MD15G1174600.v1.1

Signal peptidase complex catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009052
Physical Location & Seq
Forward (+)
13598676 .. 13601366
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1174600.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGGGTGGTTCGGGGAGACCCTGGACTCCATCAAGTCCATGCTCTCTCCCAAGCCCTCATTCTCGGTCCGCTTGATTGTGACGTCGGCATTGATTATTTGGAAAGGGTTGATATGTATGACTGGGAGTGAATCGCCTGTGGTGGTTGTACTATCTGAAAGCATGGAGCCTGGCTTTGCAAGGGGTGACATTTTGTTCTTGCGTATGAGCGAGGATCCCATTCGTGCAGGAGAAATCGTGGTGTTTAATGTGGATGGACGTGATATTCCAATTGTTCATCGAGTAATTAAGGTTCATGAGCAGAAAGATACTGGAGAAGTCTATGTCCTCACAAAAGGAGACAATAACGATGACGATGACAGAAGCCTGTATGCTCGGGGCCAGTGGTGGTTGCACAGACGCCATATAATGGGAAGAGCTGTTGGGTTTTTGCCTTATGCTGGCTGGGTGACAATTATCTTGACAGAAAAGCCTGTAATCAAGTACATTCTCATAGGGGCGCTGGGATTGCTGGTCATAACCTCCAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 map03060
Pfam Domains
Protein Families
Peptidase_S24

Protein Analysis

178

Amino Acids

19.81

Weight (kDa)

