pycom02g08150

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012560
Physical Location & Seq
Forward (+)
5716209 .. 5718432
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08150.5

Sequence Viewer

Length: 1341 bp
ATGGACAAGTACATTAATCAAATTTGCAGTTCCTCATCATGGTCAGATGTCGATGCGAAGGAAATACCATCTTGGGCATGTTGTGAATCTGAGCAACCGAATGAAGCGCTTCTTGGTTCCGTAGTTGTCGTGGATGAACATGAGAACAATAGTTCACCTACATATCTGACTTACTCGAACAGTTTGGAGAGCTTGGCTGCTCAAGATGTTTCATCTATTCTTCAAGATTTTGAACCAGATGCACAAGATGGTGGAAAAAATTGCGATGTTCAAAATATTGGCATGCACTATGGTGTGGATGGGAACATGGTGAGAATACCTCTTCGCTCCATTGCACCGGAAAATGTGGGATGCAATAGCTTCGAACCACTCCTCTACCAGGGATTCAATGGCGATTTTGAAACCCTTTCTCCAATTTCTCAACCGTGGCCTCTTCAATCTTATGAGGGTGTTTCTCCTGCTCCTGCTTCTGGCATGGTACAACGCAAAATGTGCAGCTTTGGAATAAATGGAGATTATGGGGATTATGTTGATTATGTCATGCCAAGCGATCCCAACAACCTGGCTGCTTTAGTCACCAACACGTTTAACGTTTATCACAAGAAGGGGACGCAGGACGTACAAAATTATCCTTTGCCTTCCTTCCCTTCTGCTCCTCGGATGTCTGGTTTGCAGTCTTTACCACAGAATACGTCAACTAACCCTGTTGGTGAATGTAATGGAAATGGAAAGCCTCGTATGAGGGCACGCCGAGGCCAGGCTACTGATCCACACAGTATTGCTGAAAGGCTTCGGAGGGAAAAAATTTCTGATAGGATGAAGAATCTGCAAGAACTTGTGCCAAAGTCTAACAGGACCGACAAGGCATCTATGCTTGATGAAATCATCGAGTATGTCAGATTTCTTCAGCTCCAACTCAAGGAACTAAGCATGAGCAAAGTTGGGGCAGCCGGGGCAGTTATCCCTCTCACCACAGACAGTCAAGCTAAGGTCGGGAATGGTTTGCCGCAGTTGCCATCAATCGGGCAAGCAGCTGACGTTTCTTTCGACGAAATTGCTTTAGAACAACTGGTGAGGCTGATGGAATCAGATGCGGACAAGGCACTGCAGCATCTCCAAAGCGAAGGTTTCTGCCTCGTGCCGGTTGCTCTTGCTGATGCCATTTCTGCAGCAAAGGAATCATCATCGTCGCGGCCGTCAGCTCCCGTTCCTGATGATTGGAAGAAGAAGGAAACCAGTTTCGCTGATATCGGCGTTGTTCAGAATAACAGCCGCAGTAGCAGTAACAGCAGCAGCTCACCTGATCGGATAAAACGAGAAGTCAACAACAAATTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

447

Amino Acids

48.63

Weight (kDa)

