MD02G1103800.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012560
Physical Location & Seq
Forward (+)
8294287 .. 8296577
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1103800.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGACAAGTACATTAATCAAATTTTCAGTTCCTCATCGTGGTCAGATGTCGATGCGAAGGAAATACCATCTTGGGCATGTTGTGAATCTGAGCAACCGAATGCAGCGCTTCTTGGTTCAGTAGTTGCCGTGGATGAACATGAGAACAATAGTGCACCTACATATCTGACTAATTCGAACAGTTTGGAGAGCTTGGTTGCTCAAGATGTTTCATCTATTCTTCAAGATTTTGCACCAGATGCACAAGATGGTGGAAAAAATTGCCGGGTTCAAAATATTGGCATGCACTATGGTGTGGATGGGAACATGGTGAGAATACCTCTTCGCTCCATTGCACCGGAAAATGCGGGATGCGATAGCTTCGAACCACCCCTCTACCAGGGATTCAACGGAGATTTTGAAACCCTTTCTCCAATTTCTCAACCGTGGCCTCTTCAATCTTATGAGGGTGTTTCTCCTGCTCCCGCTTCTGGCATGGGACAACGCAAAATGTGCAGCTTTGGAATAAATGGAGATTATGGGGATTATGTCATGCCAAGCGATCCCAACAACCTGGCTGTTTTTGTCTCCACAGAGAAGGGGATGCAGGACGTACAAAATTATCCTTTGCCTTCCTTCGCTTCTGCTCCTCGGATGTCTGGTTTGCAGTCTTTACCACAGAATACGTCAACTAACCCTGTTGGTGAATGTAATGGAAATAGAAAGCTTCGAATGAGGGCACGTCGAGGCCAGGCTACTGATCCACACAGTATTGCTGAAAGGGTTACTTGTGCACTTCGGAGGGAAAAAATTTCTGATAGGATGAAGAATCTGCAAGAACTAGTGCCAAAGTCTAACAGGACCGACAAGGCATCTATGCTTGATGAAATCATTGAGTATGTCAGATTTCTTCAGCTCCAAGTCAAGGAACTAAGCATGTGCAAAGTTGGGGCAGCTGGGGCAGTTGTCCCTCTCACCACAGACAGTCAAGCTAAGGTCGGGAATGGTTTGCCGCAGTTTAGGACAGCTGGTGACGTTTCTTTTGACGAAATTGCTTTAGAACAGCTGGTGAGGCTGATGGAATCAGATGCGGACAAGGCAATGCAGCATCTCCAAAGCAAAGGTTTCTGCCTCATGCCGGTTGCTCTTGCTGATGCCATTTCTGCAGCAAAGGAATCATCATCGTCGTGGCCGTCAGCTCCCGTTCCTGATGATTGGAAGAAGAAGGAAACCTGTTTCACTGATATCGGCGTTGTTCAGAATAACAGCCGCAGTAGCAGTAACAGCAGCAGCTCACCTGATCAGATAAAACGAGAAGTCAACAACAAATTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

439

Amino Acids

47.72

Weight (kDa)

4.95

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 260 - 298 1.3e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 347, 465, 992, 1070, 1249
AclWI GGATC 2 cut(s) 536, 734
AcoI YGGCCR 1 cut(s) 1169
AcsI RAATTY 2 cut(s) 21, 789
AcuI CTGAAG 1 cut(s) 875
AfaI GTAC 2 cut(s) 11, 594
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 5 cut(s) 39, 379, 470, 904, 1117
AgsI TTSAA 5 cut(s) 224, 272, 388, 401, 437
AhlI ACTAGT 1 cut(s) 820
AjiI CACGTC 1 cut(s) 722
AjnI CCWGG 3 cut(s) 378, 552, 729
AleI CACNNNNGTG 1 cut(s) 291
Alw21I GWGCWC 2 cut(s) 157, 775
Alw26I GTCTC 1 cut(s) 571
Alw44I GTGCAC 2 cut(s) 153, 771
AlwI GGATC 2 cut(s) 536, 734
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 3 cut(s) 428, 727, 1169
ApaLI GTGCAC 2 cut(s) 153, 771
ApeKI GCWGC 7 cut(s) 104, 495, 932, 1084, 1145, 1266, 1269
ApoI RAATTY 2 cut(s) 21, 789
ArsI GACNNNNNNTTYG 2 cut(s) 1004, 1036
AseI ATTAAT 1 cut(s) 15
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 840
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 6 cut(s) 322, 695, 946, 1022, 1060, 1266
AsuII TTCGAA 3 cut(s) 176, 363, 709
AvaII GGWCC 1 cut(s) 840
BaeGI GKGCMC 3 cut(s) 157, 721, 775
Bbv12I GWGCWC 2 cut(s) 157, 775
BbvI GCAGC 7 cut(s) 116, 507, 944, 1096, 1157, 1278, 1281
BccI CCATC 4 cut(s) 76, 242, 293, 1051
