MD02G1123800.v1.1

NEP1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006207
Physical Location & Seq
Forward (+)
10088663 .. 10091466
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1123800.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGATTTTATTGCGCACCCATCTCATTTGTCTCCTTCCTATTCGTTTTCGTTGGGGAATTTCGTCGAACGGGTCAAGGATTTCTGCAACTTCGCCGTCTCTGTGGTTCTCGGGAACGTTTTCTCTGCGATCTTCACCTTCTTCTTTGCATTAGTGGGCAGCACGTTAGGAGCAATGACTGGAGCTTTGATAGGGCAAGAAACTGAGAGTGGATTTGTGAGAGGAGCTGCAGTTGGAGCAATATCAGGAGCTGTTTTTTCAATTGAAGTGTTTGAATCTTCCCTTGTTCTTTGGCAATCAGATGAATCAGGAATTGGGTGTCTTCTGTATTTGATTGATGTCATTGCTAGTCTCCTAAGCGGTAGATTAGTCCGCGAGCGGATTGGCCCAGCCATGTTAAGTGCAGTGCAAAGTCAGATGGGTGCTGTGGAATCAACCTTTGATGAGATCCCCAACATCTTCGACACCGGCAGCTCCAAAGGGTTGCCCGGAGACTCAGTTGAAAAGATCCCGAAGATCAAAATTACCTGCCACAACAATGTGGATGCTTCGGGGGAGAAAGTCTCCTGTTCAGTTTGTCTTCAGGATTTTCAGATTGGAGAGACAGTAAGAAGCTTGCCACACTGCCATCACATGTTTCACCTGCCTTGTATAGATAAGTGGCTCCTAAGCCATGGCTCATGCCCTCTCTGCAGAAGGGATCTGTGA

Protein Analysis

236

Amino Acids

25.28

Weight (kDa)

