MD15G1247800.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005584
Physical Location & Seq
Reverse (-)
20579332 .. 20584307
4976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1247800.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGATGGAGTCAATCGAGTGTACTGTCCCACCCGGTTTCCGCTTTCATCCGACGGATGAAGAGCTCGTCGGGTACTATCTTAGAAAGAAAGTTGCCTCCCAGAAGATCGATCTTGATGTCATCAGAGACATTGATCTCTACAGAATCGAACCATGGGATCTTCAAGAGAGATGTCGGATCGGGTATGAAGAGCAGAACGAGTGGTACTTCTTTAGCCACAAAGACAAGAAGTATCCAACTGGGACAAGGACCAACAGAGCAACCATGGCTGGGTTCTGGAAGGCAACGGGTCGAGACAAATCAGTATACGATAAGGCAAAACTGATCGGGATGAGGAAAACTCTTGTCTTCTACAAAGGAAGGGCTCCAAATGGACAGAAGACTGATTGGATCATGCACGAATACAGACTCGAATCTGACGAAAATGGACCTCCGCAGGAAGAAGGATGGGTGGTATGCAGAGCATTCAAGAAGAGAATCAGTAGCCAAAACAAAAGCATTGAAGGCTGGGACTCAAGCTACTTCTACGAAGAACTTAACGGTCTCACTTCCGTCGCTGATCCAACGGATTTTATGATCTCGAGGCAGCCCCAGAGCTTTTTAAGCCAGAATTTCATGTGCAAGCAAGAGACAGAAGCTGATAGTTTGAACTTCCTGCACTCTGATCCGTTTGTCCAGCTTCCTCAGCTTGAGAGCCCGTCTCTTCCCTTAATAAAGCGGCCGACCTCAATGTCCCTTATCTCTGAGAACAATACTGAGTTAGAAGACGAGCAAAGTATTCGAGGGTGTAACAATGCGGAGAAGGTTACTGACTGGAGGGCTCTGGACAAGTTTGTGGCTTCACAGCTGAGTCAAGAGGAGAGGTATGAAGGTGATGGAGAGGCAAGCTTTGGTGCACATGATGAATCAGATATGTCATTGTTGTTGTTGCAGAGTAGTAGTGCTAGAGATGATGATGATCAAGTGGAGAACAAGTTAAATGGGTTCCTGAATTCGAGTCCTGACTGTGATATTGGGATATGCATATTTGAAAAATAA

Protein Analysis

346

Amino Acids

39.83

Weight (kDa)

