Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007577

Physical Location & Seq

Genomic Coordinates Forward (+)

6451169 .. 6455154

Gene Structure 3986 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1082400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGGAACCTCGCGTTGGGAATAAGTTTCGGCTCGGCCGGAAGATCGGCAGCGGCTCGTTCGGAGAGATCTATCTGGGTACAAATATTCAGACGAACGAAGAGGTCGCGATTAAGCTTGAAAATGTGAAGACAAAACACCCTCAGCTGCTGTACGAATCCAAGTTATACAGGATCCTAGCAGGAGGAACTGGGATTCCAAATGTGAGGTGGTTTGGAGTTGAAGGAGACTATAACGTTTTGGTTATGGATTTACTGGGCCCTAGTCTTGAAGACTTATTTAACTTCTGCAGTAGGAAACTTTCTTTGAAGACCGTTCTTATGCTGGCTGATCAAATGATCAGTCGTGTTGAATTTGTTCACAGTAAGTCATTTCTACATCGAGATATCAAGCCAGACAATTTTCTTATGGGCTTGGGAAGGCGTGCAAATCAGGTGTACATGATAGACTTTGGTCTGGCTAAGAAATACAGAGATAGTACAACCCGTCAGCATATTCCTTATAGGGAAAACAAGAATTTGACTGGAACTGCAAGATATGCAAGCATGAACACTCACCTTGGAATTGAGCAAAGCCGGAGGGATGATTTAGAATCTCTTGGTTATGTCCTTATGTATTTCCTTAGAGGAAGTCTTCCTTGGCAGGGACTAAAAGCGGGAACTAAGAAGCAGAAGTATGAGAGAATTAGTGAAAAAAAGGTTTCTACTTCAATTGAGGCGTTATGTCGGGGTTATCCGACAGAGTTTGCTTCGTATTTCCATTACTGTCGCTCTCTACGGTTCGATGATAAGCCAGACTATGTTTATTTAAAAAAAATATTCCGGGATGTCTTTATTCGTGAAGGCTTTCAGTTTGATTATGTGTTTGACTGGACAATTTTGAAGTATCAGCAATCACAGCTGGCTGTTCCTCCAACCCGTGCCCTTGCTGGTGCTGGAACAAGTTCTGGGATGCCCCATGCTGCTGTTAATGCGGACAGACTGACAGGTGAGGTAGACGGGCGGCCAACTGGTCTAACATCATTGGATTCCTCTCGGCGGAGAATACCTGGACCGGCTCTCAATTCCATGAGCCTTTCGAGGCAGAAGAATCCAATTGCGAATGATGCTTCATTAACCAGAGAGACGTCAAATAGCAACATGTTTGGCCGGTCAGCCGGATCATCAAGGAGAGTTGCTGTTTCTAGCAGCCGTGATGCATTTGGCGGAAGTGAGTCTGACATTCGCCCTCGAACAACCGATGCTAGCCCTGGAGGACACAGAATTTCAAGTGGCCGAAGAAGTTCACCAGTCGAGTCATCGGACCCCACCCGGAGACATACATCTCAGACTAGGAACTACGAGACTACCCTTAAAGGCATCGAAGGTCTGCATTTAGACAATGAAGAGAGGGTTCATTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0008150 GO:0008152 GO:0008360 GO:0009506 GO:0009987 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0030054 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051179 GO:0051234 GO:0055044 GO:0065007 GO:0065008 GO:0071704 GO:0098657 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04011 ko04392 map04011 map04392

Pfam Domains

Protein Families

Pkinase

Protein Analysis

467

Amino Acids

52.62

Weight (kDa)

9.71

Isoelectric Point (pI)

