MD15G1204000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012559
Physical Location & Seq
Reverse (-)
16217500 .. 16222721
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1204000.v1.1.491

Sequence Viewer

Length: 2937 bp
ATGTCACCTGCTTTCCGGAGGCTCTTGTTGCCCATTTGCCAAAATCCCTCGCTTCGATTGCATAACAAGCTTAGTCCAGTTTCCAGGTCACTATTGCTCCCTGTTCAATTAAAGCCTTTGTCTTGTGCTGCTTTGGATGTGATTGACTCGAATCCCATCAGTGGGTCGTGTCTCGAATTTGCCCCAAACTCTGAGACTCAGCTTCGTCAAGTAAGATTAGATAATGGCTATTCACCTGATCAGGAAATTGTAGGAAGCAATGTGAAAACCAGGCCTTTAGTTTTCGATACCAAAAAGAGGCTTATGCATTATTCTGGGATGCTTCGTACCTGTGTTTCGCTAAGGTCTTTGAATGAGGGGAAGGCTATTCATGGACAGGTGATAAAAGAAGGGATTGATCCGGATTCGCATTTGTGGGTTTCATTGGTTAATGTTTATGCAAAGTGTGGGGACTCCGGGTATGCACGTAAAGTGCTTGATGTGATGCCGGAAAGAGATGTTGTGTCGTGGACTTCTTTAATTCAGGGGTTTGTAGTCGAAGGTTCCGGTGTTGACGCTGTTAAATTGTTCTGTGAGATGAAGAAAGATGGTACAAAAGCAAATGACTTTGCATTGGCAACTGGGCTGAAAGCATGTTCTTTGTGCTCCGATTTAGGTTTTGGGAAACAGTTGCATGCTGAAGCAGTGAAACAGGGGTTGTTATTGGATGCTTTTGTTGGTTCTGCTCTAGTCGGTCTTTATGCAAAATGTGGTGATATGGAACTTGCAGATAGAGTGTTATTTTGTATGCCTGAGCAGGATGTGGTATCATGGAATGCACTGCTGAATGGGTATGCTCAGGAAGGTGACGGAAAAAAATCTTTGGAATTGTTCTGCAGAATGACAGAATCAGAAATAAGGTTGAGCAAGTCCACCTTGTCCACTGTCCTCAAGGGTTGTGCAAACTCGGGAAATTTGAGCGGAGGCCAGTTTTTGCATTCTCTAGTCGTCAAAATTGGGTTTCAGATAGATGAATTCTTGGGTTGCAGTCTCGTTGATATGTATTCAAAGTGTGGGATGGCAGTCGATGCGGTAAAAGCATTCAGGACGATTGAAAATCCTGATGTAGTGGCCTGGAGTGCAATTATCTCATGCCTTGATCAACAAGGGCAATGGCAAGAAGTGCCTCAGCTATTTCGTGAAATGATAAGTACAGGCATCTCACCAAATAAATTTACCCTTTCTAGTATCATTAGTGCTGCCACTGATCTCGGAGACCTTCATTTTGGTGAAAGTATCCATGCTTTTGCATGGAAATATGGTTGTGAAGCTGATATTTCAGTCAGCAATGCTCTAATCACAATGTATATGAAAAGTGGGCGTTTGCTAGATGGTGCTCAGGTTTTTGAGGCAATGACAAACCCTGATTTGATTTCATGGAATGCCCTTTTATCTGGAGCACACAATCATGAGTTATCTGACCTAGGGCCAAGAGTGTTCCAACAAATGCTTGTGGAAGGTTTGAAGCCCAACATGTACTCATTCATTAGTGTTTTAAGGTGTTGCTCTAGCCTTTTGGATGTGGACCTTGGAAAACAAATACATTCCCATATTATCAAAACTAACCTCGATGATAATGACTTTGTTGGGACAGCTCTCATTGACATGTATGCCAAAGGCAGGTTTGTGGAAGATGCTGTGAAAGCTTTCAATAGATTGAGTAATCGAGACCTCTTCACTTGGACAGTCATCATTACTGGTTATGCCCAAACTGATCAAGCAGAGGAATCTGTTGCTTGCTTCAATAAGATGCAACAAGAAGGAGTGAAGCCAAATGAGTTCAGCATCGCTGGCTGTTTGAGTGCTTGCTCCCGTACAGCTATGCTGGAAAATGGGCGGCAGCTCCACTCAATGGTAATTAAGAGTGGGCATCTGGAAGATTTGTTTGTTACTAGTGCACTTGTTGATATGTATGCAAAATGTGGGTGCATCAGTGATGCTGAGGATATCTTTGAGGGCTTGGTTTCACGAGATACAGTGTCATGGAATATCATGGTGTGTGGATATTCCCAATATGGGCAAGGAGAGAAGGCTCTTGAAGCCTTTTCGACAATGTTGGATGAAGGTGCCATACCAAATGAGATCACCTTCATTGGTGTTCTTGCTGCATGCAGCCACCTGGGTCTAGTCAAAGAAGGGAAAAAGCACTTCGACTCACTGAGCAATGTTTTCGGGATCACTCCTACAATTGAGCATTATGCTTGTATGGTTGATATTCTCGGCAGGGCTGGAAAGTTTAATGAGATTGAAACCTTTATCGAGACAAAGGAACTAACACCGTATGCCATTATTTGGGAGACTGTTCTTGGGGCTTGTAAGATGCATGGAAATGTTGAATTTGGTGAAACAGCAGCAAGAAGGCTTTTTGAGCTTAAACCTGAGATGGATTCAACATATATCTTGCTATCTAATATCTTTGCAGTCAAAGGCAGGTGGGACGATGTTAGCAAAGTCAGGAAATTAATGCAGAGTCAAGGTGTTAAAAAAGAACCTGGTTGTAGCTGGGTTGAGGTTGATGGTCAAGTCAACATTTTTGTCTCTCAAGATGGTACGCATCCAAGAATTGGGGATATTCATTTGAAATTGGAGGAACTAGGAGAAAAGCTAAATTCATTAGGTTATATACCGGAAACAGAAGACGTGCTTCATAGAATCACTGAAAGAGAAAAAAAGGAACATCTCCAATATCACAGTGAAAGGTTGGCTCTTGGTTTTGCCCTTATAAGTACCAGTCATCCAAAAACTATTCGGATCTTTAAAAATCTACGCATCTGTGGAGACTGCCATGACATTATGAAGCTTGTCTCCGATGTCACAAATCGGGAAATAGTTGTTCGTGACATCAGGCGGTTTCATCATTTTAAGAATGGAACTTGCTCCTGTAAGGACTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 map00190 map01100 map04714
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

979

Amino Acids

108.31

Weight (kDa)

5.79

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 165 - 211 2.7e-08 PPR repeat family
PPR PF01535 168 - 197 2.5e-06 PPR repeat
PPR_2 PF13041 267 - 315 3.8e-10 PPR repeat family
PPR PF01535 269 - 297 6.6e-07 PPR repeat
PPR_2 PF13041 367 - 413 9.3e-10 PPR repeat family
PPR_2 PF13041 468 - 515 7.5e-06 PPR repeat family
PPR_2 PF13041 570 - 617 2.2e-08 PPR repeat family
PPR_2 PF13041 670 - 718 3.5e-09 PPR repeat family
E_motif PF20431 788 - 850 3.6e-25 E motif
DYW_deaminase PF14432 886 - 978 8.3e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2762
AarI CACCTGC 2 cut(s) 16, 2460
Acc36I ACCTGC 3 cut(s) 16, 1650, 2460
AccB1I GGYRCC 1 cut(s) 2105
AccB7I CCANNNNNTGG 3 cut(s) 1891, 2331, 2603
AccBSI CCGCTC 1 cut(s) 960
AccIII TCCGGA 2 cut(s) 15, 400
AciI CCGC 4 cut(s) 960, 1070, 1876, 2887
AclWI GGATC 3 cut(s) 392, 2222, 2798
AcsI RAATTY 6 cut(s) 176, 952, 1013, 1211, 2375, 2647
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 7 cut(s) 328, 592, 1192, 1517, 1855, 2590, 2767
AfiI CCNNNNNNNGG 8 cut(s) 161, 162, 297, 1659, 1891, 2055, 2331, 2603
AflIII ACRYGT 2 cut(s) 1512, 1644
AhlI ACTAGT 1 cut(s) 1931
AjiI CACGTC 1 cut(s) 2680
AjnI CCWGG 5 cut(s) 83, 269, 1112, 2157, 2530
