Rw2G006940

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012559
Physical Location & Seq
Forward (+)
6680789 .. 6683900
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006940.1

Sequence Viewer

Length: 1377 bp
ATGAAGAAAGATGGTACAAGAGCAAATGACTTTTCGTTGGCTACTGGTTTGAAAGCGTGTTCTTTGTGCTTAGATTTAAGCTTTGGAAAGCAGTTGCATGCTGAAGCAGTGAAAGCGGGGTTCTTTTCCGATGGTTTTGTTGGATCTGCTCTCGTTGATCTTTATGCAAAATGTGGTGAGATGGAACTTGCCAATAGAGTGTTATTTTGTATGCCTGAGCAGAATGTTGTATCATGGAATGCCCTGCTAAATGGGTATGCTAAGGAAGGTGATGGAAATCAAGTTCTGAAATTGTTTTGCAAAATGACTGAATCAGATATGAGGTTAAGTAAGTTCACCTTGTCTACTGTCCTTAAAGGTTGTGCAAACATAGAAAATTTGAGAGTAGGTCGGATTGTGCATTCTTTGGTTGTCAAAGTTGGGTTTGAGGTTGATGAATTCCTGGGTTGCAGTCTTGTTGATATGTATTCTAAGTGTCGAATGGCAATTGATGCATTAAAAGTATTCAGGACGATTAAAGATCCTGATGTAGTGGCCTGGAGTGCTATTATCACTTGCCTTGATCAACAAAGGCAATACCAGGAAATGGCTCGACTATTTCGTTCAATGATAAGCACAGGCATCTCACCGAACCAATTTACCCTTTCTAGTATTATTAGTGCTGCCACTGATCTTGGAGACCTCCATTTTGGTGAGAGCATCCATGCTCTTATATGGAAATATGGCTATGAATCTGATCTTCCAGTCAGCAATGCTCTAATCACAATGTATATGAAAATTGGATGTGTGAGAAATGGTGCTCAGGTTTTTGAGGCAATGACAGAACATGATCTGATTTCATGGAATGCCCTTCTATCTGGAGCACACGACCTAGGTCCAAGAGTATTCCACCAAATGCTTGCTGAAGGTTGCAAGCCCAACATGTATTCCTTCATCAGTATTTTAAGATCCTGTTCTAGCCTCTTGGATGCAGACCTCGGGAAACAAGTGCATGCCCATATTGTCAAAACAAGCCTCCATGATAATGACTTTGTTGGGACGGCTCTCATTGACATGTATGCTAAAAGTAGGTTCTTAGATGATGCTGTTATAGCTTTCAGTAGATTGAGTAATCGAGATCTTTTCACTTGGACAGTCATCATTACTGGTTATGTACAAACTGATCAAGCAGAGAAAGCTGTTGCTTGTTTCAGTCAGATGCAACAAGAAGGAGTGAAGCCAAACGAGATTAAAGGTGCTGCACACTTTTTTGTTGGGGATGAAATCCTCGACACTGAGGCGCTTGACGTGACTTCAGAGTTGGATGGGAGATACAGTGCCTTAATAGAGGTCCAGGTTTGTGATCGGGTGGTGACAGAGCAGCGGCCACTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 map00190 map01100 map04714
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

458

Amino Acids

50.74

Weight (kDa)

