MD02G1074800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012559
Physical Location & Seq
Reverse (-)
5975817 .. 5976518
702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1074800.v1.1.491

Sequence Viewer

Length: 702 bp
ATGTATGAATTCTTGGGTTGCAGTCTCGTTGATATGTATTCGAAGTGTGGGATGGCAGTCGATGCAGTAAAAGCATTCAGGATGATTGAAAAACCTGATGTAGTGGCCTGGAGTGCAGTTATCTCTTGCCTTGAGCAACAAGGGCAATGGAAAGAAGTGCCTCAGCTATTTCGTGAAATGATAAGCACAGGCATCTCACCAAATAAATTTAACCTTTCTAGTATCATTTGTGCTGCCACTGATCTCCGAGACCTTCATTTTGGTGAAAGTATCCATGCTTTTGTGTGGAAATATGGTTGTGAAATTGATATTTCAGTCAGCGATGCTCTAATCACAATGTATATTAAAAGTGGGCGTTTTCTAGATGGTACTCAGATTTTTGAGGCTATGACAAACCCTGATTTGATTTCATGGAATGCCTTTTTATCCGGAGCGCACAATCATGAATTATCTGACCTAGGGCCAAGAGTTGTCCACCAAATGCTTGTCGAAGGTTTGAAGCCCAACATGTACTCATTCATTAGTGTTTTAAGGTCTTGCTCTATCCTCTTGGATGTAGGCCTTGGAAAACAAGTACATTCTCATATTATCAAAACTAACCTCGATGATAATGACTTTCTTGGGACAGCTCTCATTGACATGTATGCCAAAGGCAGGTTCGTGGAAGATGCTGTGAAAGCTTTCGATAGATTGAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 map00190 map01100 map04714
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

234

Amino Acids

26.07

Weight (kDa)

