Rorug01G0485900

Signal peptidase complex catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009052
Physical Location & Seq
Forward (+)
57227423 .. 57230119
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0485900.1

Sequence Viewer

Length: 564 bp
ATGATGGGTTTGGGTTGGGTGGGAGCGATATTAGTAGGAGCAGGCTGGCTTGCTTTGGGCTATTGCTTTGGCGCGCGTTACCCTCCTTCTCGCATTTTGGTCTCAGCTAGAGCCGCCAGACTAGCCAAGCAGAAAGCTGTTGCCACTGACGGTAACGGCAAGAAGAAGAAGACCAAAGACCCCCTTGACATTGAAAAGCTCGCTGACATTCTCCAAGATTTCAAAATGGTCTTGGTGGTCAGGAATGATCTGAAGATGGGCAAAGGGAAAATTGCTGCTCAATGCAGTCATGCGACTTTGGGCCTCTACAAAAAGCTCCTCCGTCGGGCACCAAAATCTTTAAACAGGTGGGAGATGTGTGCACAACCTAAAGTTGTTCTAAAAGTAGAAACTGAAGAAGAGATGCTTGTTTTGCAAGATAGAGCAAAATCACTTAACTTACCAAACCATATTACAATTGATGCTGGGAAGACTCAGATTGCACCAAATTCAAGGACAGTGATGGCTATTCTTGGACCGATTGAAGTGGTTGATGATGTAACAGGTGGACTGAAACTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
Peptidase_S24

Protein Analysis

187

Amino Acids

20.4

Weight (kDa)

