI CCATC 7 cut(s) 194, 210, 475, 538, 545, 634, 1210
BceAI ACGGC 2 cut(s) 322, 555
BcgI CGANNNNNNTGC 2 cut(s) 960, 994
BciT130I CCWGG 1 cut(s) 1449
BclI TGATCA 3 cut(s) 237, 342, 1099
BfaI CTAG 4 cut(s) 290, 516, 1226, 1455
BfmI CTRYAG 1 cut(s) 1023
BfrI CTTAAG 1 cut(s) 1532
BisI GCNGC 9 cut(s) 134, 147, 150, 273, 489, 645, 753, 756, 885
BlsI GCNGC 9 cut(s) 135, 148, 151, 274, 490, 646, 754, 757, 886
Bme1390I CCNGG 1 cut(s) 1449
Bme18I GGWCC 1 cut(s) 364
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 1449
BmrI ACTGGG 2 cut(s) 957, 1271
BmsI GCATC 3 cut(s) 910, 958, 1228
BmuI ACTGGG 2 cut(s) 957, 1271
BpuEI CTTGAG 3 cut(s) 56, 497, 839
Bsa29I ATCGAT 1 cut(s) 980
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 930, 1119, 1120, 1248
Bse118I RCCGGY 2 cut(s) 265, 383
Bse1I ACTGG 2 cut(s) 952, 1266
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 1449
BseCI ATCGAT 1 cut(s) 980
BseGI GGATG 5 cut(s) 577, 925, 973, 1243, 1579
BseLI CCNNNNNNNGG 4 cut(s) 930, 1119, 1120, 1248
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 4 cut(s) 366, 600, 1185, 1437
BseNI ACTGG 2 cut(s) 952, 1266
BseRI GAGGAG 3 cut(s) 29, 83, 128
BseSI GKGCMC 2 cut(s) 586, 795
BseXI GCAGC 4 cut(s) 259, 475, 742, 896
Bsh1236I CGCG 3 cut(s) 251, 263, 647
Bsh1285I CGRYCG 2 cut(s) 388, 392
BshFI GGCC 4 cut(s) 644, 718, 839, 1169
BshTI ACCGGT 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 980
BsiEI CGRYCG 2 cut(s) 388, 392
BsiHKAI GWGCWC 2 cut(s) 586, 795
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 2 cut(s) 266, 384
BslFI GGGAC 3 cut(s) 724, 1236, 1313
BslI CCNNNNNNNGG 4 cut(s) 930, 1119, 1120, 1248
BsmFI GGGAC 3 cut(s) 724, 1236, 1313
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 4 cut(s) 644, 718, 839, 1169
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 2 cut(s) 586, 795
Bsp1407I TGTACA 2 cut(s) 225, 1371
Bsp143I GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
BspANI GGCC 4 cut(s) 644, 718, 839, 1169
BspCNI CTCAG 4 cut(s) 367, 601, 1186, 1436
BspDI ATCGAT 1 cut(s) 980
BspFNI CGCG 3 cut(s) 251, 263, 647
BspPI GGATC 1 cut(s) 1009
BspTI CTTAAG 1 cut(s) 1532
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 2 cut(s) 265, 383
BsrGI TGTACA 2 cut(s) 225, 1371
BsrI ACTGG 2 cut(s) 952, 1266
BssAI RCCGGY 2 cut(s) 265, 383
BssMI GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
Bst2UI CCWGG 1 cut(s) 1449
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 2 cut(s) 1225, 1479
BstAFI CTTAAG 1 cut(s) 1532
BstAUI TGTACA 2 cut(s) 225, 1371
BstC8I GCNNGC 7 cut(s) 181, 249, 352, 449, 1177, 1320, 1384
BstDEI CTNAG 5 cut(s) 375, 609, 804, 1194, 1423
BstF5I GGATG 5 cut(s) 577, 925, 973, 1243, 1579
BstFNI CGCG 3 cut(s) 251, 263, 647
BstHHI GCGC 4 cut(s) 133, 247, 253, 265
BstKTI GATC 7 cut(s) 31, 240, 345, 1017, 1102, 1422, 1504
BstMBI GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
BstMCI CGRYCG 2 cut(s) 388, 392
BstMWI GCNNNNNNNGC 5 cut(s) 51, 158, 269, 641, 935
BstNI CCWGG 1 cut(s) 1449
BstNSI RCATGY 2 cut(s) 1183, 1372
BstSCI CCNGG 1 cut(s) 1447
BstSFI CTRYAG 1 cut(s) 1023
BstSLI GKGCMC 2 cut(s) 586, 795
BstUI CGCG 3 cut(s) 251, 263, 647
BstV1I GCAGC 4 cut(s) 259, 475, 742, 896
BstX2I RGATCY 1 cut(s) 1014
