Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011379

Physical Location & Seq

Genomic Coordinates Forward (+)

13621140 .. 13624958

Gene Structure 3819 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1175300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGGGAGCGGTGAAGAACATCTGGAACGCCATGGAAGTAGCTGCGCAAGCTTCAGATTGCTCCACCTCTCAGATCCATTCTTCTTCCCTCTCCGTCGACCCCTCCCTCCCTCTCCCTCCCGTCTCGCCTCTCATCATAGAATTGTGCAAGGATCTGTTTAAGAAATGGTCAAATTTGGAGAATTCTCGCTTCTCCGTGGAGACAGTTTCCGGCGGCATCACAAATCAATTGCTGAAGGTGACTGTGAAGGAAGAGAATGGAAATACCGTGTCTGTGACAGTGCGGTTGTACGGGCCTAACACCGATTATGTTATCAATAGGGAACGCGAACTGCAGGCAATCAAATACCTCTCCGTTGCAGGATTTGGTGCAAATTTGCTTGGAGTTTTTGCCAATGGGATGGTGCAATCTTTTATCAATGCACGTACACTAGTCCCATCAGACATGAGAAATCCAAAGCTGGCTGCCGATATTGCCAAGGAACTGCGCAGATTCCATCAAGTGGAAATTCCAGGTTCCAAGGAACCTCAGTTGTGGATTGACATGTTCAAGTTCTTTGAGAAAGCATCTGCTCTTGAATTTGATGACAATGAAAAGCAGAAAGTTTATAAGACCATTTCATTTAGTGAAGTTCACAATGAAATGATTGCGCTCAAGGAATTGACAGACCGTTTTAATGCTCCAGTCGTTTTTGCTCACAATGACTTGCTTTCTGGAAATATAATGGTTAATGACGAAGAAGATAAACTCTACTTCATCGATTTTGAGTACGGATCATACAATTATAGAGGCTTTGACATTGGGAATCACTTCAATGAATATGCAGGCTATGAATGCGACTATAGTTTATACCCGAATAAGGATGAACAATACCACTTCTTGAGGCATTATATACAACCTGAAAATCAACAAGAGGTATCAGACAAGGAACTTGAAGCTCTCTATGTTGAGGCAAATACATATATGTTAGCTTCACACTTATATTGGGCTTTATGGGGAATAATCCAGGCAAAGTTTTCTCCGATCAATTTCGACTATCTTGGTTATTTCTTCCTGCGGTACAACGAATACAAAAAGCAGAAGGAAAGATGCTTCTCCATGGCAAGATCACATCTCGCGCAATCTGAGACCGGGTTAAACATGAAGGCGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009987 GO:0016020 GO:0019637 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044464 GO:0071704 GO:0071944 GO:0090407 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 ko01100 map00564 map01100

Pfam Domains

Protein Families

Choline_kinase

Protein Analysis

385

Amino Acids

44.15

Weight (kDa)