Alw21I GWGCWC 4 cut(s) 647, 1378, 1441, 1939
Alw44I GTGCAC 1 cut(s) 1935
AlwI GGATC 3 cut(s) 392, 2222, 2798
Ama87I CYCGRG 1 cut(s) 946
Aor13HI TCCGGA 2 cut(s) 15, 400
AoxI GGCC 4 cut(s) 272, 964, 1110, 1466
ApaLI GTGCAC 1 cut(s) 1935
ApeKI GCWGC 6 cut(s) 128, 1238, 1879, 2144, 2151, 2390
ApoI RAATTY 6 cut(s) 176, 952, 1013, 1211, 2375, 2647
ArsI GACNNNNNNTTYG 2 cut(s) 1247, 1279
AseI ATTAAT 1 cut(s) 2501
Asp700I GAANNNNTTC 1 cut(s) 1685
AspA2I CCTAGG 1 cut(s) 1462
AspS9I GGNCC 2 cut(s) 1466, 1564
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 8 cut(s) 225, 391, 764, 857, 1194, 1280, 2116, 2393
AvaI CYCGRG 1 cut(s) 946
AvaII GGWCC 1 cut(s) 1564
AvrII CCTAGG 1 cut(s) 1462
BaeGI GKGCMC 1 cut(s) 1939
BanI GGYRCC 1 cut(s) 2105
BarI GAAGNNNNNNTAC 2 cut(s) 2094, 2126
BauI CACGAG 1 cut(s) 2007
BbsI GAAGAC 1 cut(s) 2682
Bbv12I GWGCWC 4 cut(s) 647, 1378, 1441, 1939
BbvCI CCTCAGC 2 cut(s) 1167, 1980
BbvI GCAGC 6 cut(s) 115, 1225, 1891, 2131, 2163, 2402
BccI CCATC 7 cut(s) 164, 581, 1051, 1364, 2416, 2549, 2579
BcgI CGANNNNNNTGC 2 cut(s) 2191, 2225
BciT130I CCWGG 5 cut(s) 85, 271, 1114, 2159, 2532
BciVI GTATCC 1 cut(s) 1286
BclI TGATCA 3 cut(s) 238, 1138, 1753
BcnI CCSGG 1 cut(s) 457
BcuI ACTAGT 1 cut(s) 1931
BfaI CTAG 9 cut(s) 728, 983, 1224, 1367, 1463, 1548, 1932, 2165, 2633
BfmI CTRYAG 1 cut(s) 874
BfuAI ACCTGC 3 cut(s) 16, 1650, 2460
BfuI GTATCC 1 cut(s) 1286
BisI GCNGC 7 cut(s) 129, 1239, 1877, 1880, 2145, 2152, 2391
BlnI CCTAGG 1 cut(s) 1462
BlsI GCNGC 7 cut(s) 130, 1240, 1878, 1881, 2146, 2153, 2392
Bme1390I CCNGG 6 cut(s) 85, 271, 457, 1114, 2159, 2532
Bme18I GGWCC 1 cut(s) 1564
BmeT110I CYCGRG 1 cut(s) 946
BmgBI CACGTC 1 cut(s) 2680
BmgT120I GGNCC 2 cut(s) 1466, 1564
BmiI GGNNCC 2 cut(s) 544, 2107
BmrFI CCNGG 6 cut(s) 85, 271, 457, 1114, 2159, 2532
BmrI ACTGGG 1 cut(s) 630
BmuI ACTGGG 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 2682
BpmI CTGGAG 2 cut(s) 1135, 1455
Bpu10I CCTNAGC 6 cut(s) 341, 792, 837, 1167, 1377, 1980
BpuEI CTTGAG 2 cut(s) 914, 2565
BpuMI CCSGG 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 467
BsaI GGTCTC 2 cut(s) 1248, 1701
BsaJI CCNNGG 3 cut(s) 1462, 1567, 2158
BsaWI WCCGGW 4 cut(s) 15, 400, 545, 2665
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 162, 297, 1659, 1891, 2055, 2331, 2603
Bse1I ACTGG 5 cut(s) 77, 625, 967, 1741, 2769