47.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	10 - 224	9.2e-26	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	10 - 231	5.9e-15	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	108 - 206	1.2e-08	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26100 AT5G57015
fragaria_vesca	FvH4_1g07860 FvH4_1g07860
malus_domestica	MD02G1082400.v1.1 MD15G1210300.v1.1
prunus_persica	Prupe.3G046000_v2.0.a1 Prupe.7G206400_v2.0.a1
pyrus_communis	pycom02g06530 pycom15g18640
rosa_chinensis	RchiOBHm_Chr2g0093731
rosa_laevigata	RLG00000016433 RLG00000034433
rosa_multiflora	Rmu_co8213396.1_g000001 Rmu_co8410961.1_g000001 Rmu_sc0002126.1_g000006
rosa_roxburghii	Rroxscaffold_2G00147840
rosa_rugosa	Rorug02G0039400
rosa_samantha	Rh2AG086000 Rh2BG086100 Rh2CG088500 Rh2DG084100 Rh2DG455700 Rh5DG001800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1127
AccI	GTMKAC	1 cut(s)	993
AccII	CGCG	2 cut(s)	12, 107
AciI	CCGC	6 cut(s)	51, 653, 971, 1000, 1036, 1203
AclI	AACGTT	1 cut(s)	234
AclWI	GGATC	3 cut(s)	166, 179, 1165
AcoI	YGGCCR	4 cut(s)	34, 1001, 1144, 1270
AcsI	RAATTY	3 cut(s)	350, 514, 1260
AcyI	GRCGYC	1 cut(s)	1124
AfaI	GTAC	4 cut(s)	79, 152, 437, 478
AfiI	CCNNNNNNNGG	3 cut(s)	14, 641, 1035
AflIII	ACRYGT	1 cut(s)	1137
AgsI	TTSAA	8 cut(s)	119, 221, 269, 307, 350, 708, 880, 1266
AjnI	CCWGG	2 cut(s)	1045, 1246
AjuI	GAANNNNNNNTTGG	3 cut(s)	29, 619, 651
AloI	GAACNNNNNNTCC	2 cut(s)	178, 210
AluBI	AGCT	3 cut(s)	115, 145, 898
AluI	AGCT	3 cut(s)	115, 145, 898
Alw26I	GTCTC	4 cut(s)	219, 1115, 1306, 1334
AlwI	GGATC	3 cut(s)	166, 179, 1165
AlwNI	CAGNNNCTG	1 cut(s)	148
AoxI	GGCC	5 cut(s)	34, 256, 1001, 1144, 1270
ApaI	GGGCCC	1 cut(s)	260
ApeKI	GCWGC	4 cut(s)	48, 145, 959, 1185
ApoI	RAATTY	3 cut(s)	350, 514, 1260
ArsI	GACNNNNNNTTYG	2 cut(s)	325, 357
Asp700I	GAANNNNTTC	4 cut(s)	354, 843, 940, 1279
AspS9I	GGNCC	4 cut(s)	256, 257, 1049, 1300
AsuC2I	CCSGG	2 cut(s)	821, 1309
AsuHPI	GGTGA	3 cut(s)	545, 998, 1275
AsuNHI	GCTAGC	1 cut(s)	1241
AvaII	GGWCC	2 cut(s)	1049, 1300
BaeGI	GKGCMC	2 cut(s)	260, 922
BamHI	GGATCC	1 cut(s)	171
BanII	GRGCYC	1 cut(s)	260
BbsI	GAAGAC	4 cut(s)	134, 276, 314, 623
BbvCI	CCTCAGC	1 cut(s)	141
BbvI	GCAGC	4 cut(s)	60, 132, 946, 1197
BceAI	ACGGC	1 cut(s)	1173
BciT130I	CCWGG	2 cut(s)	1047, 1248
BclI	TGATCA	2 cut(s)	328, 336
BcnI	CCSGG	2 cut(s)	821, 1309
BcoDI	GTCTC	4 cut(s)	219, 1115, 1306, 1334
