Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011379

Physical Location & Seq

Genomic Coordinates Forward (+)

2640563 .. 2644552

Gene Structure 3990 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1034500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGCGGTCAAGTCGGAGCTTTGGAATTTCGGTTGTGGATTTTCATAACACGTTGCGTTTGAAAATTAGAGCAAAAACTTCAGCAAAAAAAAAAAAAAAACACAGAGAAATGGGAGCCGGGAAGAACATCTGGAACGCCATGGAAGTAGCTGAGCAAGCTTCACATTGCTCCACCTCTCAGATCCCTTCTTCTTCCCTCTCCATCGACCCCTCCCTCCCTCTCTCTGACATCTCGCCTCTCATCATGTCACTGTGCAAGGATCTGTTTAGTAAATGGTCAAATTTGGAGAACTCTCGCTTCTCCGTGGAGACGATTTCGGGCGGCATCACAAATCTACTGCTGAAGGTGACTGTCAAGGAAGAGAATGGAAACACCGTGTCTGTGACAGTTAGGTTGTACGGGCCTAACACGGATTATGTTATCAATAGGGAACGCGAACTGCAGGCAATCAAATACCTCTCGGTTGCAGGATTTGGTGCAAATTTACTGGGAGTTTTTGCGAATGGGATGGTGCAATCTTTTATCAATGCACGTACGTTAGTCCCATCAGACATGAGAAATCCAAAGCTGGCTGCCAATATTGCCAAGGAACTGCGCAGATTCCATGAAGTGGAAATTCCAGGTTCCAAGGAACCTCAATTGTGGATTGACATGTTCAAGTTCTTTGAGAAAGCATCTGCTCTGGAATTTGACGACAATGAGAAGCAGAAAGTTTATAACACCATTTCATTTAGTGTAGTTCACAATGAAATGATTGAGCTCAAGGAATTGACGAGCCTTTTGAACGCTCCAGTCGTTTTTGCTCACAATGACTTGCTTTCTGGAAATATAATGGTTAATGACGAAGAAGGTAAACTATACTTCATCGATTTTGAGTACGGATCATACAATTATAGAGGCTTTGACATCGGGAATCACTTCAATGAATATGCAGGCTATGAATGCGACTACAGTTTATTGCCGAATAAGGATGAACAATACCACTTCTTGAGGCATTATATACAACCTACCAATCCACATGAGGTATCAGACAAGGATCTTGAAGCTCTGTATGTTGAGGCAAATACATATATGTTAGCTTCTCACTTGTATTGGGCTCTATGGGGAATAATCCAGGCAAAGTTTTCTCCGATCAATTTCGACTACCTCGGTTATTTCTTCCTGCGATACAATGAATACAAAACGCAGAAGGAAAGATGCTTCACCATGGCAAGATCACATCTCTCGCAATCTGAGACCGGCTTGAACATGAATGCGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009987 GO:0016020 GO:0019637 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044464 GO:0071704 GO:0071944 GO:0090407 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 ko01100 map00564 map01100

Pfam Domains

Protein Families

Choline_kinase

Protein Analysis

421

Amino Acids

48.07

Weight (kDa)

6.33

Isoelectric Point (pI)

