Rh2BG106000

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012560
Physical Location & Seq
Forward (+)
8893590 .. 8896854
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG106000.1

Sequence Viewer

Length: 1296 bp
ATGATGGACGAGATTCTTGATCATTTTTTCAGTTCCTCATCATGGACAGATGTGGATGTAAAGGAAAGATCATCTCTGGACTGTTACGAAAATCCACCAACAAACGAAATGTTTCTCAGTTCACTTGGAGTAATTGAAGATGACAGCAATAACTCTGCTACAAATCTGAGCAACTCAAACCACAGCATGGAGAGCATGGCTGCACAGTCTGCTAATTCATCTGTTGCTCATGATTTTGAGTCTGATGCACAAGACAATGGCAACAATTGCGCGGCCAACTTCTCGATCAATGATCAAATGGGCCTGCAATATGATTTGGATGGTAACATGGCGAGATCAACATCTGTTTCGTCCCTAGAGCCACAAAATGTGGGAGGCAATTGTTGTGAACAGTCTGTCTTTCAGAGACTCAATGGAGATATTGAAACCCTTTCTCCAATTTCTCAGCTGTGGCCTCTTCAAGCTTATGAGGGTGTCTCTTCTCCTCTTTGTCCTCCTGGTATGGGACAACAGAAGCTGTGCAGCTTTGGTAGTAATGATATTTATGAGTTATCTGATTTAGTCACCACAGAGAAGGTAAGCCAGGAGATGGGAAATTATCCTTTGCCCTCTTTTGCTTCTGGGCCTCAGATTCCAATGGCAATGGCTGGATTGCAGTCTTTCCCACAGACTACATCAACTAACCCCTCTGCTGAATGTACTGGACCTAGAAAGCCTCGTATGAGGGCACGCCGAGGCCAGGCAACTGATCCACACAGTATTGCTGAAAGGCTTCGGAGAGAGAAAATTGCTGACAGAATGAAGAATCTGCAAGAACTTGTACCAAAGTCTAACAAGACTGACAAGGCATCTATGCTTGATGAAATCATCGAGTATGTCAAGTTTCTTCAGCAACAACTCAAGGTTCTAAGCACACGCAGATTGGGAGCTACAGGGGCAGTTATCTTGGATAATCAGGCTAAGGGGACCAATGGGATTTCACAGTCTCCATCACTTGGTCAAGTAGCTCATGACATTTCTTTCAATGAAATTGCTTTTGAACAAGTACTGAAACTGATGGAAACCGATGTAACCAAGGCAATGCATTATCTCCAAAGCAAAGGTCTCTGCATGATGCCCGTCGCTCTTGCCAATGCCATATTGGCCGAAAAGGAATCATCACCGGCTCCTGATTCTGCCGAGAATTTGAAAAACTCCGGTTTTACTGATGATGGTTTTGTTCACGACCCCAGCAGCAGCAGTAGCAGTAACAGCAACACCTTGCCTGGCACCGACAAAACGGCTGTCAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-C3HC4

Protein Analysis

431

Amino Acids

46.74

Weight (kDa)