9.83

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 74 - 187 9.2e-42 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccII CGCG 2 cut(s) 74, 76
AciI CCGC 1 cut(s) 114
AcsI RAATTY 1 cut(s) 487
AcuI CTGAAG 2 cut(s) 272, 414
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 4 cut(s) 194, 223, 492, 524
AluBI AGCT 4 cut(s) 107, 137, 199, 316
AluI AGCT 4 cut(s) 107, 137, 199, 316
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 1 cut(s) 106
Alw44I GTGCAC 1 cut(s) 360
AoxI GGCC 1 cut(s) 301
ApaLI GTGCAC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 487
AspLEI GCGC 2 cut(s) 74, 76
AspS9I GGNCC 2 cut(s) 301, 515
AvaII GGWCC 1 cut(s) 515
BaeGI GKGCMC 2 cut(s) 331, 364
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 176, 476
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 250, 496
BceAI ACGGC 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 2 cut(s) 108, 122
BisI GCNGC 2 cut(s) 114, 276
BlsI GCNGC 2 cut(s) 115, 277
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 2 cut(s) 301, 515
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 2 cut(s) 393, 451
BpiI GAAGAC 2 cut(s) 176, 476
BsaI GGTCTC 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 117, 488
BsePI GCGCGC 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 308
BseSI GKGCMC 2 cut(s) 331, 364
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 464
Bsh1236I CGCG 2 cut(s) 74, 76
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 331, 364
Bsp143I GATC 1 cut(s) 247
BspACI CCGC 1 cut(s) 114
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 116, 487
BspFNI CGCG 2 cut(s) 74, 76
BspLI GGNNCC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 106
BssHII GCGCGC 1 cut(s) 72
BssMI GATC 1 cut(s) 247
Bst4CI ACNGT 2 cut(s) 152, 499
Bst6I CTCTTC 1 cut(s) 393
BstC8I GCNNGC 5 cut(s) 43, 47, 51, 74, 201
BstDEI CTNAG 2 cut(s) 103, 474
BstFNI CGCG 2 cut(s) 74, 76
BstHHI GCGC 2 cut(s) 74, 76
BstKTI GATC 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 113, 122, 412
BstSLI GKGCMC 2 cut(s) 331, 364
BstUI CGCG 2 cut(s) 74, 76
BstV1I GCAGC 1 cut(s) 262
BstV2I GAAGAC 2 cut(s) 176, 476
BsuRI GGCC 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 144, 504
Cac8I GCNNGC 5 cut(s) 43, 47, 51, 74, 201
CfoI GCGC 2 cut(s) 74, 76
Cfr13I GGNCC 2 cut(s) 301, 515
CviAII CATG 2 cut(s) 290, 559
DdeI CTNAG 2 cut(s) 103, 474
DpnI GATC 1 cut(s) 249
DpnII GATC 1 cut(s) 247
DraI TTTAAA 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 515
Eco57I CTGAAG 2 cut(s) 272, 414
FaeI CATG 2 cut(s) 293, 562
FaiI YATR 3 cut(s) 291, 450, 560
FalI AAGNNNNNCTT 4 cut(s) 168, 200, 390, 422
FatI CATG 2 cut(s) 289, 558
Fnu4HI GCNGC 2 cut(s) 114, 276
Fsp4HI GCNGC 2 cut(s) 114, 276
FspBI CTAG 2 cut(s) 108, 122
GlaI GCGC 2 cut(s) 73, 75
GluI GCNGC 2 cut(s) 114, 276
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 303
HhaI GCGC 2 cut(s) 74, 76
Hin1II CATG 2 cut(s) 293, 562
Hin6I GCGC 2 cut(s) 72, 74
HinP1I GCGC 2 cut(s) 72, 74
HinfI GANTC 1 cut(s) 472
Hpy166II GTNNAC 2 cut(s) 362, 548
Hpy188I TCNGA 2 cut(s) 252, 477
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 2 cut(s) 362, 548
Hpy99I CGWCG 1 cut(s) 327
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 2 cut(s) 152, 499
HpyCH4V TGCA 4 cut(s) 285, 362, 415, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 122, 412
HpyF3I CTNAG 2 cut(s) 103, 474
Hsp92II CATG 2 cut(s) 293, 562
HspAI GCGC 2 cut(s) 72, 74
Kzo9I GATC 1 cut(s) 247
LmnI GCTCC 3 cut(s) 23, 38, 321
LpnPI CCDG 7 cut(s) 27, 31, 130, 226, 331, 450, 528
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 393, 451
MaeI CTAG 2 cut(s) 108, 122
MaeIII GTNAC 3 cut(s) 77, 152, 538
MalI GATC 1 cut(s) 249
MboI GATC 1 cut(s) 247
MboII GAAGA 7 cut(s) 175, 178, 181, 265, 407, 410, 481
MfeI CAATTG 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 331, 364
MluCI AATT 3 cut(s) 270, 456, 487
MlyI GAGTC 1 cut(s) 466
MnlI CCTC 3 cut(s) 93, 314, 329
MseI TTAA 2 cut(s) 341, 435
MunI CAATTG 1 cut(s) 456
MvnI CGCG 2 cut(s) 74, 76
MwoI GCNNNNNNNGC 3 cut(s) 113, 122, 412
NdeII GATC 1 cut(s) 247
NlaIII CATG 2 cut(s) 293, 562
NlaIV GGNNCC 1 cut(s) 330
PauI GCGCGC 1 cut(s) 72
PkrI GCNGC 2 cut(s) 115, 277
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 301, 515
PteI GCGCGC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 341, 435
SatI GCNGC 2 cut(s) 114, 276
Sau3AI GATC 1 cut(s) 247
Sau96I GGNCC 2 cut(s) 301, 515
SchI GAGTC 1 cut(s) 466
SduI GDGCHC 2 cut(s) 331, 364
SetI ASST 7 cut(s) 109, 139, 201, 318, 350, 370, 547
SfaNI GCATC 2 cut(s) 393, 451
SinI GGWCC 1 cut(s) 515
Sse9I AATT 3 cut(s) 270, 456, 487
SsiI CCGC 1 cut(s) 114
SspMI CTAG 2 cut(s) 108, 122
TaaI ACNGT 2 cut(s) 152, 499
TaqII GACCGA 1 cut(s) 532
TasI AATT 3 cut(s) 270, 456, 487
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 2 cut(s) 341, 435
Tru9I TTAA 2 cut(s) 341, 435
TscAI CASTG 2 cut(s) 151, 504
TseI GCWGC 1 cut(s) 275
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 151, 504
VneI GTGCAC 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 1 cut(s) 487
XspI CTAG 2 cut(s) 108, 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.