BfaI	CTAG	5 cut(s)	176, 261, 1182, 1242, 1329
BfmI	CTRYAG	1 cut(s)	286
BglII	AGATCT	1 cut(s)	66
BisI	GCNGC	6 cut(s)	49, 52, 146, 960, 1001, 1186
BlsI	GCNGC	6 cut(s)	50, 53, 147, 961, 1002, 1187
Bme1390I	CCNGG	4 cut(s)	821, 1047, 1248, 1309
Bme18I	GGWCC	2 cut(s)	1049, 1300
BmgT120I	GGNCC	4 cut(s)	256, 257, 1049, 1300
BmiI	GGNNCC	4 cut(s)	6, 173, 258, 1302
BmrFI	CCNGG	4 cut(s)	821, 1047, 1248, 1309
BmrI	ACTGGG	2 cut(s)	198, 263
BmsI	GCATC	5 cut(s)	939, 1093, 1183, 1228, 1365
BmtI	GCTAGC	1 cut(s)	1245
BmuI	ACTGGG	2 cut(s)	198, 263
BoxI	GACNNNNGTC	1 cut(s)	450
BpiI	GAAGAC	4 cut(s)	134, 276, 314, 623
BpmI	CTGGAG	1 cut(s)	1269
Bpu10I	CCTNAGC	1 cut(s)	141
BpuMI	CCSGG	2 cut(s)	821, 1309
BsaHI	GRCGYC	1 cut(s)	1124
BsaJI	CCNNGG	3 cut(s)	556, 635, 1246
Bsc4I	CCNNNNNNNGG	3 cut(s)	14, 641, 1035
Bse118I	RCCGGY	2 cut(s)	1051, 1146
Bse1I	ACTGG	6 cut(s)	193, 258, 526, 872, 1012, 1286
BseBI	CCWGG	2 cut(s)	1047, 1248
BseDI	CCNNGG	3 cut(s)	556, 635, 1246
BseGI	GGATG	3 cut(s)	586, 829, 954
BseLI	CCNNNNNNNGG	3 cut(s)	14, 641, 1035
BseMII	CTCAG	2 cut(s)	155, 1337
BseNI	ACTGG	6 cut(s)	193, 258, 526, 872, 1012, 1286
BseSI	GKGCMC	2 cut(s)	260, 922
BseX3I	CGGCCG	1 cut(s)	34
BseXI	GCAGC	4 cut(s)	60, 132, 946, 1197
Bsh1236I	CGCG	2 cut(s)	12, 107
Bsh1285I	CGRYCG	1 cut(s)	37
BshFI	GGCC	5 cut(s)	36, 258, 1003, 1146, 1272
BsiEI	CGRYCG	1 cut(s)	37
BsiSI	CCGG	7 cut(s)	37, 574, 820, 1052, 1147, 1155, 1309
BslFI	GGGAC	1 cut(s)	657
BslI	CCNNNNNNNGG	3 cut(s)	14, 641, 1035
BsmAI	GTCTC	4 cut(s)	219, 1115, 1306, 1334
BsmBI	CGTCTC	1 cut(s)	1115
BsmFI	GGGAC	1 cut(s)	657
BsnI	GGCC	5 cut(s)	36, 258, 1003, 1146, 1272
Bsp120I	GGGCCC	1 cut(s)	256
Bsp1286I	GDGCHC	2 cut(s)	260, 922
Bsp1407I	TGTACA	1 cut(s)	435
Bsp143I	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
Bsp68I	TCGCGA	1 cut(s)	107
BspACI	CCGC	6 cut(s)	51, 653, 971, 1000, 1036, 1203
BspANI	GGCC	5 cut(s)	36, 258, 1003, 1146, 1272
BspCNI	CTCAG	2 cut(s)	154, 1336
BspFNI	CGCG	2 cut(s)	12, 107
BspLI	GGNNCC	4 cut(s)	6, 173, 258, 1302
BspMAI	CTGCAG	1 cut(s)	290
BspOI	GCTAGC	1 cut(s)	1245
BspPI	GGATC	3 cut(s)	166, 179, 1165
BsrFI	RCCGGY	2 cut(s)	1051, 1146
BsrGI	TGTACA	1 cut(s)	435
BsrI	ACTGG	6 cut(s)	193, 258, 526, 872, 1012, 1286
BssAI	RCCGGY	2 cut(s)	1051, 1146
BssECI	CCNNGG	3 cut(s)	556, 635, 1246
BssMI	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