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 1 cut(s) 1014
Bsu15I ATCGAT 1 cut(s) 980
BsuRI GGCC 4 cut(s) 644, 718, 839, 1169
BsuTUI ATCGAT 1 cut(s) 980
BtgZI GCGATG 3 cut(s) 557, 650, 971
BtsCI GGATG 5 cut(s) 577, 925, 973, 1243, 1579
BtsI GCAGTG 2 cut(s) 747, 840
BtsIMutI CAGTG 2 cut(s) 747, 840
Cac8I GCNNGC 7 cut(s) 181, 249, 352, 449, 1177, 1320, 1384
CfoI GCGC 4 cut(s) 133, 247, 253, 265
Cfr10I RCCGGY 2 cut(s) 265, 383
Cfr13I GGNCC 1 cut(s) 364
ClaI ATCGAT 1 cut(s) 980
CseI GACGC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 1447
Csp6I GTAC 5 cut(s) 226, 859, 1059, 1110, 1372
CspAI ACCGGT 1 cut(s) 383
CspCI CAANNNNNGTGG 4 cut(s) 767, 802, 1021, 1056
CviQI GTAC 5 cut(s) 226, 859, 1059, 1110, 1372
DdeI CTNAG 5 cut(s) 375, 609, 804, 1194, 1423
DpnI GATC 7 cut(s) 30, 239, 344, 1016, 1101, 1421, 1503
DpnII GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
EaeI YGGCCR 3 cut(s) 642, 837, 1167
Eam1104I CTCTTC 2 cut(s) 1225, 1479
EarI CTCTTC 2 cut(s) 1225, 1479
EciI GGCGGA 3 cut(s) 133, 324, 1446
Eco147I AGGCCT 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 1121, 1503
Eco88I CYCGRG 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 1447
FalI AAGNNNNNCTT 2 cut(s) 1515, 1547
FaqI GGGAC 3 cut(s) 724, 1236, 1313
FauI CCCGC 1 cut(s) 440
FbaI TGATCA 3 cut(s) 237, 342, 1099
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 9 cut(s) 134, 147, 150, 273, 489, 645, 753, 756, 885
FokI GGATG 4 cut(s) 584, 932, 980, 1250
Fsp4HI GCNGC 9 cut(s) 134, 147, 150, 273, 489, 645, 753, 756, 885
FspBI CTAG 4 cut(s) 290, 516, 1226, 1455
GlaI GCGC 4 cut(s) 132, 246, 252, 264
GluI GCNGC 9 cut(s) 134, 147, 150, 273, 489, 645, 753, 756, 885
HaeIII GGCC 4 cut(s) 644, 718, 839, 1169
HapII CCGG 2 cut(s) 266, 384
HgaI GACGC 1 cut(s) 167
HhaI GCGC 4 cut(s) 133, 247, 253, 265
Hin6I GCGC 4 cut(s) 131, 245, 251, 263
HinP1I GCGC 4 cut(s) 131, 245, 251, 263
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 6 cut(s) 4, 472, 725, 821, 1407, 1544
HpaII CCGG 2 cut(s) 266, 384
HphI GGTGA 4 cut(s) 197, 481, 1511, 1573
Hpy166II GTNNAC 6 cut(s) 327, 537, 584, 793, 1078, 1159
Hpy188I TCNGA 5 cut(s) 242, 376, 425, 1412, 1426
Hpy188III TCNNGA 3 cut(s) 290, 476, 654
Hpy8I GTNNAC 6 cut(s) 327, 537, 584, 793, 1078, 1159
HpyAV CCTTC 2 cut(s) 886, 1421
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 1 cut(s) 1245
HpyCH4V TGCA 7 cut(s) 54, 584, 700, 793, 845, 971, 1067
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 158, 269, 641, 935
HpyF3I CTNAG 5 cut(s) 375, 609, 804, 1194, 1423
HpySE526I ACGT 1 cut(s) 1245
HspAI GCGC 4 cut(s) 131, 245, 251, 263
Ksp22I TGATCA 3 cut(s) 237, 342, 1099
Kzo9I GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
LmnI GCTCC 2 cut(s) 141, 188
Lsp1109I GCAGC 4 cut(s) 259, 475, 742, 896
LweI GCATC 3 cut(s) 910, 958, 1228
MabI ACCWGGT 1 cut(s) 1447
MaeI CTAG 4 cut(s) 290, 516, 1226, 1455
MaeII ACGT 1 cut(s) 1245
MalI GATC 7 cut(s) 30, 239, 344, 1016, 1101, 1421, 1503
MbiI CCGCTC 1 cut(s) 136
MboI GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
MboII GAAGA 6 cut(s) 181, 574, 1242, 1450, 1453, 1496
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 1014
MhlI GDGCHC 2 cut(s) 586, 795