5.32

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Choline_kinase	PF01633	66 - 357	7.6e-97	Choline/ethanolamine kinase
APH	PF01636	66 - 273	9.2e-13	Phosphotransferase enzyme family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011379)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26830
fragaria_vesca	FvH4_1g03540
malus_domestica	MD02G1034500.v1.1 MD15G1175300.v1.1
prunus_persica	Prupe.7G240800_v2.0.a1 Prupe.7G240800_v2.0.a1
pyrus_communis	pycom02g02820 pycom15g15670
rosa_chinensis	RchiOBHm_Chr2g0088751 RchiOBHm_Chr2g0088811 RchiOBHm_Chr2g0088821 RchiOBHm_Chr2g0088981 RchiOBHm_Chr2g0089031
rosa_laevigata	RLG00000015984
rosa_multiflora	Rmu_sc0005510.1_g000010
rosa_roxburghii	Rroxscaffold_2G00152330
rosa_rugosa	Rorug01G0486700
rosa_samantha	Rh2BG040700 Rh2CG042300
rosa_wichuraiana	Rw2G003530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	609
Acc16I	TGCGCA	2 cut(s)	45, 488
AccB7I	CCANNNNNTGG	1 cut(s)	502
AccBSI	CCGCTC	1 cut(s)	8
AccI	GTMKAC	1 cut(s)	96
AccII	CGCG	2 cut(s)	327, 1118
AciI	CCGC	4 cut(s)	8, 213, 283, 1057
AclWI	GGATC	3 cut(s)	67, 159, 781
AcsI	RAATTY	5 cut(s)	172, 181, 373, 507, 578
AcuI	CTGAAG	2 cut(s)	36, 254
AfaI	GTAC	4 cut(s)	290, 427, 770, 1061
AfiI	CCNNNNNNNGG	2 cut(s)	502, 859
AflIII	ACRYGT	1 cut(s)	543
AgsI	TTSAA	4 cut(s)	550, 578, 814, 935
AhlI	ACTAGT	1 cut(s)	430
AjnI	CCWGG	2 cut(s)	511, 1005
AluBI	AGCT	5 cut(s)	41, 50, 460, 938, 971
AluI	AGCT	5 cut(s)	41, 50, 460, 938, 971
Alw26I	GTCTC	3 cut(s)	127, 194, 1121
AlwI	GGATC	3 cut(s)	67, 159, 781
AoxI	GGCC	1 cut(s)	293
ApeKI	GCWGC	2 cut(s)	41, 464
ApoI	RAATTY	5 cut(s)	172, 181, 373, 507, 578
Asp700I	GAANNNNTTC	1 cut(s)	809
AspLEI	GCGC	5 cut(s)	46, 489, 652, 1120, 1150
AspS9I	GGNCC	1 cut(s)	293
AsuC2I	CCSGG	1 cut(s)	1132
AsuHPI	GGTGA	2 cut(s)	22, 250
BbvI	GCAGC	2 cut(s)	28, 451
BccI	CCATC	3 cut(s)	394, 445, 504
BciT130I	CCWGG	2 cut(s)	513, 1007
BcnI	CCSGG	1 cut(s)	1132
BcoDI	GTCTC	3 cut(s)	127, 194, 1121
BcuI	ACTAGT	1 cut(s)	430
BfaI	CTAG	1 cut(s)	431
BfmI	CTRYAG	2 cut(s)	332, 841
BisI	GCNGC	3 cut(s)	42, 214, 465
BlsI	GCNGC	3 cut(s)	43, 215, 466
Bme1390I	CCNGG	3 cut(s)	513, 1007, 1132
BmgT120I	GGNCC	1 cut(s)	293
BmiI	GGNNCC	2 cut(s)	517, 525
BmrFI	CCNGG	3 cut(s)	513, 1007, 1132
BmsI	GCATC	3 cut(s)	225, 575, 1079
BpmI	CTGGAG	1 cut(s)	666
BpuEI	CTTGAG	2 cut(s)	638, 901
BpuMI	CCSGG	1 cut(s)	1132
Bsa29I	ATCGAT	1 cut(s)	759
BsaAI	YACGTR	1 cut(s)	425