Bse3DI GCAATG 5 cut(s) 265, 1157, 1333, 1398, 2209
BseAI TCCGGA 2 cut(s) 15, 400
BseBI CCWGG 5 cut(s) 85, 271, 1114, 2159, 2532
BseDI CCNNGG 3 cut(s) 1462, 1567, 2158
BseGI GGATG 9 cut(s) 142, 324, 712, 805, 1062, 1564, 2104, 2593, 2773
BseLI CCNNNNNNNGG 8 cut(s) 161, 162, 297, 1659, 1891, 2055, 2331, 2603
BseMI GCAATG 5 cut(s) 265, 1157, 1333, 1398, 2209
BseMII CTCAG 9 cut(s) 183, 212, 783, 851, 1181, 1391, 1971, 2189, 2409
BseNI ACTGG 5 cut(s) 77, 625, 967, 1741, 2769
BseSI GKGCMC 1 cut(s) 1939
BseXI GCAGC 6 cut(s) 115, 1225, 1891, 2131, 2163, 2402
BseYI CCCAGC 1 cut(s) 2541
BshFI GGCC 4 cut(s) 274, 966, 1112, 1468
BshNI GGYRCC 1 cut(s) 2105
BsiHKAI GWGCWC 4 cut(s) 647, 1378, 1441, 1939
BsiHKCI CYCGRG 1 cut(s) 946
BsiSI CCGG 6 cut(s) 16, 401, 456, 488, 546, 2666
BslFI GGGAC 3 cut(s) 464, 1642, 2489
BslI CCNNNNNNNGG 8 cut(s) 161, 162, 297, 1659, 1891, 2055, 2331, 2603
BsmFI GGGAC 3 cut(s) 464, 1642, 2489
BsmI GAATGC 4 cut(s) 820, 976, 1079, 1426
BsnI GGCC 4 cut(s) 274, 966, 1112, 1468
Bso31I GGTCTC 2 cut(s) 1248, 1701
BsoBI CYCGRG 1 cut(s) 946
Bsp1286I GDGCHC 4 cut(s) 647, 1378, 1441, 1939
Bsp13I TCCGGA 2 cut(s) 15, 400
Bsp143I GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
BspACI CCGC 4 cut(s) 960, 1070, 1876, 2887
BspANI GGCC 4 cut(s) 274, 966, 1112, 1468
BspCNI CTCAG 9 cut(s) 184, 211, 784, 850, 1180, 1390, 1972, 2190, 2410
BspEI TCCGGA 2 cut(s) 15, 400
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 2 cut(s) 544, 2107
BspMAI CTGCAG 1 cut(s) 878
BspMI ACCTGC 3 cut(s) 16, 1650, 2460
BspPI GGATC 3 cut(s) 392, 2222, 2798
BspT107I GGYRCC 1 cut(s) 2105
BspTNI GGTCTC 2 cut(s) 1248, 1701
BsrBI CCGCTC 1 cut(s) 960
BsrDI GCAATG 5 cut(s) 265, 1157, 1333, 1398, 2209
BsrI ACTGG 5 cut(s) 77, 625, 967, 1741, 2769
BssECI CCNNGG 3 cut(s) 1462, 1567, 2158
BssMI GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
BssSI CACGAG 1 cut(s) 2007
BssT1I CCWWGG 2 cut(s) 1462, 1567
Bst2BI CACGAG 1 cut(s) 2007
Bst2UI CCWGG 5 cut(s) 85, 271, 1114, 2159, 2532
Bst4CI ACNGT 7 cut(s) 669, 925, 1726, 2017, 2319, 2341, 2732
Bst6I CTCTTC 1 cut(s) 1718
BstAPI GCANNNNNTGC 2 cut(s) 1067, 1162
BstBAI YACGTR 1 cut(s) 467
BstC8I GCNNGC 5 cut(s) 675, 1777, 1831, 1846, 2149
BstF5I GGATG 9 cut(s) 142, 324, 712, 805, 1062, 1564, 2104, 2593, 2773
