MD02G1046500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010815
Physical Location & Seq
Forward (+)
3710862 .. 3711542
681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1046500.v1.1.491

Sequence Viewer

Length: 681 bp
ATGTTGCTTAGAAGCTCCATTTACCACACCAAGAAGTTCTTCCAGAAAACCCTAGGGAGCTTGAAGAACTTCTTCTCTGACGGAAGCTACCAAAAGCTCCCAAAATCCCCTACTTTCCACAGCTACGACAACCCCTTTCCCCATGCTTCTGCTTCTACTTCTGTCGTTGACAACATGAATAATCTACACGCCACAACATACAAAGACTTGGACAAGTTGTACACCGACTTCACAGACCAATGGGATTCAAAAATCGAAAACATCAGGCGATCGTCGAACAAGAAGAAGCATCCCTCCACAACCGCCAAACCACATCTTCATAAAGATCATGTTCAAAACCCTACAAGCTTACCTCCACGGCCACTCAATTCACATCCTGCAGCTGCAGGTACTCCTCCTGCAAAGATTGAAACCTTTTCAAAGGAATCGAGGGCCGTGGGAAGGGGTTGTTTGGTGGAACAGAAGCTGAAGGAGTTGGAGATGTTGGACGTGAGCAATGTAGATCATGTTTTGGACATCGAAGAGGTTCTTCATTATTATTCTAGACTCACTTGTCCTGCCTATTTAGAAATTGTGGAAAAGTTTTTCATGGAAATGTATTCGGAGTTTTTCTCTCCGGCTGCAGCAACTCCGGCACGCAGCGTCAATTATGGCCTGAGGCCGAGGTCTGTGAGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.77

Weight (kDa)

9.18

Isoelectric Point (pI)