35.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Choline_kinase	PF01633	102 - 393	5.3e-95	Choline/ethanolamine kinase
APH	PF01636	102 - 309	4.1e-12	Phosphotransferase enzyme family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011379)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26830
fragaria_vesca	FvH4_1g03540
malus_domestica	MD02G1034500.v1.1 MD15G1175300.v1.1
prunus_persica	Prupe.7G240800_v2.0.a1 Prupe.7G240800_v2.0.a1
pyrus_communis	pycom02g02820 pycom15g15670
rosa_chinensis	RchiOBHm_Chr2g0088751 RchiOBHm_Chr2g0088811 RchiOBHm_Chr2g0088821 RchiOBHm_Chr2g0088981 RchiOBHm_Chr2g0089031
rosa_laevigata	RLG00000015984
rosa_multiflora	Rmu_sc0005510.1_g000010
rosa_roxburghii	Rroxscaffold_2G00152330
rosa_rugosa	Rorug01G0486700
rosa_samantha	Rh2BG040700 Rh2CG042300
rosa_wichuraiana	Rw2G003530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	717
Acc16I	TGCGCA	2 cut(s)	596, 1257
AccB7I	CCANNNNNTGG	1 cut(s)	610
AccII	CGCG	1 cut(s)	435
AciI	CCGC	2 cut(s)	4, 321
AclWI	GGATC	4 cut(s)	175, 267, 889, 1044
AcsI	RAATTY	5 cut(s)	24, 280, 481, 615, 686
AcuI	CTGAAG	2 cut(s)	63, 362
AfaI	GTAC	3 cut(s)	398, 535, 878
AfiI	CCNNNNNNNGG	1 cut(s)	610
AflIII	ACRYGT	2 cut(s)	48, 651
AgsI	TTSAA	6 cut(s)	61, 658, 784, 922, 1043, 1246
AjnI	CCWGG	2 cut(s)	619, 1113
AluBI	AGCT	7 cut(s)	18, 149, 158, 568, 760, 1046, 1079
AluI	AGCT	7 cut(s)	18, 149, 158, 568, 760, 1046, 1079
Alw21I	GWGCWC	1 cut(s)	762
Alw26I	GTCTC	2 cut(s)	302, 1229
AlwI	GGATC	4 cut(s)	175, 267, 889, 1044
AoxI	GGCC	1 cut(s)	401
ApeKI	GCWGC	1 cut(s)	572
ApoI	RAATTY	5 cut(s)	24, 280, 481, 615, 686
ArsI	GACNNNNNNTTYG	2 cut(s)	763, 795
Asp700I	GAANNNNTTC	1 cut(s)	917
AspLEI	GCGC	2 cut(s)	597, 1258
AspS9I	GGNCC	1 cut(s)	401
AsuC2I	CCSGG	1 cut(s)	118
AsuHPI	GGTGA	2 cut(s)	358, 1195
BanII	GRGCYC	2 cut(s)	762, 1099
Bbv12I	GWGCWC	1 cut(s)	762
BbvI	GCAGC	1 cut(s)	559
BccI	CCATC	3 cut(s)	209, 502, 553
BciT130I	CCWGG	2 cut(s)	621, 1115
BcnI	CCSGG	1 cut(s)	118
BcoDI	GTCTC	2 cut(s)	302, 1229
BfmI	CTRYAG	2 cut(s)	440, 949
BisI	GCNGC	2 cut(s)	322, 573
BlpI	GCTNAGC	1 cut(s)	150
BlsI	GCNGC	2 cut(s)	323, 574
Bme1390I	CCNGG	3 cut(s)	118, 621, 1115
BmgT120I	GGNCC	1 cut(s)	401
BmiI	GGNNCC	3 cut(s)	115, 625, 633
BmrFI	CCNGG	3 cut(s)	118, 621, 1115
BmrI	ACTGGG	1 cut(s)	497
BmsI	GCATC	3 cut(s)	333, 683, 1187
BmuI	ACTGGG	1 cut(s)	497
BpmI	CTGGAG	1 cut(s)	774
Bpu1102I	GCTNAGC	1 cut(s)	150
BpuEI	CTTGAG	2 cut(s)	746, 1009
BpuMI	CCSGG	1 cut(s)	118
Bsa29I	ATCGAT	1 cut(s)	867
BsaAI	YACGTR	1 cut(s)	533
BsaI	GGTCTC	1 cut(s)	1229
