Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009958

Physical Location & Seq

Genomic Coordinates Reverse (-)

5328769 .. 5330006

Gene Structure 1238 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1065700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGGGGGTAAAGAAAGTAGTTGGAAGGGGAAGTGCGGTAAAGGATTATCAAAGTGATTACCGTGGGAGGGACAGAAGATTGGAGGAAGCCAATACAGAAGGAGATGCAGAAGAAGAAGAAGTAGGAGAAGAAGAAGAAATGGAAGCGGTTGATGATCCAAGGTGGGATCATATTCAAAACAAGCTCAGGGGAATGGAAGATGTAAAACCTGGACGTGAGAGACCTGAGTTTCGAAGGTGGAATAGGCAGGAAGATTGGGGTAAGAAGACATGGAACGAGGCTACTGAATCATCCGTGCCTAGGATGATTGGCCAAAGTGTTTATGGTGTTGGTCCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAATTTTATGCATTGTATATTCAAGAAGGGATGGATTTGAGCAAAAATAATAGGAAGAAGAAGGACAAGAAGGGCTTTGAGAGAATTTTACTGATGGCTGACAAGATTGGGTTAAACGTAAAGGATGTCTCTAAGCATGATCTTAATATGGCTACTGATAATCGACCTCATCAGGGTCTGGTTCTAGATACCTCTCCGTTGGAGATGGTGAAAATTAACGAATTGGAACCTGTTTCAGTTGAGGAAGATAAGGGTTCTCTCTGGATTGCTTTGGATGAGGTTACAGATCCTCAGAATGTGGGGGCAATCATCAGGTCGGCTTACTTCTTTGGAGCTTCTGGGGTGGTGTTATGTGCCAAGAATTCAGCCCCACTGAGTGGCGTTGTGAGCAAATCAAGTGCAGGCTCACTTGAGTTGATGGAGTTGAGATACTGCAAGAATATGATGCAGTTCCTAACATCCTCGGCTGAAAATGGTTGGCGGGTTCTTGGAGGTTCTGTTTCTTCCAAGGCTTTACCTTTGAACGAGGTTGCACCTAGTGTGCCAACAATTCTTGTTCTGGGAAGTGAGGGCACTGGGTTGAGACCTTTGGTGGAAAGATCTTGCACTGATTTGATTAGAATCCCGGGGAATATTCCCGTGGAAATTACTGCTGGTGGAGTTGATGATACTGAAACCGTGGAAGGGAAACATGCATGCTCAGCTGAAGAGTTCCGATCACTTTTGGCCGTGGAGAGCTTAAATGTCAGTGTTGCAACTGGTGTGCTGCTTCATCACTTAACCGGAAACAATAACAATGAGAATGTCCATGCAGTTAATCAGCAGACTGACGAACTTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008649 GO:0008757 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016435 GO:0016740 GO:0016741 GO:0022613 GO:0031167 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0070039 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:0140102 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase SpoU_sub_bind

Protein Analysis

403

Amino Acids

44.26

Weight (kDa)

5.05

Isoelectric Point (pI)