BceAI ACGGC 2 cut(s) 113, 1156
BciT130I CCWGG 3 cut(s) 380, 554, 731
BclI TGATCA 1 cut(s) 1279
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 571
BcuI ACTAGT 1 cut(s) 820
BfaI CTAG 1 cut(s) 821
BfmI CTRYAG 1 cut(s) 1143
BfoI RGCGCY 1 cut(s) 110
BisI GCNGC 9 cut(s) 105, 496, 933, 992, 1085, 1146, 1249, 1267, 1270
BlsI GCNGC 9 cut(s) 106, 497, 934, 993, 1086, 1147, 1250, 1268, 1271
Bme1390I CCNGG 4 cut(s) 266, 380, 554, 731
Bme18I GGWCC 1 cut(s) 840
BmgBI CACGTC 1 cut(s) 722
BmgT120I GGNCC 1 cut(s) 840
BmrFI CCNGG 4 cut(s) 266, 380, 554, 731
BmsI GCATC 8 cut(s) 43, 229, 341, 573, 860, 1057, 1096, 1123
BplI GAGNNNNNCTC 2 cut(s) 304, 336
Bpu10I CCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 3 cut(s) 176, 363, 709
BpuEI CTTGAG 1 cut(s) 186
BpuMI CCSGG 1 cut(s) 266
BsaJI CCNNGG 4 cut(s) 129, 379, 425, 629
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 394, 424
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 379, 470, 904, 1117
Bse118I RCCGGY 1 cut(s) 1117
Bse3DI GCAATG 2 cut(s) 330, 1086
BseBI CCWGG 3 cut(s) 380, 554, 731
BseDI CCNNGG 4 cut(s) 129, 379, 425, 629
BseGI GGATG 6 cut(s) 139, 304, 356, 588, 639, 807
BseLI CCNNNNNNNGG 5 cut(s) 39, 379, 470, 904, 1117
BseMI GCAATG 2 cut(s) 330, 1086
BseMII CTCAG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 618
BseSI GKGCMC 3 cut(s) 157, 721, 775
BseXI GCAGC 7 cut(s) 116, 507, 944, 1096, 1157, 1278, 1281
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 514
BshFI GGCC 3 cut(s) 430, 729, 1171
BsiHKAI GWGCWC 2 cut(s) 157, 775
BsiSI CCGG 3 cut(s) 265, 338, 1118
BslFI GGGAC 2 cut(s) 492, 932
BslI CCNNNNNNNGG 5 cut(s) 39, 379, 470, 904, 1117
BsmAI GTCTC 1 cut(s) 571
BsmFI GGGAC 2 cut(s) 492, 932
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 3 cut(s) 430, 729, 1171
Bsp119I TTCGAA 3 cut(s) 176, 363, 709
Bsp1286I GDGCHC 3 cut(s) 157, 721, 775
Bsp143I GATC 3 cut(s) 541, 739, 1279
BspACI CCGC 5 cut(s) 347, 465, 992, 1070, 1249
BspANI GGCC 3 cut(s) 430, 729, 1171
BspCNI CTCAG 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 2 cut(s) 536, 734
BspT104I TTCGAA 3 cut(s) 176, 363, 709
BsrDI GCAATG 2 cut(s) 330, 1086
BsrFI RCCGGY 1 cut(s) 1117
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 4 cut(s) 129, 379, 425, 629
BssMI GATC 3 cut(s) 541, 739, 1279
Bst2UI CCWGG 3 cut(s) 380, 554, 731
Bst4CI ACNGT 4 cut(s) 182, 426, 749, 965
Bst6I CTCTTC 2 cut(s) 327, 438
BstAPI GCANNNNNTGC 2 cut(s) 239, 492
BstBI TTCGAA 3 cut(s) 176, 363, 709
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 3 cut(s) 90, 911, 972
BstDSI CCRYGG 2 cut(s) 129, 425
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 6 cut(s) 139, 304, 356, 588, 639, 807
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 3 cut(s) 544, 742, 1282
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 3 cut(s) 541, 739, 1279
BstMWI GCNNNNNNNGC 8 cut(s) 239, 492, 938, 1051, 1076, 1142, 1254, 1263
BstNI CCWGG 3 cut(s) 380, 554, 731