5.43

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 123 2.8e-12 PPR repeat family
PPR PF01535 77 - 105 7.2e-07 PPR repeat
PPR_2 PF13041 175 - 221 1.6e-08 PPR repeat family
PPR_2 PF13041 373 - 409 1.6e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 586
AccI GTMKAC 1 cut(s) 344
AciI CCGC 2 cut(s) 116, 1363
AclWI GGATC 3 cut(s) 151, 515, 942
AcoI YGGCCR 1 cut(s) 1364
AcsI RAATTY 2 cut(s) 376, 437
AcuI CTGAAG 4 cut(s) 123, 924, 1278, 1355
AfaI GTAC 2 cut(s) 16, 1155
AfiI CCNNNNNNNGG 1 cut(s) 586
AflIII ACRYGT 2 cut(s) 921, 1053
AgsI TTSAA 2 cut(s) 52, 606
AjiI CACGTC 1 cut(s) 1288
AjnI CCWGG 4 cut(s) 441, 536, 579, 1332
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 3 cut(s) 81, 1094, 1178
AluI AGCT 3 cut(s) 81, 1094, 1178
Alw21I GWGCWC 2 cut(s) 802, 865
Alw26I GTCTC 1 cut(s) 672
AlwI GGATC 3 cut(s) 151, 515, 942
Ama87I CYCGRG 1 cut(s) 977
AoxI GGCC 2 cut(s) 534, 1364
ApeKI GCWGC 3 cut(s) 662, 1238, 1360
ApoI RAATTY 2 cut(s) 376, 437
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 1 cut(s) 1282
AspS9I GGNCC 2 cut(s) 875, 1330
AsuHPI GGTGA 6 cut(s) 188, 281, 328, 618, 704, 1363
AvaI CYCGRG 1 cut(s) 977
AvaII GGWCC 2 cut(s) 875, 1330
AvrII CCTAGG 1 cut(s) 871
Bbv12I GWGCWC 2 cut(s) 802, 865
BbvI GCAGC 3 cut(s) 649, 1225, 1372
BccI CCATC 5 cut(s) 5, 125, 175, 266, 1298
BceAI ACGGC 1 cut(s) 1056
BciT130I CCWGG 4 cut(s) 443, 538, 581, 1334
BclI TGATCA 2 cut(s) 562, 1162
BcoDI GTCTC 1 cut(s) 672
BfaI CTAG 3 cut(s) 648, 872, 957
BfoI RGCGCY 1 cut(s) 1283
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 4 cut(s) 663, 1239, 1361, 1364
BlnI CCTAGG 1 cut(s) 871
BlsI GCNGC 4 cut(s) 664, 1240, 1362, 1365
Bme1390I CCNGG 4 cut(s) 443, 538, 581, 1334
Bme18I GGWCC 2 cut(s) 875, 1330
BmeT110I CYCGRG 1 cut(s) 977
BmgBI CACGTC 1 cut(s) 1288
BmgT120I GGNCC 2 cut(s) 875, 1330
BmrFI CCNGG 4 cut(s) 443, 538, 581, 1334
BmsI GCATC 6 cut(s) 481, 630, 708, 958, 1072, 1188
BoxI GACNNNNGTC 1 cut(s) 873
BpmI CTGGAG 2 cut(s) 559, 879
Bpu10I CCTNAGC 3 cut(s) 216, 261, 801
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 3 cut(s) 442, 871, 976
BsaXI ACNNNNNCTCC 2 cut(s) 1300, 1330
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse1I ACTGG 3 cut(s) 49, 743, 1150
Bse3DI GCAATG 2 cut(s) 757, 822
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 4 cut(s) 443, 538, 581, 1334
BseDI CCNNGG 3 cut(s) 442, 871, 976
BseGI GGATG 5 cut(s) 699, 788, 973, 1264, 1309
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMI GCAATG 2 cut(s) 757, 822
BseMII CTCAG 3 cut(s) 207, 815, 1266
BseNI ACTGG 3 cut(s) 49, 743, 1150
BseXI GCAGC 3 cut(s) 649, 1225, 1372
BsgI GTGCAG 1 cut(s) 1224
BshFI GGCC 2 cut(s) 536, 1366
BsiHKAI GWGCWC 2 cut(s) 802, 865
BsiHKCI CYCGRG 1 cut(s) 977
BslFI GGGAC 1 cut(s) 1051
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 1 cut(s) 672
BsmFI GGGAC 1 cut(s) 1051
BsmI GAATGC 3 cut(s) 244, 400, 850
BsnI GGCC 2 cut(s) 536, 1366
Bso31I GGTCTC 1 cut(s) 672
BsoBI CYCGRG 1 cut(s) 977
Bsp1286I GDGCHC 2 cut(s) 802, 865
Bsp1407I TGTACA 1 cut(s) 1153
BspACI CCGC 2 cut(s) 116, 1363
BspANI GGCC 2 cut(s) 536, 1366
BspCNI CTCAG 3 cut(s) 208, 814, 1267