47.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SpoU_sub_bind	PF08032	107 - 191	2.8e-10	RNA 2'-O ribose methyltransferase substrate binding
SpoU_methylase	PF00588	209 - 333	1.7e-25	SpoU rRNA Methylase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009958)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19870
fragaria_vesca	FvH4_1g06150 FvH4_1g06190
malus_domestica	MD02G1065700.v1.1 MD15G1196600.v1.1
prunus_persica	Prupe.7G219800_v2.0.a1 Prupe.7G219800_v2.0.a1
pyrus_communis	pycom02g05180 pycom02g05220 pycom15g17570
rosa_chinensis	RchiOBHm_Chr2g0091911
rosa_laevigata	RLG00000016260
rosa_multiflora	Rmu_sc0031557.1_g000002 Rmu_sc0031558.1_g000001
rosa_roxburghii	Rroxscaffold_2G00149620
rosa_rugosa	Rorug02G0021600
rosa_samantha	Rh2AG067200 Rh2BG066400 Rh2CG067700 Rh2DG066300
rosa_wichuraiana	Rw2G005380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	743
AciI	CCGC	3 cut(s)	35, 146, 847
AclWI	GGATC	3 cut(s)	149, 174, 647
AcoI	YGGCCR	2 cut(s)	310, 1092
AcsI	RAATTY	3 cut(s)	368, 450, 727
AcuI	CTGAAG	1 cut(s)	1092
AdeI	CACNNNGTG	2 cut(s)	743, 905
AfiI	CCNNNNNNNGG	5 cut(s)	67, 300, 539, 743, 1050
AgsI	TTSAA	3 cut(s)	176, 389, 889
AjiI	CACGTC	1 cut(s)	215
AjnI	CCWGG	1 cut(s)	208
AluBI	AGCT	5 cut(s)	184, 356, 701, 1070, 1104
AluI	AGCT	5 cut(s)	184, 356, 701, 1070, 1104
Alw26I	GTCTC	3 cut(s)	214, 499, 943
AlwI	GGATC	3 cut(s)	149, 174, 647
AlwNI	CAGNNNCTG	1 cut(s)	544
Ama87I	CYCGRG	1 cut(s)	991
AoxI	GGCC	2 cut(s)	310, 1092
ApeKI	GCWGC	2 cut(s)	344, 1132
ApoI	RAATTY	3 cut(s)	368, 450, 727
ArsI	GACNNNNNNTTYG	2 cut(s)	213, 245
AspA2I	CCTAGG	1 cut(s)	299
AspS9I	GGNCC	1 cut(s)	332
AsuC2I	CCSGG	2 cut(s)	992, 993
AsuHPI	GGTGA	1 cut(s)	586
AsuII	TTCGAA	1 cut(s)	232
AvaI	CYCGRG	1 cut(s)	991
AvaII	GGWCC	1 cut(s)	332
AvrII	CCTAGG	1 cut(s)	299
BaeGI	GKGCMC	1 cut(s)	941
BalI	TGGCCA	1 cut(s)	312
BbsI	GAAGAC	1 cut(s)	272
BbvI	GCAGC	2 cut(s)	331, 1119
BccI	CCATC	4 cut(s)	391, 454, 565, 778
BceAI	ACGGC	1 cut(s)	1079
BciT130I	CCWGG	1 cut(s)	210
BcnI	CCSGG	2 cut(s)	992, 993
BcoDI	GTCTC	3 cut(s)	214, 499, 943
BfaI	CTAG	3 cut(s)	300, 551, 903
BglII	AGATCT	1 cut(s)	965
BisI	GCNGC	2 cut(s)	345, 1133
BlnI	CCTAGG	1 cut(s)	299
BlpI	GCTNAGC	1 cut(s)	1066
BlsI	GCNGC	2 cut(s)	346, 1134
Bme1390I	CCNGG	3 cut(s)	210, 992, 993
Bme18I	GGWCC	1 cut(s)	332
BmeT110I	CYCGRG	1 cut(s)	991
BmgBI	CACGTC	1 cut(s)	215
BmgT120I	GGNCC	1 cut(s)	332
BmiI	GGNNCC	1 cut(s)	594