4.83

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 137 7.6e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 306
AciI CCGC 4 cut(s) 40, 434, 718, 797
AclWI GGATC 5 cut(s) 165, 185, 398, 554, 659
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 2 cut(s) 610, 991
AfaI GTAC 3 cut(s) 22, 74, 206
AfiI CCNNNNNNNGG 1 cut(s) 270
AgsI TTSAA 5 cut(s) 164, 469, 503, 649, 1031
AluBI AGCT 8 cut(s) 64, 519, 597, 638, 679, 688, 847, 888
AluI AGCT 8 cut(s) 64, 519, 597, 638, 679, 688, 847, 888
Alw21I GWGCWC 2 cut(s) 66, 898
Alw26I GTCTC 5 cut(s) 120, 288, 548, 623, 705
Alw44I GTGCAC 1 cut(s) 894
AlwI GGATC 5 cut(s) 165, 185, 398, 554, 659
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 1 cut(s) 719
ApaLI GTGCAC 1 cut(s) 894
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 2 cut(s) 610, 991
AspS9I GGNCC 2 cut(s) 249, 428
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 884
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 2 cut(s) 249, 428
BaeGI GKGCMC 1 cut(s) 898
BanII GRGCYC 4 cut(s) 66, 367, 698, 823
BbsI GAAGAC 3 cut(s) 340, 386, 771
Bbv12I GWGCWC 2 cut(s) 66, 898
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 1 cut(s) 598
BccI CCATC 2 cut(s) 441, 869
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 1 cut(s) 958
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 5 cut(s) 120, 288, 548, 623, 705
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 1 cut(s) 139
BfuI GTATCC 1 cut(s) 243
BisI GCNGC 2 cut(s) 587, 719
BlsI GCNGC 2 cut(s) 588, 720
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 249, 428
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 2 cut(s) 249, 428
BmiI GGNNCC 2 cut(s) 366, 986
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 249
BpiI GAAGAC 3 cut(s) 340, 386, 771
BplI GAGNNNNNCTC 4 cut(s) 325, 357, 685, 717
BpmI CTGGAG 1 cut(s) 835
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 2 cut(s) 499, 710
BpuMI CCSGG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 152, 264
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse1I ACTGG 2 cut(s) 244, 818
Bse8I GATNNNNATC 1 cut(s) 957
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 152, 264
BseGI GGATG 4 cut(s) 46, 61, 336, 452
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMII CTCAG 4 cut(s) 698, 735, 747, 839
BseNI ACTGG 2 cut(s) 244, 818
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 1 cut(s) 898
BseX3I CGGCCG 1 cut(s) 719
BseXI GCAGC 1 cut(s) 598
BseYI CCCAGC 2 cut(s) 269, 507
BsgI GTGCAG 1 cut(s) 641
Bsh1285I CGRYCG 1 cut(s) 722
BshFI GGCC 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 722
BsiHKAI GWGCWC 2 cut(s) 66, 898
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 4 cut(s) 11, 256, 524, 718
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 5 cut(s) 120, 288, 548, 623, 705
BsmBI CGTCTC 1 cut(s) 705
BsmFI GGGAC 4 cut(s) 11, 256, 524, 718
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 721
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 1 cut(s) 580
Bsp1286I GDGCHC 5 cut(s) 66, 367, 698, 823, 898
Bsp19I CCATGG 2 cut(s) 152, 264
BspACI CCGC 4 cut(s) 40, 434, 718, 797
BspANI GGCC 1 cut(s) 721
BspCNI CTCAG 4 cut(s) 697, 736, 748, 840
BspDI ATCGAT 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 366, 986
BspPI GGATC 5 cut(s) 165, 185, 398, 554, 659
BspQI GCTCTTC 2 cut(s) 54, 183
BspTNI GGTCTC 1 cut(s) 548
BsrI ACTGG 2 cut(s) 244, 818
BssECI CCNNGG 2 cut(s) 152, 264
BssNAI GTATAC 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 152, 264
Bst1107I GTATAC 1 cut(s) 307
Bst4CI ACNGT 3 cut(s) 25, 542, 1007
Bst6I CTCTTC 4 cut(s) 54, 183, 467, 708
BstC8I GCNNGC 2 cut(s) 623, 886
BstDEI CTNAG 5 cut(s) 80, 684, 744, 756, 848
BstDSI CCRYGG 2 cut(s) 152, 264
BstF5I GGATG 4 cut(s) 46, 61, 336, 452
BstMAI GTCTC 5 cut(s) 120, 288, 548, 623, 705
BstMCI CGRYCG 1 cut(s) 722
BstMWI GCNNNNNNNGC 4 cut(s) 266, 504, 603, 685
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 898
BstV1I GCAGC 1 cut(s) 598
BstV2I GAAGAC 3 cut(s) 340, 386, 771
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 307
BstZI CGGCCG 1 cut(s) 719
Bsu15I ATCGAT 1 cut(s) 108
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 1 cut(s) 721
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 2 cut(s) 152, 264
BtsCI GGATG 4 cut(s) 46, 61, 336, 452
Cac8I GCNNGC 2 cut(s) 623, 886
CaiI CAGNNNCTG 1 cut(s) 638
Cfr13I GGNCC 2 cut(s) 249, 428
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 3 cut(s) 21, 73, 205