BspPI GGATC 3 cut(s) 151, 515, 942
BspTNI GGTCTC 1 cut(s) 672
BsrDI GCAATG 2 cut(s) 757, 822
BsrGI TGTACA 1 cut(s) 1153
BsrI ACTGG 3 cut(s) 49, 743, 1150
BssECI CCNNGG 3 cut(s) 442, 871, 976
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 4 cut(s) 443, 538, 581, 1334
Bst4CI ACNGT 3 cut(s) 349, 1135, 1316
BstAPI GCANNNNNTGC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 1153
BstC8I GCNNGC 4 cut(s) 99, 900, 914, 993
BstDEI CTNAG 7 cut(s) 70, 216, 261, 471, 801, 1075, 1275
BstF5I GGATG 5 cut(s) 699, 788, 973, 1264, 1309
BstH2I RGCGCY 1 cut(s) 1283
BstHHI GCGC 1 cut(s) 1282
BstMAI GTCTC 1 cut(s) 672
BstMWI GCNNNNNNNGC 5 cut(s) 113, 491, 542, 1091, 1175
BstNI CCWGG 4 cut(s) 443, 538, 581, 1334
BstNSI RCATGY 4 cut(s) 101, 925, 995, 1057
BstPAI GACNNNNGTC 1 cut(s) 873
BstSCI CCNGG 4 cut(s) 441, 536, 579, 1332
BstV1I GCAGC 3 cut(s) 649, 1225, 1372
BstX2I RGATCY 4 cut(s) 143, 520, 947, 1117
BstYI RGATCY 4 cut(s) 143, 520, 947, 1117
BsuRI GGCC 2 cut(s) 536, 1366
BtrI CACGTC 1 cut(s) 1288
BtsCI GGATG 5 cut(s) 699, 788, 973, 1264, 1309
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 4 cut(s) 114, 666, 1272, 1321
Cac8I GCNNGC 4 cut(s) 99, 900, 914, 993
CfoI GCGC 1 cut(s) 1282
Cfr13I GGNCC 2 cut(s) 875, 1330
Csp6I GTAC 2 cut(s) 15, 1154
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
CviAII CATG 9 cut(s) 98, 234, 704, 827, 840, 922, 992, 1019, 1054
CviQI GTAC 2 cut(s) 15, 1154
DdeI CTNAG 7 cut(s) 70, 216, 261, 471, 801, 1075, 1275
EaeI YGGCCR 1 cut(s) 1364
Eco130I CCWWGG 1 cut(s) 871
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 2 cut(s) 875, 1330
Eco57I CTGAAG 4 cut(s) 123, 924, 1278, 1355
Eco88I CYCGRG 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 4 cut(s) 441, 536, 579, 1332
EcoT14I CCWWGG 1 cut(s) 871
EcoT22I ATGCAT 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 9 cut(s) 101, 237, 707, 830, 843, 925, 995, 1022, 1057
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 1 cut(s) 1051
FatI CATG 9 cut(s) 97, 233, 703, 826, 839, 921, 991, 1018, 1053
FauI CCCGC 1 cut(s) 109
FbaI TGATCA 2 cut(s) 562, 1162
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 4 cut(s) 663, 1239, 1361, 1364
FokI GGATG 5 cut(s) 686, 795, 980, 1271, 1316
Fsp4HI GCNGC 4 cut(s) 663, 1239, 1361, 1364
FspBI CTAG 3 cut(s) 648, 872, 957
GlaI GCGC 1 cut(s) 1281
GluI GCNGC 4 cut(s) 663, 1239, 1361, 1364
GsuI CTGGAG 2 cut(s) 559, 879
HaeII RGCGCY 1 cut(s) 1283
HaeIII GGCC 2 cut(s) 536, 1366
HhaI GCGC 1 cut(s) 1282
Hin1II CATG 9 cut(s) 101, 237, 707, 830, 843, 925, 995, 1022, 1057
Hin6I GCGC 1 cut(s) 1280
HinP1I GCGC 1 cut(s) 1280
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 2 cut(s) 311, 731
HphI GGTGA 6 cut(s) 188, 281, 328, 618, 704, 1363
Hpy166II GTNNAC 2 cut(s) 336, 345
Hpy188I TCNGA 8 cut(s) 130, 288, 316, 393, 736, 834, 1197, 1297
Hpy188III TCNNGA 5 cut(s) 508, 524, 858, 979, 1115
Hpy8I GTNNAC 2 cut(s) 336, 345
HpyAV CCTTC 5 cut(s) 260, 860, 899, 940, 1202
HpyCH4III ACNGT 3 cut(s) 349, 1135, 1316
HpyCH4IV ACGT 1 cut(s) 1287
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 491, 542, 1091, 1175
HpyF3I CTNAG 7 cut(s) 70, 216, 261, 471, 801, 1075, 1275
