Rh2DG124700

NEP1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006207
Physical Location & Seq
Forward (+)
10373999 .. 10378170
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG124700.1

Sequence Viewer

Length: 573 bp
ATGACATTTTTGTGTGCAGTGGGCACCTTGTTGGGAGCTATGACCGGAGCTTTGATAGGACAAGAAACCGAGAGCGGATTTGTTAGGGGTGCTGCTGTTGGAGCAATCTCAGGAGCTGTTTTCTCCATTGAAGTGTTTGAATCATCCCTTATTCTATGGCAATCAGATGAATCCGGAATTGGGTGTCTTCTCTACTTGATTGATGTCATTGCGAGTCTCCTAAGTGGTAGATTAGTCCGCGAGCGAATCGGTCCAGCCATGTTGAGTGCAGTGCAAAGTCAGATGGTTGCTGTTGAATCAAGCTTTGAAGAGATTCCTAACATCTTTGACACCGGTGGGGCCAAAGGATTGCCCGGAGATTCAGTTGAAAAGATCCCAAAGATAATCATCACTGGCAACAATAATGTGGATGCTTCTGGAGATAAAGTCTCATGTTCAGTTTGCCTTCAGGACTTTCAGCTTGGAGAGACAGTCAGAAGTTTACCACAATGCCATCACATGTTTCACCTGCCATGTATAGATAAGTGGCTCCTGAGGCATGGCTCTTGCCCACTTTGCAGAAGGGATCTGTGA

Protein Analysis

190

Amino Acids

20.24

Weight (kDa)

