MD02G1134500.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005584
Physical Location & Seq
Reverse (-)
10898803 .. 10903379
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1134500.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGATGGAGTCAATCGAGTCTACTGTCCCACCCGGTTTCCGCTTTCATCCGACCGATGAAGAGCTCGTCGGGTACTATCTTAGAAAGAAAGTTGCCTCCCAGAAGATCGATCTTGATGTCATCAGAGACATTGATCTCTACAGAATCGAACCATGGGATCTTCAAGAGAGATGTCGAATCGGATATGAAGAGCAGAACGAGTGGTACTTCTTCAGCCACAAAGACAAGAAGTATCCAACTGGGACAAGGACCAACAGAGCAACCATGGCTGGGTTTTGGAAAGCAACGGGTCGAGACAAATCAGTATACGATAAGGCAAAACTGATCGGGATGAGGAAAACCCTTGTCTTCTACAAAGGAAGGGCTCCAAATGGACAGAAGAGCGATTGGATCATGCACGAATACAGACTCGAATCCGATGAAAATGGACCTCCGCAGGAAGAAGGATGGGTCGTATGCAGAGCATTCAAGAAGAGAACCAGTAGTCAAAACAAAAGCATCGAAGGGTGGGACTCAAGCTACTTCTATGAAGAACTCAACGGTCTCACTTCCGTCGCTGATCCAACCAATTATATGATCTCGAGGCAGCCCCAGAGCTTTTTAAGCCAGAATTTCATGTGCAAGCAAGAGACAGAAGCTGACAGTTTGAACTTCCTGCACTCTGATCCGTTTGTCCAGCTTCCTCAGCTTGAGAGCCCGTCTCTTCCCTTAATAAAGCGGCCGAGCTCAACGTCTCTTATATCGGAGAATAACATCGAAATAGAAGACCAGCAAAGTATTCGAGGATGCAACGATGCCAAGAAGGTTACTGACTGGAGGGCTCTGGACAAGTTTGTGGCTTCACAGTTGAGTCAAGAGGAGAGGTATGAAGGTGATGGAGGGGCAAGCTTTGGTGCAAATGATGAATCAGATATGGCATTGTTGTTGTTGCAGAGTAGTAGTGCTAGGGATGAAGATGATCAACTGGGAAACAAGTTAAATGGGTTCCTAAATTCGACCACTGACTGTGATATTGGGATATGCATATTTGAAAACTAA

Protein Analysis

346

Amino Acids

39.62

Weight (kDa)