BsaI	GGTCTC	1 cut(s)	1121
BsaJI	CCNNGG	5 cut(s)	30, 195, 477, 519, 1098
Bsc4I	CCNNNNNNNGG	2 cut(s)	502, 859
Bse1I	ACTGG	1 cut(s)	683
BseBI	CCWGG	2 cut(s)	513, 1007
BseCI	ATCGAT	1 cut(s)	759
BseDI	CCNNGG	5 cut(s)	30, 195, 477, 519, 1098
BseGI	GGATG	2 cut(s)	405, 868
BseLI	CCNNNNNNNGG	2 cut(s)	502, 859
BseMII	CTCAG	3 cut(s)	83, 542, 1116
BseNI	ACTGG	1 cut(s)	683
BseXI	GCAGC	2 cut(s)	28, 451
Bsh1236I	CGCG	2 cut(s)	327, 1118
BshFI	GGCC	1 cut(s)	295
BshVI	ATCGAT	1 cut(s)	759
BsiSI	CCGG	2 cut(s)	210, 1131
BslFI	GGGAC	1 cut(s)	419
BslI	CCNNNNNNNGG	2 cut(s)	502, 859
BsmAI	GTCTC	3 cut(s)	127, 194, 1121
BsmBI	CGTCTC	1 cut(s)	127
BsmFI	GGGAC	1 cut(s)	419
BsmI	GAATGC	1 cut(s)	839
BsnI	GGCC	1 cut(s)	295
Bso31I	GGTCTC	1 cut(s)	1121
Bsp143I	GATC	5 cut(s)	72, 151, 773, 1023, 1106
Bsp19I	CCATGG	2 cut(s)	30, 1098
BspACI	CCGC	4 cut(s)	8, 213, 283, 1057
BspANI	GGCC	1 cut(s)	295
BspCNI	CTCAG	3 cut(s)	82, 541, 1117
BspDI	ATCGAT	1 cut(s)	759
BspFNI	CGCG	2 cut(s)	327, 1118
BspLI	GGNNCC	2 cut(s)	517, 525
BspMAI	CTGCAG	1 cut(s)	336
BspPI	GGATC	3 cut(s)	67, 159, 781
BspTNI	GGTCTC	1 cut(s)	1121
BsrBI	CCGCTC	1 cut(s)	8
BsrI	ACTGG	1 cut(s)	683
BssECI	CCNNGG	5 cut(s)	30, 195, 477, 519, 1098
BssMI	GATC	5 cut(s)	72, 151, 773, 1023, 1106
BssT1I	CCWWGG	4 cut(s)	30, 477, 519, 1098
Bst2UI	CCWGG	2 cut(s)	513, 1007
Bst4CI	ACNGT	5 cut(s)	205, 244, 268, 280, 671
Bst6I	CTCTTC	1 cut(s)	246
BstBAI	YACGTR	1 cut(s)	425
BstC8I	GCNNGC	4 cut(s)	48, 336, 462, 826
BstDEI	CTNAG	3 cut(s)	69, 528, 1125
BstDSI	CCRYGG	3 cut(s)	30, 195, 1098
BstF5I	GGATG	2 cut(s)	405, 868
BstFNI	CGCG	2 cut(s)	327, 1118
BstHHI	GCGC	5 cut(s)	46, 489, 652, 1120, 1150
BstKTI	GATC	5 cut(s)	75, 154, 776, 1026, 1109
BstMAI	GTCTC	3 cut(s)	127, 194, 1121
BstMBI	GATC	5 cut(s)	72, 151, 773, 1023, 1106
BstMWI	GCNNNNNNNGC	3 cut(s)	47, 473, 834
BstNI	CCWGG	2 cut(s)	513, 1007
BstNSI	RCATGY	1 cut(s)	547
BstSCI	CCNGG	3 cut(s)	511, 1005, 1130
BstSFI	CTRYAG	2 cut(s)	332, 841
BstUI	CGCG	2 cut(s)	327, 1118
BstV1I	GCAGC	2 cut(s)	28, 451
BstX2I	RGATCY	2 cut(s)	72, 151
BstXI	CCANNNNNNTGG	1 cut(s)	400
BstYI	RGATCY	2 cut(s)	72, 151
Bsu15I	ATCGAT	1 cut(s)	759
BsuRI	GGCC	1 cut(s)	295
BsuTUI	ATCGAT	1 cut(s)	759
BtgI	CCRYGG	3 cut(s)	30, 195, 1098
BtsCI	GGATG	2 cut(s)	405, 868
BtsIMutI	CAGTG	1 cut(s)	285
Cac8I	GCNNGC	4 cut(s)	48, 336, 462, 826
CfoI	GCGC	5 cut(s)	46, 489, 652, 1120, 1150
Cfr13I	GGNCC	1 cut(s)	293