BstKTI GATC 8 cut(s) 241, 400, 1141, 1249, 1756, 2124, 2217, 2793
BstMBI GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
BstNI CCWGG 5 cut(s) 85, 271, 1114, 2159, 2532
BstNSI RCATGY 5 cut(s) 636, 677, 1516, 1648, 2151
BstSCI CCNGG 6 cut(s) 83, 269, 455, 1112, 2157, 2530
BstSFI CTRYAG 1 cut(s) 874
BstSLI GKGCMC 1 cut(s) 1939
BstV1I GCAGC 6 cut(s) 115, 1225, 1891, 2131, 2163, 2402
BstV2I GAAGAC 1 cut(s) 2682
BstX2I RGATCY 1 cut(s) 2790
BstYI RGATCY 1 cut(s) 2790
BsuI GTATCC 1 cut(s) 1286
BsuRI GGCC 4 cut(s) 274, 966, 1112, 1468
BtgZI GCGATG 1 cut(s) 1810
BtrI CACGTC 1 cut(s) 2680
BtsCI GGATG 9 cut(s) 142, 324, 712, 805, 1062, 1564, 2104, 2593, 2773
BtsI GCAGTG 2 cut(s) 690, 818
BveI ACCTGC 3 cut(s) 16, 1650, 2460
Cac8I GCNNGC 5 cut(s) 675, 1777, 1831, 1846, 2149
CciI TCATGA 1 cut(s) 1447
Cfr13I GGNCC 2 cut(s) 1466, 1564
CseI GACGC 1 cut(s) 563
CsiI ACCWGGT 1 cut(s) 2530
Csp6I GTAC 7 cut(s) 327, 591, 1191, 1516, 1854, 2589, 2766
CviQI GTAC 7 cut(s) 327, 591, 1191, 1516, 1854, 2589, 2766
DpnI GATC 8 cut(s) 240, 399, 1140, 1248, 1755, 2123, 2216, 2792
DpnII GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
DraI TTTAAA 1 cut(s) 2797
Eam1104I CTCTTC 1 cut(s) 1718
EarI CTCTTC 1 cut(s) 1718
Eco130I CCWWGG 2 cut(s) 1462, 1567
Eco147I AGGCCT 1 cut(s) 274
Eco31I GGTCTC 2 cut(s) 1248, 1701
Eco32I GATATC 1 cut(s) 1987
Eco47I GGWCC 1 cut(s) 1564
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 1013
EcoRII CCWGG 5 cut(s) 83, 269, 1112, 2157, 2530
EcoRV GATATC 1 cut(s) 1987
EcoT14I CCWWGG 2 cut(s) 1462, 1567
EcoT22I ATGCAT 2 cut(s) 309, 2364
ErhI CCWWGG 2 cut(s) 1462, 1567
FalI AAGNNNNNCTT 4 cut(s) 899, 931, 2667, 2699
FaqI GGGAC 3 cut(s) 464, 1642, 2489
FbaI TGATCA 3 cut(s) 238, 1138, 1753
Fnu4HI GCNGC 7 cut(s) 129, 1239, 1877, 1880, 2145, 2152, 2391
FokI GGATG 9 cut(s) 149, 331, 719, 812, 1069, 1571, 2111, 2580, 2760
Fsp4HI GCNGC 7 cut(s) 129, 1239, 1877, 1880, 2145, 2152, 2391
FspBI CTAG 9 cut(s) 728, 983, 1224, 1367, 1463, 1548, 1932, 2165, 2633
GluI GCNGC 7 cut(s) 129, 1239, 1877, 1880, 2145, 2152, 2391
GsaI CCCAGC 1 cut(s) 2545
GsuI CTGGAG 2 cut(s) 1135, 1455
HaeIII GGCC 4 cut(s) 274, 966, 1112, 1468
HapII CCGG 6 cut(s) 16, 401, 456, 488, 546, 2666
HgaI GACGC 1 cut(s) 563
HincII GTYRAC 2 cut(s) 553, 2566
HindII GTYRAC 2 cut(s) 553, 2566
