RLG00000016796

NEP1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006207
Physical Location & Seq
Forward (+)
10068373 .. 10073014
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016796

Sequence Viewer

Length: 1014 bp
ATGTCGTGGTTCAACCTCTTAATCATTAGGGCCGGCATTTACTTGATGTCAGCTCCATTAGTATTGGAGATTCGTCAGATCACAGTGCTTAGGCGTCCACCTCCACTTGACGAGTTCGCGGCCGTTGGATTTGTTGTTGGAAATGCAATTCCAGCTCTTGAAGATCTCCTTTCAGGTGTTGTCGTACTTAGGAGAAGCATTAGGGTTGTAGATCCAGGCTCCATCGGAGTAGTCGCAGGACCCGACCAGCTCCGTCAAGTAGGATCCCGGGGGTTTCTGGAATTCGAGGGAGTTGTGAGGTTGGGAAGGAACGGAGGAGCGCACCCATCTCGTTTCTCGTCCTCCTTTTCCTATTCCTTTGGAAATGTCTTCGAGAGGGTTAAAGATTTTTGCAACTTTGCGCTTTCTGCGATTATTGGGAACGTTTTCTCTGCGATCTTCACCTTCTTCTTTGCTTTGGTGGGCACCTTGTTGGGAGCTATGACTGGAGCTTTGATAGGACAAGAAACCGAGAGCGGATTTGTTAGGGGTGCTGCTGTTGGAGCAATCTCAGGAGCTGTTTTCTCCATTGAAGTGTTTGAATCATCCCTTATTCTATGGCAATCAGATGAATCCGGAATTGGGTGTCTTCTCTACTTGATTGATGTCATTGCGAGTCTCCTAAGTGGTAGATTAGTACGTGAGCGAATTGGTCCAGCCATGTTGAGTGCAGTGCAAAGTCAGATGGTTGCTGTTGAATCAAGCTTTGAAGAGATTCCTAACATCTTTGACACTGGTGGGGCCAAAGGATTGCCTGGAGATTCAGTTGAAAAGATCCCAAAGATCATCATCACTAGCAACAATAATGTGGATGCTTCTGGAGATAAAGTCTCATGTTCAGTGTGCCTTCAGGACTTTCAGCTTGGAGAGACAGTCAGAAGTTTACCACAATGCCATCACATGTTTCACCTGCCATGTATAGATAAGTGGCTCCTGAGGCATGGCTCTTGCCCACTTTGCAGAAGGGATCTGTGA

Protein Analysis

338

Amino Acids

36.3

Weight (kDa)