BsaJI	CCNNGG	6 cut(s)	138, 303, 585, 627, 1147, 1206
Bsc4I	CCNNNNNNNGG	1 cut(s)	610
Bse118I	RCCGGY	1 cut(s)	1238
Bse1I	ACTGG	2 cut(s)	492, 791
Bse3DI	GCAATG	1 cut(s)	163
BseBI	CCWGG	2 cut(s)	621, 1115
BseCI	ATCGAT	1 cut(s)	867
BseDI	CCNNGG	6 cut(s)	138, 303, 585, 627, 1147, 1206
BseGI	GGATG	2 cut(s)	513, 976
BseLI	CCNNNNNNNGG	1 cut(s)	610
BseMI	GCAATG	1 cut(s)	163
BseMII	CTCAG	3 cut(s)	141, 191, 1224
BseNI	ACTGG	2 cut(s)	492, 791
BseXI	GCAGC	1 cut(s)	559
Bsh1236I	CGCG	1 cut(s)	435
BshFI	GGCC	1 cut(s)	403
BshVI	ATCGAT	1 cut(s)	867
BsiHKAI	GWGCWC	1 cut(s)	762
BsiSI	CCGG	2 cut(s)	117, 1239
BsiWI	CGTACG	1 cut(s)	533
BslFI	GGGAC	1 cut(s)	527
BslI	CCNNNNNNNGG	1 cut(s)	610
BsmAI	GTCTC	2 cut(s)	302, 1229
BsmBI	CGTCTC	1 cut(s)	302
BsmFI	GGGAC	1 cut(s)	527
BsmI	GAATGC	2 cut(s)	947, 1258
BsnI	GGCC	1 cut(s)	403
Bso31I	GGTCTC	1 cut(s)	1229
Bsp1286I	GDGCHC	2 cut(s)	762, 1099
Bsp143I	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
Bsp1720I	GCTNAGC	1 cut(s)	150
Bsp19I	CCATGG	2 cut(s)	138, 1206
BspACI	CCGC	2 cut(s)	4, 321
BspANI	GGCC	1 cut(s)	403
BspCNI	CTCAG	3 cut(s)	142, 190, 1225
BspDI	ATCGAT	1 cut(s)	867
BspFNI	CGCG	1 cut(s)	435
BspLI	GGNNCC	3 cut(s)	115, 625, 633
BspMAI	CTGCAG	1 cut(s)	444
BspPI	GGATC	4 cut(s)	175, 267, 889, 1044
BspTNI	GGTCTC	1 cut(s)	1229
BsrDI	GCAATG	1 cut(s)	163
BsrFI	RCCGGY	1 cut(s)	1238
BsrI	ACTGG	2 cut(s)	492, 791
BssAI	RCCGGY	1 cut(s)	1238
BssECI	CCNNGG	6 cut(s)	138, 303, 585, 627, 1147, 1206
BssMI	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
BssT1I	CCWWGG	4 cut(s)	138, 585, 627, 1206
Bst2UI	CCWGG	2 cut(s)	621, 1115
Bst4CI	ACNGT	5 cut(s)	252, 352, 376, 388, 953
Bst6I	CTCTTC	1 cut(s)	354
BstBAI	YACGTR	1 cut(s)	533
BstC8I	GCNNGC	4 cut(s)	156, 444, 570, 934
BstDEI	CTNAG	3 cut(s)	150, 177, 1233
BstDSI	CCRYGG	3 cut(s)	138, 303, 1206
BstF5I	GGATG	2 cut(s)	513, 976
BstFNI	CGCG	1 cut(s)	435
BstHHI	GCGC	2 cut(s)	597, 1258
BstKTI	GATC	6 cut(s)	183, 262, 884, 1039, 1134, 1217
BstMAI	GTCTC	2 cut(s)	302, 1229
BstMBI	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 581, 942
BstNI	CCWGG	2 cut(s)	621, 1115
BstNSI	RCATGY	1 cut(s)	655
BstSCI	CCNGG	3 cut(s)	116, 619, 1113
BstSFI	CTRYAG	2 cut(s)	440, 949
BstUI	CGCG	1 cut(s)	435
BstV1I	GCAGC	1 cut(s)	559
BstX2I	RGATCY	3 cut(s)	180, 259, 1036
BstYI	RGATCY	3 cut(s)	180, 259, 1036
Bsu15I	ATCGAT	1 cut(s)	867
BsuRI	GGCC	1 cut(s)	403
BsuTUI	ATCGAT	1 cut(s)	867
BtgI	CCRYGG	3 cut(s)	138, 303, 1206
BtsCI	GGATG	2 cut(s)	513, 976
BtsIMutI	CAGTG	1 cut(s)	248
Cac8I	GCNNGC	4 cut(s)	156, 444, 570, 934
CfoI	GCGC	2 cut(s)	597, 1258