BmrFI	CCNGG	3 cut(s)	210, 992, 993
BmrI	ACTGGG	1 cut(s)	951
BmsI	GCATC	2 cut(s)	94, 801
BmuI	ACTGGG	1 cut(s)	951
BpiI	GAAGAC	1 cut(s)	272
Bpu10I	CCTNAGC	1 cut(s)	185
Bpu1102I	GCTNAGC	1 cut(s)	1066
Bpu14I	TTCGAA	1 cut(s)	232
BpuEI	CTTGAG	1 cut(s)	797
BpuMI	CCSGG	2 cut(s)	992, 993
BsaBI	GATNNNNATC	1 cut(s)	986
BsaI	GGTCTC	2 cut(s)	214, 943
BsaWI	WCCGGW	1 cut(s)	1148
BsaXI	ACNNNNNCTCC	2 cut(s)	849, 879
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 300, 539, 743, 1050
Bse1I	ACTGG	3 cut(s)	335, 946, 1129
Bse8I	GATNNNNATC	1 cut(s)	986
BseBI	CCWGG	1 cut(s)	210
BseGI	GGATG	6 cut(s)	290, 309, 402, 496, 646, 824
BseJI	GATNNNNATC	1 cut(s)	986
BseLI	CCNNNNNNNGG	5 cut(s)	67, 300, 539, 743, 1050
BseMII	CTCAG	5 cut(s)	199, 216, 671, 731, 1080
BseNI	ACTGG	3 cut(s)	335, 946, 1129
BseSI	GKGCMC	1 cut(s)	941
BseXI	GCAGC	2 cut(s)	331, 1119
BsgI	GTGCAG	1 cut(s)	786
BshFI	GGCC	2 cut(s)	312, 1094
BsiHKCI	CYCGRG	1 cut(s)	991
BsiSI	CCGG	2 cut(s)	992, 1149
BslFI	GGGAC	1 cut(s)	83
BslI	CCNNNNNNNGG	5 cut(s)	67, 300, 539, 743, 1050
BsmAI	GTCTC	3 cut(s)	214, 499, 943
BsmFI	GGGAC	1 cut(s)	83
BsnI	GGCC	2 cut(s)	312, 1094
Bso31I	GGTCTC	2 cut(s)	214, 943
BsoBI	CYCGRG	1 cut(s)	991
Bsp119I	TTCGAA	1 cut(s)	232
Bsp1286I	GDGCHC	1 cut(s)	941
Bsp143I	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
Bsp1720I	GCTNAGC	1 cut(s)	1066
BspACI	CCGC	3 cut(s)	35, 146, 847
BspANI	GGCC	2 cut(s)	312, 1094
BspCNI	CTCAG	5 cut(s)	198, 217, 670, 732, 1079
BspLI	GGNNCC	1 cut(s)	594
BspPI	GGATC	3 cut(s)	149, 174, 647
BspT104I	TTCGAA	1 cut(s)	232
BspTNI	GGTCTC	2 cut(s)	214, 943
BsrI	ACTGG	3 cut(s)	335, 946, 1129
BssMI	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
BssT1I	CCWWGG	3 cut(s)	158, 299, 873
Bst2UI	CCWGG	1 cut(s)	210
Bst4CI	ACNGT	2 cut(s)	62, 1045
Bst6I	CTCTTC	2 cut(s)	358, 1068
BstBI	TTCGAA	1 cut(s)	232
BstC8I	GCNNGC	2 cut(s)	769, 1063
BstDEI	CTNAG	6 cut(s)	185, 225, 498, 657, 740, 1066
BstDSI	CCRYGG	4 cut(s)	61, 1005, 1044, 1095
BstF5I	GGATG	6 cut(s)	290, 309, 402, 496, 646, 824
BstKTI	GATC	6 cut(s)	157, 169, 508, 655, 968, 1085
BstMAI	GTCTC	3 cut(s)	214, 499, 943
BstMBI	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
BstMWI	GCNNNNNNNGC	3 cut(s)	353, 753, 1067
BstNI	CCWGG	1 cut(s)	210
BstNSI	RCATGY	2 cut(s)	1061, 1065
BstSCI	CCNGG	3 cut(s)	208, 990, 991
BstSLI	GKGCMC	1 cut(s)	941
BstV1I	GCAGC	2 cut(s)	331, 1119
BstV2I	GAAGAC	1 cut(s)	272
BstX2I	RGATCY	2 cut(s)	652, 965