BssNI	GRCGYC	1 cut(s)	1124
BssT1I	CCWWGG	2 cut(s)	556, 635
Bst2UI	CCWGG	2 cut(s)	1047, 1248
Bst4CI	ACNGT	4 cut(s)	313, 362, 764, 777
Bst6I	CTCTTC	2 cut(s)	93, 1377
BstACI	GRCGYC	1 cut(s)	1124
BstAPI	GCANNNNNTGC	1 cut(s)	536
BstAUI	TGTACA	1 cut(s)	435
BstC8I	GCNNGC	5 cut(s)	324, 423, 541, 900, 1243
BstDEI	CTNAG	5 cut(s)	141, 459, 620, 660, 1323
BstF5I	GGATG	3 cut(s)	586, 829, 954
BstFNI	CGCG	2 cut(s)	12, 107
BstKTI	GATC	6 cut(s)	45, 69, 174, 331, 339, 1160
BstMAI	GTCTC	4 cut(s)	219, 1115, 1306, 1334
BstMBI	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
BstMCI	CGRYCG	1 cut(s)	37
BstMWI	GCNNNNNNNGC	4 cut(s)	536, 895, 968, 1103
BstNI	CCWGG	2 cut(s)	1047, 1248
BstNSI	RCATGY	1 cut(s)	1141
BstPAI	GACNNNNGTC	1 cut(s)	450
BstSCI	CCNGG	4 cut(s)	819, 1045, 1246, 1307
BstSFI	CTRYAG	1 cut(s)	286
BstSLI	GKGCMC	2 cut(s)	260, 922
BstUI	CGCG	2 cut(s)	12, 107
BstV1I	GCAGC	4 cut(s)	60, 132, 946, 1197
BstV2I	GAAGAC	4 cut(s)	134, 276, 314, 623
BstX2I	RGATCY	2 cut(s)	66, 171
BstYI	RGATCY	2 cut(s)	66, 171
BstZI	CGGCCG	1 cut(s)	34
BsuRI	GGCC	5 cut(s)	36, 258, 1003, 1146, 1272
BtsCI	GGATG	3 cut(s)	586, 829, 954
BtuMI	TCGCGA	1 cut(s)	107
Cac8I	GCNNGC	5 cut(s)	324, 423, 541, 900, 1243
CaiI	CAGNNNCTG	1 cut(s)	148
Cfr10I	RCCGGY	2 cut(s)	1051, 1146
Cfr13I	GGNCC	4 cut(s)	256, 257, 1049, 1300
Csp6I	GTAC	4 cut(s)	78, 151, 436, 477
CviAII	CATG	5 cut(s)	439, 544, 956, 1066, 1138
CviQI	GTAC	4 cut(s)	78, 151, 436, 477
DdeI	CTNAG	5 cut(s)	141, 459, 620, 660, 1323
DpnI	GATC	6 cut(s)	44, 68, 173, 330, 338, 1159
DpnII	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
DraI	TTTAAA	1 cut(s)	807
EaeI	YGGCCR	4 cut(s)	34, 1001, 1144, 1270
EagI	CGGCCG	1 cut(s)	34
Eam1104I	CTCTTC	2 cut(s)	93, 1377
EarI	CTCTTC	2 cut(s)	93, 1377
EciI	GGCGGA	2 cut(s)	1051, 1218
EclXI	CGGCCG	1 cut(s)	34
Eco130I	CCWWGG	2 cut(s)	556, 635
Eco24I	GRGCYC	1 cut(s)	260
Eco32I	GATATC	1 cut(s)	385
Eco47I	GGWCC	2 cut(s)	1049, 1300
Eco52I	CGGCCG	1 cut(s)	34
EcoO109I	RGGNCCY	1 cut(s)	257
EcoRII	CCWGG	2 cut(s)	1045, 1246
EcoRV	GATATC	1 cut(s)	385
EcoT14I	CCWWGG	2 cut(s)	556, 635
EcoT22I	ATGCAT	1 cut(s)	1198
EcoT38I	GRGCYC	1 cut(s)	260
ErhI	CCWWGG	2 cut(s)	556, 635
Esp3I	CGTCTC	1 cut(s)	1115
FaeI	CATG	5 cut(s)	442, 547, 959, 1069, 1141
FalI	AAGNNNNNCTT	2 cut(s)	619, 651
FaqI	GGGAC	1 cut(s)	657
FatI	CATG	5 cut(s)	438, 543, 955, 1065, 1137
FauI	CCCGC	1 cut(s)	646