4.67

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 252 - 297 3.5e-10 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 256 - 302 6.2e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1268
AccB7I CCANNNNNTGG 3 cut(s) 187, 589, 995
AccII CGCG 1 cut(s) 272
AciI CCGC 1 cut(s) 272
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 2 cut(s) 273, 1143
AcsI RAATTY 1 cut(s) 1183
AcuI CTGAAG 1 cut(s) 872
AfaI GTAC 3 cut(s) 700, 822, 1047
AfiI CCNNNNNNNGG 6 cut(s) 42, 187, 503, 589, 739, 995
AgsI TTSAA 6 cut(s) 137, 425, 461, 1024, 1040, 1189
AjnI CCWGG 4 cut(s) 496, 582, 738, 1264
AluBI AGCT 6 cut(s) 448, 464, 517, 525, 929, 1007
AluI AGCT 6 cut(s) 448, 464, 517, 525, 929, 1007
Alw26I GTCTC 4 cut(s) 400, 481, 990, 1109
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 1 cut(s) 517
AoxI GGCC 6 cut(s) 273, 301, 452, 623, 736, 1143
ApeKI GCWGC 4 cut(s) 200, 522, 1233, 1236
ApoI RAATTY 1 cut(s) 1183
ArsI GACNNNNNNTTYG 3 cut(s) 1087, 1119, 1269
Asp700I GAANNNNTTC 2 cut(s) 111, 771
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 4 cut(s) 301, 623, 704, 966
AsuHPI GGTGA 2 cut(s) 556, 1152
AvaII GGWCC 2 cut(s) 704, 966
BaeGI GKGCMC 1 cut(s) 730
BanI GGYRCC 1 cut(s) 1268
BbvI GCAGC 4 cut(s) 187, 534, 1245, 1248
BccI CCATC 5 cut(s) 314, 583, 997, 1051, 1205
BciT130I CCWGG 4 cut(s) 498, 584, 740, 1266
BclI TGATCA 2 cut(s) 19, 292
BcoDI GTCTC 4 cut(s) 400, 481, 990, 1109
BfaI CTAG 2 cut(s) 356, 708
BfmI CTRYAG 1 cut(s) 930
BglI GCCNNNNNGGC 1 cut(s) 1142
BisI GCNGC 5 cut(s) 201, 273, 523, 1234, 1237
BlsI GCNGC 5 cut(s) 202, 274, 524, 1235, 1238
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 4 cut(s) 498, 584, 740, 1266
Bme18I GGWCC 2 cut(s) 704, 966
BmgT120I GGNCC 4 cut(s) 301, 623, 704, 966
BmiI GGNNCC 3 cut(s) 967, 1167, 1270
BmrFI CCNGG 4 cut(s) 498, 584, 740, 1266
BmsI GCATC 3 cut(s) 235, 857, 1104
BplI GAGNNNNNCTC 2 cut(s) 461, 493
Bpu10I CCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 340
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 2 cut(s) 733, 1074
BsaWI WCCGGW 1 cut(s) 1196
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 187, 503, 589, 739, 995
Bse118I RCCGGY 1 cut(s) 1162
Bse1I ACTGG 1 cut(s) 706
Bse3DI GCAATG 2 cut(s) 648, 1086
Bse8I GATNNNNATC 1 cut(s) 340
BseBI CCWGG 4 cut(s) 498, 584, 740, 1266
BseDI CCNNGG 2 cut(s) 733, 1074
BseGI GGATG 2 cut(s) 61, 325
BseJI GATNNNNATC 1 cut(s) 340
BseLI CCNNNNNNNGG 6 cut(s) 42, 187, 503, 589, 739, 995
BseMI GCAATG 2 cut(s) 648, 1086
BseMII CTCAG 4 cut(s) 130, 158, 458, 641
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 474
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 4 cut(s) 187, 534, 1245, 1248
BseYI CCCAGC 1 cut(s) 1229
BsgI GTGCAG 2 cut(s) 186, 541
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 6 cut(s) 275, 303, 454, 625, 738, 1145
BshNI GGYRCC 1 cut(s) 1268
BsiSI CCGG 2 cut(s) 1163, 1197
BslFI GGGAC 3 cut(s) 337, 519, 979
BslI CCNNNNNNNGG 6 cut(s) 42, 187, 503, 589, 739, 995
BsmAI GTCTC 4 cut(s) 400, 481, 990, 1109
BsmFI GGGAC 3 cut(s) 337, 519, 979
BsnI GGCC 6 cut(s) 275, 303, 454, 625, 738, 1145
Bso31I GGTCTC 1 cut(s) 1109
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 6 cut(s) 19, 68, 285, 292, 335, 748
BspACI CCGC 1 cut(s) 272
BspANI GGCC 6 cut(s) 275, 303, 454, 625, 738, 1145