ClaI	ATCGAT	1 cut(s)	759
Csp6I	GTAC	4 cut(s)	289, 426, 769, 1060
CviAII	CATG	5 cut(s)	31, 445, 544, 1099, 1141
CviQI	GTAC	4 cut(s)	289, 426, 769, 1060
DdeI	CTNAG	3 cut(s)	69, 528, 1125
DpnI	GATC	5 cut(s)	74, 153, 775, 1025, 1108
DpnII	GATC	5 cut(s)	72, 151, 773, 1023, 1106
Eam1104I	CTCTTC	1 cut(s)	246
EarI	CTCTTC	1 cut(s)	246
Eco130I	CCWWGG	4 cut(s)	30, 477, 519, 1098
Eco31I	GGTCTC	1 cut(s)	1121
Eco57I	CTGAAG	2 cut(s)	36, 254
EcoRI	GAATTC	1 cut(s)	181
EcoRII	CCWGG	2 cut(s)	511, 1005
EcoT14I	CCWWGG	4 cut(s)	30, 477, 519, 1098
ErhI	CCWWGG	4 cut(s)	30, 477, 519, 1098
Esp3I	CGTCTC	1 cut(s)	127
FaeI	CATG	5 cut(s)	34, 448, 547, 1102, 1144
FaqI	GGGAC	1 cut(s)	419
FatI	CATG	5 cut(s)	30, 444, 543, 1098, 1140
FblI	GTMKAC	1 cut(s)	96
Fnu4HI	GCNGC	3 cut(s)	42, 214, 465
FokI	GGATG	2 cut(s)	412, 875
Fsp4HI	GCNGC	3 cut(s)	42, 214, 465
FspBI	CTAG	1 cut(s)	431
FspI	TGCGCA	2 cut(s)	45, 488
GlaI	GCGC	5 cut(s)	45, 488, 651, 1119, 1149
GluI	GCNGC	3 cut(s)	42, 214, 465
GsuI	CTGGAG	1 cut(s)	666
HaeIII	GGCC	1 cut(s)	295
HapII	CCGG	2 cut(s)	210, 1131
HhaI	GCGC	5 cut(s)	46, 489, 652, 1120, 1150
Hin1II	CATG	5 cut(s)	34, 448, 547, 1102, 1144
Hin6I	GCGC	5 cut(s)	44, 487, 650, 1118, 1148
HinP1I	GCGC	5 cut(s)	44, 487, 650, 1118, 1148
HincII	GTYRAC	1 cut(s)	97
HindII	GTYRAC	1 cut(s)	97
HindIII	AAGCTT	1 cut(s)	48
HinfI	GANTC	2 cut(s)	492, 805
HpaII	CCGG	2 cut(s)	210, 1131
HphI	GGTGA	2 cut(s)	22, 250
Hpy166II	GTNNAC	3 cut(s)	97, 428, 634
Hpy188I	TCNGA	6 cut(s)	55, 72, 442, 922, 1023, 1126
Hpy188III	TCNNGA	4 cut(s)	22, 575, 714, 880
Hpy8I	GTNNAC	3 cut(s)	97, 428, 634
Hpy99I	CGWCG	1 cut(s)	98
HpyAV	CCTTC	4 cut(s)	229, 241, 1075, 1138
HpyCH4III	ACNGT	5 cut(s)	205, 244, 268, 280, 671
HpyCH4IV	ACGT	1 cut(s)	424
HpyCH4V	TGCA	7 cut(s)	147, 334, 359, 371, 406, 422, 824
HpyF10VI	GCNNNNNNNGC	3 cut(s)	47, 473, 834
HpyF3I	CTNAG	3 cut(s)	69, 528, 1125
HpySE526I	ACGT	1 cut(s)	424
Hsp92II	CATG	5 cut(s)	34, 448, 547, 1102, 1144
HspAI	GCGC	5 cut(s)	44, 487, 650, 1118, 1148
Kzo9I	GATC	5 cut(s)	72, 151, 773, 1023, 1106
LmnI	GCTCC	3 cut(s)	5, 65, 685
Lsp1109I	GCAGC	2 cut(s)	28, 451
LweI	GCATC	3 cut(s)	225, 575, 1079
MaeI	CTAG	1 cut(s)	431
MaeII	ACGT	1 cut(s)	424
MaeIII	GTNAC	2 cut(s)	238, 274
MalI	GATC	5 cut(s)	74, 153, 775, 1025, 1108
MbiI	CCGCTC	1 cut(s)	8
MboI	GATC	5 cut(s)	72, 151, 773, 1023, 1106
MboII	GAAGA	7 cut(s)	25, 72, 75, 263, 749, 752, 1042
MfeI	CAATTG	1 cut(s)	227