FbaI	TGATCA	2 cut(s)	328, 336
FblI	GTMKAC	1 cut(s)	993
Fnu4HI	GCNGC	6 cut(s)	49, 52, 146, 960, 1001, 1186
FokI	GGATG	3 cut(s)	593, 836, 961
FriOI	GRGCYC	1 cut(s)	260
Fsp4HI	GCNGC	6 cut(s)	49, 52, 146, 960, 1001, 1186
FspBI	CTAG	5 cut(s)	176, 261, 1182, 1242, 1329
GluI	GCNGC	6 cut(s)	49, 52, 146, 960, 1001, 1186
GsuI	CTGGAG	1 cut(s)	1269
HaeIII	GGCC	5 cut(s)	36, 258, 1003, 1146, 1272
HapII	CCGG	7 cut(s)	37, 574, 820, 1052, 1147, 1155, 1309
Hin1I	GRCGYC	1 cut(s)	1124
Hin1II	CATG	5 cut(s)	442, 547, 959, 1069, 1141
HindIII	AAGCTT	1 cut(s)	113
HinfI	GANTC	7 cut(s)	155, 193, 590, 1025, 1087, 1211, 1292
HpaII	CCGG	7 cut(s)	37, 574, 820, 1052, 1147, 1155, 1309
HphI	GGTGA	3 cut(s)	545, 998, 1275
Hpy166II	GTNNAC	4 cut(s)	358, 436, 994, 1283
Hpy188I	TCNGA	6 cut(s)	62, 90, 735, 1216, 1300, 1326
Hpy188III	TCNNGA	4 cut(s)	106, 266, 380, 836
Hpy8I	GTNNAC	4 cut(s)	358, 436, 994, 1283
HpyAV	CCTTC	4 cut(s)	215, 411, 833, 1355
HpyCH4III	ACNGT	4 cut(s)	313, 362, 764, 777
HpyCH4IV	ACGT	2 cut(s)	234, 1124
HpyCH4V	TGCA	6 cut(s)	288, 425, 530, 539, 1196, 1369
HpyF10VI	GCNNNNNNNGC	4 cut(s)	536, 895, 968, 1103
HpyF3I	CTNAG	5 cut(s)	141, 459, 620, 660, 1323
HpySE526I	ACGT	2 cut(s)	234, 1124
Hsp92I	GRCGYC	1 cut(s)	1124
Hsp92II	CATG	5 cut(s)	442, 547, 959, 1069, 1141
Ksp22I	TGATCA	2 cut(s)	328, 336
Kzo9I	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
Lsp1109I	GCAGC	4 cut(s)	60, 132, 946, 1197
LweI	GCATC	5 cut(s)	939, 1093, 1183, 1228, 1365
MaeI	CTAG	5 cut(s)	176, 261, 1182, 1242, 1329
MaeII	ACGT	2 cut(s)	234, 1124
MalI	GATC	6 cut(s)	44, 68, 173, 330, 338, 1159
MboI	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
MboII	GAAGA	9 cut(s)	52, 110, 139, 281, 319, 623, 1096, 1287, 1394
MfeI	CAATTG	2 cut(s)	708, 1092
MflI	RGATCY	2 cut(s)	66, 171
MhlI	GDGCHC	2 cut(s)	260, 922
MlyI	GAGTC	2 cut(s)	1220, 1301
MmeI	TCCRAC	2 cut(s)	758, 935
Mph1103I	ATGCAT	1 cut(s)	1198
MroXI	GAANNNNTTC	4 cut(s)	354, 843, 940, 1279
MseI	TTAA	6 cut(s)	111, 279, 806, 966, 1112, 1350
MspA1I	CMGCKG	3 cut(s)	51, 145, 898
MspI	CCGG	7 cut(s)	37, 574, 820, 1052, 1147, 1155, 1309
MspR9I	CCNGG	4 cut(s)	821, 1047, 1248, 1309
MunI	CAATTG	2 cut(s)	708, 1092
MvaI	CCWGG	2 cut(s)	1047, 1248
MvnI	CGCG	2 cut(s)	12, 107
MwoI	GCNNNNNNNGC	4 cut(s)	536, 895, 968, 1103
NciI	CCSGG	2 cut(s)	821, 1309
NdeII	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
