RLG00000016624

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012560
Physical Location & Seq
Forward (+)
8334206 .. 8336458
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016624

Sequence Viewer

Length: 1296 bp
ATGGACGAGATTCTTGATCATTTTTTCAGTTCCTCATCATGGACAGATGTGGATGTAAAGGAAAGATCATCTTTGGACTGTTACGAAAATCCACCAACAAACGAAATGTTTCTCAGTTCACTTGGAGTAATTGAAGATGACAGCAATAACTCTGCTACAAATCAGAGCAACTCAAACCACAGCATGGAGAGCATGGCTGCACAGTCTGCTAATTCATCTGTTGCTCATGATTTTGAGTCTGATGCACAAGACAATTGCAACAATTGCGCCGCCAACTTCTCGATTAATGATCAAATGGGCCTGCAATATGATTTGGATGGTAACATGGCGAGATCAACATCTGTTTCGTCCCTAGAGCCACAAAATGTGGGAGGCAATTGTTGTGAACAGTCCGTCTTTCAGAGACTCAATGGAGATATTGAAACGCTTTCTCCAATTTCTCAGCTGTGGCCTCTTCAAGCTTATGAGGGTGTCTCTTCTCCTCTTTGTCCTCCTGGTGTGGGACAACAGAAGCTGTGCCGCTTTGGTAGTAATGATATTTATGAGTTATCTGATTTAGTCACCACAGAGAAGGCAACCCAGGAGATGGGAAATTATCCTTTGCCCTGTTTTGCTTCTGGGCCTCAGATTCCAATGGCAATGACTGGATTGCAGTCTTTCCCACAGACTACATCAACTAACCCCTCTGCTGAATGTACTGGACCTAGAAAGCCTCGTATGAGGGCACGCCGAGGCCAGGCAACTGATCCACACAGTATTGCTGAAAGGCTTCGGAGAGAGAAAATTGCTGACAGAATGAAGAATCTGCAAGAACTTGTACCAAAGTCTAACAAGACTGACAAGGCATCTATGCTTGATGAAATCATCGAGTATGTCAAGTTTCTTCAGCAACAACTCAAGGTTCTAAGCGCACGCAGATTGGGAGCTACAGGGGCAGTTATCTTGGATAATCAGGCTAAGGGGACCAATGGGATTTCACAGTCTCCATCACTTGGTCAAGTAGCTCATGACATTTCTTTCAATGAAATTGCTTTTGAACAAGTACTGAAACTGATGGAAACCGATGTAACCAAGGCAATGCATTATCTCCAAAGCAAAGGTCTCTGCATGATGCCCGTCGCTCTTGCCAACGCCATATTGGCCGAAAAGGAATCATCACCGGCTCCTGATTCTGCCGAGAATTTGAAGAACTCCGGTTTTACTGATGATGGTTTTGTTCACAATCCCAGCAGCAGCAGCAGCAGCAGTAACAGCAACACCTTGCCTGGCACTGACAAAACGGCTGTCAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-C3HC4

Protein Analysis

432

Amino Acids

46.79

Weight (kDa)