4.83

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1192
AciI CCGC 4 cut(s) 1007, 1094, 1192, 1273
AclI AACGTT 1 cut(s) 591
AclWI GGATC 2 cut(s) 545, 761
AcoI YGGCCR 1 cut(s) 1193
AcsI RAATTY 2 cut(s) 21, 804
AcuI CTGAAG 1 cut(s) 890
AfaI GTAC 3 cut(s) 11, 480, 621
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 7 cut(s) 39, 379, 470, 757, 919, 1022, 1141
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 6 cut(s) 224, 233, 272, 388, 401, 437
AjnI CCWGG 3 cut(s) 378, 561, 756
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
AleI CACNNNNGTG 1 cut(s) 291
AluBI AGCT 8 cut(s) 192, 360, 498, 910, 986, 1034, 1202, 1296
AluI AGCT 8 cut(s) 192, 360, 498, 910, 986, 1034, 1202, 1296
AlwI GGATC 2 cut(s) 545, 761
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 3 cut(s) 428, 754, 1193
ApeKI GCWGC 9 cut(s) 197, 495, 566, 947, 1031, 1108, 1169, 1290, 1293
ApoI RAATTY 2 cut(s) 21, 804
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
AseI ATTAAT 1 cut(s) 15
Asp700I GAANNNNTTC 2 cut(s) 108, 789
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 855
AsuC2I CCSGG 1 cut(s) 952
AsuHPI GGTGA 7 cut(s) 147, 322, 568, 722, 961, 1084, 1290
AsuII TTCGAA 1 cut(s) 363
AvaII GGWCC 1 cut(s) 855
BaeGI GKGCMC 1 cut(s) 748
BauI CACGAG 1 cut(s) 1136
BbvI GCAGC 9 cut(s) 184, 507, 553, 959, 1043, 1120, 1181, 1302, 1305
BccI CCATC 5 cut(s) 76, 242, 293, 1024, 1075
BceAI ACGGC 1 cut(s) 1180
BcgI CGANNNNNNTGC 4 cut(s) 343, 377, 1037, 1071
BciT130I CCWGG 3 cut(s) 380, 563, 758
BcnI CCSGG 1 cut(s) 952
BfmI CTRYAG 2 cut(s) 1106, 1167
BfoI RGCGCY 1 cut(s) 110
Bme1390I CCNGG 4 cut(s) 380, 563, 758, 952
Bme18I GGWCC 1 cut(s) 855
BmgT120I GGNCC 1 cut(s) 855
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 4 cut(s) 380, 563, 758, 952
BmsI GCATC 7 cut(s) 43, 229, 341, 875, 1081, 1120, 1147
BplI GAGNNNNNCTC 2 cut(s) 304, 336
Bpu10I CCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 186, 902
BpuMI CCSGG 1 cut(s) 952
BsaJI CCNNGG 5 cut(s) 379, 425, 656, 751, 951
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 394, 424
Bsc4I CCNNNNNNNGG 7 cut(s) 39, 379, 470, 757, 919, 1022, 1141
Bse118I RCCGGY 1 cut(s) 1141
Bse1I ACTGG 2 cut(s) 1074, 1236
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 3 cut(s) 380, 563, 758
BseDI CCNNGG 5 cut(s) 379, 425, 656, 751, 951
BseGI GGATG 5 cut(s) 139, 304, 356, 666, 822
BseLI CCNNNNNNNGG 7 cut(s) 39, 379, 470, 757, 919, 1022, 1141
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 1074, 1236
BseRI GAGGAG 2 cut(s) 362, 645
BseSI GKGCMC 1 cut(s) 748
BseX3I CGGCCG 1 cut(s) 1193
BseXI GCAGC 9 cut(s) 184, 507, 553, 959, 1043, 1120, 1181, 1302, 1305
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 1192
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 3 cut(s) 430, 756, 1195
BsiEI CGRYCG 1 cut(s) 1196
BsiSI CCGG 3 cut(s) 338, 951, 1142
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 7 cut(s) 39, 379, 470, 757, 919, 1022, 1141
BsmFI GGGAC 1 cut(s) 622
BsnI GGCC 3 cut(s) 430, 756, 1195
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 3 cut(s) 550, 766, 1303
BspACI CCGC 4 cut(s) 1007, 1094, 1192, 1273
BspANI GGCC 3 cut(s) 430, 756, 1195
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 1 cut(s) 1192
BspLI GGNNCC 1 cut(s) 118
BspMAI CTGCAG 2 cut(s) 1110, 1171