5.31

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 32 - 76 1.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 645
AccIII TCCGGA 1 cut(s) 428
AcsI RAATTY 2 cut(s) 8, 206
AfaI GTAC 3 cut(s) 370, 512, 576
AfiI CCNNNNNNNGG 1 cut(s) 654
AflIII ACRYGT 2 cut(s) 507, 639
AgsI TTSAA 2 cut(s) 89, 499
AjnI CCWGG 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 3 cut(s) 166, 629, 680
AluI AGCT 3 cut(s) 166, 629, 680
Alw26I GTCTC 2 cut(s) 29, 243
Aor13HI TCCGGA 1 cut(s) 428
AoxI GGCC 3 cut(s) 105, 461, 559
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 2 cut(s) 8, 206
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
Asp700I GAANNNNTTC 1 cut(s) 680
AspA2I CCTAGG 1 cut(s) 457
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 2 cut(s) 189, 275
AsuII TTCGAA 1 cut(s) 41
AvrII CCTAGG 1 cut(s) 457
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 220
BccI CCATC 2 cut(s) 46, 359
BciT130I CCWGG 1 cut(s) 109
BciVI GTATCC 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 29, 243
BfaI CTAG 3 cut(s) 219, 362, 458
BfuAI ACCTGC 1 cut(s) 645
BfuI GTATCC 1 cut(s) 281
BisI GCNGC 1 cut(s) 234
BlnI CCTAGG 1 cut(s) 457
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 461
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 4 cut(s) 52, 201, 313, 658
BpmI CTGGAG 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 457, 562
BsaWI WCCGGW 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse3DI GCAATG 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 428
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 457, 562
BseGI GGATG 3 cut(s) 57, 87, 559
BseLI CCNNNNNNNGG 1 cut(s) 654
BseMI GCAATG 1 cut(s) 152
BseMII CTCAG 2 cut(s) 176, 386
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 3 cut(s) 107, 463, 561
BsiSI CCGG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 2 cut(s) 29, 243
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 2 cut(s) 74, 421
BsnI GGCC 3 cut(s) 107, 463, 561
Bso31I GGTCTC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 428
Bsp143I GATC 1 cut(s) 241
BspANI GGCC 3 cut(s) 107, 463, 561
BspCNI CTCAG 2 cut(s) 175, 385
BspEI TCCGGA 1 cut(s) 428
BspHI TCATGA 1 cut(s) 442
BspMI ACCTGC 1 cut(s) 645
BspT104I TTCGAA 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 457, 562
BssMI GATC 1 cut(s) 241
BssT1I CCWWGG 2 cut(s) 457, 562
Bst2UI CCWGG 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 162, 372
BstF5I GGATG 3 cut(s) 57, 87, 559
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 1 cut(s) 244
BstMAI GTCTC 2 cut(s) 29, 243
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 5 cut(s) 62, 71, 113, 142, 677
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 2 cut(s) 511, 643
BstSCI CCNGG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 220
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 3 cut(s) 107, 463, 561
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 3 cut(s) 57, 87, 559
BtsIMutI CAGTG 1 cut(s) 237
BveI ACCTGC 1 cut(s) 645
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 3 cut(s) 369, 511, 575
CviAII CATG 5 cut(s) 275, 411, 443, 508, 640
CviJI RGCY 8 cut(s) 107, 166, 386, 463, 502, 561, 629, 680
CviKI_1 RGCY 8 cut(s) 107, 166, 386, 463, 502, 561, 629, 680
CviQI GTAC 3 cut(s) 369, 511, 575
DdeI CTNAG 2 cut(s) 162, 372
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 457, 562
Eco147I AGGCCT 1 cut(s) 561
Eco31I GGTCTC 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 457, 562
ErhI CCWWGG 2 cut(s) 457, 562
FaeI CATG 5 cut(s) 278, 414, 446, 511, 643
FaqI GGGAC 1 cut(s) 637
FatI CATG 5 cut(s) 274, 410, 442, 507, 639
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 3 cut(s) 64, 94, 566
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 3 cut(s) 219, 362, 458
GlaI GCGC 1 cut(s) 435
GluI GCNGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 130
HaeIII GGCC 3 cut(s) 107, 463, 561
HapII CCGG 1 cut(s) 429
HhaI GCGC 1 cut(s) 436
Hin1II CATG 5 cut(s) 278, 414, 446, 511, 643
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 678
HpaII CCGG 1 cut(s) 429
HphI GGTGA 2 cut(s) 189, 275
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 3 cut(s) 248, 375, 454
Hpy188III TCNNGA 5 cut(s) 79, 173, 362, 429, 443
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 2 cut(s) 263, 485
HpyCH4V TGCA 3 cut(s) 21, 65, 116
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 71, 113, 142, 677
HpyF3I CTNAG 2 cut(s) 162, 372
Hsp92II CATG 5 cut(s) 278, 414, 446, 511, 643
HspAI GCGC 1 cut(s) 434
Kpn2I TCCGGA 1 cut(s) 428
Kzo9I GATC 1 cut(s) 241
LmnI GCTCC 1 cut(s) 431
LpnPI CCDG 8 cut(s) 64, 94, 108, 121, 174, 411, 442, 640
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 4 cut(s) 52, 201, 313, 658
MaeI CTAG 3 cut(s) 219, 362, 458
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 1 cut(s) 677
MluCI AATT 5 cut(s) 8, 206, 303, 446, 697
MnlI CCTC 4 cut(s) 171, 376, 557, 611
MroI TCCGGA 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 680
MseI TTAA 3 cut(s) 210, 345, 530
MslI CAYNNNNRTG 3 cut(s) 261, 441, 638
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 109
Mva1269I GAATGC 2 cut(s) 74, 421
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 5 cut(s) 62, 71, 113, 142, 677
NdeII GATC 1 cut(s) 241
NlaIII CATG 5 cut(s) 278, 414, 446, 511, 643
NspI RCATGY 2 cut(s) 511, 643
NspV TTCGAA 1 cut(s) 41
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 561
PciI ACATGT 2 cut(s) 507, 639
PctI GAATGC 2 cut(s) 74, 421
PdmI GAANNNNTTC 1 cut(s) 680
PkrI GCNGC 1 cut(s) 235
PscI ACATGT 2 cut(s) 507, 639
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspPI GGNCC 1 cut(s) 461
RsaI GTAC 3 cut(s) 370, 512, 576
RsaNI GTAC 3 cut(s) 369, 511, 575
RseI CAYNNNNRTG 3 cut(s) 261, 441, 638
SaqAI TTAA 3 cut(s) 210, 345, 530
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 109
SfaNI GCATC 4 cut(s) 52, 201, 313, 658
SfuI TTCGAA 1 cut(s) 41
SmiMI CAYNNNNRTG 3 cut(s) 261, 441, 638
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 5 cut(s) 8, 206, 303, 446, 697
SseBI AGGCCT 1 cut(s) 561
SspMI CTAG 3 cut(s) 219, 362, 458
StuI AGGCCT 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 2 cut(s) 457, 562
TaqI TCGA 5 cut(s) 41, 60, 489, 603, 684
TasI AATT 5 cut(s) 8, 206, 303, 446, 697
TatI WGTACW 2 cut(s) 510, 574
Tru1I TTAA 3 cut(s) 210, 345, 530
Tru9I TTAA 3 cut(s) 210, 345, 530
TscAI CASTG 1 cut(s) 244
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 5 cut(s) 21, 245, 399, 459, 508
TspRI CASTG 1 cut(s) 244
XapI RAATTY 2 cut(s) 8, 206
XbaI TCTAGA 1 cut(s) 361
XceI RCATGY 2 cut(s) 511, 643
XmaJI CCTAGG 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 680
XspI CTAG 3 cut(s) 219, 362, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.