CviAII CATG 5 cut(s) 153, 265, 394, 616, 899
CviQI GTAC 3 cut(s) 21, 73, 205
DdeI CTNAG 5 cut(s) 80, 684, 744, 756, 848
DrdI GACNNNNNNGTC 1 cut(s) 730
DseDI GACNNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 719
EagI CGGCCG 1 cut(s) 719
Eam1104I CTCTTC 4 cut(s) 54, 183, 467, 708
EarI CTCTTC 4 cut(s) 54, 183, 467, 708
Ecl136II GAGCTC 1 cut(s) 64
EclXI CGGCCG 1 cut(s) 719
Eco130I CCWWGG 2 cut(s) 152, 264
Eco24I GRGCYC 4 cut(s) 66, 367, 698, 823
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 249, 428
Eco52I CGGCCG 1 cut(s) 719
Eco53kI GAGCTC 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 991
EcoT14I CCWWGG 2 cut(s) 152, 264
EcoT22I ATGCAT 1 cut(s) 1025
EcoT38I GRGCYC 4 cut(s) 66, 367, 698, 823
ErhI CCWWGG 2 cut(s) 152, 264
Esp3I CGTCTC 1 cut(s) 705
FaeI CATG 5 cut(s) 156, 268, 397, 619, 902
FaqI GGGAC 4 cut(s) 11, 256, 524, 718
FatI CATG 5 cut(s) 152, 264, 393, 615, 898
FbaI TGATCA 1 cut(s) 958
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 587, 719
FokI GGATG 4 cut(s) 33, 68, 343, 459
FriOI GRGCYC 4 cut(s) 66, 367, 698, 823
Fsp4HI GCNGC 2 cut(s) 587, 719
FspBI CTAG 1 cut(s) 945
GluI GCNGC 2 cut(s) 587, 719
GsaI CCCAGC 2 cut(s) 273, 511
GsuI CTGGAG 1 cut(s) 835
HaeIII GGCC 1 cut(s) 721
HapII CCGG 1 cut(s) 33
Hin1II CATG 5 cut(s) 156, 268, 397, 619, 902
HindIII AAGCTT 1 cut(s) 886
HinfI GANTC 9 cut(s) 8, 144, 408, 413, 477, 512, 850, 905, 997
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 884
Hpy166II GTNNAC 3 cut(s) 21, 307, 896
Hpy188I TCNGA 7 cut(s) 51, 125, 177, 418, 664, 745, 910
Hpy8I GTNNAC 3 cut(s) 21, 307, 896
Hpy99I CGWCG 3 cut(s) 55, 71, 557
HpyAV CCTTC 6 cut(s) 274, 354, 437, 497, 796, 863
HpyCH4III ACNGT 3 cut(s) 25, 542, 1007
HpyCH4V TGCA 7 cut(s) 397, 459, 621, 658, 896, 931, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 504, 603, 685
HpyF3I CTNAG 5 cut(s) 80, 684, 744, 756, 848
Hsp92II CATG 5 cut(s) 156, 268, 397, 619, 902
Ksp22I TGATCA 1 cut(s) 958
LguI GCTCTTC 2 cut(s) 54, 183
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 598
MaeI CTAG 1 cut(s) 945
MaeIII GTNAC 2 cut(s) 788, 805
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 5 cut(s) 66, 367, 698, 823, 898
MluCI AATT 2 cut(s) 610, 991
MlyI GAGTC 5 cut(s) 17, 402, 506, 859, 1006
MmeI TCCRAC 4 cut(s) 74, 155, 260, 587
Mph1103I ATGCAT 1 cut(s) 1025
MseI TTAA 4 cut(s) 537, 602, 710, 977
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 266, 504, 603, 685
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 2 cut(s) 152, 264
NlaIII CATG 5 cut(s) 156, 268, 397, 619, 902
NlaIV GGNNCC 2 cut(s) 366, 986
NsiI ATGCAT 1 cut(s) 1025
PaeR7I CTCGAG 1 cut(s) 580
PciSI GCTCTTC 2 cut(s) 54, 183
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 4 cut(s) 144, 413, 477, 905
PkrI GCNGC 2 cut(s) 588, 720
PleI GAGTC 5 cut(s) 16, 402, 506, 858, 1005
PpsI GAGTC 5 cut(s) 16, 402, 506, 858, 1005
Psp124BI GAGCTC 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 269, 507
PspN4I GGNNCC 2 cut(s) 366, 986
PspPI GGNCC 2 cut(s) 249, 428
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 3 cut(s) 22, 74, 206
RsaNI GTAC 3 cut(s) 21, 73, 205
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 2 cut(s) 54, 183
SaqAI TTAA 4 cut(s) 537, 602, 710, 977
SatI GCNGC 2 cut(s) 587, 719
Sau96I GGNCC 2 cut(s) 249, 428
SchI GAGTC 5 cut(s) 17, 402, 506, 859, 1006
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 5 cut(s) 66, 367, 698, 823, 898
SfcI CTRYAG 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 580
SinI GGWCC 2 cut(s) 249, 428
SlaI CTCGAG 1 cut(s) 580
SmlI CTYRAG 3 cut(s) 514, 580, 689
SmoI CTYRAG 3 cut(s) 514, 580, 689
Sse9I AATT 2 cut(s) 610, 991
SsiI CCGC 4 cut(s) 40, 434, 718, 797
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 2 cut(s) 152, 264
TaaI ACNGT 3 cut(s) 25, 542, 1007
TaqI TCGA 8 cut(s) 15, 108, 147, 292, 411, 581, 781, 995
TasI AATT 2 cut(s) 610, 991
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 4 cut(s) 144, 413, 477, 905
Tru1I TTAA 4 cut(s) 537, 602, 710, 977
Tru9I TTAA 4 cut(s) 537, 602, 710, 977
TseI GCWGC 1 cut(s) 586
TspDTI ATGAA 6 cut(s) 35, 72, 201, 604, 882, 918
TspGWI ACGGA 4 cut(s) 68, 541, 581, 657
VneI GTGCAC 1 cut(s) 894
VpaK11BI GGWCC 2 cut(s) 249, 428
XapI RAATTY 2 cut(s) 610, 991
XhoI CTCGAG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 1 cut(s) 945
Zsp2I ATGCAT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.