BspPI GGATC 2 cut(s) 545, 761
BspT104I TTCGAA 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 1141
BsrI ACTGG 2 cut(s) 1074, 1236
BssAI RCCGGY 1 cut(s) 1141
BssECI CCNNGG 5 cut(s) 379, 425, 656, 751, 951
BssMI GATC 3 cut(s) 550, 766, 1303
BssSI CACGAG 1 cut(s) 1136
Bst2BI CACGAG 1 cut(s) 1136
Bst2UI CCWGG 3 cut(s) 380, 563, 758
Bst4CI ACNGT 4 cut(s) 182, 426, 776, 980
Bst6I CTCTTC 2 cut(s) 327, 438
BstAPI GCANNNNNTGC 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 284, 748, 1029
BstDEI CTNAG 3 cut(s) 90, 926, 987
BstDSI CCRYGG 1 cut(s) 425
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 5 cut(s) 139, 304, 356, 666, 822
BstFNI CGCG 1 cut(s) 1192
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 3 cut(s) 553, 769, 1306
BstMBI GATC 3 cut(s) 550, 766, 1303
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 7 cut(s) 492, 953, 1012, 1100, 1166, 1278, 1287
BstNI CCWGG 3 cut(s) 380, 563, 758
BstNSI RCATGY 2 cut(s) 81, 286
BstSCI CCNGG 4 cut(s) 378, 561, 756, 950
BstSFI CTRYAG 2 cut(s) 1106, 1167
BstSLI GKGCMC 1 cut(s) 748
BstUI CGCG 1 cut(s) 1192
BstV1I GCAGC 9 cut(s) 184, 507, 553, 959, 1043, 1120, 1181, 1302, 1305
BstXI CCANNNNNNTGG 1 cut(s) 562
BstZI CGGCCG 1 cut(s) 1193
BsuRI GGCC 3 cut(s) 430, 756, 1195
BtgI CCRYGG 1 cut(s) 425
BtgZI GCGATG 1 cut(s) 279
BtsCI GGATG 5 cut(s) 139, 304, 356, 666, 822
BtsI GCAGTG 1 cut(s) 1103
BtsIMutI CAGTG 1 cut(s) 1103
Cac8I GCNNGC 3 cut(s) 284, 748, 1029
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 1141
Cfr13I GGNCC 1 cut(s) 855
CseI GACGC 1 cut(s) 619
Csp6I GTAC 3 cut(s) 10, 479, 620
CviAII CATG 8 cut(s) 39, 78, 140, 283, 307, 475, 541, 931
CviQI GTAC 3 cut(s) 10, 479, 620
DdeI CTNAG 3 cut(s) 90, 926, 987
DpnI GATC 3 cut(s) 552, 768, 1305
DpnII GATC 3 cut(s) 550, 766, 1303
EaeI YGGCCR 1 cut(s) 1193
EagI CGGCCG 1 cut(s) 1193
Eam1104I CTCTTC 2 cut(s) 327, 438
EarI CTCTTC 2 cut(s) 327, 438
EclXI CGGCCG 1 cut(s) 1193
Eco32I GATATC 1 cut(s) 1249
Eco47I GGWCC 1 cut(s) 855
Eco47III AGCGCT 1 cut(s) 108
Eco52I CGGCCG 1 cut(s) 1193
Eco57I CTGAAG 1 cut(s) 890
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoRII CCWGG 3 cut(s) 378, 561, 756
EcoRV GATATC 1 cut(s) 1249
FaeI CATG 8 cut(s) 42, 81, 143, 286, 310, 478, 544, 934
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 1 cut(s) 622
FatI CATG 8 cut(s) 38, 77, 139, 282, 306, 474, 540, 930
FokI GGATG 5 cut(s) 146, 311, 363, 673, 829
GlaI GCGC 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 3 cut(s) 430, 756, 1195
HapII CCGG 3 cut(s) 338, 951, 1142
HgaI GACGC 1 cut(s) 619
HhaI GCGC 1 cut(s) 109
Hin1II CATG 8 cut(s) 42, 81, 143, 286, 310, 478, 544, 934
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 696, 1324
HindII GTYRAC 2 cut(s) 696, 1324
HinfI GANTC 5 cut(s) 86, 384, 823, 1085, 1178
HpaII CCGG 3 cut(s) 338, 951, 1142
HphI GGTGA 7 cut(s) 147, 322, 568, 722, 961, 1084, 1290
Hpy166II GTNNAC 3 cut(s) 155, 696, 1324
Hpy188III TCNNGA 4 cut(s) 203, 224, 994, 1211
Hpy8I GTNNAC 3 cut(s) 155, 696, 1324
Hpy99I CGWCG 2 cut(s) 1052, 1192
HpyAV CCTTC 7 cut(s) 52, 598, 648, 652, 657, 1118, 1222
HpyCH4III ACNGT 4 cut(s) 182, 426, 776, 980
HpyCH4IV ACGT 5 cut(s) 584, 591, 618, 692, 1038
HpyF10VI GCNNNNNNNGC 7 cut(s) 492, 953, 1012, 1100, 1166, 1278, 1287
HpyF3I CTNAG 3 cut(s) 90, 926, 987