BspCNI CTCAG 4 cut(s) 129, 159, 457, 640
BspFNI CGCG 1 cut(s) 272
BspHI TCATGA 2 cut(s) 229, 1009
BspLI GGNNCC 3 cut(s) 967, 1167, 1270
BspPI GGATC 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 1268
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 2 cut(s) 648, 1086
BsrFI RCCGGY 1 cut(s) 1162
BsrI ACTGG 1 cut(s) 706
BssAI RCCGGY 1 cut(s) 1162
BssECI CCNNGG 2 cut(s) 733, 1074
BssMI GATC 6 cut(s) 19, 68, 285, 292, 335, 748
BssT1I CCWWGG 1 cut(s) 1074
Bst2UI CCWGG 4 cut(s) 498, 584, 740, 1266
Bst4CI ACNGT 5 cut(s) 83, 207, 393, 758, 984
Bst6I CTCTTC 2 cut(s) 462, 484
BstAPI GCANNNNNTGC 2 cut(s) 209, 267
BstC8I GCNNGC 2 cut(s) 305, 730
BstDEI CTNAG 6 cut(s) 116, 167, 444, 627, 908, 960
BstF5I GGATG 2 cut(s) 61, 325
BstFNI CGCG 1 cut(s) 272
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 6 cut(s) 22, 71, 288, 295, 338, 751
BstMAI GTCTC 4 cut(s) 400, 481, 990, 1109
BstMBI GATC 6 cut(s) 19, 68, 285, 292, 335, 748
BstMWI GCNNNNNNNGC 7 cut(s) 192, 209, 267, 935, 1142, 1242, 1251
BstNI CCWGG 4 cut(s) 498, 584, 740, 1266
BstSCI CCNGG 4 cut(s) 496, 582, 738, 1264
BstSFI CTRYAG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 730
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 187, 534, 1245, 1248
BsuRI GGCC 6 cut(s) 275, 303, 454, 625, 738, 1145
BtsCI GGATG 2 cut(s) 61, 325
Cac8I GCNNGC 2 cut(s) 305, 730
CaiI CAGNNNCTG 1 cut(s) 517
CciI TCATGA 2 cut(s) 229, 1009
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 1162
Cfr13I GGNCC 4 cut(s) 301, 623, 704, 966
Csp6I GTAC 3 cut(s) 699, 821, 1046
CviAII CATG 8 cut(s) 42, 187, 196, 230, 328, 1010, 1111, 1293
CviQI GTAC 3 cut(s) 699, 821, 1046
DdeI CTNAG 6 cut(s) 116, 167, 444, 627, 908, 960
DpnI GATC 6 cut(s) 21, 70, 287, 294, 337, 750
DpnII GATC 6 cut(s) 19, 68, 285, 292, 335, 748
EaeI YGGCCR 2 cut(s) 273, 1143
Eam1104I CTCTTC 2 cut(s) 462, 484
EarI CTCTTC 2 cut(s) 462, 484
Eco130I CCWWGG 1 cut(s) 1074
Eco31I GGTCTC 1 cut(s) 1109
Eco47I GGWCC 2 cut(s) 704, 966
Eco57I CTGAAG 1 cut(s) 872
EcoRII CCWGG 4 cut(s) 496, 582, 738, 1264
EcoT14I CCWWGG 1 cut(s) 1074
EcoT22I ATGCAT 1 cut(s) 1086
ErhI CCWWGG 1 cut(s) 1074
FaeI CATG 8 cut(s) 45, 190, 199, 233, 331, 1013, 1114, 1296
FaqI GGGAC 3 cut(s) 337, 519, 979
FatI CATG 8 cut(s) 41, 186, 195, 229, 327, 1009, 1110, 1292
FbaI TGATCA 2 cut(s) 19, 292
Fnu4HI GCNGC 5 cut(s) 201, 273, 523, 1234, 1237
FokI GGATG 2 cut(s) 68, 332
Fsp4HI GCNGC 5 cut(s) 201, 273, 523, 1234, 1237
FspBI CTAG 2 cut(s) 356, 708
GlaI GCGC 1 cut(s) 271
GluI GCNGC 5 cut(s) 201, 273, 523, 1234, 1237
GsaI CCCAGC 1 cut(s) 1233
HaeIII GGCC 6 cut(s) 275, 303, 454, 625, 738, 1145
HapII CCGG 2 cut(s) 1163, 1197
HhaI GCGC 1 cut(s) 272
Hin1II CATG 8 cut(s) 45, 190, 199, 233, 331, 1013, 1114, 1296
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 7 cut(s) 13, 239, 408, 631, 805, 1154, 1172
HpaII CCGG 2 cut(s) 1163, 1197
HphI GGTGA 2 cut(s) 556, 1152
Hpy166II GTNNAC 3 cut(s) 122, 389, 1222
Hpy188I TCNGA 6 cut(s) 168, 244, 405, 556, 630, 777
Hpy188III TCNNGA 7 cut(s) 17, 77, 230, 283, 1010, 1169, 1223
Hpy8I GTNNAC 3 cut(s) 122, 389, 1222
Hpy99I CGWCG 1 cut(s) 1124
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 5 cut(s) 83, 207, 393, 758, 984
HpyCH4V TGCA 8 cut(s) 203, 248, 307, 522, 655, 811, 1084, 1110
