MD02G1033800.v1.1

Signal peptidase complex catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009052
Physical Location & Seq
Forward (+)
2614047 .. 2617578
3532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1033800.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGGTGGTTCGGGGAGACCCTGGACTCCATCAAGTCCATTCAGATCAGACAAGCTCTTTCCCAAGCCGTCACTCTCGGGTTGATTGTGACGTCGGCATTGATTATATGGAAAGGGTTGATATGTATGACTGGGAGTGAATCGCCTGTGGTGGTTGTACTATCTGAAAGCATGGAGCCTGGATTTGCAAGGGGTGACATTTTGTTCTTGCATATGGGCGCGGATCCTATTCGTGCAGGAGAAATCGTGGTGTTTAATGTGGATGGACGTGATATTCCAATTGTTCATCGAGTAATTAAGGTTCATGAGGACAAAGATACTGGAGAAGTCTATGTCCTCACAAAAGGAGACAATAACTTTGATGATGACAGGAGCCTGTATGCTCGAGGCCAGCGGTGGTTGAACCGACGCCATATCATGGGGAGAGCTGTTGGGTTTTTGCCTTACGTTGGCTGGGTGACAATTATCATGACAGAAAGGCCTATAATCAAGTACGTTCTCATAGGGGCGCTGGGATTGCTGGTCATAACCTCCAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
Peptidase_S24

Protein Analysis

181

Amino Acids

19.99

Weight (kDa)

