Rh2AG119900

NEP1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006207
Physical Location & Seq
Forward (+)
10762945 .. 10764524
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG119900.1

Sequence Viewer

Length: 708 bp
ATGGATTTTATAGCACACCCATCTCGTTTCTCGTCCTCCTTTTCCTATTCCTTTGGAAATATCTTCGAGAGGGTTAAAGATTTTTGCAACTTTGCGGTTTCTGCGATTATTGGGAACGTTTTCTCTGCGATCTTCACCTTCTTCTTTGCTTTGGTGGGCACCTTGTTGGGAGCTATGACCGGAGCTTTGATAGGACAAGAAACCGAGAGCGGATTTGTTAGGGGTGCTGCTGTTGGAGCAATCTCAGGAGCTGTTTTCTCCATTGAAGTGTTTGAATCATCCCTTATTCTATGGCAATCAGATGAATCCGGAATTGGGTGTCTTCTCTACTTGATTGATGTCATTGCGAGTCTCCTAAGTGGTAGATTAGTACGCGAGCGTATCGGTCCAGCCATGTTGAGTGCAGTGCAAAGTCAGATGGTTGCTGTTGAATCAAGCTTTGAAGAGATTCCTAACATCTTTGACACCGGTGGGGCCAAAGGATTGCCTGGAGATTCAGTTGAAAAGATCCCAAAGATCATCATCACTAGCAACAATAATGTGGATGCTTCTGGAGATAAAGTCTCATGTTCAGTGTGCCTTCAGGACTTTCAGCTTGGAGAGACAGTCAGAAGTTTACCACAATGCCATCACATGTTTCACCTGCCATGTATAGATAAGTGGCTCCTGAGGCATGGCTCTTGCCCACTTTGCAGAAGGGATCTGTGA

Protein Analysis

235

Amino Acids

25.39

Weight (kDa)