57.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010815)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24535
fragaria_vesca FvH4_1g04790
malus_domestica MD02G1046500.v1.1 MD15G1184700.v1.1
prunus_persica Prupe.7G231300_v2.0.a1
pyrus_communis pycom02g03900 pycom15g16620
rosa_chinensis RchiOBHm_Chr2g0090421
rosa_laevigata RLG00000016105 RLG00000016107
rosa_multiflora Rmu_sc0004514.1_g000014
rosa_roxburghii Rroxscaffold_2G00151170
rosa_rugosa Rorug02G0008000
rosa_samantha Rh2AG053300 Rh2BG052600 Rh2CG054400 Rh2CG054700 Rh2DG053300 Rh2DG053500
rosa_wichuraiana Rw2G004380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 377
AciI CCGC 1 cut(s) 303
AcoI YGGCCR 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 488
AfaI GTAC 2 cut(s) 221, 391
AfiI CCNNNNNNNGG 1 cut(s) 441
AgsI TTSAA 5 cut(s) 64, 249, 335, 410, 420
AjiI CACGTC 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 8 cut(s) 15, 60, 87, 97, 123, 348, 383, 466
AluI AGCT 8 cut(s) 15, 60, 87, 97, 123, 348, 383, 466
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 4 cut(s) 359, 432, 652, 659
ApeKI GCWGC 5 cut(s) 380, 383, 620, 623, 639
Asp700I GAANNNNTTC 4 cut(s) 38, 68, 71, 525
AspA2I CCTAGG 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 432, 675
AvaII GGWCC 1 cut(s) 675
AvrII CCTAGG 1 cut(s) 52
AxyI CCTNAGG 1 cut(s) 656
BarI GAAGNNNNNNTAC 2 cut(s) 212, 244
BbvI GCAGC 5 cut(s) 370, 392, 607, 635, 651
BceAI ACGGC 2 cut(s) 374, 419
BfaI CTAG 2 cut(s) 53, 543
BfmI CTRYAG 3 cut(s) 378, 384, 621
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 5 cut(s) 381, 384, 621, 624, 640
BlnI CCTAGG 1 cut(s) 52
BlsI GCNGC 5 cut(s) 382, 385, 622, 625, 641
Bme18I GGWCC 1 cut(s) 675
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 432, 675
BmsI GCATC 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BsaJI CCNNGG 4 cut(s) 52, 356, 435, 662
Bsc4I CCNNNNNNNGG 1 cut(s) 441
Bse21I CCTNAGG 1 cut(s) 656
Bse3DI GCAATG 1 cut(s) 502
BseDI CCNNGG 4 cut(s) 52, 356, 435, 662
BseGI GGATG 2 cut(s) 289, 373
BseLI CCNNNNNNNGG 1 cut(s) 441
BseMI GCAATG 1 cut(s) 502
BseMII CTCAG 1 cut(s) 647
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 5 cut(s) 370, 392, 607, 635, 651
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 4 cut(s) 361, 434, 654, 661
BsiEI CGRYCG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 617, 632
BslI CCNNNNNNNGG 1 cut(s) 441
BsnI GGCC 4 cut(s) 361, 434, 654, 661
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 3 cut(s) 269, 325, 502
BspACI CCGC 1 cut(s) 303
BspANI GGCC 4 cut(s) 361, 434, 654, 661
BspCNI CTCAG 1 cut(s) 648
BspMAI CTGCAG 3 cut(s) 382, 388, 625
BspMI ACCTGC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 4 cut(s) 52, 356, 435, 662
BssMI GATC 3 cut(s) 269, 325, 502
BssT1I CCWWGG 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 516
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 2 cut(s) 8, 656
BstDSI CCRYGG 2 cut(s) 356, 435
BstF5I GGATG 2 cut(s) 289, 373
BstKTI GATC 3 cut(s) 272, 328, 505
BstMBI GATC 3 cut(s) 269, 325, 502
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstSFI CTRYAG 3 cut(s) 378, 384, 621
BstV1I GCAGC 5 cut(s) 370, 392, 607, 635, 651
Bsu36I CCTNAGG 1 cut(s) 656
BsuRI GGCC 4 cut(s) 361, 434, 654, 661
BtgI CCRYGG 2 cut(s) 356, 435
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 2 cut(s) 289, 373
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 637
CaiI CAGNNNCTG 1 cut(s) 466
Cfr13I GGNCC 2 cut(s) 432, 675
CseI GACGC 1 cut(s) 631
Csp6I GTAC 2 cut(s) 220, 390
CviAII CATG 5 cut(s) 143, 175, 329, 506, 589