BstYI	RGATCY	2 cut(s)	652, 965
BsuRI	GGCC	2 cut(s)	312, 1094
BtgI	CCRYGG	4 cut(s)	61, 1005, 1044, 1095
BtrI	CACGTC	1 cut(s)	215
BtsCI	GGATG	6 cut(s)	290, 309, 402, 496, 646, 824
BtsIMutI	CAGTG	4 cut(s)	737, 939, 972, 1120
Cac8I	GCNNGC	2 cut(s)	769, 1063
CaiI	CAGNNNCTG	1 cut(s)	544
Cfr13I	GGNCC	1 cut(s)	332
Cfr9I	CCCGGG	1 cut(s)	991
CviAII	CATG	5 cut(s)	270, 503, 1058, 1062, 1175
DdeI	CTNAG	6 cut(s)	185, 225, 498, 657, 740, 1066
DpnI	GATC	6 cut(s)	156, 168, 507, 654, 967, 1084
DpnII	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
DraIII	CACNNNGTG	2 cut(s)	743, 905
EaeI	YGGCCR	2 cut(s)	310, 1092
Eam1104I	CTCTTC	2 cut(s)	358, 1068
EarI	CTCTTC	2 cut(s)	358, 1068
Eco130I	CCWWGG	3 cut(s)	158, 299, 873
Eco31I	GGTCTC	2 cut(s)	214, 943
Eco47I	GGWCC	1 cut(s)	332
Eco57I	CTGAAG	1 cut(s)	1092
Eco88I	CYCGRG	1 cut(s)	991
EcoRI	GAATTC	1 cut(s)	727
EcoRII	CCWGG	1 cut(s)	208
EcoT14I	CCWWGG	3 cut(s)	158, 299, 873
EcoT22I	ATGCAT	2 cut(s)	379, 1063
ErhI	CCWWGG	3 cut(s)	158, 299, 873
FaeI	CATG	5 cut(s)	273, 506, 1061, 1065, 1178
FalI	AAGNNNNNCTT	2 cut(s)	425, 457
FaqI	GGGAC	1 cut(s)	83
FatI	CATG	5 cut(s)	269, 502, 1057, 1061, 1174
FauI	CCCGC	1 cut(s)	840
Fnu4HI	GCNGC	2 cut(s)	345, 1133
FokI	GGATG	6 cut(s)	277, 316, 409, 503, 653, 811
Fsp4HI	GCNGC	2 cut(s)	345, 1133
FspBI	CTAG	3 cut(s)	300, 551, 903
GluI	GCNGC	2 cut(s)	345, 1133
HaeIII	GGCC	2 cut(s)	312, 1094
HapII	CCGG	2 cut(s)	992, 1149
Hin1II	CATG	5 cut(s)	273, 506, 1061, 1065, 1178
HinfI	GANTC	2 cut(s)	287, 987
HpaII	CCGG	2 cut(s)	992, 1149
HphI	GGTGA	1 cut(s)	586
Hpy188I	TCNGA	2 cut(s)	660, 1082
Hpy188III	TCNNGA	3 cut(s)	389, 551, 628
HpyAV	CCTTC	7 cut(s)	18, 92, 228, 386, 421, 430, 1043
HpyCH4III	ACNGT	2 cut(s)	62, 1045
HpyCH4IV	ACGT	2 cut(s)	214, 483
HpyF10VI	GCNNNNNNNGC	3 cut(s)	353, 753, 1067
HpyF3I	CTNAG	6 cut(s)	185, 225, 498, 657, 740, 1066
HpySE526I	ACGT	2 cut(s)	214, 483
Hsp92II	CATG	5 cut(s)	273, 506, 1061, 1065, 1178
Kzo9I	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
LmnI	GCTCC	1 cut(s)	698
Lsp1109I	GCAGC	2 cut(s)	331, 1119
LweI	GCATC	2 cut(s)	94, 801
MaeI	CTAG	3 cut(s)	300, 551, 903
MaeII	ACGT	2 cut(s)	214, 483
MaeIII	GTNAC	1 cut(s)	646
MalI	GATC	6 cut(s)	156, 168, 507, 654, 967, 1084
MboI	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
MflI	RGATCY	2 cut(s)	652, 965
MhlI	GDGCHC	1 cut(s)	941
MlsI	TGGCCA	1 cut(s)	312
MluCI	AATT	7 cut(s)	368, 450, 579, 587, 727, 915, 1011
MluNI	TGGCCA	1 cut(s)	312
