Rw2G003300

Signal peptidase complex catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009052
Physical Location & Seq
Reverse (-)
3077989 .. 3080516
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003300.1

Sequence Viewer

Length: 543 bp
ATGGGTTGGTTTGGGGATACCGTGGATTCCATCAAGTCCATTCAGATCAGGCAAGCTCTCTCCCAAGCCGTCAGTCTCGGCTTGATTGTCACTTCGGCGCTGATAATATGGAAAGGGTTGATGTTGATGACTGGGAGTGAGTCACCTGTGGTGGTTGTGCTCTCTGAAAGCATGGAACCTGGCTTTGCTAGGGGTGACATTTTGTTCTTGCATATGAGCAAGGATCCTATTCGTGCAGGAGAAATTGTCGTGTTTAACGTGGATGGACGTACTATTCCAATTGTTCATCGAGTAATTAAGGTGCACGAGCGGAAAGATACTGGAGAAGATTATGTCCTCACAAAAGGAGACAATAATTCTGATGATGATAGAATCCTTTATGCTCCGGGCCAGCGTTGGTTGCAAAAACACCATATCATGGGGAGAGCTGCTGGGTTTTTGCCTTACGTTGGTTGGGTGACAATTATCATGACAGAAAAGCCTATAATTAAGTACATTATCATAGGAGCGCTGGGATTGCTGGTCATTACGTCGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
Peptidase_S24

Protein Analysis

180

Amino Acids

19.94

Weight (kDa)

