MD15G1237800.v1.1

NEP1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006207
Physical Location & Seq
Forward (+)
19565203 .. 19567150
1948 bp
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UTR
Exon/CDS
Intron
MD15G1237800.v1.1.491

Sequence Viewer

Length: 714 bp
ATGGCAATGGATTTTATTGCGCACCCATCTCGTTTTTCTCCTTCCTATTCGTTTTCGTTGGGGAATTTCATCGAACGGGTCAAGGATTTCTGCAACTTCGCCGTCTCTGCGGTTCTGGGGAACGTTTTCTCTGCGATCTTCACCTTCTTCTTTGCATTAGTGGGCACCATGTTAGGAGCAATGACTGGAGCTTTGATAGGGCAAGAAACTGAGAGTGGATTTGTGAGAGGAGCTGCAGTTGGAGCAATATCAGGAGCTGTTTTTTCAATTGAAGTGTTTGAATCTTCCCTTATTCTTTGGCAATCAGATGAATCAGGAATTGGGTGTCTTCTGTATTTGATTGATGTCATTGCTAGTCTCCTAAGCGGTAGATTAGTCCGCGAGCGGATTGGCCCCGCCATGTTAAGTGCAGTGCAAAGTCAGATGGGTGCTGTGGAATCAAGCTTTGACGAGATCCCCAACATCTTCGACACTGGCGGCTCCAAAGGGTTGCCCGGAGATTCAGTTGAAAAGATCCCAAAGATCAAAATTACCTGCCACAACAACGTGGATGCTTCTGGGGAGAAAGTCTCCTGTTCAGTTTGCCTTCAGGATTTTCAGCTTGGAGAGACAGTAAGAAGTTTGCCACACTGCCATCACATGTTTCACTTGCCTTGTATAGATAAGTGGCTCCTAAGGCATGGCTCATGCCCTCTCTGCAGAAGGGATCTGTGA

Protein Analysis

238

Amino Acids

25.61

Weight (kDa)