5.78

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-zipper_YMGG PF13441 152 - 187 4.9e-07 YMGG-like Gly-zipper
zf-RING_2 PF13639 291 - 334 4.9e-13 Ring finger domain
zf-RING_11 PF17123 292 - 320 1.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 292 - 333 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 307 - 334 9.4e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 957
Acc36I ACCTGC 1 cut(s) 957
AccB1I GGYRCC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 516
AccII CGCG 1 cut(s) 119
AccIII TCCGGA 1 cut(s) 614
AciI CCGC 2 cut(s) 119, 516
AclI AACGTT 1 cut(s) 423
AclWI GGATC 4 cut(s) 206, 258, 271, 808
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 94
AfaI GTAC 2 cut(s) 186, 678
AfiI CCNNNNNNNGG 1 cut(s) 621
AflIII ACRYGT 1 cut(s) 939
AgsI TTSAA 7 cut(s) 13, 161, 572, 581, 737, 749, 809
AjnI CCWGG 2 cut(s) 214, 793
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AluBI AGCT 8 cut(s) 53, 155, 250, 479, 491, 557, 744, 901
AluI AGCT 8 cut(s) 53, 155, 250, 479, 491, 557, 744, 901
Alw26I GTCTC 3 cut(s) 662, 874, 902
AlwI GGATC 4 cut(s) 206, 258, 271, 808
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 267
Aor13HI TCCGGA 1 cut(s) 614
AoxI GGCC 3 cut(s) 30, 120, 780
ApeKI GCWGC 1 cut(s) 533
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
Asp700I GAANNNNTTC 2 cut(s) 425, 753
AspLEI GCGC 2 cut(s) 322, 403
AspS9I GGNCC 4 cut(s) 30, 239, 692, 780
AsuC2I CCSGG 2 cut(s) 268, 269
AsuHPI GGTGA 2 cut(s) 433, 938
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 2 cut(s) 239, 692
AxyI CCTNAGG 1 cut(s) 974
BaeGI GKGCMC 1 cut(s) 467
BamHI GGATCC 1 cut(s) 263
BanI GGYRCC 1 cut(s) 464
BbsI GAAGAC 2 cut(s) 361, 620
BbvI GCAGC 1 cut(s) 520
BccI CCATC 4 cut(s) 230, 334, 718, 942
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 2 cut(s) 216, 795
BcnI CCSGG 2 cut(s) 268, 269
BcoDI GTCTC 3 cut(s) 662, 874, 902
BfaI CTAG 1 cut(s) 834
BfuAI ACCTGC 1 cut(s) 957
BglII AGATCT 1 cut(s) 163
BisI GCNGC 2 cut(s) 120, 534
BlsI GCNGC 2 cut(s) 121, 535
Bme1390I CCNGG 4 cut(s) 216, 268, 269, 795
Bme18I GGWCC 2 cut(s) 239, 692
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 4 cut(s) 30, 239, 692, 780
BmiI GGNNCC 6 cut(s) 220, 241, 265, 466, 781, 971
BmrFI CCNGG 4 cut(s) 216, 268, 269, 795
BmsI GCATC 1 cut(s) 841
BpiI GAAGAC 2 cut(s) 361, 620
BpmI CTGGAG 3 cut(s) 507, 816, 879
Bpu10I CCTNAGC 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 268, 269
BsaAI YACGTR 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 827
BsaHI GRCGYC 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 267, 268
BsaWI WCCGGW 1 cut(s) 614
BsaXI ACNNNNNCTCC 6 cut(s) 789, 819, 852, 882, 897, 927
Bsc4I CCNNNNNNNGG 1 cut(s) 621
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 2 cut(s) 490, 778
Bse21I CCTNAGG 1 cut(s) 974
Bse3DI GCAATG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 827
BseAI TCCGGA 1 cut(s) 614
BseBI CCWGG 2 cut(s) 216, 795
BseDI CCNNGG 2 cut(s) 267, 268
BseGI GGATG 2 cut(s) 584, 856
BseJI GATNNNNATC 1 cut(s) 827
BseLI CCNNNNNNNGG 1 cut(s) 621
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 2 cut(s) 564, 965
BseNI ACTGG 2 cut(s) 490, 778
BseRI GAGGAG 1 cut(s) 330
BseSI GKGCMC 1 cut(s) 467
BseX3I CGGCCG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 520
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 3 cut(s) 32, 122, 782
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 3 cut(s) 33, 268, 615
BslI CCNNNNNNNGG 1 cut(s) 621
BsmAI GTCTC 3 cut(s) 662, 874, 902
BsnI GGCC 3 cut(s) 32, 122, 782
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 467