HpySE526I ACGT 5 cut(s) 584, 591, 618, 692, 1038
Hsp92II CATG 8 cut(s) 42, 81, 143, 286, 310, 478, 544, 934
HspAI GCGC 1 cut(s) 107
Kzo9I GATC 3 cut(s) 550, 766, 1303
LmnI GCTCC 5 cut(s) 332, 466, 658, 915, 1207
Lsp1109I GCAGC 9 cut(s) 184, 507, 553, 959, 1043, 1120, 1181, 1302, 1305
LweI GCATC 7 cut(s) 43, 229, 341, 875, 1081, 1120, 1147
MaeII ACGT 5 cut(s) 584, 591, 618, 692, 1038
MaeIII GTNAC 2 cut(s) 574, 1283
MalI GATC 3 cut(s) 552, 768, 1305
MboI GATC 3 cut(s) 550, 766, 1303
MboII GAAGA 7 cut(s) 212, 314, 425, 832, 896, 1234, 1237
MhlI GDGCHC 1 cut(s) 748
MluCI AATT 7 cut(s) 21, 259, 414, 625, 804, 1053, 1331
MmeI TCCRAC 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 108, 789
MseI TTAA 2 cut(s) 15, 588
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 1034
MspI CCGG 3 cut(s) 338, 951, 1142
MspR9I CCNGG 4 cut(s) 380, 563, 758, 952
MvaI CCWGG 3 cut(s) 380, 563, 758
MvnI CGCG 1 cut(s) 1192
MwoI GCNNNNNNNGC 7 cut(s) 492, 953, 1012, 1100, 1166, 1278, 1287
NciI CCSGG 1 cut(s) 952
NdeII GATC 3 cut(s) 550, 766, 1303
NlaIII CATG 8 cut(s) 42, 81, 143, 286, 310, 478, 544, 934
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 776
NmuCI GTSAC 1 cut(s) 574
NspI RCATGY 2 cut(s) 81, 286
NspV TTCGAA 1 cut(s) 363
OliI CACNNNNGTG 1 cut(s) 291
PaeI GCATGC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 3 cut(s) 1044, 1193, 1312
PdmI GAANNNNTTC 2 cut(s) 108, 789
PfeI GAWTC 5 cut(s) 86, 384, 823, 1085, 1178
PshBI ATTAAT 1 cut(s) 15
Psp1406I AACGTT 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 378, 561, 756
PspGI CCWGG 3 cut(s) 378, 561, 756
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 855
PstI CTGCAG 2 cut(s) 1110, 1171
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 3 cut(s) 11, 480, 621
RsaNI GTAC 3 cut(s) 10, 479, 620
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 2 cut(s) 15, 588
Sau3AI GATC 3 cut(s) 550, 766, 1303
Sau96I GGNCC 1 cut(s) 855
ScrFI CCNGG 4 cut(s) 380, 563, 758, 952
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 7 cut(s) 43, 229, 341, 875, 1081, 1120, 1147
SfcI CTRYAG 2 cut(s) 1106, 1167
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 2 cut(s) 201, 917
SmoI CTYRAG 2 cut(s) 201, 917
SphI GCATGC 1 cut(s) 286
Sse9I AATT 7 cut(s) 21, 259, 414, 625, 804, 1053, 1331
SsiI CCGC 4 cut(s) 1007, 1094, 1192, 1273
SspI AATATT 1 cut(s) 277
StyD4I CCNGG 4 cut(s) 378, 561, 756, 950
TaaI ACNGT 4 cut(s) 182, 426, 776, 980
TaiI ACGT 5 cut(s) 587, 594, 621, 695, 1041
TaqI TCGA 5 cut(s) 51, 176, 363, 888, 1047
TaqII GACCGA 1 cut(s) 872
TasI AATT 7 cut(s) 21, 259, 414, 625, 804, 1053, 1331
TatI WGTACW 1 cut(s) 9
TauI GCSGC 3 cut(s) 1009, 1195, 1275
TfiI GAWTC 5 cut(s) 86, 384, 823, 1085, 1178
Tru1I TTAA 2 cut(s) 15, 588
Tru9I TTAA 2 cut(s) 15, 588
TscAI CASTG 1 cut(s) 1110
TseFI GTSAC 1 cut(s) 574
TseI GCWGC 9 cut(s) 197, 495, 566, 947, 1031, 1108, 1169, 1290, 1293
Tsp45I GTSAC 1 cut(s) 574
TspDTI ATGAA 5 cut(s) 117, 150, 201, 833, 894
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 1 cut(s) 1110
VpaK11BI GGWCC 1 cut(s) 855
VspI ATTAAT 1 cut(s) 15
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 2 cut(s) 21, 804
XceI RCATGY 2 cut(s) 81, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XmnI GAANNNNTTC 2 cut(s) 108, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.