4.93

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 137 2.3e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 20, 306
AciI CCGC 3 cut(s) 40, 434, 718
AclWI GGATC 4 cut(s) 165, 398, 554, 659
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 2 cut(s) 610, 991
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 2 cut(s) 74, 206
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 4 cut(s) 164, 469, 649, 1031
AluBI AGCT 8 cut(s) 64, 519, 597, 638, 679, 688, 726, 888
AluI AGCT 8 cut(s) 64, 519, 597, 638, 679, 688, 726, 888
Alw21I GWGCWC 2 cut(s) 66, 728
Alw26I GTCTC 6 cut(s) 120, 288, 548, 623, 705, 738
AlwI GGATC 4 cut(s) 165, 398, 554, 659
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 1 cut(s) 719
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 2 cut(s) 610, 991
AspS9I GGNCC 2 cut(s) 249, 428
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 2 cut(s) 249, 428
BanII GRGCYC 5 cut(s) 66, 367, 698, 728, 823
BbsI GAAGAC 2 cut(s) 340, 771
Bbv12I GWGCWC 2 cut(s) 66, 728
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 1 cut(s) 598
BccI CCATC 2 cut(s) 441, 869
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 1 cut(s) 958
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 6 cut(s) 120, 288, 548, 623, 705, 738
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 1 cut(s) 139
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 2 cut(s) 587, 719
BlsI GCNGC 2 cut(s) 588, 720
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 249, 428
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 249, 428
BmiI GGNNCC 2 cut(s) 366, 986
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 2 cut(s) 249, 974
BmsI GCATC 3 cut(s) 507, 776, 784
BmuI ACTGGG 2 cut(s) 249, 974
BpiI GAAGAC 2 cut(s) 340, 771
BplI GAGNNNNNCTC 2 cut(s) 685, 717
BpmI CTGGAG 1 cut(s) 835
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 2 cut(s) 499, 710
BpuMI CCSGG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 152, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 4 cut(s) 244, 480, 818, 969
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 152, 264
BseGI GGATG 5 cut(s) 46, 336, 452, 791, 955
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 4 cut(s) 244, 480, 818, 969
BseRI GAGGAG 1 cut(s) 872
BseX3I CGGCCG 1 cut(s) 719
BseXI GCAGC 1 cut(s) 598
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 641
Bsh1285I CGRYCG 2 cut(s) 54, 722
BshFI GGCC 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 2 cut(s) 54, 722
BsiHKAI GWGCWC 2 cut(s) 66, 728
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 3 cut(s) 11, 256, 524
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 6 cut(s) 120, 288, 548, 623, 705, 738
BsmBI CGTCTC 2 cut(s) 705, 738
BsmFI GGGAC 3 cut(s) 11, 256, 524
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 721
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 1 cut(s) 580
Bsp1286I GDGCHC 5 cut(s) 66, 367, 698, 728, 823
Bsp19I CCATGG 2 cut(s) 152, 264
BspACI CCGC 3 cut(s) 40, 434, 718
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 1 cut(s) 697
BspDI ATCGAT 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 366, 986
BspPI GGATC 4 cut(s) 165, 398, 554, 659
BspQI GCTCTTC 3 cut(s) 54, 183, 374
BspTNI GGTCTC 1 cut(s) 548
BsrI ACTGG 4 cut(s) 244, 480, 818, 969
BssECI CCNNGG 2 cut(s) 152, 264
BssNAI GTATAC 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 152, 264
Bst1107I GTATAC 1 cut(s) 307
Bst4CI ACNGT 5 cut(s) 25, 542, 644, 846, 1007
Bst6I CTCTTC 5 cut(s) 54, 183, 374, 467, 708
BstC8I GCNNGC 2 cut(s) 623, 886
BstDEI CTNAG 2 cut(s) 80, 684
BstDSI CCRYGG 2 cut(s) 152, 264
BstF5I GGATG 5 cut(s) 46, 336, 452, 791, 955
BstMAI GTCTC 6 cut(s) 120, 288, 548, 623, 705, 738
BstMCI CGRYCG 2 cut(s) 54, 722
BstMWI GCNNNNNNNGC 3 cut(s) 266, 603, 685
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 598
BstV2I GAAGAC 2 cut(s) 340, 771
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 307
BstZI CGGCCG 1 cut(s) 719
Bsu15I ATCGAT 1 cut(s) 108
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 1 cut(s) 721
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 2 cut(s) 152, 264
BtsCI GGATG 5 cut(s) 46, 336, 452, 791, 955
BtsIMutI CAGTG 1 cut(s) 999
Cac8I GCNNGC 2 cut(s) 623, 886
CaiI CAGNNNCTG 1 cut(s) 638
Cfr13I GGNCC 2 cut(s) 249, 428
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 2 cut(s) 73, 205
CviAII CATG 4 cut(s) 153, 265, 394, 616
CviQI GTAC 2 cut(s) 73, 205
DdeI CTNAG 2 cut(s) 80, 684
EaeI YGGCCR 1 cut(s) 719
EagI CGGCCG 1 cut(s) 719