Bsp13I TCCGGA 1 cut(s) 614
Bsp143I GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
BspACI CCGC 2 cut(s) 119, 516
BspANI GGCC 3 cut(s) 32, 122, 782
BspCNI CTCAG 2 cut(s) 563, 966
BspEI TCCGGA 1 cut(s) 614
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 6 cut(s) 220, 241, 265, 466, 781, 971
BspMI ACCTGC 1 cut(s) 957
BspPI GGATC 4 cut(s) 206, 258, 271, 808
BspT107I GGYRCC 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 2 cut(s) 490, 778
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 2 cut(s) 267, 268
BssMI GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
BssNI GRCGYC 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 216, 795
Bst4CI ACNGT 2 cut(s) 85, 913
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 94
BstBAI YACGTR 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 5 cut(s) 89, 188, 550, 662, 974
BstF5I GGATG 2 cut(s) 584, 856
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 2 cut(s) 322, 403
BstKTI GATC 8 cut(s) 81, 166, 214, 266, 438, 816, 825, 1009
BstMAI GTCTC 3 cut(s) 662, 874, 902
BstMBI GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 5 cut(s) 152, 407, 542, 976, 996
BstNI CCWGG 2 cut(s) 216, 795
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 4 cut(s) 214, 266, 267, 793
BstSLI GKGCMC 1 cut(s) 467
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 361, 620
BstX2I RGATCY 5 cut(s) 163, 211, 263, 813, 1006
BstYI RGATCY 5 cut(s) 163, 211, 263, 813, 1006
BstZI CGGCCG 1 cut(s) 120
Bsu36I CCTNAGG 1 cut(s) 974
BsuRI GGCC 3 cut(s) 32, 122, 782
BtsCI GGATG 2 cut(s) 584, 856
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 4 cut(s) 90, 717, 771, 885
BveI ACCTGC 1 cut(s) 957
Cac8I GCNNGC 1 cut(s) 34
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 2 cut(s) 322, 403
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 4 cut(s) 30, 239, 692, 780
Cfr9I CCCGGG 1 cut(s) 267
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 185, 677
CviAII CATG 5 cut(s) 700, 873, 940, 954, 980
CviQI GTAC 2 cut(s) 185, 677
DdeI CTNAG 5 cut(s) 89, 188, 550, 662, 974
DpnI GATC 8 cut(s) 80, 165, 213, 265, 437, 815, 824, 1008
DpnII GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
EaeI YGGCCR 1 cut(s) 120
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EclXI CGGCCG 1 cut(s) 120
Eco47I GGWCC 2 cut(s) 239, 692
Eco52I CGGCCG 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 872
Eco81I CCTNAGG 1 cut(s) 974
Eco88I CYCGRG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 239
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 214, 793
FaeI CATG 5 cut(s) 703, 876, 943, 957, 983
FaiI YATR 8 cut(s) 482, 598, 701, 874, 941, 955, 959, 981
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FatI CATG 5 cut(s) 699, 872, 939, 953, 979
Fnu4HI GCNGC 2 cut(s) 120, 534
FokI GGATG 2 cut(s) 571, 863
Fsp4HI GCNGC 2 cut(s) 120, 534
FspBI CTAG 1 cut(s) 834
GlaI GCGC 2 cut(s) 321, 402
GluI GCNGC 2 cut(s) 120, 534
GsuI CTGGAG 3 cut(s) 507, 816, 879
HaeIII GGCC 3 cut(s) 32, 122, 782
HapII CCGG 3 cut(s) 33, 268, 615
HgaI GACGC 1 cut(s) 83
HhaI GCGC 2 cut(s) 322, 403
Hin1I GRCGYC 1 cut(s) 94
Hin1II CATG 5 cut(s) 703, 876, 943, 957, 983
Hin6I GCGC 2 cut(s) 320, 401
HinP1I GCGC 2 cut(s) 320, 401
HindIII AAGCTT 1 cut(s) 742
HinfI GANTC 7 cut(s) 70, 581, 611, 655, 737, 754, 800
HpaII CCGG 3 cut(s) 33, 268, 615
HphI GGTGA 2 cut(s) 433, 938
Hpy166II GTNNAC 2 cut(s) 98, 923
Hpy188I TCNGA 5 cut(s) 78, 227, 607, 723, 917
Hpy188III TCNNGA 8 cut(s) 158, 278, 373, 552, 615, 858, 890, 973
Hpy8I GTNNAC 2 cut(s) 98, 923
HpyAV CCTTC 4 cut(s) 300, 454, 896, 996
HpyCH4III ACNGT 2 cut(s) 85, 913