5.6

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-zipper_YMGG PF13441 52 - 87 3.5e-07 YMGG-like Gly-zipper
zf-RING_2 PF13639 191 - 234 6.3e-13 Ring finger domain
zf-RING_11 PF17123 192 - 220 2.8e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 192 - 233 6.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 205 - 234 2.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 385
AccII CGCG 1 cut(s) 381
AciI CCGC 6 cut(s) 110, 366, 379, 385, 396, 477
AclI AACGTT 1 cut(s) 123
AclWI GGATC 2 cut(s) 448, 508
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 4 cut(s) 267, 272, 281, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
AluBI AGCT 5 cut(s) 191, 233, 257, 444, 601
AluI AGCT 5 cut(s) 191, 233, 257, 444, 601
Alw26I GTCTC 4 cut(s) 109, 362, 574, 602
AlwI GGATC 2 cut(s) 448, 508
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 64
ArsI GACNNNNNNTTYG 2 cut(s) 175, 207
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 392
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 1 cut(s) 133
AxyI CCTNAGG 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 220
BccI CCATC 3 cut(s) 34, 418, 642
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 4 cut(s) 109, 362, 574, 602
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 2 cut(s) 234, 697
BfuAI ACCTGC 1 cut(s) 542
BisI GCNGC 2 cut(s) 234, 478
BlsI GCNGC 2 cut(s) 235, 479
Bme1390I CCNGG 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 4 cut(s) 166, 394, 481, 671
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 320
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 495
BsaXI ACNNNNNCTCC 6 cut(s) 222, 252, 489, 519, 597, 627
Bse1I ACTGG 2 cut(s) 190, 478
Bse21I CCTNAGG 1 cut(s) 674
Bse3DI GCAATG 3 cut(s) 12, 186, 348
BseGI GGATG 1 cut(s) 556
BseMI GCAATG 3 cut(s) 12, 186, 348
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 190, 478
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 381
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 495
BsmAI GTCTC 4 cut(s) 109, 362, 574, 602
BsmBI CGTCTC 1 cut(s) 109
BsnI GGCC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 5 cut(s) 135, 453, 513, 522, 706
BspACI CCGC 6 cut(s) 110, 366, 379, 385, 396, 477
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 4 cut(s) 166, 394, 481, 671
BspMAI CTGCAG 2 cut(s) 238, 701
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 2 cut(s) 448, 508
BspT107I GGYRCC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 385
BsrDI GCAATG 3 cut(s) 12, 186, 348
BsrI ACTGG 2 cut(s) 190, 478
BssMI GATC 5 cut(s) 135, 453, 513, 522, 706
Bst4CI ACNGT 1 cut(s) 613
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 3 cut(s) 210, 362, 674
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 5 cut(s) 138, 456, 516, 525, 709
BstMAI GTCTC 4 cut(s) 109, 362, 574, 602
BstMBI GATC 5 cut(s) 135, 453, 513, 522, 706
BstMWI GCNNNNNNNGC 4 cut(s) 107, 242, 676, 696
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 234, 697
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 3 cut(s) 453, 513, 706
BstYI RGATCY 3 cut(s) 453, 513, 706
Bsu36I CCTNAGG 1 cut(s) 674
BsuRI GGCC 1 cut(s) 393
BtsCI GGATG 1 cut(s) 556
BtsI GCAGTG 2 cut(s) 417, 628
BtsIMutI CAGTG 3 cut(s) 417, 471, 628
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 1 cut(s) 383
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 392
CviAII CATG 5 cut(s) 169, 400, 640, 680, 687
CviJI RGCY 9 cut(s) 191, 233, 257, 393, 444, 480, 601, 670, 684
CviKI_1 RGCY 9 cut(s) 191, 233, 257, 393, 444, 480, 601, 670, 684
DdeI CTNAG 3 cut(s) 210, 362, 674
DpnI GATC 5 cut(s) 137, 455, 515, 524, 708
DpnII GATC 5 cut(s) 135, 453, 513, 522, 706
Eco57I CTGAAG 1 cut(s) 572
Eco81I CCTNAGG 1 cut(s) 674
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 5 cut(s) 172, 403, 643, 683, 690
FaiI YATR 6 cut(s) 170, 401, 641, 659, 681, 688
FatI CATG 5 cut(s) 168, 399, 639, 679, 686
FauI CCCGC 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 234, 478
FokI GGATG 1 cut(s) 563
Fsp4HI GCNGC 2 cut(s) 234, 478
FspBI CTAG 1 cut(s) 354
FspI TGCGCA 1 cut(s) 21
GlaI GCGC 1 cut(s) 21
GluI GCNGC 2 cut(s) 234, 478
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 1 cut(s) 393
HapII CCGG 1 cut(s) 495
HhaI GCGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 172, 403, 643, 683, 690
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 4 cut(s) 281, 311, 437, 500
HpaII CCGG 1 cut(s) 495
HphI GGTGA 1 cut(s) 133
Hpy188I TCNGA 2 cut(s) 307, 423
Hpy188III TCNNGA 3 cut(s) 252, 315, 590
HpyAV CCTTC 4 cut(s) 51, 154, 596, 696
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 2 cut(s) 123, 546
HpyCH4V TGCA 6 cut(s) 93, 155, 236, 410, 415, 699
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 242, 676, 696
HpyF3I CTNAG 3 cut(s) 210, 362, 674
HpySE526I ACGT 2 cut(s) 123, 546
Hsp92II CATG 5 cut(s) 172, 403, 643, 683, 690
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 135, 453, 513, 522, 706
LmnI GCTCC 7 cut(s) 176, 188, 230, 242, 254, 485, 675
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 541
MaeI CTAG 1 cut(s) 354
MaeII ACGT 2 cut(s) 123, 546
MalI GATC 5 cut(s) 137, 455, 515, 524, 708
MbiI CCGCTC 1 cut(s) 385
MboI GATC 5 cut(s) 135, 453, 513, 522, 706
MboII GAAGA 5 cut(s) 130, 139, 276, 320, 457
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 3 cut(s) 453, 513, 706
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 4 cut(s) 64, 267, 318, 528
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 2 cut(s) 221, 702
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 495
MspR9I CCNGG 1 cut(s) 495
MunI CAATTG 1 cut(s) 267
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 4 cut(s) 107, 242, 676, 696
NciI CCSGG 1 cut(s) 495
NdeII GATC 5 cut(s) 135, 453, 513, 522, 706
NlaIII CATG 5 cut(s) 172, 403, 643, 683, 690
NlaIV GGNNCC 4 cut(s) 166, 394, 481, 671
NsbI TGCGCA 1 cut(s) 21
NspI RCATGY 1 cut(s) 643
PciI ACATGT 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 4 cut(s) 281, 311, 437, 500
PkrI GCNGC 2 cut(s) 235, 479
PscI ACATGT 1 cut(s) 639
Psp1406I AACGTT 1 cut(s) 123
PspN4I GGNNCC 4 cut(s) 166, 394, 481, 671
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 2 cut(s) 238, 701
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 453, 513, 706
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 2 cut(s) 234, 478
Sau3AI GATC 5 cut(s) 135, 453, 513, 522, 706
Sau96I GGNCC 1 cut(s) 392
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 167
SetI ASST 9 cut(s) 126, 146, 193, 235, 259, 446, 536, 549, 603
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 2 cut(s) 234, 697
Sse9I AATT 4 cut(s) 64, 267, 318, 528
SsiI CCGC 6 cut(s) 110, 366, 379, 385, 396, 477
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 2 cut(s) 126, 549
TaqI TCGA 2 cut(s) 72, 468
TasI AATT 4 cut(s) 64, 267, 318, 528
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 4 cut(s) 281, 311, 437, 500
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 3 cut(s) 417, 478, 635
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 58, 324
TspRI CASTG 3 cut(s) 417, 478, 635
XapI RAATTY 1 cut(s) 64
XceI RCATGY 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.