Eam1104I CTCTTC 5 cut(s) 54, 183, 374, 467, 708
EarI CTCTTC 5 cut(s) 54, 183, 374, 467, 708
Ecl136II GAGCTC 2 cut(s) 64, 726
EclXI CGGCCG 1 cut(s) 719
Eco130I CCWWGG 2 cut(s) 152, 264
Eco24I GRGCYC 5 cut(s) 66, 367, 698, 728, 823
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 249, 428
Eco52I CGGCCG 1 cut(s) 719
Eco53kI GAGCTC 2 cut(s) 64, 726
Eco57I CTGAAG 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 2 cut(s) 64, 726
EcoT14I CCWWGG 2 cut(s) 152, 264
EcoT22I ATGCAT 1 cut(s) 1025
EcoT38I GRGCYC 5 cut(s) 66, 367, 698, 728, 823
ErhI CCWWGG 2 cut(s) 152, 264
Esp3I CGTCTC 2 cut(s) 705, 738
FaeI CATG 4 cut(s) 156, 268, 397, 619
FaqI GGGAC 3 cut(s) 11, 256, 524
FatI CATG 4 cut(s) 152, 264, 393, 615
FbaI TGATCA 1 cut(s) 958
FblI GTMKAC 2 cut(s) 20, 306
Fnu4HI GCNGC 2 cut(s) 587, 719
FokI GGATG 5 cut(s) 33, 343, 459, 798, 962
FriOI GRGCYC 5 cut(s) 66, 367, 698, 728, 823
Fsp4HI GCNGC 2 cut(s) 587, 719
FspBI CTAG 1 cut(s) 945
GluI GCNGC 2 cut(s) 587, 719
GsaI CCCAGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 835
HaeIII GGCC 1 cut(s) 721
HapII CCGG 1 cut(s) 33
Hin1II CATG 4 cut(s) 156, 268, 397, 619
HindIII AAGCTT 1 cut(s) 886
HinfI GANTC 9 cut(s) 8, 17, 144, 177, 408, 413, 512, 850, 905
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 884
Hpy166II GTNNAC 2 cut(s) 21, 307
Hpy188I TCNGA 7 cut(s) 51, 125, 182, 418, 664, 745, 910
Hpy188III TCNNGA 8 cut(s) 113, 164, 293, 328, 469, 580, 824, 854
Hpy8I GTNNAC 2 cut(s) 21, 307
Hpy99I CGWCG 2 cut(s) 71, 557
HpyAV CCTTC 5 cut(s) 354, 437, 497, 796, 863
HpyCH4III ACNGT 5 cut(s) 25, 542, 644, 846, 1007
HpyCH4IV ACGT 1 cut(s) 731
HpyCH4V TGCA 8 cut(s) 397, 459, 621, 658, 789, 896, 931, 1023
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 603, 685
HpyF3I CTNAG 2 cut(s) 80, 684
HpySE526I ACGT 1 cut(s) 731
Hsp92II CATG 4 cut(s) 156, 268, 397, 619
Ksp22I TGATCA 1 cut(s) 958
LguI GCTCTTC 3 cut(s) 54, 183, 374
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 598
LweI GCATC 3 cut(s) 507, 776, 784
MaeI CTAG 1 cut(s) 945
MaeII ACGT 1 cut(s) 731
MaeIII GTNAC 1 cut(s) 805
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 5 cut(s) 66, 367, 698, 728, 823
MluCI AATT 3 cut(s) 568, 610, 991
MlyI GAGTC 5 cut(s) 17, 26, 402, 506, 859
MmeI TCCRAC 3 cut(s) 74, 260, 587
Mph1103I ATGCAT 1 cut(s) 1025
MseI TTAA 3 cut(s) 602, 710, 977
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 464
MwoI GCNNNNNNNGC 3 cut(s) 266, 603, 685
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 2 cut(s) 152, 264
NlaIII CATG 4 cut(s) 156, 268, 397, 619
NlaIV GGNNCC 2 cut(s) 366, 986
NmeAIII GCCGAG 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 1025
PaeR7I CTCGAG 1 cut(s) 580
PciSI GCTCTTC 3 cut(s) 54, 183, 374
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 4 cut(s) 144, 177, 413, 905
PkrI GCNGC 2 cut(s) 588, 720
PleI GAGTC 5 cut(s) 16, 25, 402, 506, 858
PpsI GAGTC 5 cut(s) 16, 25, 402, 506, 858
Psp124BI GAGCTC 2 cut(s) 66, 728
PspFI CCCAGC 1 cut(s) 269
PspN4I GGNNCC 2 cut(s) 366, 986
PspPI GGNCC 2 cut(s) 249, 428
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 2 cut(s) 74, 206
RsaNI GTAC 2 cut(s) 73, 205
SacI GAGCTC 2 cut(s) 66, 728
SapI GCTCTTC 3 cut(s) 54, 183, 374
SaqAI TTAA 3 cut(s) 602, 710, 977
SatI GCNGC 2 cut(s) 587, 719
Sau96I GGNCC 2 cut(s) 249, 428
SchI GAGTC 5 cut(s) 17, 26, 402, 506, 859
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 5 cut(s) 66, 367, 698, 728, 823
SfaNI GCATC 3 cut(s) 507, 776, 784
SfcI CTRYAG 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 580
SinI GGWCC 2 cut(s) 249, 428
SlaI CTCGAG 1 cut(s) 580
SmlI CTYRAG 3 cut(s) 514, 580, 689
SmoI CTYRAG 3 cut(s) 514, 580, 689
Sse9I AATT 3 cut(s) 568, 610, 991
SsiI CCGC 3 cut(s) 40, 434, 718
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 2 cut(s) 66, 728
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 2 cut(s) 152, 264
TaaI ACNGT 5 cut(s) 25, 542, 644, 846, 1007
TaiI ACGT 1 cut(s) 734
TaqII GACCGA 1 cut(s) 68
TasI AATT 3 cut(s) 568, 610, 991
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 4 cut(s) 144, 177, 413, 905
Tru1I TTAA 3 cut(s) 602, 710, 977
Tru9I TTAA 3 cut(s) 602, 710, 977
TscAI CASTG 1 cut(s) 1006
TseI GCWGC 1 cut(s) 586
TspDTI ATGAA 9 cut(s) 35, 72, 201, 435, 543, 604, 882, 918, 966
TspGWI ACGGA 2 cut(s) 541, 657
TspRI CASTG 1 cut(s) 1006
VpaK11BI GGWCC 2 cut(s) 249, 428
XapI RAATTY 2 cut(s) 610, 991
XhoI CTCGAG 1 cut(s) 580
XmiI GTMKAC 2 cut(s) 20, 306
XspI CTAG 1 cut(s) 945
Zsp2I ATGCAT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.