BstNSI RCATGY 3 cut(s) 81, 286, 919
BstSCI CCNGG 4 cut(s) 264, 378, 552, 729
BstSFI CTRYAG 1 cut(s) 1143
BstSLI GKGCMC 3 cut(s) 157, 721, 775
BstV1I GCAGC 7 cut(s) 116, 507, 944, 1096, 1157, 1278, 1281
BstXI CCANNNNNNTGG 1 cut(s) 553
BsuRI GGCC 3 cut(s) 430, 729, 1171
BtgI CCRYGG 2 cut(s) 129, 425
BtrI CACGTC 1 cut(s) 722
BtsCI GGATG 6 cut(s) 139, 304, 356, 588, 639, 807
BtsIMutI CAGTG 1 cut(s) 1218
Cac8I GCNNGC 1 cut(s) 284
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 1 cut(s) 840
Csp6I GTAC 2 cut(s) 10, 593
CviAII CATG 8 cut(s) 78, 140, 283, 307, 475, 532, 916, 1114
CviQI GTAC 2 cut(s) 10, 593
DdeI CTNAG 3 cut(s) 90, 911, 972
DpnI GATC 3 cut(s) 543, 741, 1281
DpnII GATC 3 cut(s) 541, 739, 1279
EaeI YGGCCR 1 cut(s) 1169
Eam1104I CTCTTC 2 cut(s) 327, 438
EarI CTCTTC 2 cut(s) 327, 438
Eco32I GATATC 1 cut(s) 1225
Eco47I GGWCC 1 cut(s) 840
Eco47III AGCGCT 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 875
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoRII CCWGG 3 cut(s) 378, 552, 729
EcoRV GATATC 1 cut(s) 1225
FaeI CATG 8 cut(s) 81, 143, 286, 310, 478, 535, 919, 1117
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 2 cut(s) 492, 932
FatI CATG 8 cut(s) 77, 139, 282, 306, 474, 531, 915, 1113
FauI CCCGC 2 cut(s) 340, 472
FbaI TGATCA 1 cut(s) 1279
Fnu4HI GCNGC 9 cut(s) 105, 496, 933, 992, 1085, 1146, 1249, 1267, 1270
FokI GGATG 6 cut(s) 146, 311, 363, 595, 646, 814
Fsp4HI GCNGC 9 cut(s) 105, 496, 933, 992, 1085, 1146, 1249, 1267, 1270
FspBI CTAG 1 cut(s) 821
GlaI GCGC 1 cut(s) 108
GluI GCNGC 9 cut(s) 105, 496, 933, 992, 1085, 1146, 1249, 1267, 1270
GsaI CCCAGC 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 3 cut(s) 430, 729, 1171
HapII CCGG 3 cut(s) 265, 338, 1118
HhaI GCGC 1 cut(s) 109
Hin1II CATG 8 cut(s) 81, 143, 286, 310, 478, 535, 919, 1117
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 669, 1300
HindII GTYRAC 2 cut(s) 669, 1300
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 5 cut(s) 86, 384, 808, 1061, 1154
HpaII CCGG 3 cut(s) 265, 338, 1118
HphI GGTGA 6 cut(s) 322, 695, 946, 1022, 1060, 1266
Hpy166II GTNNAC 4 cut(s) 155, 669, 773, 1300
Hpy188III TCNNGA 4 cut(s) 203, 224, 979, 1187
Hpy8I GTNNAC 4 cut(s) 155, 669, 773, 1300
Hpy99I CGWCG 2 cut(s) 726, 1168
HpyAV CCTTC 5 cut(s) 52, 571, 621, 625, 1198
HpyCH4III ACNGT 4 cut(s) 182, 426, 749, 965
HpyCH4IV ACGT 4 cut(s) 591, 665, 721, 1014
HpyF10VI GCNNNNNNNGC 8 cut(s) 239, 492, 938, 1051, 1076, 1142, 1254, 1263
HpyF3I CTNAG 3 cut(s) 90, 911, 972
HpySE526I ACGT 4 cut(s) 591, 665, 721, 1014
Hsp92II CATG 8 cut(s) 81, 143, 286, 310, 478, 535, 919, 1117
HspAI GCGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 1279
Kzo9I GATC 3 cut(s) 541, 739, 1279
LmnI GCTCC 5 cut(s) 332, 466, 631, 900, 1183
Lsp1109I GCAGC 7 cut(s) 116, 507, 944, 1096, 1157, 1278, 1281
LweI GCATC 8 cut(s) 43, 229, 341, 573, 860, 1057, 1096, 1123
MaeI CTAG 1 cut(s) 821
MaeII ACGT 4 cut(s) 591, 665, 721, 1014
MaeIII GTNAC 3 cut(s) 763, 1010, 1259