HindIII AAGCTT 3 cut(s) 68, 1683, 2837
HpaII CCGG 6 cut(s) 16, 401, 456, 488, 546, 2666
HphI GGTGA 8 cut(s) 225, 391, 764, 857, 1194, 1280, 2116, 2393
Hpy166II GTNNAC 7 cut(s) 510, 553, 912, 921, 1564, 1937, 2566
Hpy188I TCNGA 8 cut(s) 193, 649, 892, 1005, 1253, 1459, 2790, 2848
Hpy8I GTNNAC 7 cut(s) 510, 553, 912, 921, 1564, 1937, 2566
HpyCH4III ACNGT 7 cut(s) 669, 925, 1726, 2017, 2319, 2341, 2732
HpyCH4IV ACGT 2 cut(s) 466, 2679
HpySE526I ACGT 2 cut(s) 466, 2679
Kpn2I TCCGGA 2 cut(s) 15, 400
Ksp22I TGATCA 3 cut(s) 238, 1138, 1753
Kzo9I GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
LmnI GCTCC 6 cut(s) 102, 650, 1436, 1853, 1887, 2921
Lsp1109I GCAGC 6 cut(s) 115, 1225, 1891, 2131, 2163, 2402
MabI ACCWGGT 1 cut(s) 2530
MaeI CTAG 9 cut(s) 728, 983, 1224, 1367, 1463, 1548, 1932, 2165, 2633
MaeII ACGT 2 cut(s) 466, 2679
MaeIII GTNAC 6 cut(s) 3, 87, 845, 1927, 2851, 2876
MalI GATC 8 cut(s) 240, 399, 1140, 1248, 1755, 2123, 2216, 2792
MbiI CCGCTC 1 cut(s) 960
MboI GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
MboII GAAGA 5 cut(s) 592, 1682, 1705, 1928, 2687
MfeI CAATTG 1 cut(s) 2226
MflI RGATCY 1 cut(s) 2790
MhlI GDGCHC 4 cut(s) 647, 1378, 1441, 1939
MlyI GAGTC 5 cut(s) 140, 190, 446, 2186, 2518
MmeI TCCRAC 2 cut(s) 1504, 2076
Mph1103I ATGCAT 2 cut(s) 309, 2364
MroI TCCGGA 2 cut(s) 15, 400
MroXI GAANNNNTTC 1 cut(s) 1685
MslI CAYNNNNRTG 4 cut(s) 1266, 1446, 1643, 2367
MspI CCGG 6 cut(s) 16, 401, 456, 488, 546, 2666
MspR9I CCNGG 6 cut(s) 85, 271, 457, 1114, 2159, 2532
MunI CAATTG 1 cut(s) 2226
Mva1269I GAATGC 4 cut(s) 820, 976, 1079, 1426
MvaI CCWGG 5 cut(s) 85, 271, 1114, 2159, 2532
NciI CCSGG 1 cut(s) 457
NdeII GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
NlaIV GGNNCC 2 cut(s) 544, 2107
NmeAIII GCCGAG 1 cut(s) 2238
NmuCI GTSAC 5 cut(s) 3, 87, 845, 2851, 2876
NsiI ATGCAT 2 cut(s) 309, 2364
NspI RCATGY 5 cut(s) 636, 677, 1516, 1648, 2151
PaeI GCATGC 2 cut(s) 677, 2151
PagI TCATGA 1 cut(s) 1447
PaqCI CACCTGC 2 cut(s) 16, 2460
PceI AGGCCT 1 cut(s) 274
PciI ACATGT 2 cut(s) 1512, 1644
PctI GAATGC 4 cut(s) 820, 976, 1079, 1426
PdmI GAANNNNTTC 1 cut(s) 1685
PfeI GAWTC 6 cut(s) 151, 404, 887, 1766, 2426, 2691
PflMI CCANNNNNTGG 3 cut(s) 1891, 2331, 2603
PkrI GCNGC 7 cut(s) 130, 1240, 1878, 1881, 2146, 2153, 2392