MflI	RGATCY	2 cut(s)	72, 151
MroXI	GAANNNNTTC	1 cut(s)	809
MseI	TTAA	4 cut(s)	159, 675, 729, 1136
MslI	CAYNNNNRTG	1 cut(s)	813
MspI	CCGG	2 cut(s)	210, 1131
MspR9I	CCNGG	3 cut(s)	513, 1007, 1132
MunI	CAATTG	1 cut(s)	227
Mva1269I	GAATGC	1 cut(s)	839
MvaI	CCWGG	2 cut(s)	513, 1007
MvnI	CGCG	2 cut(s)	327, 1118
MwoI	GCNNNNNNNGC	3 cut(s)	47, 473, 834
NciI	CCSGG	1 cut(s)	1132
NcoI	CCATGG	2 cut(s)	30, 1098
NdeII	GATC	5 cut(s)	72, 151, 773, 1023, 1106
NlaIII	CATG	5 cut(s)	34, 448, 547, 1102, 1144
NlaIV	GGNNCC	2 cut(s)	517, 525
NmuCI	GTSAC	2 cut(s)	238, 274
NsbI	TGCGCA	2 cut(s)	45, 488
NspI	RCATGY	1 cut(s)	547
PciI	ACATGT	1 cut(s)	543
PctI	GAATGC	1 cut(s)	839
PdmI	GAANNNNTTC	1 cut(s)	809
PfeI	GAWTC	2 cut(s)	492, 805
PflMI	CCANNNNNTGG	1 cut(s)	502
PkrI	GCNGC	3 cut(s)	43, 215, 466
Ppu21I	YACGTR	1 cut(s)	425
PscI	ACATGT	1 cut(s)	543
PsiI	TTATAA	1 cut(s)	609
Psp6I	CCWGG	2 cut(s)	511, 1005
PspGI	CCWGG	2 cut(s)	511, 1005
PspN4I	GGNNCC	2 cut(s)	517, 525
PspPI	GGNCC	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	336
PsuI	RGATCY	2 cut(s)	72, 151
RsaI	GTAC	4 cut(s)	290, 427, 770, 1061
RsaNI	GTAC	4 cut(s)	289, 426, 769, 1060
RseI	CAYNNNNRTG	1 cut(s)	813
SalI	GTCGAC	1 cut(s)	95
SaqAI	TTAA	4 cut(s)	159, 675, 729, 1136
SatI	GCNGC	3 cut(s)	42, 214, 465
Sau3AI	GATC	5 cut(s)	72, 151, 773, 1023, 1106
Sau96I	GGNCC	1 cut(s)	293
ScrFI	CCNGG	3 cut(s)	513, 1007, 1132
SfaNI	GCATC	3 cut(s)	225, 575, 1079
SfcI	CTRYAG	2 cut(s)	332, 841
SmiMI	CAYNNNNRTG	1 cut(s)	813
SmlI	CTYRAG	2 cut(s)	653, 880
SmoI	CTYRAG	2 cut(s)	653, 880
SpeI	ACTAGT	1 cut(s)	430
SsiI	CCGC	4 cut(s)	8, 213, 283, 1057
SspMI	CTAG	1 cut(s)	431
StyD4I	CCNGG	3 cut(s)	511, 1005, 1130
StyI	CCWWGG	4 cut(s)	30, 477, 519, 1098
TaaI	ACNGT	5 cut(s)	205, 244, 268, 280, 671
TaiI	ACGT	1 cut(s)	427
TaqI	TCGA	3 cut(s)	96, 759, 1032
TauI	GCSGC	1 cut(s)	216
TfiI	GAWTC	2 cut(s)	492, 805
Tru1I	TTAA	4 cut(s)	159, 675, 729, 1136
Tru9I	TTAA	4 cut(s)	159, 675, 729, 1136
TscAI	CASTG	1 cut(s)	285
TseFI	GTSAC	2 cut(s)	238, 274
TseI	GCWGC	2 cut(s)	41, 464
Tsp45I	GTSAC	2 cut(s)	238, 274
TspDTI	ATGAA	7 cut(s)	606, 609, 654, 745, 831, 846, 879
TspGWI	ACGGA	4 cut(s)	82, 184, 343, 786
TspRI	CASTG	1 cut(s)	285
Van91I	CCANNNNNTGG	1 cut(s)	502
XapI	RAATTY	5 cut(s)	172, 181, 373, 507, 578
XceI	RCATGY	1 cut(s)	547
XmiI	GTMKAC	1 cut(s)	96
XmnI	GAANNNNTTC	1 cut(s)	809
XspI	CTAG	1 cut(s)	431

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.