5.0

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-zipper_YMGG PF13441 6 - 40 2.1e-06 YMGG-like Gly-zipper
zf-RING_2 PF13639 144 - 187 2e-13 Ring finger domain
zf-RING_11 PF17123 145 - 173 5e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 145 - 186 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 159 - 187 6.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 516
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 75
AccII CGCG 1 cut(s) 240
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 2 cut(s) 75, 238
AclWI GGATC 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 180
AflIII ACRYGT 1 cut(s) 498
AgeI ACCGGT 1 cut(s) 332
AgsI TTSAA 5 cut(s) 131, 140, 296, 308, 368
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 5 cut(s) 38, 50, 116, 303, 460
AluI AGCT 5 cut(s) 38, 50, 116, 303, 460
Alw26I GTCTC 3 cut(s) 221, 433, 461
AlwI GGATC 1 cut(s) 367
AlwNI CAGNNNCTG 1 cut(s) 116
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AsiGI ACCGGT 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 251, 339
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 497
AvaII GGWCC 1 cut(s) 251
AxyI CCTNAGG 1 cut(s) 533
BaeGI GKGCMC 1 cut(s) 26
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 277, 501
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 3 cut(s) 221, 433, 461
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 251, 339
BmiI GGNNCC 3 cut(s) 25, 340, 530
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 354
BsaBI GATNNNNATC 1 cut(s) 386
BsaWI WCCGGW 3 cut(s) 44, 173, 332
BsaXI ACNNNNNCTCC 6 cut(s) 348, 378, 411, 441, 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 1 cut(s) 397
Bse21I CCTNAGG 1 cut(s) 533
Bse3DI GCAATG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 386
BseAI TCCGGA 1 cut(s) 173
BseGI GGATG 2 cut(s) 143, 415
BseJI GATNNNNATC 1 cut(s) 386
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 123, 524
BseNI ACTGG 1 cut(s) 397
BseSI GKGCMC 1 cut(s) 26
BseXI GCAGC 1 cut(s) 79
BsgI GTGCAG 2 cut(s) 36, 288
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 23
BshTI ACCGGT 1 cut(s) 332
BsiSI CCGG 4 cut(s) 45, 174, 333, 354
BslI CCNNNNNNNGG 1 cut(s) 180
BsmAI GTCTC 3 cut(s) 221, 433, 461
BsnI GGCC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 26
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 2 cut(s) 372, 565
BspACI CCGC 2 cut(s) 75, 238
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 2 cut(s) 122, 525
BspEI TCCGGA 1 cut(s) 173
BspFNI CGCG 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 25, 340, 530
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 23
BsrBI CCGCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 1 cut(s) 332
BssMI GATC 2 cut(s) 372, 565
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 3 cut(s) 109, 221, 533
BstF5I GGATG 2 cut(s) 143, 415
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 2 cut(s) 375, 568
BstMAI GTCTC 3 cut(s) 221, 433, 461
BstMBI GATC 2 cut(s) 372, 565
BstMWI GCNNNNNNNGC 3 cut(s) 101, 535, 555
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 352
BstSLI GKGCMC 1 cut(s) 26
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 2 cut(s) 372, 565
BstYI RGATCY 2 cut(s) 372, 565
Bsu36I CCTNAGG 1 cut(s) 533
BsuRI GGCC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 143, 415
BtsI GCAGTG 2 cut(s) 24, 276
BtsIMutI CAGTG 3 cut(s) 24, 276, 390
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 242
CaiI CAGNNNCTG 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 251, 339
CspAI ACCGGT 1 cut(s) 332
CviAII CATG 5 cut(s) 259, 432, 499, 513, 539
CviJI RGCY 9 cut(s) 38, 50, 116, 257, 303, 341, 460, 529, 543
CviKI_1 RGCY 9 cut(s) 38, 50, 116, 257, 303, 341, 460, 529, 543
DdeI CTNAG 3 cut(s) 109, 221, 533
DpnI GATC 2 cut(s) 374, 567
DpnII GATC 2 cut(s) 372, 565
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 533
FaeI CATG 5 cut(s) 262, 435, 502, 516, 542
FaiI YATR 8 cut(s) 41, 157, 260, 433, 500, 514, 518, 540
FatI CATG 5 cut(s) 258, 431, 498, 512, 538
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 2 cut(s) 130, 422
Fsp4HI GCNGC 1 cut(s) 93
GluI GCNGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 341
HapII CCGG 4 cut(s) 45, 174, 333, 354
Hin1II CATG 5 cut(s) 262, 435, 502, 516, 542
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 7 cut(s) 140, 170, 214, 246, 296, 313, 359
HpaII CCGG 4 cut(s) 45, 174, 333, 354
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 1 cut(s) 482
Hpy188I TCNGA 3 cut(s) 166, 282, 476
Hpy188III TCNNGA 5 cut(s) 111, 174, 417, 449, 532
Hpy8I GTNNAC 1 cut(s) 482
HpyAV CCTTC 2 cut(s) 455, 555
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4V TGCA 4 cut(s) 17, 269, 274, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 535, 555
HpyF3I CTNAG 3 cut(s) 109, 221, 533
Hsp92II CATG 5 cut(s) 262, 435, 502, 516, 542
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 2 cut(s) 372, 565
LmnI GCTCC 5 cut(s) 35, 47, 101, 113, 534
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 400
MalI GATC 2 cut(s) 374, 567
MbiI CCGCTC 1 cut(s) 75
MboI GATC 2 cut(s) 372, 565
MboII GAAGA 2 cut(s) 179, 320
MflI RGATCY 2 cut(s) 372, 565
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 1 cut(s) 177
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 1 cut(s) 528
MroI TCCGGA 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 312
MslI CAYNNNNRTG 2 cut(s) 10, 131
MspI CCGG 4 cut(s) 45, 174, 333, 354
MspR9I CCNGG 1 cut(s) 354
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 101, 535, 555
NciI CCSGG 1 cut(s) 354
NdeII GATC 2 cut(s) 372, 565
NlaIII CATG 5 cut(s) 262, 435, 502, 516, 542
NlaIV GGNNCC 3 cut(s) 25, 340, 530
NspI RCATGY 1 cut(s) 502
PaqCI CACCTGC 1 cut(s) 516
PciI ACATGT 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 6 cut(s) 140, 170, 246, 296, 313, 359
PinAI ACCGGT 1 cut(s) 332
PkrI GCNGC 1 cut(s) 94
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PscI ACATGT 1 cut(s) 498
PspN4I GGNNCC 3 cut(s) 25, 340, 530
PspPI GGNCC 2 cut(s) 251, 339
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 2 cut(s) 372, 565
RseI CAYNNNNRTG 2 cut(s) 10, 131
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 372, 565
Sau96I GGNCC 2 cut(s) 251, 339
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 26
SetI ASST 7 cut(s) 29, 40, 52, 118, 305, 462, 510
SfaNI GCATC 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 332
SinI GGWCC 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 10, 131
Sse9I AATT 1 cut(s) 177
SsiI CCGC 2 cut(s) 75, 238
StyD4I CCNGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 472
TaqII GACCGA 1 cut(s) 239
TasI AATT 1 cut(s) 177
TfiI GAWTC 6 cut(s) 140, 170, 246, 296, 313, 359
TscAI CASTG 3 cut(s) 24, 276, 397
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 3 cut(s) 24, 276, 397
VpaK11BI GGWCC 1 cut(s) 251
XceI RCATGY 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.