HpyF10VI GCNNNNNNNGC 7 cut(s) 192, 209, 267, 935, 1142, 1242, 1251
HpyF3I CTNAG 6 cut(s) 116, 167, 444, 627, 908, 960
Hsp92II CATG 8 cut(s) 45, 190, 199, 233, 331, 1013, 1114, 1296
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 2 cut(s) 19, 292
Kzo9I GATC 6 cut(s) 19, 68, 285, 292, 335, 748
LmnI GCTCC 2 cut(s) 926, 1171
Lsp1109I GCAGC 4 cut(s) 187, 534, 1245, 1248
LweI GCATC 3 cut(s) 235, 857, 1104
MaeI CTAG 2 cut(s) 356, 708
MaeIII GTNAC 5 cut(s) 83, 323, 562, 1069, 1247
MalI GATC 6 cut(s) 21, 70, 287, 294, 337, 750
MboI GATC 6 cut(s) 19, 68, 285, 292, 335, 748
MboII GAAGA 5 cut(s) 149, 449, 471, 814, 878
MfeI CAATTG 2 cut(s) 265, 379
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 9 cut(s) 132, 214, 265, 379, 438, 595, 786, 1029, 1183
MlyI GAGTC 2 cut(s) 248, 402
Mph1103I ATGCAT 1 cut(s) 1086
MroXI GAANNNNTTC 2 cut(s) 111, 771
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 2 cut(s) 1163, 1197
MspR9I CCNGG 4 cut(s) 498, 584, 740, 1266
MunI CAATTG 2 cut(s) 265, 379
MvaI CCWGG 4 cut(s) 498, 584, 740, 1266
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 7 cut(s) 192, 209, 267, 935, 1142, 1242, 1251
NdeII GATC 6 cut(s) 19, 68, 285, 292, 335, 748
NlaIII CATG 8 cut(s) 45, 190, 199, 233, 331, 1013, 1114, 1296
NlaIV GGNNCC 3 cut(s) 967, 1167, 1270
NmeAIII GCCGAG 2 cut(s) 758, 1204
NmuCI GTSAC 1 cut(s) 562
NsiI ATGCAT 1 cut(s) 1086
PagI TCATGA 2 cut(s) 229, 1009
PdmI GAANNNNTTC 2 cut(s) 111, 771
PfeI GAWTC 5 cut(s) 13, 631, 805, 1154, 1172
PflMI CCANNNNNTGG 3 cut(s) 187, 589, 995
PkrI GCNGC 5 cut(s) 202, 274, 524, 1235, 1238
PleI GAGTC 2 cut(s) 247, 402
PpsI GAGTC 2 cut(s) 247, 402
Psp6I CCWGG 4 cut(s) 496, 582, 738, 1264
PspFI CCCAGC 1 cut(s) 1229
PspGI CCWGG 4 cut(s) 496, 582, 738, 1264
PspN4I GGNNCC 3 cut(s) 967, 1167, 1270
PspPI GGNCC 4 cut(s) 301, 623, 704, 966
PstNI CAGNNNCTG 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 3 cut(s) 700, 822, 1047
RsaNI GTAC 3 cut(s) 699, 821, 1046
SatI GCNGC 5 cut(s) 201, 273, 523, 1234, 1237
Sau3AI GATC 6 cut(s) 19, 68, 285, 292, 335, 748
Sau96I GGNCC 4 cut(s) 301, 623, 704, 966
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 2 cut(s) 248, 402
ScrFI CCNGG 4 cut(s) 498, 584, 740, 1266
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 3 cut(s) 235, 857, 1104
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 2 cut(s) 704, 966
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
Sse9I AATT 9 cut(s) 132, 214, 265, 379, 438, 595, 786, 1029, 1183
SsiI CCGC 1 cut(s) 272
SspMI CTAG 2 cut(s) 356, 708
StyD4I CCNGG 4 cut(s) 496, 582, 738, 1264
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 5 cut(s) 83, 207, 393, 758, 984
TaqI TCGA 2 cut(s) 284, 870
TasI AATT 9 cut(s) 132, 214, 265, 379, 438, 595, 786, 1029, 1183
TatI WGTACW 2 cut(s) 698, 1045
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 5 cut(s) 13, 631, 805, 1154, 1172
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 4 cut(s) 200, 522, 1233, 1236
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 4 cut(s) 207, 815, 876, 1041
Van91I CCANNNNNTGG 3 cut(s) 187, 589, 995
VpaK11BI GGWCC 2 cut(s) 704, 966
XapI RAATTY 1 cut(s) 1183
XcmI CCANNNNNNNNNTGG 1 cut(s) 1138
XmnI GAANNNNTTC 2 cut(s) 111, 771
XspI CTAG 2 cut(s) 356, 708
ZrmI AGTACT 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.