Cfr10I	RCCGGY	1 cut(s)	1238
Cfr13I	GGNCC	1 cut(s)	401
ClaI	ATCGAT	1 cut(s)	867
Csp6I	GTAC	3 cut(s)	397, 534, 877
CviAII	CATG	8 cut(s)	139, 244, 553, 605, 652, 1019, 1207, 1249
CviQI	GTAC	3 cut(s)	397, 534, 877
DdeI	CTNAG	3 cut(s)	150, 177, 1233
DpnI	GATC	6 cut(s)	182, 261, 883, 1038, 1133, 1216
DpnII	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
Eam1104I	CTCTTC	1 cut(s)	354
EarI	CTCTTC	1 cut(s)	354
Ecl136II	GAGCTC	1 cut(s)	760
Eco130I	CCWWGG	4 cut(s)	138, 585, 627, 1206
Eco24I	GRGCYC	2 cut(s)	762, 1099
Eco31I	GGTCTC	1 cut(s)	1229
Eco53kI	GAGCTC	1 cut(s)	760
Eco57I	CTGAAG	2 cut(s)	63, 362
EcoICRI	GAGCTC	1 cut(s)	760
EcoRII	CCWGG	2 cut(s)	619, 1113
EcoT14I	CCWWGG	4 cut(s)	138, 585, 627, 1206
EcoT38I	GRGCYC	2 cut(s)	762, 1099
ErhI	CCWWGG	4 cut(s)	138, 585, 627, 1206
Esp3I	CGTCTC	1 cut(s)	302
FaeI	CATG	8 cut(s)	142, 247, 556, 608, 655, 1022, 1210, 1252
FaqI	GGGAC	1 cut(s)	527
FatI	CATG	8 cut(s)	138, 243, 552, 604, 651, 1018, 1206, 1248
Fnu4HI	GCNGC	2 cut(s)	322, 573
FokI	GGATG	2 cut(s)	520, 983
FriOI	GRGCYC	2 cut(s)	762, 1099
Fsp4HI	GCNGC	2 cut(s)	322, 573
FspI	TGCGCA	2 cut(s)	596, 1257
GlaI	GCGC	2 cut(s)	596, 1257
GluI	GCNGC	2 cut(s)	322, 573
GsuI	CTGGAG	1 cut(s)	774
HaeIII	GGCC	1 cut(s)	403
HapII	CCGG	2 cut(s)	117, 1239
HhaI	GCGC	2 cut(s)	597, 1258
Hin1II	CATG	8 cut(s)	142, 247, 556, 608, 655, 1022, 1210, 1252
Hin6I	GCGC	2 cut(s)	595, 1256
HinP1I	GCGC	2 cut(s)	595, 1256
HindIII	AAGCTT	1 cut(s)	156
HinfI	GANTC	2 cut(s)	600, 913
HpaII	CCGG	2 cut(s)	117, 1239
HphI	GGTGA	2 cut(s)	358, 1195
Hpy166II	GTNNAC	2 cut(s)	742, 854
Hpy188I	TCNGA	7 cut(s)	15, 180, 226, 550, 1030, 1131, 1234
Hpy188III	TCNNGA	6 cut(s)	130, 683, 822, 910, 988, 1040
Hpy8I	GTNNAC	2 cut(s)	742, 854
HpyAV	CCTTC	4 cut(s)	195, 337, 842, 1183
HpyCH4III	ACNGT	5 cut(s)	252, 352, 376, 388, 953
HpyCH4IV	ACGT	3 cut(s)	50, 532, 536
HpyCH4V	TGCA	7 cut(s)	255, 442, 467, 479, 514, 530, 932
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 581, 942
HpyF3I	CTNAG	3 cut(s)	150, 177, 1233
HpySE526I	ACGT	3 cut(s)	50, 532, 536
Hsp92II	CATG	8 cut(s)	142, 247, 556, 608, 655, 1022, 1210, 1252
HspAI	GCGC	2 cut(s)	595, 1256
Kzo9I	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
LmnI	GCTCC	4 cut(s)	15, 113, 173, 793
Lsp1109I	GCAGC	1 cut(s)	559
LweI	GCATC	3 cut(s)	333, 683, 1187
MaeII	ACGT	3 cut(s)	50, 532, 536
MaeIII	GTNAC	3 cut(s)	246, 346, 382
MalI	GATC	6 cut(s)	182, 261, 883, 1038, 1133, 1216
MboI	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
MboII	GAAGA	6 cut(s)	133, 180, 183, 371, 857, 1150