HpyCH4IV ACGT 2 cut(s) 423, 679
HpyCH4V TGCA 5 cut(s) 146, 393, 710, 715, 999
HpyF10VI GCNNNNNNNGC 5 cut(s) 152, 407, 542, 976, 996
HpyF3I CTNAG 5 cut(s) 89, 188, 550, 662, 974
HpySE526I ACGT 2 cut(s) 423, 679
Hsp92I GRCGYC 1 cut(s) 94
Hsp92II CATG 5 cut(s) 703, 876, 943, 957, 983
HspAI GCGC 2 cut(s) 320, 401
Kpn2I TCCGGA 1 cut(s) 614
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
LmnI GCTCC 9 cut(s) 58, 224, 255, 317, 476, 488, 542, 554, 975
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 1 cut(s) 841
MaeI CTAG 1 cut(s) 834
MaeII ACGT 2 cut(s) 423, 679
MalI GATC 8 cut(s) 80, 165, 213, 265, 437, 815, 824, 1008
MbiI CCGCTC 1 cut(s) 516
MboI GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
MboII GAAGA 6 cut(s) 173, 361, 430, 439, 620, 761
MflI RGATCY 5 cut(s) 163, 211, 263, 813, 1006
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 4 cut(s) 147, 281, 618, 687
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 3 cut(s) 106, 118, 520
MnlI CCTC 8 cut(s) 26, 111, 280, 291, 308, 352, 369, 969
MroI TCCGGA 1 cut(s) 614
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 2 cut(s) 425, 753
MseI TTAA 2 cut(s) 20, 381
MslI CAYNNNNRTG 1 cut(s) 572
MspI CCGG 3 cut(s) 33, 268, 615
MspR9I CCNGG 4 cut(s) 216, 268, 269, 795
MvaI CCWGG 2 cut(s) 216, 795
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 5 cut(s) 152, 407, 542, 976, 996
NaeI GCCGGC 1 cut(s) 34
NciI CCSGG 2 cut(s) 268, 269
NdeII GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 5 cut(s) 703, 876, 943, 957, 983
NlaIV GGNNCC 6 cut(s) 220, 241, 265, 466, 781, 971
NspI RCATGY 1 cut(s) 943
PaqCI CACCTGC 1 cut(s) 957
PciI ACATGT 1 cut(s) 939
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 2 cut(s) 425, 753
PfeI GAWTC 6 cut(s) 70, 581, 611, 737, 754, 800
PkrI GCNGC 2 cut(s) 121, 535
PleI GAGTC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 663
Ppu21I YACGTR 1 cut(s) 680
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 939
Psp1406I AACGTT 1 cut(s) 423
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 214, 793
PspGI CCWGG 2 cut(s) 214, 793
PspN4I GGNNCC 6 cut(s) 220, 241, 265, 466, 781, 971
PspPI GGNCC 4 cut(s) 30, 239, 692, 780
PspPPI RGGWCCY 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 5 cut(s) 163, 211, 263, 813, 1006
RsaI GTAC 2 cut(s) 186, 678
RsaNI GTAC 2 cut(s) 185, 677
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 2 cut(s) 20, 381
SatI GCNGC 2 cut(s) 120, 534
Sau3AI GATC 8 cut(s) 78, 163, 211, 263, 435, 813, 822, 1006
Sau96I GGNCC 4 cut(s) 30, 239, 692, 780
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 4 cut(s) 216, 268, 269, 795
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 1 cut(s) 841
SinI GGWCC 2 cut(s) 239, 692
SmaI CCCGGG 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 572
Sse9I AATT 4 cut(s) 147, 281, 618, 687
SsiI CCGC 2 cut(s) 119, 516
SspMI CTAG 1 cut(s) 834
StyD4I CCNGG 4 cut(s) 214, 266, 267, 793
TaaI ACNGT 2 cut(s) 85, 913
TaiI ACGT 2 cut(s) 426, 682
TaqI TCGA 2 cut(s) 285, 372
TasI AATT 4 cut(s) 147, 281, 618, 687
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 6 cut(s) 70, 581, 611, 737, 754, 800
Tru1I TTAA 2 cut(s) 20, 381
Tru9I TTAA 2 cut(s) 20, 381
TscAI CASTG 4 cut(s) 90, 717, 778, 885
TseI GCWGC 1 cut(s) 533
TspDTI ATGAA 1 cut(s) 624
TspGWI ACGGA 2 cut(s) 242, 327
TspMI CCCGGG 1 cut(s) 267
TspRI CASTG 4 cut(s) 90, 717, 778, 885
VpaK11BI GGWCC 2 cut(s) 239, 692
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 943
XmaI CCCGGG 1 cut(s) 267
XmnI GAANNNNTTC 2 cut(s) 425, 753
XspI CTAG 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.