6.42

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S24 PF00717 40 - 121 4.2e-09 Peptidase S24-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 409
AciI CCGC 1 cut(s) 70
AclWI GGATC 2 cut(s) 209, 222
AcyI GRCGYC 2 cut(s) 83, 400
AfaI GTAC 2 cut(s) 150, 485
AfiI CCNNNNNNNGG 2 cut(s) 409, 440
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 2 cut(s) 21, 169
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 1 cut(s) 419
AluI AGCT 1 cut(s) 419
Alw26I GTCTC 2 cut(s) 11, 333
AlwI GGATC 2 cut(s) 209, 222
Ama87I CYCGRG 1 cut(s) 375
AoxI GGCC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 67, 379
AsuHPI GGTGA 2 cut(s) 197, 460
AvaI CYCGRG 1 cut(s) 375
AvaII GGWCC 1 cut(s) 67
BamHI GGATCC 1 cut(s) 214
BccI CCATC 2 cut(s) 38, 248
BciT130I CCWGG 2 cut(s) 23, 171
BcoDI GTCTC 2 cut(s) 11, 333
BfoI RGCGCY 1 cut(s) 503
Bme1390I CCNGG 2 cut(s) 23, 171
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 375
BmgBI CACGTC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 67, 379
BmiI GGNNCC 3 cut(s) 168, 216, 380
BmrFI CCNGG 2 cut(s) 23, 171
BmrI ACTGGG 1 cut(s) 132
BmuI ACTGGG 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 333
BsaHI GRCGYC 2 cut(s) 83, 400
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 21, 525
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 440
Bse1I ACTGG 3 cut(s) 127, 316, 382
BseBI CCWGG 2 cut(s) 23, 171
BseDI CCNNGG 2 cut(s) 21, 525
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 409, 440
BseNI ACTGG 3 cut(s) 127, 316, 382
BseYI CCCAGC 2 cut(s) 444, 502
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 409, 440
BsmAI GTCTC 2 cut(s) 11, 333
BsnI GGCC 1 cut(s) 381
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 375
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 1 cut(s) 70
BspANI GGCC 1 cut(s) 381
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 3 cut(s) 168, 216, 380
BspPI GGATC 2 cut(s) 209, 222
BspQI GCTCTTC 1 cut(s) 409
BspTNI GGTCTC 1 cut(s) 11
BsrI ACTGG 3 cut(s) 127, 316, 382
BssECI CCNNGG 2 cut(s) 21, 525
BssMI GATC 1 cut(s) 214
BssNI GRCGYC 2 cut(s) 83, 400
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 23, 171
Bst6I CTCTTC 1 cut(s) 409
BstACI GRCGYC 2 cut(s) 83, 400
BstC8I GCNNGC 1 cut(s) 442
BstF5I GGATG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 2 cut(s) 11, 333
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 508
BstNI CCWGG 2 cut(s) 23, 171
BstSCI CCNGG 2 cut(s) 21, 169
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 1 cut(s) 381
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 259
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 442
CciI TCATGA 1 cut(s) 295
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 67, 379
CpoI CGGWCCG 1 cut(s) 67
CseI GACGC 1 cut(s) 408
Csp6I GTAC 2 cut(s) 149, 484
CspI CGGWCCG 1 cut(s) 67
CviAII CATG 3 cut(s) 40, 163, 296
CviJI RGCY 8 cut(s) 55, 169, 174, 366, 381, 419, 444, 472
CviKI_1 RGCY 8 cut(s) 55, 169, 174, 366, 381, 419, 444, 472
CviQI GTAC 2 cut(s) 149, 484
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 21, 169
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 3 cut(s) 43, 166, 299
FatI CATG 3 cut(s) 39, 162, 295
FokI GGATG 1 cut(s) 266
GlaI GCGC 1 cut(s) 501
GsaI CCCAGC 2 cut(s) 448, 506
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 503
HaeIII GGCC 1 cut(s) 381
HgaI GACGC 1 cut(s) 408
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 2 cut(s) 83, 400
Hin1II CATG 3 cut(s) 43, 166, 299
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 2 cut(s) 26, 131
HphI GGTGA 2 cut(s) 197, 460
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 2 cut(s) 296, 460
Hpy99I CGWCG 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 83, 259
HpyCH4V TGCA 3 cut(s) 179, 227, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 508
HpySE526I ACGT 2 cut(s) 83, 259
Hsp92I GRCGYC 2 cut(s) 83, 400
Hsp92II CATG 3 cut(s) 43, 166, 299
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 1 cut(s) 214
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 166
MaeII ACGT 2 cut(s) 83, 259
MaeIII GTNAC 3 cut(s) 79, 185, 448
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 1 cut(s) 426
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 214
MluCI AATT 3 cut(s) 270, 285, 453
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 4 cut(s) 67, 205, 338, 532
MseI TTAA 2 cut(s) 246, 288
MspR9I CCNGG 2 cut(s) 23, 171
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 2 cut(s) 23, 171
MwoI GCNNNNNNNGC 1 cut(s) 508
NdeII GATC 1 cut(s) 214
NlaIII CATG 3 cut(s) 43, 166, 299
NlaIV GGNNCC 3 cut(s) 168, 216, 380
NmuCI GTSAC 3 cut(s) 79, 185, 448
PagI TCATGA 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 409
PfeI GAWTC 1 cut(s) 131
PflMI CCANNNNNTGG 1 cut(s) 409
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 21, 169
PspFI CCCAGC 2 cut(s) 444, 502
PspGI CCWGG 2 cut(s) 21, 169
PspN4I GGNNCC 3 cut(s) 168, 216, 380
PspPI GGNCC 2 cut(s) 67, 379
PsrI GAACNNNNNNTAC 2 cut(s) 276, 308
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 2 cut(s) 150, 485
RsaNI GTAC 2 cut(s) 149, 484
Rsr2I CGGWCCG 1 cut(s) 67
RsrII CGGWCCG 1 cut(s) 67
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 2 cut(s) 246, 288
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 2 cut(s) 67, 379
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 23, 171
SetI ASST 5 cut(s) 86, 262, 294, 421, 524
SinI GGWCC 1 cut(s) 67
Sse9I AATT 3 cut(s) 270, 285, 453
SsiI CCGC 1 cut(s) 70
StyD4I CCNGG 2 cut(s) 21, 169
StyI CCWWGG 1 cut(s) 525
TaiI ACGT 2 cut(s) 86, 262
TaqI TCGA 1 cut(s) 280
TaqII GACCGA 1 cut(s) 55
TasI AATT 3 cut(s) 270, 285, 453
TatI WGTACW 2 cut(s) 148, 483
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 2 cut(s) 246, 288
Tru9I TTAA 2 cut(s) 246, 288
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 3 cut(s) 79, 185, 448
Tsp45I GTSAC 3 cut(s) 79, 185, 448
TspDTI ATGAA 2 cut(s) 266, 284
TspRI CASTG 1 cut(s) 389
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 67
ZraI GACGTC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.