NheI	GCTAGC	1 cut(s)	1241
NlaIII	CATG	5 cut(s)	442, 547, 959, 1069, 1141
NlaIV	GGNNCC	4 cut(s)	6, 173, 258, 1302
NmeAIII	GCCGAG	2 cut(s)	12, 1012
NruI	TCGCGA	1 cut(s)	107
NsiI	ATGCAT	1 cut(s)	1198
NspI	RCATGY	1 cut(s)	1141
PciI	ACATGT	1 cut(s)	1137
PdmI	GAANNNNTTC	4 cut(s)	354, 843, 940, 1279
PfeI	GAWTC	5 cut(s)	155, 193, 590, 1025, 1087
PfoI	TCCNGGA	1 cut(s)	819
PkrI	GCNGC	6 cut(s)	50, 53, 147, 961, 1002, 1187
PleI	GAGTC	2 cut(s)	1219, 1300
PpsI	GAGTC	2 cut(s)	1219, 1300
PscI	ACATGT	1 cut(s)	1137
PshAI	GACNNNNGTC	1 cut(s)	450
Psp1406I	AACGTT	1 cut(s)	234
Psp6I	CCWGG	2 cut(s)	1045, 1246
PspGI	CCWGG	2 cut(s)	1045, 1246
PspN4I	GGNNCC	4 cut(s)	6, 173, 258, 1302
PspOMI	GGGCCC	1 cut(s)	256
PspPI	GGNCC	4 cut(s)	256, 257, 1049, 1300
PstI	CTGCAG	1 cut(s)	290
PstNI	CAGNNNCTG	1 cut(s)	148
PsuI	RGATCY	2 cut(s)	66, 171
PvuII	CAGCTG	2 cut(s)	145, 898
RruI	TCGCGA	1 cut(s)	107
RsaI	GTAC	4 cut(s)	79, 152, 437, 478
RsaNI	GTAC	4 cut(s)	78, 151, 436, 477
SaqAI	TTAA	6 cut(s)	111, 279, 806, 966, 1112, 1350
SatI	GCNGC	6 cut(s)	49, 52, 146, 960, 1001, 1186
Sau3AI	GATC	6 cut(s)	42, 66, 171, 328, 336, 1157
Sau96I	GGNCC	4 cut(s)	256, 257, 1049, 1300
SchI	GAGTC	2 cut(s)	1220, 1301
ScrFI	CCNGG	4 cut(s)	821, 1047, 1248, 1309
SduI	GDGCHC	2 cut(s)	260, 922
SfaNI	GCATC	5 cut(s)	939, 1093, 1183, 1228, 1365
SfcI	CTRYAG	1 cut(s)	286
SinI	GGWCC	2 cut(s)	1049, 1300
SsiI	CCGC	6 cut(s)	51, 653, 971, 1000, 1036, 1203
SspI	AATATT	2 cut(s)	85, 816
SspMI	CTAG	5 cut(s)	176, 261, 1182, 1242, 1329
StyD4I	CCNGG	4 cut(s)	819, 1045, 1246, 1307
StyI	CCWWGG	2 cut(s)	556, 635
TaaI	ACNGT	4 cut(s)	313, 362, 764, 777
TaiI	ACGT	2 cut(s)	237, 1127
TaqI	TCGA	6 cut(s)	379, 780, 1076, 1228, 1290, 1359
TatI	WGTACW	2 cut(s)	435, 476
TauI	GCSGC	2 cut(s)	54, 1003
TfiI	GAWTC	5 cut(s)	155, 193, 590, 1025, 1087
Tru1I	TTAA	6 cut(s)	111, 279, 806, 966, 1112, 1350
Tru9I	TTAA	6 cut(s)	111, 279, 806, 966, 1112, 1350
TseI	GCWGC	4 cut(s)	48, 145, 959, 1185
TspDTI	ATGAA	4 cut(s)	560, 1098, 1382, 1395
VpaK11BI	GGWCC	2 cut(s)	1049, 1300
XapI	RAATTY	3 cut(s)	350, 514, 1260
XceI	RCATGY	1 cut(s)	1141
XcmI	CCANNNNNNNNNTGG	1 cut(s)	204
XmiI	GTMKAC	1 cut(s)	993
XmnI	GAANNNNTTC	4 cut(s)	354, 843, 940, 1279
XspI	CTAG	5 cut(s)	176, 261, 1182, 1242, 1329
ZraI	GACGTC	1 cut(s)	1125
Zsp2I	ATGCAT	1 cut(s)	1198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.