PleI GAGTC 5 cut(s) 140, 190, 446, 2186, 2517
PpsI GAGTC 5 cut(s) 140, 190, 446, 2186, 2517
Ppu21I YACGTR 1 cut(s) 467
PscI ACATGT 2 cut(s) 1512, 1644
PshBI ATTAAT 1 cut(s) 2501
PsiI TTATAA 1 cut(s) 2762
Psp6I CCWGG 5 cut(s) 83, 269, 1112, 2157, 2530
PspFI CCCAGC 1 cut(s) 2541
PspGI CCWGG 5 cut(s) 83, 269, 1112, 2157, 2530
PspN4I GGNNCC 2 cut(s) 544, 2107
PspPI GGNCC 2 cut(s) 1466, 1564
PstI CTGCAG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 2790
RsaI GTAC 7 cut(s) 328, 592, 1192, 1517, 1855, 2590, 2767
RsaNI GTAC 7 cut(s) 327, 591, 1191, 1516, 1854, 2589, 2766
RseI CAYNNNNRTG 4 cut(s) 1266, 1446, 1643, 2367
SatI GCNGC 7 cut(s) 129, 1239, 1877, 1880, 2145, 2152, 2391
Sau3AI GATC 8 cut(s) 238, 397, 1138, 1246, 1753, 2121, 2214, 2790
Sau96I GGNCC 2 cut(s) 1466, 1564
SchI GAGTC 5 cut(s) 140, 190, 446, 2186, 2518
ScrFI CCNGG 6 cut(s) 85, 271, 457, 1114, 2159, 2532
SduI GDGCHC 4 cut(s) 647, 1378, 1441, 1939
SexAI ACCWGGT 1 cut(s) 2530
SfcI CTRYAG 1 cut(s) 874
SinI GGWCC 1 cut(s) 1564
SmiMI CAYNNNNRTG 4 cut(s) 1266, 1446, 1643, 2367
SmlI CTYRAG 2 cut(s) 929, 2580
SmoI CTYRAG 2 cut(s) 929, 2580
SpeI ACTAGT 1 cut(s) 1931
SphI GCATGC 2 cut(s) 677, 2151
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 4 cut(s) 960, 1070, 1876, 2887
SspMI CTAG 9 cut(s) 728, 983, 1224, 1367, 1463, 1548, 1932, 2165, 2633
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 6 cut(s) 83, 269, 455, 1112, 2157, 2530
StyI CCWWGG 2 cut(s) 1462, 1567
TaaI ACNGT 7 cut(s) 669, 925, 1726, 2017, 2319, 2341, 2732
TaiI ACGT 2 cut(s) 469, 2682
TaqII GACCGA 1 cut(s) 722
TatI WGTACW 2 cut(s) 1190, 1515
TauI GCSGC 1 cut(s) 1879
TfiI GAWTC 6 cut(s) 151, 404, 887, 1766, 2426, 2691
TseFI GTSAC 5 cut(s) 3, 87, 845, 2851, 2876
TseI GCWGC 6 cut(s) 128, 1238, 1879, 2144, 2151, 2390
Tsp45I GTSAC 5 cut(s) 3, 87, 845, 2851, 2876
TspGWI ACGGA 1 cut(s) 864
Van91I CCANNNNNTGG 3 cut(s) 1891, 2331, 2603
VneI GTGCAC 1 cut(s) 1935
VpaK11BI GGWCC 1 cut(s) 1564
VspI ATTAAT 1 cut(s) 2501
XapI RAATTY 6 cut(s) 176, 952, 1013, 1211, 2375, 2647
XceI RCATGY 5 cut(s) 636, 677, 1516, 1648, 2151
XmaJI CCTAGG 1 cut(s) 1462
XmnI GAANNNNTTC 1 cut(s) 1685
XspI CTAG 9 cut(s) 728, 983, 1224, 1367, 1463, 1548, 1932, 2165, 2633
Zsp2I ATGCAT 2 cut(s) 309, 2364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.