MfeI	CAATTG	1 cut(s)	638
MflI	RGATCY	3 cut(s)	180, 259, 1036
MhlI	GDGCHC	2 cut(s)	762, 1099
MroXI	GAANNNNTTC	1 cut(s)	917
MseI	TTAA	1 cut(s)	837
MslI	CAYNNNNRTG	1 cut(s)	921
MspI	CCGG	2 cut(s)	117, 1239
MspR9I	CCNGG	3 cut(s)	118, 621, 1115
MunI	CAATTG	1 cut(s)	638
Mva1269I	GAATGC	2 cut(s)	947, 1258
MvaI	CCWGG	2 cut(s)	621, 1115
MvnI	CGCG	1 cut(s)	435
MwoI	GCNNNNNNNGC	3 cut(s)	155, 581, 942
NciI	CCSGG	1 cut(s)	118
NcoI	CCATGG	2 cut(s)	138, 1206
NdeII	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
NlaIII	CATG	8 cut(s)	142, 247, 556, 608, 655, 1022, 1210, 1252
NlaIV	GGNNCC	3 cut(s)	115, 625, 633
NmuCI	GTSAC	3 cut(s)	246, 346, 382
NsbI	TGCGCA	2 cut(s)	596, 1257
NspI	RCATGY	1 cut(s)	655
PciI	ACATGT	1 cut(s)	651
PcsI	WCGNNNNNNNCGW	1 cut(s)	792
PctI	GAATGC	2 cut(s)	947, 1258
PdmI	GAANNNNTTC	1 cut(s)	917
PfeI	GAWTC	2 cut(s)	600, 913
Pfl23II	CGTACG	1 cut(s)	533
PflMI	CCANNNNNTGG	1 cut(s)	610
PkrI	GCNGC	2 cut(s)	323, 574
Ppu21I	YACGTR	1 cut(s)	533
PscI	ACATGT	1 cut(s)	651
PsiI	TTATAA	1 cut(s)	717
Psp124BI	GAGCTC	1 cut(s)	762
Psp6I	CCWGG	2 cut(s)	619, 1113
PspGI	CCWGG	2 cut(s)	619, 1113
PspLI	CGTACG	1 cut(s)	533
PspN4I	GGNNCC	3 cut(s)	115, 625, 633
PspPI	GGNCC	1 cut(s)	401
PstI	CTGCAG	1 cut(s)	444
PsuI	RGATCY	3 cut(s)	180, 259, 1036
RsaI	GTAC	3 cut(s)	398, 535, 878
RsaNI	GTAC	3 cut(s)	397, 534, 877
RseI	CAYNNNNRTG	1 cut(s)	921
SacI	GAGCTC	1 cut(s)	762
SaqAI	TTAA	1 cut(s)	837
SatI	GCNGC	2 cut(s)	322, 573
Sau3AI	GATC	6 cut(s)	180, 259, 881, 1036, 1131, 1214
Sau96I	GGNCC	1 cut(s)	401
ScrFI	CCNGG	3 cut(s)	118, 621, 1115
SduI	GDGCHC	2 cut(s)	762, 1099
SfaNI	GCATC	3 cut(s)	333, 683, 1187
SfcI	CTRYAG	2 cut(s)	440, 949
SmiMI	CAYNNNNRTG	1 cut(s)	921
SmlI	CTYRAG	2 cut(s)	761, 988
SmoI	CTYRAG	2 cut(s)	761, 988
SsiI	CCGC	2 cut(s)	4, 321
SspI	AATATT	1 cut(s)	580
SstI	GAGCTC	1 cut(s)	762
StyD4I	CCNGG	3 cut(s)	116, 619, 1113
StyI	CCWWGG	4 cut(s)	138, 585, 627, 1206
TaaI	ACNGT	5 cut(s)	252, 352, 376, 388, 953
TaiI	ACGT	3 cut(s)	53, 535, 539
TaqI	TCGA	3 cut(s)	204, 867, 1140
TauI	GCSGC	1 cut(s)	324
TfiI	GAWTC	2 cut(s)	600, 913
Tru1I	TTAA	1 cut(s)	837
Tru9I	TTAA	1 cut(s)	837
TscAI	CASTG	1 cut(s)	255
TseFI	GTSAC	3 cut(s)	246, 346, 382
TseI	GCWGC	1 cut(s)	572
Tsp45I	GTSAC	3 cut(s)	246, 346, 382
TspDTI	ATGAA	9 cut(s)	32, 621, 717, 762, 853, 939, 954, 987, 1188
TspGWI	ACGGA	3 cut(s)	292, 425, 894
TspRI	CASTG	1 cut(s)	255
Van91I	CCANNNNNTGG	1 cut(s)	610
XapI	RAATTY	5 cut(s)	24, 280, 481, 615, 686
XceI	RCATGY	1 cut(s)	655
XmnI	GAANNNNTTC	1 cut(s)	917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.