4.74

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 251 - 296 3.5e-10 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 255 - 301 7.3e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 184, 586, 992
AciI CCGC 2 cut(s) 270, 520
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 1 cut(s) 1180
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 3 cut(s) 697, 819, 1044
AfiI CCNNNNNNNGG 6 cut(s) 39, 184, 500, 586, 736, 992
AgsI TTSAA 6 cut(s) 134, 422, 458, 1021, 1037, 1186
AjnI CCWGG 4 cut(s) 493, 579, 735, 1264
AluBI AGCT 5 cut(s) 445, 461, 514, 926, 1004
AluI AGCT 5 cut(s) 445, 461, 514, 926, 1004
Alw26I GTCTC 4 cut(s) 397, 478, 987, 1106
AlwI GGATC 1 cut(s) 740
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 5 cut(s) 298, 449, 620, 733, 1140
ApeKI GCWGC 6 cut(s) 197, 1230, 1233, 1236, 1239, 1242
ApoI RAATTY 1 cut(s) 1180
ArsI GACNNNNNNTTYG 3 cut(s) 1084, 1116, 1269
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 2 cut(s) 108, 768
AspLEI GCGC 2 cut(s) 269, 911
AspS9I GGNCC 4 cut(s) 298, 620, 701, 963
AsuHPI GGTGA 2 cut(s) 553, 1149
AvaII GGWCC 2 cut(s) 701, 963
BaeGI GKGCMC 1 cut(s) 727
BbvI GCAGC 6 cut(s) 184, 1242, 1245, 1248, 1251, 1254
BccI CCATC 5 cut(s) 311, 580, 994, 1048, 1202
BciT130I CCWGG 4 cut(s) 495, 581, 737, 1266
BclI TGATCA 2 cut(s) 16, 289
BcoDI GTCTC 4 cut(s) 397, 478, 987, 1106
BfaI CTAG 2 cut(s) 353, 705
BfmI CTRYAG 1 cut(s) 927
BglI GCCNNNNNGGC 1 cut(s) 1139
BisI GCNGC 8 cut(s) 198, 270, 520, 1231, 1234, 1237, 1240, 1243
BlsI GCNGC 8 cut(s) 199, 271, 521, 1232, 1235, 1238, 1241, 1244
BmcAI AGTACT 1 cut(s) 1044
Bme1390I CCNGG 4 cut(s) 495, 581, 737, 1266
Bme18I GGWCC 2 cut(s) 701, 963
BmgT120I GGNCC 4 cut(s) 298, 620, 701, 963
BmiI GGNNCC 2 cut(s) 964, 1164
BmrFI CCNGG 4 cut(s) 495, 581, 737, 1266
BmsI GCATC 3 cut(s) 232, 854, 1101
BplI GAGNNNNNCTC 2 cut(s) 458, 490
Bpu10I CCTNAGC 1 cut(s) 957
BpuEI CTTGAG 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 3 cut(s) 579, 730, 1071
BsaWI WCCGGW 1 cut(s) 1193
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 184, 500, 586, 736, 992
Bse118I RCCGGY 1 cut(s) 1159
Bse1I ACTGG 2 cut(s) 649, 703
Bse3DI GCAATG 2 cut(s) 645, 1083
Bse8I GATNNNNATC 1 cut(s) 337
BseBI CCWGG 4 cut(s) 495, 581, 737, 1266
BseDI CCNNGG 3 cut(s) 579, 730, 1071
BseGI GGATG 2 cut(s) 58, 322
BseJI GATNNNNATC 1 cut(s) 337
BseLI CCNNNNNNNGG 6 cut(s) 39, 184, 500, 586, 736, 992
BseMI GCAATG 2 cut(s) 645, 1083
BseMII CTCAG 3 cut(s) 127, 455, 638
BseNI ACTGG 2 cut(s) 649, 703
BseRI GAGGAG 1 cut(s) 471
BseSI GKGCMC 1 cut(s) 727
BseXI GCAGC 6 cut(s) 184, 1242, 1245, 1248, 1251, 1254
BseYI CCCAGC 1 cut(s) 1226
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 5 cut(s) 300, 451, 622, 735, 1142
BsiSI CCGG 2 cut(s) 1160, 1194
BslFI GGGAC 3 cut(s) 334, 516, 976
BslI CCNNNNNNNGG 6 cut(s) 39, 184, 500, 586, 736, 992
BsmAI GTCTC 4 cut(s) 397, 478, 987, 1106
BsmFI GGGAC 3 cut(s) 334, 516, 976
BsnI GGCC 5 cut(s) 300, 451, 622, 735, 1142
Bso31I GGTCTC 1 cut(s) 1106
Bsp1286I GDGCHC 1 cut(s) 727
Bsp143I GATC 5 cut(s) 16, 65, 289, 332, 745
BspACI CCGC 2 cut(s) 270, 520
BspANI GGCC 5 cut(s) 300, 451, 622, 735, 1142
BspCNI CTCAG 3 cut(s) 126, 454, 637
BspHI TCATGA 2 cut(s) 226, 1006
BspLI GGNNCC 2 cut(s) 964, 1164