MalI GATC 3 cut(s) 543, 741, 1281
MboI GATC 3 cut(s) 541, 739, 1279
MboII GAAGA 7 cut(s) 212, 314, 425, 817, 881, 1210, 1213
MhlI GDGCHC 3 cut(s) 157, 721, 775
MluCI AATT 8 cut(s) 21, 172, 259, 414, 598, 789, 1029, 1307
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 2 cut(s) 291, 1165
MspA1I CMGCKG 3 cut(s) 935, 1007, 1045
MspI CCGG 3 cut(s) 265, 338, 1118
MspR9I CCNGG 4 cut(s) 266, 380, 554, 731
Mva1269I GAATGC 1 cut(s) 106
MvaI CCWGG 3 cut(s) 380, 554, 731
MwoI GCNNNNNNNGC 8 cut(s) 239, 492, 938, 1051, 1076, 1142, 1254, 1263
NciI CCSGG 1 cut(s) 266
NdeII GATC 3 cut(s) 541, 739, 1279
NlaIII CATG 8 cut(s) 81, 143, 286, 310, 478, 535, 919, 1117
NmuCI GTSAC 1 cut(s) 1010
NspI RCATGY 3 cut(s) 81, 286, 919
NspV TTCGAA 3 cut(s) 176, 363, 709
OliI CACNNNNGTG 1 cut(s) 291
PaeI GCATGC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 1169
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 5 cut(s) 86, 384, 808, 1061, 1154
PkrI GCNGC 9 cut(s) 106, 497, 934, 993, 1086, 1147, 1250, 1268, 1271
PshBI ATTAAT 1 cut(s) 15
Psp6I CCWGG 3 cut(s) 378, 552, 729
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 378, 552, 729
PspPI GGNCC 1 cut(s) 840
PstI CTGCAG 1 cut(s) 1147
PvuII CAGCTG 3 cut(s) 935, 1007, 1045
RsaI GTAC 2 cut(s) 11, 594
RsaNI GTAC 2 cut(s) 10, 593
RseI CAYNNNNRTG 2 cut(s) 291, 1165
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 9 cut(s) 105, 496, 933, 992, 1085, 1146, 1249, 1267, 1270
Sau3AI GATC 3 cut(s) 541, 739, 1279
Sau96I GGNCC 1 cut(s) 840
ScrFI CCNGG 4 cut(s) 266, 380, 554, 731
SduI GDGCHC 3 cut(s) 157, 721, 775
SfaNI GCATC 8 cut(s) 43, 229, 341, 573, 860, 1057, 1096, 1123
SfcI CTRYAG 1 cut(s) 1143
SfuI TTCGAA 3 cut(s) 176, 363, 709
SinI GGWCC 1 cut(s) 840
SmiMI CAYNNNNRTG 2 cut(s) 291, 1165
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
SpeI ACTAGT 1 cut(s) 820
SphI GCATGC 1 cut(s) 286
Sse9I AATT 8 cut(s) 21, 172, 259, 414, 598, 789, 1029, 1307
SsiI CCGC 5 cut(s) 347, 465, 992, 1070, 1249
SspI AATATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 821
StyD4I CCNGG 4 cut(s) 264, 378, 552, 729
TaaI ACNGT 4 cut(s) 182, 426, 749, 965
TaiI ACGT 4 cut(s) 594, 668, 724, 1017
TaqI TCGA 5 cut(s) 51, 176, 363, 709, 724
TaqII GACCGA 1 cut(s) 857
TasI AATT 8 cut(s) 21, 172, 259, 414, 598, 789, 1029, 1307
TatI WGTACW 1 cut(s) 9
TauI GCSGC 2 cut(s) 994, 1251
TfiI GAWTC 5 cut(s) 86, 384, 808, 1061, 1154
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 1225
TseFI GTSAC 1 cut(s) 1010
TseI GCWGC 7 cut(s) 104, 495, 932, 1084, 1145, 1266, 1269
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 4 cut(s) 150, 201, 818, 879
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 1 cut(s) 1225
VneI GTGCAC 2 cut(s) 153, 771
VpaK11BI GGWCC 1 cut(s) 840
VspI ATTAAT 1 cut(s) 15
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 2 cut(s) 21, 789
XceI RCATGY 3 cut(s) 81, 286, 919
XspI CTAG 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.