5.4

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-zipper_YMGG PF13441 50 - 85 4.2e-06 YMGG-like Gly-zipper
zf-RING_2 PF13639 189 - 232 4.5e-13 Ring finger domain
zf-RING_11 PF17123 190 - 218 2.5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 190 - 231 5.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 203 - 232 2.9e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 651
Acc16I TGCGCA 1 cut(s) 15
Acc36I ACCTGC 2 cut(s) 536, 651
AccBSI CCGCTC 1 cut(s) 379
AccII CGCG 1 cut(s) 375
AciI CCGC 3 cut(s) 360, 373, 379
AclI AACGTT 1 cut(s) 117
AclWI GGATC 2 cut(s) 442, 502
AcsI RAATTY 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 566
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 4 cut(s) 261, 266, 275, 503
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 5 cut(s) 185, 227, 251, 474, 615
AluI AGCT 5 cut(s) 185, 227, 251, 474, 615
Alw26I GTCTC 6 cut(s) 36, 103, 356, 486, 568, 596
AlwI GGATC 2 cut(s) 442, 502
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 159, 227, 471
ApoI RAATTY 1 cut(s) 58
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
Asp700I GAANNNNTTC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 489
AsuHPI GGTGA 2 cut(s) 127, 632
AvaI CYCGRG 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 314, 572
BbvI GCAGC 3 cut(s) 171, 214, 483
BccI CCATC 3 cut(s) 28, 412, 636
BceAI ACGGC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 489
BcoDI GTCTC 6 cut(s) 36, 103, 356, 486, 568, 596
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 2 cut(s) 228, 691
BfuAI ACCTGC 2 cut(s) 536, 651
BisI GCNGC 3 cut(s) 160, 228, 472
BlsI GCNGC 3 cut(s) 161, 229, 473
Bme1390I CCNGG 1 cut(s) 489
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 665
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 535
BpiI GAAGAC 2 cut(s) 314, 572
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 2 cut(s) 356, 668
BpuMI CCSGG 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 673
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 483, 513, 591, 621
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 2 cut(s) 180, 342
BseDI CCNNGG 1 cut(s) 673
BseGI GGATG 1 cut(s) 550
BseMI GCAATG 2 cut(s) 180, 342
BseMII CTCAG 2 cut(s) 195, 510
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 3 cut(s) 171, 214, 483
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 468, 489
BsmAI GTCTC 6 cut(s) 36, 103, 356, 486, 568, 596
BsmBI CGTCTC 1 cut(s) 103
BsnI GGCC 1 cut(s) 387
BsoBI CYCGRG 1 cut(s) 110
Bsp143I GATC 5 cut(s) 129, 447, 507, 516, 700
Bsp19I CCATGG 1 cut(s) 673
BspACI CCGC 3 cut(s) 360, 373, 379
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 2 cut(s) 196, 509
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 665
BspMAI CTGCAG 2 cut(s) 232, 695
BspMI ACCTGC 2 cut(s) 536, 651
BspPI GGATC 2 cut(s) 442, 502
BsrBI CCGCTC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 180, 342
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 1 cut(s) 673
BssMI GATC 5 cut(s) 129, 447, 507, 516, 700
BssT1I CCWWGG 1 cut(s) 673
Bst4CI ACNGT 1 cut(s) 607
BstC8I GCNNGC 2 cut(s) 377, 617
BstDEI CTNAG 4 cut(s) 204, 356, 496, 668
BstDSI CCRYGG 1 cut(s) 673
BstF5I GGATG 1 cut(s) 550
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 5 cut(s) 132, 450, 510, 519, 703
BstMAI GTCTC 6 cut(s) 36, 103, 356, 486, 568, 596
BstMBI GATC 5 cut(s) 129, 447, 507, 516, 700
BstMWI GCNNNNNNNGC 2 cut(s) 236, 690
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 2 cut(s) 228, 691
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 3 cut(s) 171, 214, 483
BstV2I GAAGAC 2 cut(s) 314, 572
BstX2I RGATCY 3 cut(s) 447, 507, 700
BstYI RGATCY 3 cut(s) 447, 507, 700
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 673
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 2 cut(s) 411, 622
BtsIMutI CAGTG 2 cut(s) 411, 622
BveI ACCTGC 2 cut(s) 536, 651
Cac8I GCNNGC 2 cut(s) 377, 617
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 386
CviAII CATG 4 cut(s) 394, 634, 674, 681
DdeI CTNAG 4 cut(s) 204, 356, 496, 668
DpnI GATC 5 cut(s) 131, 449, 509, 518, 702
DpnII GATC 5 cut(s) 129, 447, 507, 516, 700
Eco130I CCWWGG 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 673
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 4 cut(s) 397, 637, 677, 684
FaiI YATR 5 cut(s) 395, 635, 653, 675, 682
FatI CATG 4 cut(s) 393, 633, 673, 680
Fnu4HI GCNGC 3 cut(s) 160, 228, 472
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 3 cut(s) 160, 228, 472
FspBI CTAG 1 cut(s) 348
FspI TGCGCA 1 cut(s) 15
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 160, 228, 472
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 201
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 468, 489
HhaI GCGC 1 cut(s) 16
Hin1II CATG 4 cut(s) 397, 637, 677, 684
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 4 cut(s) 275, 305, 431, 494
HpaII CCGG 2 cut(s) 468, 489
HphI GGTGA 2 cut(s) 127, 632
Hpy188I TCNGA 3 cut(s) 301, 417, 594
Hpy188III TCNNGA 5 cut(s) 112, 246, 309, 511, 584
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 45, 148, 690
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 2 cut(s) 117, 164
HpyCH4V TGCA 6 cut(s) 87, 149, 230, 404, 409, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 690
HpyF3I CTNAG 4 cut(s) 204, 356, 496, 668
HpySE526I ACGT 2 cut(s) 117, 164
Hsp92II CATG 4 cut(s) 397, 637, 677, 684
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 5 cut(s) 129, 447, 507, 516, 700
LmnI GCTCC 7 cut(s) 170, 182, 224, 236, 248, 479, 669
Lsp1109I GCAGC 3 cut(s) 171, 214, 483
LweI GCATC 1 cut(s) 535
MaeI CTAG 1 cut(s) 348
MaeII ACGT 2 cut(s) 117, 164
MalI GATC 5 cut(s) 131, 449, 509, 518, 702
MbiI CCGCTC 1 cut(s) 379
MboI GATC 5 cut(s) 129, 447, 507, 516, 700
MboII GAAGA 7 cut(s) 124, 133, 270, 314, 451, 526, 572
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 3 cut(s) 447, 507, 700
MluCI AATT 4 cut(s) 58, 261, 312, 522
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 2 cut(s) 215, 696
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 1 cut(s) 537
MspI CCGG 2 cut(s) 468, 489
MspR9I CCNGG 1 cut(s) 489
MunI CAATTG 1 cut(s) 261
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 2 cut(s) 236, 690
NciI CCSGG 1 cut(s) 489
NcoI CCATGG 1 cut(s) 673
NdeII GATC 5 cut(s) 129, 447, 507, 516, 700
NlaIII CATG 4 cut(s) 397, 637, 677, 684
NlaIV GGNNCC 1 cut(s) 665
NsbI TGCGCA 1 cut(s) 15
NspI RCATGY 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 651
PciI ACATGT 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 275, 305, 431
PkrI GCNGC 3 cut(s) 161, 229, 473
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PscI ACATGT 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 388
PspN4I GGNNCC 1 cut(s) 665
PspPI GGNCC 1 cut(s) 386
PstI CTGCAG 2 cut(s) 232, 695
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 3 cut(s) 447, 507, 700
RseI CAYNNNNRTG 1 cut(s) 537
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 3 cut(s) 160, 228, 472
Sau3AI GATC 5 cut(s) 129, 447, 507, 516, 700
Sau96I GGNCC 1 cut(s) 386
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 489
SfaNI GCATC 1 cut(s) 535
SfcI CTRYAG 2 cut(s) 228, 691
SmiMI CAYNNNNRTG 1 cut(s) 537
Sse9I AATT 4 cut(s) 58, 261, 312, 522
SsiI CCGC 3 cut(s) 360, 373, 379
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 487
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 2 cut(s) 120, 167
TaqI TCGA 2 cut(s) 66, 462
TasI AATT 4 cut(s) 58, 261, 312, 522
TfiI GAWTC 3 cut(s) 275, 305, 431
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 2 cut(s) 411, 629
TseI GCWGC 3 cut(s) 159, 227, 471
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 2 cut(s) 411, 629
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.