BspPI GGATC 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 1106
BsrDI GCAATG 2 cut(s) 645, 1083
BsrFI RCCGGY 1 cut(s) 1159
BsrI ACTGG 2 cut(s) 649, 703
BssAI RCCGGY 1 cut(s) 1159
BssECI CCNNGG 3 cut(s) 579, 730, 1071
BssMI GATC 5 cut(s) 16, 65, 289, 332, 745
BssT1I CCWWGG 1 cut(s) 1071
Bst2UI CCWGG 4 cut(s) 495, 581, 737, 1266
Bst4CI ACNGT 5 cut(s) 80, 204, 390, 755, 981
Bst6I CTCTTC 2 cut(s) 459, 481
BstAPI GCANNNNNTGC 2 cut(s) 206, 264
BstC8I GCNNGC 3 cut(s) 302, 727, 913
BstDEI CTNAG 5 cut(s) 113, 441, 624, 905, 957
BstF5I GGATG 2 cut(s) 58, 322
BstHHI GCGC 2 cut(s) 269, 911
BstKTI GATC 5 cut(s) 19, 68, 292, 335, 748
BstMAI GTCTC 4 cut(s) 397, 478, 987, 1106
BstMBI GATC 5 cut(s) 16, 65, 289, 332, 745
BstMWI GCNNNNNNNGC 9 cut(s) 189, 206, 264, 932, 1139, 1236, 1239, 1242, 1251
BstNI CCWGG 4 cut(s) 495, 581, 737, 1266
BstSCI CCNGG 4 cut(s) 493, 579, 735, 1264
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 1 cut(s) 727
BstV1I GCAGC 6 cut(s) 184, 1242, 1245, 1248, 1251, 1254
BsuRI GGCC 5 cut(s) 300, 451, 622, 735, 1142
BtsCI GGATG 2 cut(s) 58, 322
BtsIMutI CAGTG 1 cut(s) 1269
Cac8I GCNNGC 3 cut(s) 302, 727, 913
CaiI CAGNNNCTG 1 cut(s) 514
CciI TCATGA 2 cut(s) 226, 1006
CfoI GCGC 2 cut(s) 269, 911
Cfr10I RCCGGY 1 cut(s) 1159
Cfr13I GGNCC 4 cut(s) 298, 620, 701, 963
Csp6I GTAC 3 cut(s) 696, 818, 1043
CviAII CATG 8 cut(s) 39, 184, 193, 227, 325, 1007, 1108, 1293
CviQI GTAC 3 cut(s) 696, 818, 1043
DdeI CTNAG 5 cut(s) 113, 441, 624, 905, 957
DpnI GATC 5 cut(s) 18, 67, 291, 334, 747
DpnII GATC 5 cut(s) 16, 65, 289, 332, 745
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 2 cut(s) 459, 481
EarI CTCTTC 2 cut(s) 459, 481
Eco130I CCWWGG 1 cut(s) 1071
Eco31I GGTCTC 1 cut(s) 1106
Eco47I GGWCC 2 cut(s) 701, 963
Eco57I CTGAAG 1 cut(s) 869
EcoRII CCWGG 4 cut(s) 493, 579, 735, 1264
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 8 cut(s) 42, 187, 196, 230, 328, 1010, 1111, 1296
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 3 cut(s) 334, 516, 976
FatI CATG 8 cut(s) 38, 183, 192, 226, 324, 1006, 1107, 1292
FbaI TGATCA 2 cut(s) 16, 289
Fnu4HI GCNGC 8 cut(s) 198, 270, 520, 1231, 1234, 1237, 1240, 1243
FokI GGATG 2 cut(s) 65, 329
Fsp4HI GCNGC 8 cut(s) 198, 270, 520, 1231, 1234, 1237, 1240, 1243
FspBI CTAG 2 cut(s) 353, 705
GlaI GCGC 2 cut(s) 268, 910
GluI GCNGC 8 cut(s) 198, 270, 520, 1231, 1234, 1237, 1240, 1243
GsaI CCCAGC 1 cut(s) 1230
HaeIII GGCC 5 cut(s) 300, 451, 622, 735, 1142
HapII CCGG 2 cut(s) 1160, 1194
HhaI GCGC 2 cut(s) 269, 911
Hin1II CATG 8 cut(s) 42, 187, 196, 230, 328, 1010, 1111, 1296
Hin6I GCGC 2 cut(s) 267, 909
HinP1I GCGC 2 cut(s) 267, 909
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 7 cut(s) 10, 236, 405, 628, 802, 1151, 1169
HpaII CCGG 2 cut(s) 1160, 1194
HphI GGTGA 2 cut(s) 553, 1149
Hpy166II GTNNAC 3 cut(s) 119, 386, 1219
Hpy188I TCNGA 6 cut(s) 165, 241, 402, 553, 627, 774
Hpy188III TCNNGA 5 cut(s) 14, 227, 280, 1007, 1166
Hpy8I GTNNAC 3 cut(s) 119, 386, 1219
Hpy99I CGWCG 1 cut(s) 1121
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 5 cut(s) 80, 204, 390, 755, 981
HpyCH4V TGCA 8 cut(s) 200, 245, 258, 304, 652, 808, 1081, 1107
HpyF10VI GCNNNNNNNGC 9 cut(s) 189, 206, 264, 932, 1139, 1236, 1239, 1242, 1251
HpyF3I CTNAG 5 cut(s) 113, 441, 624, 905, 957