CviQI GTAC 2 cut(s) 220, 390
DdeI CTNAG 2 cut(s) 8, 656
DpnI GATC 3 cut(s) 271, 327, 504
DpnII GATC 3 cut(s) 269, 325, 502
DrdI GACNNNNNNGTC 1 cut(s) 552
DseDI GACNNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 488
Eco81I CCTNAGG 1 cut(s) 656
EcoO109I RGGNCCY 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 5 cut(s) 146, 178, 332, 509, 592
FaiI YATR 8 cut(s) 144, 176, 199, 321, 330, 507, 590, 651
FalI AAGNNNNNCTT 6 cut(s) 23, 55, 56, 88, 513, 545
FatI CATG 5 cut(s) 142, 174, 328, 505, 588
Fnu4HI GCNGC 5 cut(s) 381, 384, 621, 624, 640
FokI GGATG 2 cut(s) 276, 360
Fsp4HI GCNGC 5 cut(s) 381, 384, 621, 624, 640
FspBI CTAG 2 cut(s) 53, 543
GluI GCNGC 5 cut(s) 381, 384, 621, 624, 640
HaeIII GGCC 4 cut(s) 361, 434, 654, 661
HapII CCGG 2 cut(s) 617, 632
HgaI GACGC 1 cut(s) 631
Hin1II CATG 5 cut(s) 146, 178, 332, 509, 592
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 3 cut(s) 245, 425, 546
HpaII CCGG 2 cut(s) 617, 632
Hpy166II GTNNAC 2 cut(s) 169, 222
Hpy188I TCNGA 2 cut(s) 79, 604
Hpy188III TCNNGA 2 cut(s) 43, 543
Hpy8I GTNNAC 2 cut(s) 169, 222
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 435, 463
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 4 cut(s) 380, 386, 401, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 2 cut(s) 8, 656
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 5 cut(s) 146, 178, 332, 509, 592
Kzo9I GATC 3 cut(s) 269, 325, 502
LmnI GCTCC 3 cut(s) 20, 57, 102
LpnPI CCDG 9 cut(s) 56, 250, 372, 390, 411, 570, 630, 645, 668
Lsp1109I GCAGC 5 cut(s) 370, 392, 607, 635, 651
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 53, 543
MaeII ACGT 1 cut(s) 489
MalI GATC 3 cut(s) 271, 327, 504
MboI GATC 3 cut(s) 269, 325, 502
MboII GAAGA 7 cut(s) 31, 64, 76, 295, 308, 521, 533
MluCI AATT 3 cut(s) 367, 570, 646
MlyI GAGTC 1 cut(s) 540
MmeI TCCRAC 2 cut(s) 456, 465
MnlI CCTC 8 cut(s) 304, 363, 405, 423, 517, 651, 657, 666
MroXI GAANNNNTTC 4 cut(s) 38, 68, 71, 525
MslI CAYNNNNRTG 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 617, 632
MwoI GCNNNNNNNGC 1 cut(s) 632
NdeII GATC 3 cut(s) 269, 325, 502
NlaIII CATG 5 cut(s) 146, 178, 332, 509, 592
PdmI GAANNNNTTC 4 cut(s) 38, 68, 71, 525
PfeI GAWTC 2 cut(s) 245, 425
PkrI GCNGC 5 cut(s) 382, 385, 622, 625, 641
Ple19I CGATCG 1 cut(s) 272
PleI GAGTC 1 cut(s) 540
PpsI GAGTC 1 cut(s) 540
PpuMI RGGWCCY 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 675
PspPI GGNCC 2 cut(s) 432, 675
PspPPI RGGWCCY 1 cut(s) 675
PstI CTGCAG 3 cut(s) 382, 388, 625
PstNI CAGNNNCTG 1 cut(s) 466
PvuI CGATCG 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 221, 391
RsaNI GTAC 2 cut(s) 220, 390
RseI CAYNNNNRTG 1 cut(s) 593
SatI GCNGC 5 cut(s) 381, 384, 621, 624, 640
Sau3AI GATC 3 cut(s) 269, 325, 502
Sau96I GGNCC 2 cut(s) 432, 675
SchI GAGTC 1 cut(s) 540
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 3 cut(s) 378, 384, 621
SinI GGWCC 1 cut(s) 675
SmiMI CAYNNNNRTG 1 cut(s) 593
Sse9I AATT 3 cut(s) 367, 570, 646
SsiI CCGC 1 cut(s) 303
SspMI CTAG 2 cut(s) 53, 543
StyI CCWWGG 1 cut(s) 52
TaiI ACGT 1 cut(s) 492
TaqI TCGA 4 cut(s) 255, 275, 428, 519
TasI AATT 3 cut(s) 367, 570, 646
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 2 cut(s) 245, 425
TseI GCWGC 5 cut(s) 380, 383, 620, 623, 639
TspDTI ATGAA 4 cut(s) 191, 308, 521, 577
TspGWI ACGGA 1 cut(s) 96
VpaK11BI GGWCC 1 cut(s) 675
XbaI TCTAGA 1 cut(s) 542
XmaJI CCTAGG 1 cut(s) 52
XmnI GAANNNNTTC 4 cut(s) 38, 68, 71, 525
XspI CTAG 2 cut(s) 53, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.