HpySE526I ACGT 1 cut(s) 1287
Hsp92II CATG 9 cut(s) 101, 237, 707, 830, 843, 925, 995, 1022, 1057
HspAI GCGC 1 cut(s) 1280
Ksp22I TGATCA 2 cut(s) 562, 1162
LmnI GCTCC 1 cut(s) 860
Lsp1109I GCAGC 3 cut(s) 649, 1225, 1372
LweI GCATC 6 cut(s) 481, 630, 708, 958, 1072, 1188
MaeI CTAG 3 cut(s) 648, 872, 957
MaeII ACGT 1 cut(s) 1287
MaeIII GTNAC 2 cut(s) 1288, 1351
MboII GAAGA 2 cut(s) 16, 731
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 4 cut(s) 143, 520, 947, 1117
MhlI GDGCHC 2 cut(s) 802, 865
MluCI AATT 6 cut(s) 290, 376, 437, 486, 635, 777
MmeI TCCRAC 3 cut(s) 121, 371, 1281
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 8 cut(s) 77, 326, 354, 497, 516, 944, 1230, 1322
MslI CAYNNNNRTG 3 cut(s) 690, 1023, 1052
MspA1I CMGCKG 1 cut(s) 1363
MspR9I CCNGG 4 cut(s) 443, 538, 581, 1334
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 3 cut(s) 244, 400, 850
MvaI CCWGG 4 cut(s) 443, 538, 581, 1334
MwoI GCNNNNNNNGC 5 cut(s) 113, 491, 542, 1091, 1175
NlaIII CATG 9 cut(s) 101, 237, 707, 830, 843, 925, 995, 1022, 1057
NmuCI GTSAC 2 cut(s) 1288, 1351
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 4 cut(s) 101, 925, 995, 1057
PaeI GCATGC 2 cut(s) 101, 995
PciI ACATGT 2 cut(s) 921, 1053
PcsI WCGNNNNNNNCGW 1 cut(s) 598
PctI GAATGC 3 cut(s) 244, 400, 850
PfeI GAWTC 2 cut(s) 311, 731
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 4 cut(s) 664, 1240, 1362, 1365
PscI ACATGT 2 cut(s) 921, 1053
PshAI GACNNNNGTC 1 cut(s) 873
Psp6I CCWGG 4 cut(s) 441, 536, 579, 1332
PspGI CCWGG 4 cut(s) 441, 536, 579, 1332
PspPI GGNCC 2 cut(s) 875, 1330
PsuI RGATCY 4 cut(s) 143, 520, 947, 1117
RsaI GTAC 2 cut(s) 16, 1155
RsaNI GTAC 2 cut(s) 15, 1154
RseI CAYNNNNRTG 3 cut(s) 690, 1023, 1052
SaqAI TTAA 8 cut(s) 77, 326, 354, 497, 516, 944, 1230, 1322
SatI GCNGC 4 cut(s) 663, 1239, 1361, 1364
Sau96I GGNCC 2 cut(s) 875, 1330
ScrFI CCNGG 4 cut(s) 443, 538, 581, 1334
SduI GDGCHC 2 cut(s) 802, 865
SfaNI GCATC 6 cut(s) 481, 630, 708, 958, 1072, 1188
SinI GGWCC 2 cut(s) 875, 1330
SmiMI CAYNNNNRTG 3 cut(s) 690, 1023, 1052
SphI GCATGC 2 cut(s) 101, 995
Sse9I AATT 6 cut(s) 290, 376, 437, 486, 635, 777
SsiI CCGC 2 cut(s) 116, 1363
SspMI CTAG 3 cut(s) 648, 872, 957
StyD4I CCNGG 4 cut(s) 441, 536, 579, 1332
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 349, 1135, 1316
TaiI ACGT 1 cut(s) 1290
TaqI TCGA 4 cut(s) 478, 592, 1114, 1269
TasI AATT 6 cut(s) 290, 376, 437, 486, 635, 777
TatI WGTACW 1 cut(s) 1153
TauI GCSGC 1 cut(s) 1366
TfiI GAWTC 2 cut(s) 311, 731
Tru1I TTAA 8 cut(s) 77, 326, 354, 497, 516, 944, 1230, 1322
Tru9I TTAA 8 cut(s) 77, 326, 354, 497, 516, 944, 1230, 1322
TscAI CASTG 4 cut(s) 114, 673, 1279, 1321
TseFI GTSAC 2 cut(s) 1288, 1351
TseI GCWGC 3 cut(s) 662, 1238, 1360
Tsp45I GTSAC 2 cut(s) 1288, 1351
TspDTI ATGAA 7 cut(s) 17, 450, 744, 788, 828, 922, 1275
TspRI CASTG 4 cut(s) 114, 673, 1279, 1321
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 2 cut(s) 875, 1330
XapI RAATTY 2 cut(s) 376, 437
XceI RCATGY 4 cut(s) 101, 925, 995, 1057
XmaJI CCTAGG 1 cut(s) 871
XmiI GTMKAC 1 cut(s) 344
XspI CTAG 3 cut(s) 648, 872, 957
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.