Hsp92II CATG 8 cut(s) 42, 187, 196, 230, 328, 1010, 1111, 1296
HspAI GCGC 2 cut(s) 267, 909
Ksp22I TGATCA 2 cut(s) 16, 289
Kzo9I GATC 5 cut(s) 16, 65, 289, 332, 745
LmnI GCTCC 2 cut(s) 923, 1168
Lsp1109I GCAGC 6 cut(s) 184, 1242, 1245, 1248, 1251, 1254
LweI GCATC 3 cut(s) 232, 854, 1101
MaeI CTAG 2 cut(s) 353, 705
MaeIII GTNAC 5 cut(s) 80, 320, 559, 1066, 1247
MalI GATC 5 cut(s) 18, 67, 291, 334, 747
MboI GATC 5 cut(s) 16, 65, 289, 332, 745
MboII GAAGA 6 cut(s) 146, 446, 468, 811, 875, 1198
MfeI CAATTG 3 cut(s) 253, 262, 376
MhlI GDGCHC 1 cut(s) 727
MlyI GAGTC 2 cut(s) 245, 399
Mph1103I ATGCAT 1 cut(s) 1083
MroXI GAANNNNTTC 2 cut(s) 108, 768
MseI TTAA 1 cut(s) 285
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 2 cut(s) 1160, 1194
MspR9I CCNGG 4 cut(s) 495, 581, 737, 1266
MunI CAATTG 3 cut(s) 253, 262, 376
MvaI CCWGG 4 cut(s) 495, 581, 737, 1266
MwoI GCNNNNNNNGC 9 cut(s) 189, 206, 264, 932, 1139, 1236, 1239, 1242, 1251
NdeII GATC 5 cut(s) 16, 65, 289, 332, 745
NlaIII CATG 8 cut(s) 42, 187, 196, 230, 328, 1010, 1111, 1296
NlaIV GGNNCC 2 cut(s) 964, 1164
NmeAIII GCCGAG 2 cut(s) 755, 1201
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 1083
PagI TCATGA 2 cut(s) 226, 1006
PdmI GAANNNNTTC 2 cut(s) 108, 768
PfeI GAWTC 5 cut(s) 10, 628, 802, 1151, 1169
PflMI CCANNNNNTGG 3 cut(s) 184, 586, 992
PkrI GCNGC 8 cut(s) 199, 271, 521, 1232, 1235, 1238, 1241, 1244
PleI GAGTC 2 cut(s) 244, 399
PpsI GAGTC 2 cut(s) 244, 399
PshBI ATTAAT 1 cut(s) 285
Psp6I CCWGG 4 cut(s) 493, 579, 735, 1264
PspFI CCCAGC 1 cut(s) 1226
PspGI CCWGG 4 cut(s) 493, 579, 735, 1264
PspN4I GGNNCC 2 cut(s) 964, 1164
PspPI GGNCC 4 cut(s) 298, 620, 701, 963
PstNI CAGNNNCTG 1 cut(s) 514
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 3 cut(s) 697, 819, 1044
RsaNI GTAC 3 cut(s) 696, 818, 1043
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 8 cut(s) 198, 270, 520, 1231, 1234, 1237, 1240, 1243
Sau3AI GATC 5 cut(s) 16, 65, 289, 332, 745
Sau96I GGNCC 4 cut(s) 298, 620, 701, 963
ScaI AGTACT 1 cut(s) 1044
SchI GAGTC 2 cut(s) 245, 399
ScrFI CCNGG 4 cut(s) 495, 581, 737, 1266
SduI GDGCHC 1 cut(s) 727
SetI ASST 9 cut(s) 447, 463, 516, 706, 903, 928, 1006, 1102, 1262
SfaNI GCATC 3 cut(s) 232, 854, 1101
SfcI CTRYAG 1 cut(s) 927
SinI GGWCC 2 cut(s) 701, 963
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SsiI CCGC 2 cut(s) 270, 520
SspMI CTAG 2 cut(s) 353, 705
StyD4I CCNGG 4 cut(s) 493, 579, 735, 1264
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 5 cut(s) 80, 204, 390, 755, 981
TaqI TCGA 2 cut(s) 281, 867
TatI WGTACW 2 cut(s) 695, 1042
TauI GCSGC 2 cut(s) 272, 522
TfiI GAWTC 5 cut(s) 10, 628, 802, 1151, 1169
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 1276
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 6 cut(s) 197, 1230, 1233, 1236, 1239, 1242
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 204, 812, 873, 1038
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 1 cut(s) 1276
Van91I CCANNNNNTGG 3 cut(s) 184, 586, 992
VpaK11BI GGWCC 2 cut(s) 701, 963
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 1 cut(s) 1180
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XmnI GAANNNNTTC 2 cut(s) 108, 768
XspI CTAG 2 cut(s) 353, 705
ZrmI AGTACT 1 cut(s) 1044
Zsp2I ATGCAT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.