MmeI	TCCRAC	1 cut(s)	546
Mox20I	TGGCCA	1 cut(s)	312
Mph1103I	ATGCAT	2 cut(s)	379, 1063
MscI	TGGCCA	1 cut(s)	312
MseI	TTAA	6 cut(s)	479, 510, 582, 1106, 1145, 1182
Msp20I	TGGCCA	1 cut(s)	312
MspA1I	CMGCKG	2 cut(s)	356, 1070
MspI	CCGG	2 cut(s)	992, 1149
MspR9I	CCNGG	3 cut(s)	210, 992, 993
MvaI	CCWGG	1 cut(s)	210
MwoI	GCNNNNNNNGC	3 cut(s)	353, 753, 1067
NciI	CCSGG	2 cut(s)	992, 993
NdeII	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
NlaIII	CATG	5 cut(s)	273, 506, 1061, 1065, 1178
NlaIV	GGNNCC	1 cut(s)	594
NmeAIII	GCCGAG	1 cut(s)	809
NsiI	ATGCAT	2 cut(s)	379, 1063
NspI	RCATGY	2 cut(s)	1061, 1065
NspV	TTCGAA	1 cut(s)	232
PaeI	GCATGC	1 cut(s)	1065
PfeI	GAWTC	2 cut(s)	287, 987
PflMI	CCANNNNNTGG	1 cut(s)	743
PkrI	GCNGC	2 cut(s)	346, 1134
Psp6I	CCWGG	1 cut(s)	208
PspGI	CCWGG	1 cut(s)	208
PspN4I	GGNNCC	1 cut(s)	594
PspPI	GGNCC	1 cut(s)	332
PstNI	CAGNNNCTG	1 cut(s)	544
PsuI	RGATCY	2 cut(s)	652, 965
PvuII	CAGCTG	2 cut(s)	356, 1070
SaqAI	TTAA	6 cut(s)	479, 510, 582, 1106, 1145, 1182
SatI	GCNGC	2 cut(s)	345, 1133
Sau3AI	GATC	6 cut(s)	154, 166, 505, 652, 965, 1082
Sau96I	GGNCC	1 cut(s)	332
ScrFI	CCNGG	3 cut(s)	210, 992, 993
SduI	GDGCHC	1 cut(s)	941
SfaNI	GCATC	2 cut(s)	94, 801
SfuI	TTCGAA	1 cut(s)	232
SinI	GGWCC	1 cut(s)	332
SmaI	CCCGGG	1 cut(s)	993
SmlI	CTYRAG	1 cut(s)	776
SmoI	CTYRAG	1 cut(s)	776
SphI	GCATGC	1 cut(s)	1065
Sse9I	AATT	7 cut(s)	368, 450, 579, 587, 727, 915, 1011
SsiI	CCGC	3 cut(s)	35, 146, 847
SspI	AATATT	1 cut(s)	1000
SspMI	CTAG	3 cut(s)	300, 551, 903
StyD4I	CCNGG	3 cut(s)	208, 990, 991
StyI	CCWWGG	3 cut(s)	158, 299, 873
TaaI	ACNGT	2 cut(s)	62, 1045
TaiI	ACGT	2 cut(s)	217, 486
TaqI	TCGA	2 cut(s)	232, 529
TasI	AATT	7 cut(s)	368, 450, 579, 587, 727, 915, 1011
TfiI	GAWTC	2 cut(s)	287, 987
Tru1I	TTAA	6 cut(s)	479, 510, 582, 1106, 1145, 1182
Tru9I	TTAA	6 cut(s)	479, 510, 582, 1106, 1145, 1182
TscAI	CASTG	4 cut(s)	744, 946, 979, 1120
TseI	GCWGC	2 cut(s)	344, 1132
TspDTI	ATGAA	1 cut(s)	1127
TspGWI	ACGGA	2 cut(s)	283, 552
TspMI	CCCGGG	1 cut(s)	991
TspRI	CASTG	4 cut(s)	744, 946, 979, 1120
Van91I	CCANNNNNTGG	1 cut(s)	743
VpaK11BI	GGWCC	1 cut(s)	332
XapI	RAATTY	3 cut(s)	368, 450, 727
XbaI	TCTAGA	1 cut(s)	550
XceI	RCATGY	2 cut(s)	1061, 1065
XcmI	CCANNNNNNNNNTGG	1 cut(s)	320
XmaI	CCCGGG	1 cut(s)	991
XmaJI	CCTAGG	1 cut(s)	299
XspI	CTAG	3 cut(s)	300, 551, 903
Zsp2I	ATGCAT	2 cut(s)	379, 1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.