6.97

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S24 PF00717 38 - 106 2.6e-09 Peptidase S24-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 418
AccBSI CCGCTC 1 cut(s) 310
AciI CCGC 1 cut(s) 310
AclWI GGATC 2 cut(s) 218, 231
AfaI GTAC 2 cut(s) 271, 494
AfeI AGCGCT 1 cut(s) 510
AfiI CCNNNNNNNGG 2 cut(s) 418, 449
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 2 cut(s) 56, 428
AluI AGCT 2 cut(s) 56, 428
Alw21I GWGCWC 2 cut(s) 162, 306
Alw26I GTCTC 2 cut(s) 80, 342
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 2 cut(s) 218, 231
Aor51HI AGCGCT 1 cut(s) 510
AoxI GGCC 1 cut(s) 388
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 428
AspLEI GCGC 2 cut(s) 100, 511
AspS9I GGNCC 1 cut(s) 388
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 3 cut(s) 135, 206, 469
BaeGI GKGCMC 1 cut(s) 306
BamHI GGATCC 1 cut(s) 223
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 2 cut(s) 162, 306
BbvI GCAGC 1 cut(s) 415
BccI CCATC 2 cut(s) 38, 257
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 180
BciVI GTATCC 1 cut(s) 10
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 80, 342
BfaI CTAG 1 cut(s) 189
BfoI RGCGCY 2 cut(s) 101, 512
BfuI GTATCC 1 cut(s) 10
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 2 cut(s) 180, 387
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 2 cut(s) 177, 225
BmrFI CCNGG 2 cut(s) 180, 387
BmrI ACTGGG 1 cut(s) 141
BmuI ACTGGG 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 342
BpuMI CCSGG 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 449
Bse1I ACTGG 2 cut(s) 136, 325
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 418, 449
BseNI ACTGG 2 cut(s) 136, 325
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 2 cut(s) 431, 511
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 2 cut(s) 162, 306
BsiSI CCGG 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 418, 449
BsmAI GTCTC 2 cut(s) 80, 342
BsnI GGCC 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 162, 306
Bsp143I GATC 2 cut(s) 45, 223
BspACI CCGC 1 cut(s) 310
BspANI GGCC 1 cut(s) 390
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 2 cut(s) 177, 225
BspPI GGATC 2 cut(s) 218, 231
BsrBI CCGCTC 1 cut(s) 310
BsrI ACTGG 2 cut(s) 136, 325
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 45, 223
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 22
BstC8I GCNNGC 2 cut(s) 54, 392
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 268
BstH2I RGCGCY 2 cut(s) 101, 512
BstHHI GCGC 2 cut(s) 100, 511
BstKTI GATC 2 cut(s) 48, 226
BstMAI GTCTC 2 cut(s) 80, 342
BstMBI GATC 2 cut(s) 45, 223
BstMWI GCNNNNNNNGC 2 cut(s) 400, 517
BstNI CCWGG 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 178, 385
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 415
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuI GTATCC 1 cut(s) 10
BsuRI GGCC 1 cut(s) 390
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 54, 392
CciI TCATGA 1 cut(s) 468
CfoI GCGC 2 cut(s) 100, 511
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 2 cut(s) 270, 493
CviAII CATG 3 cut(s) 172, 418, 469
CviJI RGCY 7 cut(s) 56, 68, 81, 183, 390, 428, 481
CviKI_1 RGCY 7 cut(s) 56, 68, 81, 183, 390, 428, 481
CviQI GTAC 2 cut(s) 270, 493
DpnI GATC 2 cut(s) 47, 225
DpnII GATC 2 cut(s) 45, 223
Eco47III AGCGCT 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 178
FaeI CATG 3 cut(s) 175, 421, 472
FatI CATG 3 cut(s) 171, 417, 468
FauNDI CATATG 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 1 cut(s) 189
GlaI GCGC 2 cut(s) 99, 510
GluI GCNGC 1 cut(s) 429
GsaI CCCAGC 2 cut(s) 435, 515
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 2 cut(s) 101, 512
HaeIII GGCC 1 cut(s) 390
HapII CCGG 1 cut(s) 386
HhaI GCGC 2 cut(s) 100, 511
Hin1II CATG 3 cut(s) 175, 421, 472
Hin6I GCGC 2 cut(s) 98, 509
HinP1I GCGC 2 cut(s) 98, 509
HinfI GANTC 3 cut(s) 26, 140, 372
HpaII CCGG 1 cut(s) 386
HphI GGTGA 3 cut(s) 135, 206, 469
Hpy166II GTNNAC 1 cut(s) 304
Hpy188I TCNGA 3 cut(s) 45, 166, 361
Hpy188III TCNNGA 1 cut(s) 469
Hpy8I GTNNAC 1 cut(s) 304
Hpy99I CGWCG 1 cut(s) 535
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 4 cut(s) 258, 268, 447, 530
HpyCH4V TGCA 4 cut(s) 211, 236, 304, 403
HpyF10VI GCNNNNNNNGC 2 cut(s) 400, 517
HpySE526I ACGT 4 cut(s) 258, 268, 447, 530
Hsp92II CATG 3 cut(s) 175, 421, 472
HspAI GCGC 2 cut(s) 98, 509
Kzo9I GATC 2 cut(s) 45, 223
LmnI GCTCC 2 cut(s) 388, 506
Lsp1109I GCAGC 1 cut(s) 415
MaeI CTAG 1 cut(s) 189
MaeII ACGT 4 cut(s) 258, 268, 447, 530
MaeIII GTNAC 4 cut(s) 88, 141, 194, 457
MalI GATC 2 cut(s) 47, 225
MbiI CCGCTC 1 cut(s) 310
MboI GATC 2 cut(s) 45, 223
MboII GAAGA 1 cut(s) 338
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 2 cut(s) 162, 306
MluCI AATT 6 cut(s) 243, 279, 294, 355, 462, 486
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 1 cut(s) 347
MseI TTAA 3 cut(s) 255, 297, 489
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 180, 387
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 400, 517
NciI CCSGG 1 cut(s) 387
NdeI CATATG 1 cut(s) 213
NdeII GATC 2 cut(s) 45, 223
NlaIII CATG 3 cut(s) 175, 421, 472
NlaIV GGNNCC 2 cut(s) 177, 225
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 4 cut(s) 88, 141, 194, 457
PagI TCATGA 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 2 cut(s) 26, 372
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 178
PspFI CCCAGC 2 cut(s) 431, 511
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 177, 225
PspPI GGNCC 1 cut(s) 388
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 2 cut(s) 271, 494
RsaNI GTAC 2 cut(s) 270, 493
SaqAI TTAA 3 cut(s) 255, 297, 489
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 2 cut(s) 45, 223
Sau96I GGNCC 1 cut(s) 388
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 180, 387
SduI GDGCHC 2 cut(s) 162, 306
SetI ASST 9 cut(s) 58, 148, 181, 261, 271, 303, 430, 450, 533
Sse9I AATT 6 cut(s) 243, 279, 294, 355, 462, 486
SsiI CCGC 1 cut(s) 310
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 178, 385
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 4 cut(s) 261, 271, 450, 533
TaqI TCGA 2 cut(s) 289, 533
TasI AATT 6 cut(s) 243, 279, 294, 355, 462, 486
TatI WGTACW 1 cut(s) 492
TfiI GAWTC 2 cut(s) 26, 372
Tru1I TTAA 3 cut(s) 255, 297, 489
Tru9I TTAA 3 cut(s) 255, 297, 489
TseFI GTSAC 4 cut(s) 88, 141, 194, 457
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 4 cut(s) 88, 141, 194, 457
TspDTI ATGAA 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 302
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.