5.17

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-zipper_YMGG PF13441 50 - 85 2.9e-07 YMGG-like Gly-zipper
zf-RING_2 PF13639 189 - 232 2.8e-13 Ring finger domain
zf-RING_11 PF17123 190 - 218 6.9e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 190 - 231 1.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 204 - 232 8.5e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 651
Acc36I ACCTGC 1 cut(s) 651
AccB1I GGYRCC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 210
AccII CGCG 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 308
AciI CCGC 2 cut(s) 95, 210
AclI AACGTT 1 cut(s) 117
AclWI GGATC 1 cut(s) 502
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 1 cut(s) 315
AflIII ACRYGT 1 cut(s) 633
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 5 cut(s) 266, 275, 431, 443, 503
AjnI CCWGG 1 cut(s) 487
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 5 cut(s) 173, 185, 251, 438, 595
AluI AGCT 5 cut(s) 173, 185, 251, 438, 595
Alw26I GTCTC 3 cut(s) 356, 568, 596
AlwI GGATC 1 cut(s) 502
AlwNI CAGNNNCTG 1 cut(s) 251
Aor13HI TCCGGA 1 cut(s) 308
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 227
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AsiGI ACCGGT 1 cut(s) 467
Asp700I GAANNNNTTC 2 cut(s) 119, 447
AspS9I GGNCC 2 cut(s) 386, 474
AsuHPI GGTGA 2 cut(s) 127, 632
AvaII GGWCC 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 1 cut(s) 214
BccI CCATC 3 cut(s) 28, 412, 636
BciT130I CCWGG 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 356, 568, 596
BfaI CTAG 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 651
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 386, 474
BmiI GGNNCC 3 cut(s) 160, 475, 665
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 2 cut(s) 510, 573
BsaBI GATNNNNATC 1 cut(s) 521
BsaWI WCCGGW 3 cut(s) 179, 308, 467
BsaXI ACNNNNNCTCC 6 cut(s) 483, 513, 546, 576, 591, 621
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 467
Bse21I CCTNAGG 1 cut(s) 668
Bse3DI GCAATG 1 cut(s) 342
Bse8I GATNNNNATC 1 cut(s) 521
BseAI TCCGGA 1 cut(s) 308
BseBI CCWGG 1 cut(s) 489
BseGI GGATG 2 cut(s) 278, 550
BseJI GATNNNNATC 1 cut(s) 521
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 258, 659
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 476
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 467
BsiSI CCGG 3 cut(s) 180, 309, 468
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 3 cut(s) 356, 568, 596
BsnI GGCC 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 161
Bsp13I TCCGGA 1 cut(s) 308
Bsp143I GATC 4 cut(s) 129, 507, 516, 700
BspACI CCGC 2 cut(s) 95, 210
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 2 cut(s) 257, 660
BspEI TCCGGA 1 cut(s) 308
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 160, 475, 665
BspMI ACCTGC 1 cut(s) 651
BspPI GGATC 1 cut(s) 502
BspT107I GGYRCC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 467
BssMI GATC 4 cut(s) 129, 507, 516, 700
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 3 cut(s) 244, 356, 668
BstF5I GGATG 2 cut(s) 278, 550
BstFNI CGCG 1 cut(s) 375
BstKTI GATC 4 cut(s) 132, 510, 519, 703
BstMAI GTCTC 3 cut(s) 356, 568, 596
BstMBI GATC 4 cut(s) 129, 507, 516, 700
BstMWI GCNNNNNNNGC 4 cut(s) 101, 236, 670, 690
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 1 cut(s) 487
BstSLI GKGCMC 1 cut(s) 161
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 2 cut(s) 507, 700
BstYI RGATCY 2 cut(s) 507, 700
Bsu36I CCTNAGG 1 cut(s) 668
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 2 cut(s) 278, 550
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 411, 579
BveI ACCTGC 1 cut(s) 651
Cac8I GCNNGC 1 cut(s) 377
CaiI CAGNNNCTG 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 386, 474
Csp6I GTAC 1 cut(s) 371
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 5 cut(s) 394, 567, 634, 648, 674
CviJI RGCY 9 cut(s) 173, 185, 251, 392, 438, 476, 595, 664, 678
CviKI_1 RGCY 9 cut(s) 173, 185, 251, 392, 438, 476, 595, 664, 678
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 3 cut(s) 244, 356, 668
DpnI GATC 4 cut(s) 131, 509, 518, 702
DpnII GATC 4 cut(s) 129, 507, 516, 700
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 566
Eco81I CCTNAGG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 5 cut(s) 397, 570, 637, 651, 677
FaiI YATR 9 cut(s) 11, 176, 292, 395, 568, 635, 649, 653, 675
FatI CATG 5 cut(s) 393, 566, 633, 647, 673
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 2 cut(s) 265, 557
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 1 cut(s) 528
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 2 cut(s) 510, 573
HaeIII GGCC 1 cut(s) 476
HapII CCGG 3 cut(s) 180, 309, 468
Hin1II CATG 5 cut(s) 397, 570, 637, 651, 677
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 6 cut(s) 275, 305, 349, 431, 448, 494
HpaII CCGG 3 cut(s) 180, 309, 468
HphI GGTGA 2 cut(s) 127, 632
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 3 cut(s) 301, 417, 611
Hpy188III TCNNGA 6 cut(s) 67, 246, 309, 552, 584, 667
Hpy8I GTNNAC 1 cut(s) 617
HpyAV CCTTC 3 cut(s) 148, 590, 690
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 87, 404, 409, 693
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 236, 670, 690
HpyF3I CTNAG 3 cut(s) 244, 356, 668
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 5 cut(s) 397, 570, 637, 651, 677
Kpn2I TCCGGA 1 cut(s) 308
Kzo9I GATC 4 cut(s) 129, 507, 516, 700
LmnI GCTCC 5 cut(s) 170, 182, 236, 248, 669
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 535
MaeI CTAG 1 cut(s) 528
MaeII ACGT 1 cut(s) 117
MalI GATC 4 cut(s) 131, 509, 518, 702
MbiI CCGCTC 1 cut(s) 210
MboI GATC 4 cut(s) 129, 507, 516, 700
MboII GAAGA 5 cut(s) 55, 124, 133, 314, 455
MflI RGATCY 2 cut(s) 507, 700
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 1 cut(s) 312
MlyI GAGTC 1 cut(s) 358
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 3 cut(s) 46, 63, 663
MroI TCCGGA 1 cut(s) 308
MroXI GAANNNNTTC 2 cut(s) 119, 447
MseI TTAA 1 cut(s) 75
MslI CAYNNNNRTG 1 cut(s) 266
MspI CCGG 3 cut(s) 180, 309, 468
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 4 cut(s) 101, 236, 670, 690
NdeII GATC 4 cut(s) 129, 507, 516, 700
NlaIII CATG 5 cut(s) 397, 570, 637, 651, 677
NlaIV GGNNCC 3 cut(s) 160, 475, 665
NspI RCATGY 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 651
PciI ACATGT 1 cut(s) 633
PdmI GAANNNNTTC 2 cut(s) 119, 447
PfeI GAWTC 5 cut(s) 275, 305, 431, 448, 494
PinAI ACCGGT 1 cut(s) 467
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 357
PpsI GAGTC 1 cut(s) 357
PscI ACATGT 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 3 cut(s) 160, 475, 665
PspPI GGNCC 2 cut(s) 386, 474
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 2 cut(s) 507, 700
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 4 cut(s) 129, 507, 516, 700
Sau96I GGNCC 2 cut(s) 386, 474
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 161
SetI ASST 9 cut(s) 120, 140, 164, 175, 187, 253, 440, 597, 645
SfaNI GCATC 1 cut(s) 535
SgrAI CRCCGGYG 1 cut(s) 467
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 1 cut(s) 312
SsiI CCGC 2 cut(s) 95, 210
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 66
TaqII GACCGA 1 cut(s) 374
TasI AATT 1 cut(s) 312
TfiI GAWTC 5 cut(s) 275, 305, 431, 448, 494
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 411, 579
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 2 cut(s) 411, 579
VpaK11BI GGWCC 1 cut(s) 386
XceI RCATGY 1 cut(s) 637
XmnI GAANNNNTTC 2 cut(s) 119, 447
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.