MlsI TGGCCA 2 cut(s) 839, 1169
MluCI AATT 8 cut(s) 301, 370, 413, 494, 1091, 1305, 1391, 1564
MluNI TGGCCA 2 cut(s) 839, 1169
MlyI GAGTC 3 cut(s) 734, 830, 1416
MmeI TCCRAC 1 cut(s) 1042
Mox20I TGGCCA 2 cut(s) 839, 1169
MroXI GAANNNNTTC 1 cut(s) 593
MscI TGGCCA 2 cut(s) 839, 1169
MseI TTAA 2 cut(s) 18, 1533
MslI CAYNNNNRTG 5 cut(s) 221, 402, 765, 798, 1284
Msp20I TGGCCA 2 cut(s) 839, 1169
MspA1I CMGCKG 1 cut(s) 755
MspCI CTTAAG 1 cut(s) 1532
MspI CCGG 2 cut(s) 266, 384
MspR9I CCNGG 1 cut(s) 1449
MunI CAATTG 1 cut(s) 494
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 1 cut(s) 1449
MvnI CGCG 3 cut(s) 251, 263, 647
MwoI GCNNNNNNNGC 5 cut(s) 51, 158, 269, 641, 935
NdeII GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
NmeAIII GCCGAG 1 cut(s) 1031
NspI RCATGY 2 cut(s) 1183, 1372
PaeR7I CTCGAG 1 cut(s) 654
PceI AGGCCT 1 cut(s) 718
PciI ACATGT 1 cut(s) 1368
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 3 cut(s) 4, 472, 1544
PflMI CCANNNNNTGG 2 cut(s) 930, 1248
PinAI ACCGGT 1 cut(s) 383
PkrI GCNGC 9 cut(s) 135, 148, 151, 274, 490, 646, 754, 757, 886
PleI GAGTC 3 cut(s) 733, 829, 1415
PpsI GAGTC 3 cut(s) 733, 829, 1415
PscI ACATGT 1 cut(s) 1368
Psp6I CCWGG 1 cut(s) 1447
PspGI CCWGG 1 cut(s) 1447
PspPI GGNCC 1 cut(s) 364
PsuI RGATCY 1 cut(s) 1014
RsaI GTAC 5 cut(s) 227, 860, 1060, 1111, 1373
RsaNI GTAC 5 cut(s) 226, 859, 1059, 1110, 1372
RseI CAYNNNNRTG 5 cut(s) 221, 402, 765, 798, 1284
SaqAI TTAA 2 cut(s) 18, 1533
SatI GCNGC 9 cut(s) 134, 147, 150, 273, 489, 645, 753, 756, 885
Sau3AI GATC 7 cut(s) 28, 237, 342, 1014, 1099, 1419, 1501
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 3 cut(s) 734, 830, 1416
ScrFI CCNGG 1 cut(s) 1449
SduI GDGCHC 2 cut(s) 586, 795
SexAI ACCWGGT 1 cut(s) 1447
SfaNI GCATC 3 cut(s) 910, 958, 1228
SfcI CTRYAG 1 cut(s) 1023
Sfr274I CTCGAG 1 cut(s) 654
SgrAI CRCCGGYG 1 cut(s) 265
SinI GGWCC 1 cut(s) 364
SlaI CTCGAG 1 cut(s) 654
SmiMI CAYNNNNRTG 5 cut(s) 221, 402, 765, 798, 1284
SmlI CTYRAG 5 cut(s) 71, 476, 654, 818, 1532
SmoI CTYRAG 5 cut(s) 71, 476, 654, 818, 1532
Sse9I AATT 8 cut(s) 301, 370, 413, 494, 1091, 1305, 1391, 1564
SseBI AGGCCT 1 cut(s) 718
SspMI CTAG 4 cut(s) 290, 516, 1226, 1455
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 1 cut(s) 1447
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 1 cut(s) 1248
TaqI TCGA 6 cut(s) 356, 456, 597, 655, 824, 980
TaqII GACCGA 1 cut(s) 378
TasI AATT 8 cut(s) 301, 370, 413, 494, 1091, 1305, 1391, 1564
TatI WGTACW 3 cut(s) 225, 858, 1371
TauI GCSGC 5 cut(s) 136, 149, 152, 647, 755
TfiI GAWTC 3 cut(s) 4, 472, 1544
Tru1I TTAA 2 cut(s) 18, 1533
Tru9I TTAA 2 cut(s) 18, 1533
TscAI CASTG 2 cut(s) 754, 847
TseI GCWGC 4 cut(s) 272, 488, 755, 884
TspGWI ACGGA 2 cut(s) 321, 601
TspRI CASTG 2 cut(s) 754, 847
Van91I CCANNNNNTGG 2 cut(s) 930, 1248
Vha464I CTTAAG 1 cut(s) 1532
VneI GTGCAC 2 cut(s) 582, 791
VpaK11BI GGWCC 1 cut(s) 364
XapI RAATTY 3 cut(s) 413, 1305, 1564
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 2 cut(s) 1183, 1372
XhoI CTCGAG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 1077
XmnI GAANNNNTTC 1 cut(s) 593
XspI CTAG 4 cut(s) 290, 516, 1226, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.