6.42

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S24 PF00717 43 - 103 1.4e-08 Peptidase S24-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 418
AccII CGCG 1 cut(s) 221
AciI CCGC 2 cut(s) 221, 394
AclWI GGATC 2 cut(s) 218, 231
AcyI GRCGYC 2 cut(s) 92, 409
AfaI GTAC 2 cut(s) 159, 494
AfiI CCNNNNNNNGG 2 cut(s) 418, 449
AgsI TTSAA 1 cut(s) 403
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 2 cut(s) 21, 178
AluBI AGCT 2 cut(s) 56, 428
AluI AGCT 2 cut(s) 56, 428
Alw26I GTCTC 2 cut(s) 11, 342
AlwI GGATC 2 cut(s) 218, 231
Ama87I CYCGRG 2 cut(s) 77, 384
AoxI GGCC 2 cut(s) 388, 479
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspLEI GCGC 2 cut(s) 221, 511
AsuHPI GGTGA 2 cut(s) 206, 469
AvaI CYCGRG 2 cut(s) 77, 384
BamHI GGATCC 1 cut(s) 223
BccI CCATC 2 cut(s) 38, 257
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 2 cut(s) 23, 180
BcoDI GTCTC 2 cut(s) 11, 342
BfoI RGCGCY 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 23, 180
BmeT110I CYCGRG 2 cut(s) 77, 384
BmgBI CACGTC 1 cut(s) 269
BmiI GGNNCC 3 cut(s) 177, 225, 374
BmrFI CCNGG 2 cut(s) 23, 180
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 342
BsaHI GRCGYC 2 cut(s) 92, 409
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 21, 534
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 449
Bse1I ACTGG 2 cut(s) 136, 325
BseBI CCWGG 2 cut(s) 23, 180
BseDI CCNNGG 2 cut(s) 21, 534
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 418, 449
BseNI ACTGG 2 cut(s) 136, 325
BseYI CCCAGC 2 cut(s) 453, 511
BsgI GTGCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 2 cut(s) 390, 481
BsiHKCI CYCGRG 2 cut(s) 77, 384
BslI CCNNNNNNNGG 2 cut(s) 418, 449
BsmAI GTCTC 2 cut(s) 11, 342
BsnI GGCC 2 cut(s) 390, 481
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 2 cut(s) 77, 384
Bsp143I GATC 2 cut(s) 45, 223
BspACI CCGC 2 cut(s) 221, 394
BspANI GGCC 2 cut(s) 390, 481
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 2 cut(s) 304, 468
BspLI GGNNCC 3 cut(s) 177, 225, 374
BspPI GGATC 2 cut(s) 218, 231
BspTNI GGTCTC 1 cut(s) 11
BsrI ACTGG 2 cut(s) 136, 325
BssECI CCNNGG 2 cut(s) 21, 534
BssMI GATC 2 cut(s) 45, 223
BssNI GRCGYC 2 cut(s) 92, 409
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 2 cut(s) 23, 180
BstACI GRCGYC 2 cut(s) 92, 409
BstC8I GCNNGC 1 cut(s) 392
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 2 cut(s) 221, 511
BstKTI GATC 2 cut(s) 48, 226
BstMAI GTCTC 2 cut(s) 11, 342
BstMBI GATC 2 cut(s) 45, 223
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 2 cut(s) 23, 180
BstSCI CCNGG 2 cut(s) 21, 178
BstUI CGCG 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuRI GGCC 2 cut(s) 390, 481
BtrI CACGTC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 392
CciI TCATGA 2 cut(s) 304, 468
CfoI GCGC 2 cut(s) 221, 511
CseI GACGC 1 cut(s) 417
Csp6I GTAC 2 cut(s) 158, 493
CviAII CATG 4 cut(s) 172, 305, 418, 469
CviJI RGCY 8 cut(s) 56, 68, 178, 375, 390, 428, 453, 481
CviKI_1 RGCY 8 cut(s) 56, 68, 178, 375, 390, 428, 453, 481
CviQI GTAC 2 cut(s) 158, 493
DpnI GATC 2 cut(s) 47, 225
DpnII GATC 2 cut(s) 45, 223
Eco130I CCWWGG 1 cut(s) 534
Eco147I AGGCCT 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 11
Eco88I CYCGRG 2 cut(s) 77, 384
EcoRII CCWGG 2 cut(s) 21, 178
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 4 cut(s) 175, 308, 421, 472
FatI CATG 4 cut(s) 171, 304, 417, 468
FauNDI CATATG 1 cut(s) 213
FokI GGATG 1 cut(s) 275
GlaI GCGC 2 cut(s) 220, 510
GsaI CCCAGC 2 cut(s) 457, 515
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 2 cut(s) 390, 481
HgaI GACGC 1 cut(s) 417
HhaI GCGC 2 cut(s) 221, 511
Hin1I GRCGYC 2 cut(s) 92, 409
Hin1II CATG 4 cut(s) 175, 308, 421, 472
Hin6I GCGC 2 cut(s) 219, 509
HinP1I GCGC 2 cut(s) 219, 509
HinfI GANTC 2 cut(s) 26, 140
HphI GGTGA 2 cut(s) 206, 469
Hpy188I TCNGA 3 cut(s) 45, 50, 166
Hpy188III TCNNGA 2 cut(s) 305, 469
Hpy99I CGWCG 2 cut(s) 97, 411
HpyCH4IV ACGT 4 cut(s) 92, 268, 447, 495
HpyCH4V TGCA 3 cut(s) 188, 211, 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpySE526I ACGT 4 cut(s) 92, 268, 447, 495
Hsp92I GRCGYC 2 cut(s) 92, 409
Hsp92II CATG 4 cut(s) 175, 308, 421, 472
HspAI GCGC 2 cut(s) 219, 509
Kzo9I GATC 2 cut(s) 45, 223
LmnI GCTCC 2 cut(s) 175, 372
MaeII ACGT 4 cut(s) 92, 268, 447, 495
MaeIII GTNAC 4 cut(s) 70, 88, 194, 457
MalI GATC 2 cut(s) 47, 225
MboI GATC 2 cut(s) 45, 223
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 223
MluCI AATT 3 cut(s) 279, 294, 462
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 4 cut(s) 301, 347, 380, 541
MseI TTAA 2 cut(s) 255, 297
MspA1I CMGCKG 1 cut(s) 394
MspR9I CCNGG 2 cut(s) 23, 180
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 2 cut(s) 23, 180
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 517
NdeI CATATG 1 cut(s) 213
NdeII GATC 2 cut(s) 45, 223
NlaIII CATG 4 cut(s) 175, 308, 421, 472
NlaIV GGNNCC 3 cut(s) 177, 225, 374
NmuCI GTSAC 4 cut(s) 70, 88, 194, 457
PaeR7I CTCGAG 1 cut(s) 384
PagI TCATGA 2 cut(s) 304, 468
PceI AGGCCT 1 cut(s) 481
PfeI GAWTC 1 cut(s) 140
PflMI CCANNNNNTGG 1 cut(s) 418
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 21, 178
PspFI CCCAGC 2 cut(s) 453, 511
PspGI CCWGG 2 cut(s) 21, 178
PspN4I GGNNCC 3 cut(s) 177, 225, 374
PspXI VCTCGAGB 1 cut(s) 384
PsrI GAACNNNNNNTAC 2 cut(s) 285, 317
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 2 cut(s) 159, 494
RsaNI GTAC 2 cut(s) 158, 493
SaqAI TTAA 2 cut(s) 255, 297
Sau3AI GATC 2 cut(s) 45, 223
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 23, 180
SetI ASST 8 cut(s) 58, 95, 271, 303, 430, 450, 498, 533
Sfr274I CTCGAG 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 384
SmoI CTYRAG 1 cut(s) 384
Sse9I AATT 3 cut(s) 279, 294, 462
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 2 cut(s) 221, 394
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 2 cut(s) 21, 178
StyI CCWWGG 1 cut(s) 534
TaiI ACGT 4 cut(s) 95, 271, 450, 498
TaqI TCGA 2 cut(s) 289, 385
TasI AATT 3 cut(s) 279, 294, 462
TatI WGTACW 1 cut(s) 157
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 2 cut(s) 255, 297
Tru9I TTAA 2 cut(s) 255, 297
TseFI GTSAC 4 cut(s) 70, 88, 194, 457
Tsp45I GTSAC 4 cut(s) 70, 88, 194, 457
TspDTI ATGAA 2 cut(s) 275, 293
Van91I CCANNNNNTGG 1 cut(s) 418
XhoI CTCGAG 1 cut(s) 384
ZraI GACGTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.